• KSBB Journal
• /
• 제22권5호
• /
• pp.318-321
• /
• 2007

광역동요법 (photodynamic therapy, PDT)은 악성종양을 치료하기 위해 광과민제와 레이저광을 이용하는 표적지향적 항암치료법이다. 본 연구에서는 PDT에 적용할 수 있는 새로운 광과민제를 스크리닝하기 위해 4종의 phthalocyanine계 광과민제 (silver phthalocyanine, iron (III) phthalocyanine, copper (II) phthalocyanine, nickel (II) phthalocyanine)을 이용하여 폐암과 유방암 세포주에 대한 항암활성 테스트를 수행하였다. 연구결과 phthalocyanine계 광과민제들은 폐암보다 유방암에 높은 활성을 나타냈으며, 그 중 silver phthalocyanine (AgPc)는 정상세포에 대해서는 세포독성이 낮으면서도 물에 용해할 경우 청색증을 유발하지 않아 유방암에 대한 항암제로서의 개발가능성을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권9호
• /
• pp.5371-5374
• /
• 2013

Objective: Photodynamic therapy (PDT) is an emerging therapeutic procedure suitable for the treatment of cervical cancer. However, the side effects of PDT are severe, including skin ulceration, so we designed an experiment to examine the effects of multiple low-dose photodynamic therapy of 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl) porphyrin (Tmpyp4) on tumour growth by utilizing a model in nude mice implanted with Hela cervical cancer cells. Materials and Methods: Female BALB/c nude mice (aged 5-6 weeks, weighing 18-20 g) were used. Hela cervical cancer cells were injected subcutaneously ($1{\times}10^7cells/200{\mu}L$). Ten days after injection, the mice were divided into three groups (n=6), the A group of controls without any treatment, the B group receiving a single-treatment with Tmpyp4 (10 mg/kg, intratumor injection) and irradiation (blue laser, $108J/cm^2$), and the C group given three-treatments with Tmpyp4 (10 mg/kg, intratumor injection) and irradiation at intervals of two days. After starting treatment, tumours were measured every two days, to assess growth. At 2 weeks after the last treatment of C group, tumour tissue and organs were collected from each mouse to evaluate tumor histology and organ damage. Results: Tumour growth in C group was significantly inhibited compared with A and B groups (P<0.05), without any injury to the skin and internal organs. Conclusion: Our novel findings demonstrated that multiple low-dose photodynamic therapy of Tmpyp4 could inhibit cervical cancer growth significantly with no apparent side effects.

• 한국정보통신학회논문지
• /
• 제22권9호
• /
• pp.1237-1242
• /
• 2018

광 역학 치료법은 그 효과가 특정 위치의 암세포에 국한되어있어 주변의 정상 세포에 큰 영향을 미치지 않기 때문에 다양한 부작용이 존재하는 현재의 암 치료법의 대안으로 제안되어 왔다. 이러한 광 역학 치료에는 광감응제를 선택적으로 자극시키기 위해 레이저를 이용하고 있으나, 고가의 치료비용과 높은 발열현상으로 사용에 제한적이다. 그러나 다양한 파장의 빛을 발할 수 있는 소형 발광 다이오드가 저가로 개발됨에 따라 대체가 가능하며, 이는 광 역학 치료 장비의 소형화 및 저가의 치료 장비 개발에 큰 영향을 미치고 있다. 현재 활발하게 연구되는 광 역학 치료법은 다양한 광감응제를 이용하여 암세포의 사멸을 유도하는데 목적이 있으나, 특정한 파장을 중심으로 진행된 연구는 상대적으로 미비한 실정이다. 따라서 본 논문에서는 저렴하며, 발열현상이 작고, 다양한 파장대의 광원지원이 가능한 발광 다이오드를 사용하고 이를 아두이노로 제어하여 새로운 저가의 암세포 증식 억제 모듈을 개발함으로써, 그 효과를 정량적으로 분석하고 새로운 항암치료법을 제시하고자 한다.

• 대한두경부종양학회지
• /
• 제17권1호
• /
• pp.3-7
• /
• 2001

Objectives: New photosensitizer 9-hydroxypheophorbide-$\alpha$(9-HpbD-$\alpha$) was derived from chlorophyll in water with peak absorption at 660nm. 9-HpbD-$\alpha$ was tested with 660 nm diode laser for the anticancer effect of photodynamic therapy. Materials and Methods: Human SNU 1041 cells were seeded into 96 well plate at a density of $$ cells/well for 24 hours. Cells were washed with media containing various concentration of 9-HpbD-$\alpha$ ranging from $0{\mu}g/ml\;to\;3.75{\mu}g/ml$. Then, laser treatment was done with 660nm diode laser ($10mW/cm^2$) at various time setting (0, 30, 60, 90, 120 minutes) and with various time interval (0, 1, 4, 6, 18 hours). The treated cells were incubated 48 hours and MTT assay was done to measure the viability of cells. Results: The viability of cells was more than 90% after laser treatment in control group. The viability of cells was decreased with increasing concentration of 9-HpbD-$\alpha$ and laser treatment time in experimental groups. The viability of cells was decreased significantly as the interval time between addition of 9-HpbD-$\alpha$ and laser irradiation was increased. Conclusion: This study shows the anticancer effect of photodynamic therapy using 9-HpbD-$\alpha$ and 660nm Diode laser on carcinoma cell line. 9-HpbD-$\alpha$ is considerd as one of new photo sensitizers in the field of photodynamic therapy.

• 생체재료학회지
• /
• 제22권4호
• /
• pp.303-310
• /
• 2018

Background: Photodynamic therapy (PDT) is photo-treatment of malignant or benign diseases using photosensitizing agents, light, and oxygen which generates cytotoxic reactive oxygens and induces tumour regressions. Several photodynamic treatments have been extensively studied and the photosensitizers (PS) are key to their biological efficacy, while laser and oxygen allow to appropriate and flexible delivery for treatment of diseases. Introduction: In presence of oxygen and the specific light triggering, PS is activated from its ground state into an excited singlet state, generates reactive oxygen species (ROS) and induces apoptosis of cancer tissues. Those PS can be divided by its specific efficiency of ROS generation, absorption wavelength and chemical structure. Main body: Up to dates, several PS were approved for clinical applications or under clinical trials. $Photofrin^{(R)}$ is the first clinically approved photosensitizer for the treatment of cancer. The second generation of PS, Porfimer sodium ($Photofrin^{(R)}$), Temoporfin ($Foscan^{(R)}$), Motexafin lutetium, Palladium bacteriopheophorbide, $Purlytin^{(R)}$, Verteporfin ($Visudyne{(R)}$), Talaporfin ($Laserphyrin^{(R)}$) are clinically approved or under-clinical trials. Now, third generation of PS, which can dramatically improve cancer-targeting efficiency by chemical modification, nano-delivery system or antibody conjugation, are extensively studied for clinical development. Conclusion: Here, we discuss up-to-date information on FDA-approved photodynamic agents, the clinical benefits of these agents. However, PDT is still dearth for the treatment of diseases in specifically deep tissue cancer. Next generation PS will be addressed in the future for PDT. We also provide clinical unmet need for the design of new photosensitizers.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2022년도 추계학술대회
• /
• pp.323-325
• /
• 2022

본 연구에서는 Chlorin e6 (Ce6)를 입힌 자궁경부암세포를 사멸시키기 위해 레이저 또는 LED를 인가한 PDT (photodynamic therapy)을 실시하고, Ce6 농도와 투여 시간별 사멸도의 변화를 실험하였다. 이를 위해 자궁경부암세포에 광자극을 인가한 후 영상의 처리 및 CCK-8을 이용한 정량적인 평가를 진행하였다. 실험 결과, Ce6은 일정 농도가 높아질수록 많은 양의 세포가 사멸되었고, Ce6를 이용한 PDT 실험군에서 사멸도가 높은 것을 확인하였다. LED와 레이저를 비교하였을 때 레이저는 국소적 위치만 세포의 사멸이 가능하고 LED는 비교적 넓은 범위의 사멸에 효과적인 결과를 확인하였다. 또한 레이저와 같이 국소적 위치를 조사하는 경우 세포 생존도 측정 시 해당 위치의 이미지를 획득한 후 영상처리를 통한 정량분석을 시행하는 방법이 효과적임을 확인하였다.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.515-517
• /
• 2002

Photodynamic therapy (PDT) is a cancer treatment modality which utilizes the cytotoxicity of the active singlet oxygen derived from irradiation of a tumor accumulated photosensitizer. As the oxygen in the singlet state radiates an emission of 1270nm wavelength when it decays to the triplet state, detection of the emission helps us to understand the mechanism of PDT or to evaluate photosensitizers. We detected the 1270nm emission from photosensitizers Photofrin and ATX-SI0 in vitro and in vivo by means of high sensitive NIR detectors. We obtained the maximum amount of singlet oxygen at irradiation wavelength of 665-670nm from a HeLa tumor in a nude mouse which is injected with ATX-S10.

• 대한의생명과학회지
• /
• 제16권4호
• /
• pp.331-339
• /
• 2010

This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogem-mediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem ($10\;{\mu}g/ml$) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem $10\;{\mu}g/ml$ at energy density $15\;J/cm^2$. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.

• International Journal of Oral Biology
• /
• 제41권1호
• /
• pp.39-43
• /
• 2016

Dental caries, the most common oral disease, is a multifactorial disease caused by interactions among bacteria within the dental plaque, food, and saliva, resulting in tooth destruction. Streptococcus mutans has been strongly implicated as the causative organism in dental caries and is frequently isolated from human dental plaque. Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Postantibiotic effect (PAE) is defined as the duration of suppressed bacterial growth following brief exposure to an antibiotic. In this study, the in vitro PAE of PDT using erythrosine and light emitting diode on S. mutans ATCC 25175 was investigated. The PAE of PDT for 1 s irradiation and 3 s irradiation were 1.65 h and 2.1 h, respectively. The present study thus confirmed PAE of PDT using erythrosine on S. mutans.

• Tuberculosis and Respiratory Diseases
• /
• 제78권1호
• /
• pp.36-40
• /
• 2015

Malignant pleural mesothelioma (MPM) is an aggressive, treatment-resistant, and generally fatal disease. A 68-year-old male who was diagnosed with MPM at another hospital came to our hospital with dyspnea. We advised him to take combination chemotherapy but he refused to take the treatment. That was because he had already received chemotherapy with supportive care at another hospital but his condition worsened. Thus, we recommended photodynamic therapy (PDT) to deal with the dyspnea and MPM. After PDT, the dyspnea improved and the patient then decided to take the combination chemotherapy. Our patient received chemotherapy using pemetrexed/cisplatin. Afterwards, he received a single PDT treatment and then later took chemotherapy using gemcitabine/cisplatin. The patient showed a survival time of 27 months, which is longer than median survival time in advanced MPM patients. Further research and clinical trials are needed to demonstrate any synergistic effect between the combination chemotherapy and PDT.