• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.9-21
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• 2005

To study the biochemical and physiological role of the plastidic glucose transporter (pGlcT) in carbohydrate metabolism, we characterized transgenic plants with mutations in the pGlcT gene (GT), gt-1 and gt-2, as well double mutants of GT and the maltose transporter (MEX1) and GT and the triose phosphate/phosphate translocator (TPT), GT and the cytosolic fructose-1,6-bisphosphatase gene (cFBP), and MEX1 and TPT, gt-1/mex2, gt-1/tpt-2, gt-1/cfbp-1, mex1-1/tpt-2, respectively. Compared to the wild type, all mutants except the gt-1/cfbp-1 mutant lines displayed higher starch accumulation and higher levels of maltose. Starch accumulation is due to a decrease in starch turnover, leading to an imbalance between the rates of synthesis and degradation. Sucrose levels of gt alleles were higher than those in wild-type plants during the light period, suggesting possible nightly supplementation via the maltose transport pathway to maintain proper carbohydrate partitioning in the plant leaves. The gt plants displayed less growth retardation than mex1-1 mutant and gt-1/mex2 double mutant displayed accumulativesevere growth retardation as compared to individual gt-1 and mex1-1 mutants, implying that the maltose transporter-mediated pathway is a major route for carbohydrate partitioning at night. The gt-1/tpt-2, mex1-1/tpt-2 and gt-1/cfbp-1 double mutants had retarded growth and low chlorophyll content to differing degrees, indicating that photosynthetic capacity had diminished. Interestingly, the gt-1/tpt-2 line displayed a glucose-insensitive phenotype and higher germination rates than wild type, suggesting its involvement not only in carbon partitioning, but also in the sugar signaling network of the pGlcT and TPT.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.165-172
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• 2003

The objectives of this study were to examine the physiological responses to ozone and to measure ozone uptake rates of Betula species exposed to relatively high concentration of pollutants. At the end of the growing season, photosynthesis, pigments contents, antioxidants (SOD and GR) and ozone uptake rates were measured or estimated at the leaves of five Betula species (Betula costata, B. davurica, B. platyphylla var. japonica, B. schmidtii and B. ermanii) exposed to 100ppb ozone concentration. On the termination of the experiment, growth effects were determined by measuring leaf area and dry weights of leaf, stem and root. Ozone treatment showed the significant reduction the leaf area and dry weight of four Betula species, except for B. ermanii. Shoot / root (SR) ratio of five species represented two different types. SR ratio of B. costata and B. davurica were lower than control, in contrast, SR ratio of B. platyphylla var. japonica, B. schimidtii and B. emani, were higher than that of control. The photosynthetic responses of five species were different in responses to ozone exposure. Four species, except for B. emanii, maintained or increased the stomatal conductance, but B. emanii decreased both stomatal conductance and photosynthesis. SOD activities of five species decreased by the ozone exposure, especially B. ermanii showed the largest reduction, GR activities of B. platyphylla var. japonica and B. schmidtii increased, B. costata and B. emanii decreased. Instantaneous ozone uptake rate was the highest at the leaves of B. ermanii and B. costata, ozone uptake per seedling was the highest at the leaf of B. schmidtii and B. emanii. It was concluded that B. costata, B. davurica and B. platyphylla var. japonica, appeared the growth reduction and visible ozone injury, were sensitive species to ozone, and B. schmidtii with the increased antioxidant activity and B. ermanii without the growth reduction were relatively resistant species to high ozone concentration at the early growing stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.2
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• pp.126-130
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• 2004

This study is to identify the physiological traits of submergence-tolerant varieties of rice plants (Oryza sativa L.) in Yeongnam area, southeastern part of Korea, where the reduction of rice yield due to submergence is remarkably severe. In the present study, two tolerant varieties of rice plants were selected from over 30 rice varieties grown in under a 10-day period. The tolerant varieties selected from a submerged paddy field. As a control, one intolerant variety of rice plant was chosen. Of the tolerant variety Samgangbyeo, rather than Haepyungbyeo, had a lower dissolved oxygen consumption and maintained a higher dry weight than the intolerant variety. The leaf photosynthetic rates (LPS) of the two tolerant varieties were significantly higher than that of the intolerant-variety after four days of submergence treatment. These results indicate that lower dissolved oxygen consumption in a limited pool is prevented by ethylene formation in the tolerant varieties, which may be a mechanism of submergence tolerance.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.247-258
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• 2004

Climatological, hydrological and planktonical research studies, measurements of primary production and photosynthetic efficiency from December 1976 to December 1978 have been carried out in two brackish lakes: Lake Etang de Berre and Lake Etang de Vaine located in the French Mediterranean coast, in the region of Carry-le-Rouet located on the north-west Mediterranean near Marseilles, and in fresh water inflows from 4 Rivers (Touloubre, Durance, Arc, Durancole) to Lake Etang de Berre. Physico-chemical parameters were measured for this study: water temperature, salinity, density, pH, alcalinity, dissolved oxygen (% saturation), phosphate, nitrate, nitrite, silicate etc. Diverse biological parameters were also studied: photosynthetic pigments, phaeopigments, specific composition and biomass of phytoplankton, primary pelagic production etc. Climatical factors were studied: air-temperature, solar-radiation, evaporation, direction (including strength) of winds, precipitation and freshwater volume of the four rivers. The changes in Lake ‘Etang de Berre’ ecosystem depend on the quality of the water in the Durance River, and on the effects of seawater near the entrance of the Caronte Canal. The water quality of the lake varies horizontally and vertically as a result of atmospheric phenomena, maritime currents and tides. The distribution of water temperatures is generally heterogeneous. Southeasterly winds and the Northeasterly Mistral wind are important in the origins of circulated and mixed water masses. These winds are both frequent and strong. They have, as a result, a great effect on the water environment of Lake Etang de Berre. In theory, the annual precipitation in this region is well over eight times the water mass of the lake. The water of the Durance River flows into Lake Etang de Berre through the EDF Canal, amounting to 90% of the precipitation. However, reduction of rainfall in dry seasons has a serious effect on the hydrological characteristics of the lake. The temperature in the winter is partially caused by the low temperature of fresh water, particularly that of the Durance River. The hydrological season of fresh and brackish water is about one month ahead of the hydrological season of sea water in its vicinity. The salinity of Lake Etang de Berre runs approximately 3$\textperthousand$, except at lower levels and near the entrance to the Caronte Canal. However, when the volume of the Durance River water is reduced in the summer and fall, the salinity rises to 15$\textperthousand$. In the lake, the ratio of fresh water to sea water is six to one (6:1). The large quantities of seston conveyed by rivers, particularly the Durance diversion, strongly reduce the transparency in the brackish waters. Although the amount of sunshine is also notable, transparency is slight because of the large amount of seston, carried chiefly by Tripton in the fresh water of the Durance River. Therefore, photosynthesis generally occurs only in the surface layer. The transparency progressively increases from freshwater to open seawater, as mineral particles sink to the bottom (about 1.7kg $m^{-2}a^{-1}$ on the average in brackish lakes). The concentration of dissolved oxygen and the rate of oxygen saturation in seawater (Carry-le-Rouet) ranged from 5.0 to 6.0 $m\ell$ㆍ.$1^{-1}$, and from 95 to 105%, respectively. The amount of dissolved oxygen in Etang de Berre oscillated between 2.9 and 268.3%. The monographs of phosphate, nitrate, nitrite and silicate were published as a part of a study on the ecology of phytoplankton in these environments. Horizontal and vertical distributions of these nutriments were studied in detail. The recent diversion of the Durance River into Lake Etang de Berre has effected a fundamental change in this formerly marine environment, which has had a great impact in its plankton populations. A total of 182 taxa were identified, including 111 Bacillariophyceae, 44 Chlorophyceae, and 15 Cyanophyceae. The most abundant species are small freshwater algae, mainly Chlorophyceae. The average density is about $10^{8}$ cells $1^{-1}$ in Lake Etang de Berre, and about double that amount in Lake Etang de Vaine. Differences in phytoplankton abundance and composition at the various stations or at various depths are slight. Cell biovolume V (equivalent to true biomass), plasma volume VP (‘useful’ biomass) and, simultaneously. the cell surface area S and S/V ratio through the measurement of cell dimensions were computed as the parameters of phytoplankton productivity and metabolism. Pigment concentrations are generally very high on account of phytoplankton blooms by Cyanophyceae, Chlorophyceae and Cryptophyceae. On the other hand, in freshwaters and marine waters, pigment concentrations are comparatively low and stable, showing slight annual variation. The variations of ATP concentration were closely related to those of chlorophyll a and phytoplankton blooms only in marine waters. The carbon uptake rates ranged between 38 and 1091 mg$Cm^{-2}d^{-1}$, with an average surface value of 256 mg; water-column carbon-uptake rates ranged between 240 and 2310 mg$Cm^{-2}d^{-1}$, with an average of 810, representing 290 mg$Cm^{-2}$, per year 45 000 tons per year of photosynthetized carbon for the whole lake. Gross photosynthetic production measured by the method of Ryther was studied over a 2-year period. The values obtained from marine water(Carry-le-Rouet) ranged from 23 to 2 337 mg$Cm^{-2}d^{-1}$, with a weighted average of 319, representing about 110 gCm$^{-2}$ per year. The values in brakish water (Etang de Berre) ranged from 14 to 1778 mg$Cm^{-2}d^{-1}$, with a weighted average of 682, representing 250 mg$Cm^{-2}$ per year and 38 400 tons per year of photosynthesized carbon for the whole lake.

• Asian Journal of Atmospheric Environment
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• v.6 no.4
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• pp.259-267
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• 2012

To clarify the effects of black carbon (BC) particles on growth and gas exchange rates of Asian forest tree species, the seedlings of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica were exposed to BC particles with sub-micron size for two growing seasons from 1 June 2009 to 11 November 2010. The BC particles deposited after the exposure to BC were observed on the foliar surface of the 4 tree species. At the end of the experiment, the amount of BC accumulated on the foliar surface after the exposure to BC aerosols were 0.13, 0.69, 0.32 and 0.58 mg C $m^{-2}$ total leaf area in F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings, respectively. In August 2010, the exposure to BC particles did not significantly affect net photosynthetic rate under any light intensity, stomatal diffusive conductance to water vapour ($g_s$), stomatal limitation of photosynthesis, response of $g_s$ to increase in vapour pressure deficit and leaf temperature under light saturated condition in the leaves or needles of the seedlings. These results suggest that the BC particles deposited on the foliar surface did not reduce net photosynthesis by shading, did not increase leaf temperature by absorption of irradiation light, and did not induce plugging of stomata in the leaves or needles of the seedlings. There were no significant effects of BC particles on the increments of plant height and stem base diameter during the experimental period and the whole-plant dry mass at the end of the experiment. These results indicate that the exposure to BC particles with sub-micron size for two growing seasons did not significantly affect the growth and leaf or needle gas exchange rates of F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.401-409
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• 2021

This study aimed to estimate the photosynthetic capacity of tomato plants grown in a semi-closed greenhouse using temperature response models of plant photosynthesis by calculating the ribulose 1,5-bisphosphate carboxylase/oxygenase maximum carboxylation rate (V_cmax), maximum electron transport rate (J_max), thermal breakdown (high-temperature inhibition), and leaf respiration to predict the optimal conditions of the CO₂-controlled greenhouse, for maximizing the photosynthetic rate. Gas exchange measurements for the A-C_i curve response to CO₂ level with different light intensities {PAR (Photosynthetically Active Radiation) 200µmol·m^-2·s^-1 to 1500µmol·m^-2·s^-1} and leaf temperatures (20℃ to 35℃) were conducted with a portable infrared gas analyzer system. Arrhenius function, net CO₂ assimilation (A_n), thermal breakdown, and daylight leaf respiration (R_d) were also calculated using the modeling equation. Estimated J_max, A_n, Arrhenius function value, and thermal breakdown decreased in response to increased leaf temperature (> 30℃), and the optimum leaf temperature for the estimated J_max was 30℃. The CO₂ saturation point of the fifth leaf from the apical region was reached at 600ppm for 200 and 400µmol·m^-2·s^-1 of PAR, at 800ppm for 600 and 800µmol·m^-2·s^-1 of PAR, at 1000ppm for 1000µmol of PAR, and at 1500ppm for 1200 and 1500µmol·m^-2·s^-1 of PAR levels. The results suggest that the optimal conditions of CO₂ concentration can be determined, using the photosynthetic model equation, to improve the photosynthetic rates of fruit vegetables grown in greenhouses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.101-108
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• 2014

This study was conducted to investigate the effect of nitrogen rates on the growth characteristics and yield of maize in agricultural fields with the stream. This indicates the necessity and optimal level of nitrous fertilization to examine the possibilities of quantity enhancement. Plant height and ear height of maize were not significantly different among the nitrogen rates. Stem diameter and leaf area index increased in the nitrogen treatment compared to untreated control. Changes of photosynthetic rate in maize leaves depending on nitrogen treatments increased as much as nitrogen rates were increased up to the highest level, 36 kg per 10a. NDF and ADF content levels of maize were investigated with different nitrogen rates regardless of treatments. In the case of NDF, it showed a tendency to decrease after 8 days of tasseling date. ADF had also decreased after 15 days of tasseling date. Nitrogen uptake of maize leaves with different nitrogen rates showed the highest level, $4.9g\;kg^{-1}$ with 36 kg per 10a on the tasseling date. Ear length and 100-kernel weight, there were no significant differences according to yield and the components with different nitrogen rates. Ear diameter and kernel number, nitrogen rates of 18 kg and 36 kg were increased compared to nitrogen rate of 9 kg per 10a and untreated control. The pericarps in 9 kg nitrogen rate and control were thicker than those of 18 kg and 36 kg treatment. The yield, 18 kg, 36 kg, and 9 kg treatments were increased by 10.96%, 9.27%, and 3.31%, compared to control. The component analysis on maize kernel with different nitrogen rates, starch showed no significant differences among treatments. Total sugar in 18 kg nitrogen treatment represented the highest content level, 6.37%. In addition, Amylopectin in 18 kg treatment showed the highest content level of 90.38%. However, amylose in 18 kg treatment showed the lowest level, 9.62% which drew a conclusion that waxy of 18 kg treatment is considered to be the strongest one. From the results described above, nitrous fertilization is essential to grow maize in agricultural fields with the stream. The optimum level of nitrous fertilization is considered 18 kg per 10a.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.719-726
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• 2016

The effect of high and low temperatures on seedling quality while raising of paprika (Capsicum annuum L.) seedlings, and their early development after planting was investigated. The control raising seedling temperature (RST) was $23^{\circ}C$; high temperature, $31^{\circ}C$; and low temperature, $15^{\circ}C$ throughout the raising seedling period. At $15^{\circ}C$ and $30^{\circ}C$, plant height, stem diameter, fresh weight, dry weight, number of leaves, and seedling leaf area were significantly lower than those at $23^{\circ}C$. At 4 weeks after planting, seedling's growth characteristics showed a similar pattern. Compared to $23^{\circ}C$, seedlings raised at $15^{\circ}C$ and $30^{\circ}C$ had an increased dry weight and leaf area per unit time after planting than during the seedling raising period. At 4 weeks after planting, crop growth rate and leaf area index were unaffected by RST, and relative growth rate and net assimilation rate at RSTs of $15^{\circ}C$ and $31^{\circ}C$ were higher than those those at RST of $23^{\circ}C$. At an RST of $15^{\circ}C$, growth speed and net assimilation rates were higher after planting than before planting, according to increased photosynthetic rate. Thus, high and low temperatures during the seedling raising period significantly reduced seedling growth and plant growth after planting. After planting, seedlings raised at $15^{\circ}C$ recovered more quickly than did those raised at $31^{\circ}C$.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.535-539
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• 2010

The formation and elongation of runners, growth of runner plants, and transplant propagation rates of 'Maehyang' strawberry were investigated at various photosynthetic photon flux (PPF) levels. Strawberry plants having $3.1{\pm}0.4$ leaves and $7.0{\pm}1.1mm$ of crown diameter were used as propagules and were cultured for 35 days in 9 transplant production modules using fluorescent lamps as artificial lighting sources. Applied PPF levels were $137.4{\pm}2.1$, $217.0{\pm}1.0$, and $274.7{\pm}8.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as measured on the surfaces of empty shelves. The numbers of runners and runner plants per propagule were the greatest at $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF. The runner plant propagation rate was 0.27 plant/day/propagule at $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, which was significantly greater than that of conventional propagation methods. Results indicate that high PPF levels promotes the formation of runners and runner plants of strawberry and that the rapid propagation method with high PPF levels can be feasible for production of vigorous transplants in a closed transplant production system.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.9-16
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• 1995

The effect of day and night temperature on the seedlings growth as well as physiological responses of red pepper seedlings to temperature, such as uptake of water and nutrients, rates of photosynthesis and respiration of leaf and root were also investigated in growth cabinet. The results obtained were as follows ; 1. As the temperature dropped down to 12$^{\circ}C$, the uptake of water and nutrients, nitrate, phosphorus and potassium were decreased drastically. At 5$^{\circ}C$ there was virtually no uptake of water and nutrients. 2. Photosynthetic activity in the leaves of red pepper seedlings was increased gradually from 5$^{\circ}C$ to $25^{\circ}C$ and observed the highest photosynthetic activity at $25^{\circ}C$, but respiratory activity of leaf increased up to 3$0^{\circ}C$ and the same trend was observed in root respiratory activity. 3. Optimal combination of day and night temperature for shoot dry weight which is the decisive criterion of good seedlings of red pepper was found to be $25^{\circ}C$ at nighttime and 3$0^{\circ}C$ at daytime and then day/night temperature showed in the order of 25/25, 30/15, 15/25, 10/$25^{\circ}C$. No increment of shoot dry weight at 5$^{\circ}C$ in nighttime temperature observed regardless of daytime temperature.