• Title/Summary/Keyword: phylogenetic

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Sooty Mould Disease Caused by Leptoxyphium kurandae on Kenaf

  • Choi, In-Young;Kang, Chan-Ho;Lee, Geon-Hwi;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
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    • v.43 no.3
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    • pp.347-350
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    • 2015
  • In September 2013, we discovered sooty mould growing on kenaf with the extrafloral nectaries in Iksan, Korea and identified the causative fungus as Leptoxyphium kurandae based on morphological characteristics and phylogenetic analyses. This is the first report of sooty mould caused by L. kurandae on kenaf in Korea and globally.

Marasmioid and Gymnopoid Fungi of the Republic of Korea. 8. Gymnopus Section Levipedes

  • Ryoo, Rhim;Antonin, Vladimir;Ka, Kang-Hyeon
    • Mycobiology
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    • v.48 no.4
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    • pp.252-262
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    • 2020
  • Collections of Gymnopus sect. Levipedes from the Republic of Korea have been studied. Two new species, Gymnopus dryophiloides and G. brunneodiscus, are described based on their macro- and micromorphological and phylogenetic characteristics. Three other species, referred to as Gymnopus spp. 1, 2, and 3, are distinguished as separate taxa without formal descriptions. Taxonomic and phylogenetic positions of all taxa have been inferred and confirmed by analyses of ITS and LSU sequence data. Their detailed descriptions, illustrations and an identification key are provided.

First Report of Arbuscular Mycorrhizal Fungi in Korea: Acaulospora delicata,Dentiscutata colliculosa, and Racocetra alborosea.

  • Park, Hyeok;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.209-215
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    • 2020
  • Spores of arbuscular mycorrhizal fungi (phylum Glomeromycota) were isolated from rhizosphere soil collected in Korea. We identified the morphological characteristics of the spores and performed a phylogenetic analysis using the rDNA 18S, 5.8S, and 28S regions. To the best of our knowledge, we confirm the presence of three species of glomeromycotan fungi previously not reported in Korea, namely Acaulospora delicata, Dentiscutata colliculosa, and Racocetra alborosea. We described the morphological characteristics and results of phylogenetic analysis of these species.

Molecular phylogeny of Daucus (Apiaceae): Evidence from nuclear ribosomal DNA ITS sequences

  • Lee, Byoung Yoon;Park, Chong-Wook
    • Journal of Species Research
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    • v.3 no.1
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    • pp.39-52
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    • 2014
  • The Apiaceae genus Daucus consists of approximately 25 species (including carrots) which are characterized by the presence of bracts in pedunculate umbels, dorsally compressed mericarps, hairs on primary ridges, and uniseriately arranged spines on the secondary ridges of the fruit. Taxonomically, Daucus has been considered to be one of the most problematic genera in the Apiaceae due to the highly variable fruit morphology. Despite taxonomic controversy and economic importance of the genus, no rigorously constructed estimate of phylogenetic relationships exists. To examine generic limit and relationships among species of Daucus and its putatively related taxa, phylogenetic analyses of characters derived from nuclear ribosomal DNA ITS sequences were conducted. Two major clades emerged within Daucus, but neither of them have been previously recognized using morphological characters. The phylogeny also provides taxonomic status of recently reported new species of Daucus, D. arcanus and D. conchitae.

Sequence Comparison of Mitochondrial Small subunit Ribosomal DNA in Penicillium

  • Bae, Kyung-Sook;Hong, Soon-Gyu;Park, Yoon-Dong;Wonjin Jeong
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.62-65
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    • 2000
  • Partial sequence comparisons of mitochondrial small subunit rDNA (mt SSU rDNA) were used to examine taxonomic and evolutionary relationships among seven Penicillium species : two monoverticillate species, two biverticillate species, and three terverticillate species. Amplified fragments of mt SSU rDNA highly varied among seven species in size, suggesting the existence of multiple insertions or deletions in the region. A phylogengtic tree was constructed by exhaustive search of parsimony analysis. The phylogenetic tree distinguished two statistically supported monophyletic groups, one for two monoverticillate species and the other for three terverticillate species and ont biverticillate species, P. vulpinum. The phylogenetic relationship of P. waksmanii, the biverticillate species, was not clear.

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First Record of the Himalayan Swiftlet Aerodramus brevirostris (Aves: Apodiformes) from Korea

  • Choi, Chang-Yong;Park, Jong-Gil;Lee, Yun-Sun;Min, Mi-Sook;Bing, Gi-Chang;Hong, Gil-Pyo;Lee, Hang
    • Animal Systematics, Evolution and Diversity
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    • v.25 no.3
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    • pp.269-273
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    • 2009
  • On 28 April 2008, a male Himalayan Swiftlet (Aerodramus brevirostris) was captured and examined at a night roost of swallows on Hongdo Island, Jeollanam-do, Korea. This is the first record of this species from Korea confirmed by specimen examination. We describe morphological features and some phylogenetic notes of the Himalayan Swiftlet found.

Cloning and Phylogenetic Analysis of Chitin Synthase Gene from Entomopathogenic Fungus, Beauveria brongniartii

  • Nam, Jin-Sik;Lee, Dong-Hun;Park, Ho-Yong;Bae, Kyung-Sook
    • Journal of Microbiology
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    • v.35 no.3
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    • pp.222-227
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    • 1997
  • DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHSl. Comparison of the deduced amino acid sequence of BbCHSl with those of other Euascomycetes revealed that BbCHSl is a gene for class II chitin synthase. The Blastp search of the deduced amino acid sequence of BbCHSl displayed the highest rate of similarity, 95.8%, with CHS2 of Metarhizium unisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongniartii, which was previously derived by traditional fungal classification based on morphological features.

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Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani

  • Bae, Young-An;Kong, Yoon
    • Parasites, Hosts and Diseases
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    • v.41 no.4
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    • pp.221-231
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    • 2003
  • To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.

Sirtuin/Sir2 Phylogeny, Evolutionary Considerations and Structural Conservation

  • Greiss, Sebastian;Gartner, Anton
    • Molecules and Cells
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    • v.28 no.5
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    • pp.407-415
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    • 2009
  • The sirtuins are a protein family named after the first identified member, S. cerevisiae Sir2p. Sirtuins are protein deacetylases whose activity is dependent on $NAD^+$ as a cosubstrate. They are structurally defined by two central domains that together form a highly conserved catalytic center, which catalyzes the transfer of an acetyl moiety from acetyllysine to $NAD^+$, yielding nicotinamide, the unique metabolite O-acetyl-ADP-ribose and deacetylated lysine. One or more sirtuins are present in virtually all species from bacteria to mammals. Here we describe a phylogenetic analysis of sirtuins. Based on their phylogenetic relationship, sirtuins can be grouped into over a dozen classes and subclasses. Humans, like most vertebrates, have seven sirtuins: SIRT1-SIRT7. These function in diverse cellular pathways, regulating transcriptional repression, aging, metabolism, DNA damage responses and apoptosis. We show that these seven sirtuins arose early during animal evolution. Conserved residues cluster around the catalytic center of known sirtuin family members.

Pythium subutonaiense, A New Aquatic Oomycete from Southern China Based on Morphological and Molecular Characters

  • Chen, Jia-Jia;Zheng, Xiao-Bo
    • Mycobiology
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    • v.47 no.3
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    • pp.273-279
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    • 2019
  • A new species, Pythium subutonaiense, isolated from aquatic environments (lake) in China is being described based on morphological characters and molecular evidence. The isolates grew at temperatures between $5^{\circ}C$ and $38^{\circ}C$, and the optimum temperature was $30^{\circ}C$, with a radial growth rate of 17.6 mm at $25^{\circ}C$ per day. It is homothallic and characterized by globose to sub-globose shaped and mostly terminal or sometimes catenulate hyphal swellings, filamentous non-inflated sporangia, and smooth oogonia with hypogynous and monoclinous antheridia that contained one plerotic oospore. In phylogenetic analysis, inferred based on the internal transcribed spacer region of the ribosomal RNA gene and mitochondrial cytochrome c oxidase subunit 1 gene, the new species formed a distinct lineage in Pythium clade B. Differences between the new species and phylogenetically related and morphologically similar species are discussed.