• BMB Reports
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• v.33 no.4
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• pp.353-357
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• 2000

A new assay method of ${\alpha}-Oxidase$ (fatty acid : oxygen dioxygenase, 1-decarboxylating) was developed using a bioluminescence reaction system of marine luminous bacterium, Photobacterium phosphoreum. ${\alpha}$-Oxidase was isolated from a cucumber (Cucumis sativus). Pentadecanoic acid was used as a substrate, and the product, tetradecanal, was analyzed with a bacterial luciferase-coupled reaction. Initial light intensity was directly related to the concentration of tetradecanal in the range of 1 nM to 10 ${\mu}M$. Optimal pH and temperature were 7.5 and $25^{\circ}C$, respectively. Optimal pentadecanoic acid concentration in a standard assay of ${\alpha}$-oxidase was 0.1 mM. The Km value of pentedecanoic acid was $85{\mu}M$. This method is straightforward, rapid, convenient, and easy. Its needs no treatment or extraction of reaction mixture.

• Microbiology and Biotechnology Letters
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• v.27 no.1
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• pp.54-61
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• 1999

Measurement of inhibition of bioluminescence in Photobacterium phosphoreum has been porposed as a sensitive and rapid procedure to monitor toxic substances. However, at first, $EC_{50}$ which shows degree of toxicity to each toxic substances must be calculated. QSAR (Quantitative Structure Activity Relationship) model can be used to estimate $EC_{50}$ to save time and endeavor. Moderately high correlation coefficients ($r^2{\geq}$ 0.97) were calculated from the linear correlation between $EC_{50}$ and molecular connectivity indices of CAHs (chlorinated aliphatic hydrocarbons)such as $^0X$, $^0X^V$, $^1X$, $^2X$ and $^3X^v_c$ and quadratic correlation between $EC_{50}$ and $^0X$, $^0X^V$, $^2X^V$, $^3X_c$, $^3X^V_c$ and P. It shows that the molecular connection indices in carbon structure is contributed to biological characters with linear relation and that in the other one with quadratic relation. The $EC_{50}$ of chlorophenol derivatives had quadratic relation with the value of octanol/water prtition coefficients ($r^2$=0.99) and linear and quadratic relation with the number of chlorine compound (($r^2{\geq}$0.94). This confirms the already known trend of increasing toxicity with increasing ability of a compound to diffuse through cell membrane and number of chlorine substitution.

• Water for future
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• v.27 no.1
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• pp.69-77
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• 1994

As the standard of living improves, the amount of synthetic detergent consumption greatly increases. Detergents which are not treated in the sewer treatment processes, flow into rivers or waterstreams and accelerate the pollution of the surface water resources. Detergents contain lots of toxicants. And it is difficult to evaluate gross toxicity of each toxicant in thereceiving water. In the study, the acute toxicity of the synthetic detergents for home laundering and kitchen use were monitored with daphnia magna and photobacterium phosphoreum. Seven kinds of detergents were tested to evaluate the acute toxicity. The mean 24hr, 48hr-LC50 of the synthetic detergent for home laundering were 4.25%, 2.50% and these for kitchen use were 2.01%, 1.36% respectively. And the mean 5min, 15min-EC50 of the synthetic detergent for home laundering were 1.83%, 1.02%.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.202-206
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• 2000

Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium Phosphoreum were incubated with kanary by-Product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-Product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.11
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• pp.2025-2036
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• 2000

A physicochemical process called ultra rapid coagulation(URC) was applied as a pilot scale to recover eutrophicated lake water at Inkyung Lake located on-campus in Inha university. The URC was uniquely designed to completely remove the presence of phosphorus up to 95%, which in turn leading to lessen the level of eutrophication of lake. For a pilot test of period, the lake was restored showing Carlson's Trophic State Index(TSI) of mesotrophic state which accounts for the reduction of total phosphorus and chlorophyll-a down to approximately 55 from 70 and 73, respectively. A residual presence of aluminum and additional coagulants in the effluent was tested for their potential effects on Photobacterium phosphoreum. The effect concentration ($EC_{50}$-15 min) observed in the effluent revealed that the bioactivity of Photobacterium phosphoreum was not influenced by the residual presence of coagulants. After the pilot test of period, the lake was consequently restored as a mesotrophic state in obtaining the second grade of lake water quality. The URC may be further applied for restoration of greater scale of lake in eutrophication.

• Journal of Microbiology and Biotechnology
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• v.7 no.4
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• pp.250-257
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• 1997

A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.479-479
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• 2000

• Journal of environmental and Sanitary engineering
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• v.10 no.2
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• pp.46-58
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• 1995

Acute toxicity of heavy metals( Mercury, Cadmium, Lead, Chromium and Copper ), Organophosphorus insecticides( EPN, 2, 4- D, and Parathion ), and other chemicals( Arsenic, Cyanide, and Phenol) to Daphnia magna, and Vibrio fischeri was analysed. Daphnia magna to most toxic chemicals was most sensitive among test organisms alld then Daphnia magna seems to be more useful in toxic test of water. Daphnia magna was more sensitive to heavy metals than insecticides and other chemicals. The sensitivity of Visrio JircAeri to heavy metals was not so different from that to insecticides and the sensitivity to other chemicals was low. Visrio JircAeri appears to be more sensitive to toxic chemicals than Photobacterium phosphoreum, which was compared as recorded values of P.phosphoreum. Toxic chemicals were classified as Group 1 which had a high $EC_{50}$ or $LC_{50}$ value and the low increase rate of toxicity according to the increase of concentration, Group 2 which had a high $EC_{50}$ or $LC_{50}$ value and the high increase rate of toxicity, Group 3 which had a low $EC_{50}$ or $LC_{50}$ value and the high increase rate of toxicity, and Group 4 which had a low $EC_{50}$ or $LC_{50}$ value and the low increase rate of toxicity. To Daphnia magna, Lead, Chromium EPN, and Parathion were included in Group 1 : Mercury and Copper in Group 2 : Arsonic and Cadmium in Group 3 : Cyanide, 2, 4-B, and Phenol in Group 4. To Visrio JircAeri, Lead, Chromiurl 2, 4- D, and Parathion were included in Group 1 : Merecury, Cadmium and Arsenic in Group 2 : Cyanide in Group 3 : EPN, Copper, and Phenol in Group 4.

• Microbiology and Biotechnology Letters
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• v.45 no.3
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• pp.250-256
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• 2017

The prevalence of infections due to pathogenic bacteria such as Edwardsiella tarda, Streptococcus parauberis, and Photobacterium phosphoreum in fish farms in Jeju Island and their management by marine-derived biomaterials was studied. In this study, we isolated eight spices type of marine-derived biomaterials from four sea areas of Jeju Island. An antibiotic disc susceptibility test confirmed that the isolated marine-derived biomaterials showed weak resistance only to oxytetracycline and penicillin and sensitivity to the other antibiotics tested, and antimicrobial activity against fish pathogens with the inhibitory zone of 22 mm, 18 mm, and 19 mm for MD-02, MD-04, and MD-06 against E. tarda strains, respectively, and 19 mm, 22 mm, 30 mm, and 29 mm for MD-01, MD-02, MD-04, and MD-06 against S. parauberis strains, respectively, while all the marine-derived biomaterials showed antibacterial activity against P. phosphoreum. Among the eight biomaterials selected, Bacillus subtilis MD-02 displayed the greatest antibacterial activity against the three tested fish pathogens and also displayed susceptibility to antibiotics. The growth of Bacillus subtilis MD-02 was greatest with the carbon source, dextrine; nitrogen source, peptone; and mineral source, $MgSO_4{\cdot}7H_2O$. Hence, the present study confirmed that the isolate B. subtilis MD-02 from Jeju Island could be a potential antimicrobial agent against fish pathogens and a potential pharmacotherapeutic agent.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.101-105
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• 2003

Recombinant bioluminescent bacteria were used to monitor and classify the to xicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Es-Cherichia coli, E, coli GC2 (lac::luxCOABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminestent E. coli, DPD2794 (recA::luxCDABE), a DNA damage Sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classific ation of some azo dyes, In the field, may be possible using these recombinant bioluminescent bacteria.