• Title/Summary/Keyword: phycocyanin

Search Result 80, Processing Time 0.029 seconds

Quality Characteristics of Tofu Prepared with Spirulina (스피루리나 첨가 두부의 품질특성)

  • Kim, Hyo-Jin;Lee, Jee-Yeon;Lee, Seung-Hee;Lee, Kun-Jong;Kim, Mee-Ree
    • Korean journal of food and cookery science
    • /
    • v.26 no.6
    • /
    • pp.887-893
    • /
    • 2010
  • In this study, the quality characteristics of tofu prepared with added spirulina(0, 0.25, 0.5, 0.75, and 1%). As the concentration of spirulina increased, tofu yield decreased and the pH and turbidity increased. The Hunter L(lightness) and a(redness) values of tofu decreased as the amount of spirulina increased. Phycocyanin(C-phycocyanin, allophycocyanin and phycoerythrin) contents increased according to the spirulina amount. Moreover the antioxidant activity of tofu increased as the concentration of spirulina increased($IC_{50}$ values for control was 150.7 mg/g; 1% spirulina, 82.5 mg/g). Textural properties(TPA) for hardness, gumminess, and chewiness were higher in spirulina with added tofu, compared to those of control. Regarding the overall acceptability of sensory properties, spirulina with 0.5% added tofu had the highest scores among all treatments. From these results, it was suggested that the optimal amount of added spirulina was 0.5% for the preparation of high quality tofu.

Studies on the Isolation of Phycobiliprotein from S. platensis (S. platensis로부터 phycobiliprotein의 분리에 관한 연구)

  • Kim Jum-Ji;Kim Yoon-Kyoun;Lee Mi-Young
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.5 no.5
    • /
    • pp.484-489
    • /
    • 2004
  • Isolation of phycobiliprotein from S. platensis was performed by using $30-60{\%}$ ammomium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-Sephacel anionic exchange chromatography. Isolated phycobiliprotein was determined to be a c-phycocyanin with a maximum absorption wavelength at 620 nm. This phycobiliprotein consisted of $({\alpha}$ and $({\beta}$ subunit when analyzed through SDS-PAGE. The molecular weights of $({\alpha}$ and $({\beta}$ subunit were 14.5 kDa and 16 kDa, respectively. The native molecular weight of phycobiliprotein through gel filtration was about 100 kDa. These results show that the structure of phycobiliprotein from S. platensis might be aggregated form of $({\alpha}{\beta})_{3}-trimer$.

  • PDF

A study on the classification of Algae species using hyperspectral sensors (초분광센서를 활용한 조류종 구분에 관한 연구)

  • Kim, Gwang Soo;Nam, Su Han;Gwon, Weong Hwa;Kim, Dong Soo;Kim, Young Do;Kwon, Jae Hyun
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2022.05a
    • /
    • pp.87-87
    • /
    • 2022
  • 최근 국내외 이상기후 변화로 인해 일사량 증가 및 하천, 호소의 체류시간이 늘어남에 따라 조류의 과대성장이 빈번히 발생하고 있다. 하천의 조류는 크게 녹조류, 남조류, 규조류로 분류되며, 남조류 중 일부 종은생장하는 과정에서 생성하는 냄새물질, 독성물질의 배출로 문제를 야기하고 있다. 국민의 안전을 도모하기 위해 국내에서는 조류경보제, 수질예보제를 시행하고 있으며, 이는 유해남조류세포와 Chl-a농도에 따라 발령이 된다. 이렇듯 유해남조류와 Chl-a 측정은 매우 중요하며, 현재 조류의 분석방법은 현장샘플을 하여 조류검경을 통해 산정하기 때문에 시간이 많이 소요되며, 조류발생시 즉각대응이 힘들며, 육안으로 유해남조류인지 구별하기가 어렵다. 최근 원격탐사를 통해 조류의 대체 인자인 Chl-a, Phycocyanin을 통해 조류농도를 분석하고 있으나, 광합성을하는 모든 조류에는 Chl-a가 함유되어 있어 남조류와 녹조류의 정확히 구별하는데 한계점이 있다. 본 연구에서는 조류배양액을 초분광센서를 통해 조류스펙트럼을 취득하고, 남조류와 녹조류의 최대 흡수 스펙트럼을 추출하여 스펙트럼의 순간기울기 변화를 통해 남조류와 녹조류의 종분류하였다.

  • PDF

Improvement of Model based on Inherent Optical Properties for Remote Sensing of Cyanobacterial Bloom (고유분광특성을 이용한 남조류 원격 추정 모델 개선)

  • Ha, Rim;Nam, Gibeom;Park, Sanghyun;Kang, Taegu;Shin, Hyunjoo;Kim, Kyunghyun;Rhew, Doughee;Lee, Hyuk
    • Korean Journal of Remote Sensing
    • /
    • v.33 no.2
    • /
    • pp.111-123
    • /
    • 2017
  • The phycocyanin pigment (PC) is a marker for cyanobacterial presence in eutrophic inland water. Accurate estimation of low PC concentration in turbid inland water is challenging due to the optical complexity and criticalforissuing an early warning of potentialrisks of cyanobacterial bloom to the public. To monitor cyanobacterial bloom in eutrophic inland waters, an approach is proposed to partition non-water absorption coefficient from measured reflectance and to retrieve absorption coefficient of PC with the aim of improving the accuracy in remotely estimated PC, in particular for low concentrations. The proposed inversion model retrieves absorption spectra of PC ($a_{pc}({\lambda})$) with $R^2{\geq}0.8$ for $a_{pc}(620)$. The algorithm achieved more accurate Chl-a and PC estimation with $0.71{\leq}R^2{\leq}0.85$, relative root mean square error (rRMSE) ${\leq}39.4%$ and mean relative error(RE) ${\leq}78.0%$ than the widely used semi-empirical algorithm for the same dataset. In particular, low PC ($PC{\leq}50mg/m^3$) and low PC: Chl-a ratio values of for all datasets used in this study were well predicted by the proposed algorithm.

Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

  • Dai, Yu-Jie;Li, Jing;Wei, Shu-Mei;Chen, Nan;Xiao, Yu-Peng;Tan, Zhi-Lei;Jia, Shi-Ru;Yuan, Nan-Nan;Tan, Ning;Song, Yi-Jie
    • Journal of Microbiology and Biotechnology
    • /
    • v.23 no.4
    • /
    • pp.534-538
    • /
    • 2013
  • The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under $27{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity and $25^{\circ}C$ on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

제주도산 갈래곰보의 색소안정성 및 다당류 조성

  • ;;;A.V. Podkorytova;I.A. Kadnikova;S.V. Suchoverkchov
    • Proceedings of the Korean Society of Fisheries Technology Conference
    • /
    • 2001.05a
    • /
    • pp.87-88
    • /
    • 2001
  • 아열대성 홍조류인 갈래곰보는 해수온도가 12-13$^{\circ}C$ 이상을 유지하는 제주도 남쪽해안에서만 자생하고 있다. 홍조류에는 phycoerythrin, phycocyanin 및 allophycocyanin 색소성분을 함유하고 있으며, 이러한 성분은 품종이나 색소추출방법에 따라 최대습수 파장이 다소 차이가 나타나며, 흡광도에 의해서 색소의 특징을 나타내게 한다. (중략)

  • PDF

Geosmin Concentration and Its Relation to Environmental Factors in Daechung Reservoir, Korea (대청호의 geosmin 농도와 환경요인과의 관계)

  • Park, Dae-Kyun;Maeng, Jue-Son;Ahn, Chi-Yong;Chung, An-Sik;Lee, Jin-Hwan;Oh, Hee-Mock
    • Korean Journal of Ecology and Environment
    • /
    • v.34 no.4 s.96
    • /
    • pp.319-326
    • /
    • 2001
  • The biological and physicochemical factors and geosmin concentration were monitored in the Daechung Reservoir for 25 weeks from April to October in 1999. Geosmin was detected 5 times within a range of $1.16{\sim}5.28\;ng\;L^{-1}$, mostly in the late summer. The highest geosmin concentration was recorded on August 31, which also overlapped with the peaks of phycocyanin concentration, cyanobacterial density, and Anabaena spiroides density. A correlation analysis indicated that geosmin production was closely related with a high water temperature, pH, total dissolved phosphorus (TDP), and A. spiroides density. A water temperature of $27^{\circ}^C$, pH of 8.5, and TDP of $0.06\;mg\;L^{-1}$ were identified as the prerequisite environmental condition sand threshold values for geosmin production. Accordingly, under such conditions, an A. spiroides density above $10,000\;cells\;mL^{-1}$ indicates imminent geosmin occurrence.

  • PDF

Optimizing the bio-optical algorithm for quantifying Chlorophyll-a and Phycocyanin in inland water, Korea (대한민국 담수계의 클로로필a와 피코시아닌 정량화를 위한 분광알고리즘 최적화 연구)

  • Pyo, JongCheol;Pachepsky, Yakov;Lee, Hyuk;Park, Yongeun;Cho, Kyung Hwa
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2017.05a
    • /
    • pp.101-101
    • /
    • 2017
  • 근래에 대한민국 담수계에 조류 대발생으로 인한 수질악화 문재가 대두되고 있다. 또한 독성물질을 생성하는 남조류종이 우점하는 현상으로인해 수질문제와더불에 생태계와 인간의 건강도 잠재적인 위험을 받고있는 실정이다. 이와같은 조류 대발생으로인한 피해를 최소화하기위해 효과적인 수질관리가 필수적이다. 원격탐사기술은 조류의 공간적인 분포를 해석하고 농도를 정량화하기위해 이용되고 있다. 현재까지 많은 분광알고리즘들이 개발되어 담수유역에 적용이 되고 있다. 수체마다 다른 분광특성 때문에 알고리즘내의 파라미터 및 분광밴드 조정이 필수적이다. 하지만 대부분의 연구에선 파라미터와 밴드의 변경에 따른 결과향상에만 초점이 맞춰지고 있어 분광알고리즘내의 파라미터와 분광밴드사이의 관계 이해 뿐만아니라 알고리즘 최종 산출물에 대한 영향에 관한 설명이 전무한 실정이다. 본 연구에선, 대한민국 백제보를 대상으로 현장모니터링 및 조류추출 실험을 진행하였고, 이를 기반으로 5가지 클로로필a 알고리즘과 2가지 피코시아닌 알고리즘을 구축하였다. 알고리즘내에서 변수들의 관계와 영향을 알아보기위해 민감도 분석을 실시하였다. 민감도 분석 조건을 기반으로 one-objective 최적화 및 multi-objective 최적화를 실시하여 백제보수계를 대표할 수 있는 최적 변수들을 모의하였다. 민감도 분석결과 후방산란계수에 영향을 미치는 파라미터와 조류 생체량에 영향을 미치는 파라미터가 다른 변수들 및 알고리즘 농도산정결과에 가장 민감한 것으로 나타났다. multi-objective 최적화 결과가 one-objective 결과 및 reference 결과보다 대부분 정확도가 향상되었고 흡광도 계수를 함께 고려할 수 있기 때문에 백제보 수계의 분광특성을 함께 고려하여 대표할 수 있는 장점을 가지는 것으로 나타났다. 따라서, 본 연구는 민감도 분석을 활용하여 분광알고리즘 내의 변수들의 이해를 도모하였고, 최적화 기법 중, multi-objective 최적화 기법이 백제보의 분광특성을 대변하는 최적변수를 제시할 수 있음과 동시에 보다 나은 정확성을 제고할 수 있음을 확인하였다.

  • PDF

Effects of biostimulants, AMPEP and Kelpak on the growth and asexual reproduction of Pyropia yezoensis (Bangiales, Rhodophyta) at different temperatures

  • Sook Kyung Shin;Qikun Xing;Ji-Sook Park;Charles Yarish;Fanna Kong;Jang K. Kim
    • ALGAE
    • /
    • v.39 no.1
    • /
    • pp.31-41
    • /
    • 2024
  • Acadian marine plant extract powder (AMPEP) and Kelpak are commercial biostimulants derived from brown algae Ascophyllum nodosum. This study was to determine if AMPEP and Kelpak can induce thermal resistance in Pyropia yezoensis. P. yezoensis blades were exposed to different concentrations (control: 0, low: 0.001, high: 1 ppm) of AMPEP and Kelpak at 10℃ for 6 and 7 days, respectively. Those blades were then cultivated in von Stosch enriched seawater medium at different temperatures (10, 15, 20, and 25℃) with 12 : 12 L : D photoperiod and 100 µmol m-2 s-1 of photosynthetically active radiation for additional 15 days. Results showed that P. yezoensisreproduced archeospores at 20 and 25℃ at all biostimulant conditions within 15 days. At lower temperatures (10 and 15℃), only AMPEP-treated P. yezoensis reproduced archeospores. P. yezoensis exposed to 1 ppm Kelpak exhibited higher phycoerythrin and phycocyanin contents than control and 0.001 ppm conditions at 15℃. AMPEP-treated conditions showed higher phycoerythrin and phycocyanin contents than control at 10℃. These results suggest that AMPEP and Kelpak may not enhance the thermal resistance of P. yezoensis. However, AMPEP stimulated archeospores release at lower temperatures. The treatment of AMPEP and Kelpak also increased the pigment contents in P. yezoensis. These results suggest that the use of seaweed-derived biostimulants can provide some economic benefits in P. yezoensis aquaculture. The enhancement of archeospores formation by AMPEP at lower temperature may also increase the productivity since Pyropia farming relies on the accumulation of secondary seedings via asexual reproduction.

Pigment Stability of Lavers Porhyra tenera Kjellman during Processing and Storage (김의 가공 저장중에 있어서의 색소의 안정도)

  • LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.2 no.2
    • /
    • pp.105-133
    • /
    • 1969
  • The stability of three major pigments in lavers, namely chlorophylls, carotenoids, and phy-cobilins, during processing and storage was studied. The results of the pigment retention in fresh lavers during storage at low temperatures, of heat lability during heat treatment, and of the pigment loss during storage are summarized as follows: 1. The contents of chlorophyll a, xanthophyll (lutein+zeaxanthin), and carotene ($\alpha-+\beta-carotene$) averaged 1,525, 627, and $409{\mu}g/g$ of dry samples respectively The comparative composition of four carotenoids, lutein, $\beta-carotene$, zeaxanthin, and $\alpha-carotene$, was 35.9, 33.7, 12.2, and $5.8\%$ respectively. 2. In storage of fresh lavers at room ternperature ($20-25^{\circ}C$), 2 to $5^{\circ}C$, and $-15^{\circ}C$, the pigments generally were retained more at 2 to $5^{\circ}C$ than others while carotene only showed more retention at $-15^{\circ}C$ than at 2 to $5^{\circ}C$. Phycocyanin tended to decrease more rapidly than phyco-erythrin at low temperatures. 3. In regard to heat treatment of both dried and wet materials at 40, 60, 80, $100^{\circ}C$ respectively, the pigments were more stable at lower temperatures than at higher temperatures in both cases of materials. Carotenoids were retained more in wet materials than in the sun dried. Xanthophylls showed higher heat stability than carotenes. Phycoerythrin was considered more heat labile than phycocyanin, particularly at higher temperatures. 4. In storage of heat treated lavers for 50 days at room temperature, the retention of pigments appeared differently in accordance with the temperatures of treatment The materials treated at $60^{\circ}C$ showed better relention of pigments than those treated at the other temperatures. Degradation rate of xanthopllylls, carotenes, and phycobilins was decreased in the materials treated at higher temperatures, showing a tendency of great pigment retention even for a long period storage. Chlorophyll il was retained more in sun dried materials whereas xanthophyll and carotene were retained more in the wet. 5. In order to maintain a higher pigment stability during storage, the materials must be treated at $60^{\circ}C$ in a dryer with rapid ventilation until the moisture level reaches about $6.0\%$. In case of treatment at a higher temperature than $60^{\circ}C$, they must be treated for a short period of time to minimize pigment degradation by heat. 6. The lavers treated with the solutions of pH around 7.8 showed a higher retention of chlorophyll a. When treated with the solutions of algal extracts such as agar and alginic acid, uniform layer of laver sheets and better surface gloss of dried layers were obtained.

  • PDF