Browse > Article
http://dx.doi.org/10.4014/mbl.1708.08005

Screening of Antimicrobial Activity of Marine-Derived Biomaterials against Fish Pathogens  

Kim, Dong-Hwi (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University)
Park, So-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University)
Kim, Ji-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University)
Lee, Hae-Ri (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University)
Heo, Moon-Soo (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University)
Publication Information
Microbiology and Biotechnology Letters / v.45, no.3, 2017 , pp. 250-256 More about this Journal
Abstract
The prevalence of infections due to pathogenic bacteria such as Edwardsiella tarda, Streptococcus parauberis, and Photobacterium phosphoreum in fish farms in Jeju Island and their management by marine-derived biomaterials was studied. In this study, we isolated eight spices type of marine-derived biomaterials from four sea areas of Jeju Island. An antibiotic disc susceptibility test confirmed that the isolated marine-derived biomaterials showed weak resistance only to oxytetracycline and penicillin and sensitivity to the other antibiotics tested, and antimicrobial activity against fish pathogens with the inhibitory zone of 22 mm, 18 mm, and 19 mm for MD-02, MD-04, and MD-06 against E. tarda strains, respectively, and 19 mm, 22 mm, 30 mm, and 29 mm for MD-01, MD-02, MD-04, and MD-06 against S. parauberis strains, respectively, while all the marine-derived biomaterials showed antibacterial activity against P. phosphoreum. Among the eight biomaterials selected, Bacillus subtilis MD-02 displayed the greatest antibacterial activity against the three tested fish pathogens and also displayed susceptibility to antibiotics. The growth of Bacillus subtilis MD-02 was greatest with the carbon source, dextrine; nitrogen source, peptone; and mineral source, $MgSO_4{\cdot}7H_2O$. Hence, the present study confirmed that the isolate B. subtilis MD-02 from Jeju Island could be a potential antimicrobial agent against fish pathogens and a potential pharmacotherapeutic agent.
Keywords
Antibiotic substance; antimicrobial activity; fish pathogen; marine derived biomaterials; olive flounder;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Ministry for Food Agriculture Forestry and Fisheries. 2015. Statistical year book of maritime affairs and fisheries.
2 Kim DH, Subramanian D, Jang YH, Heo MS. 2016. Inhibitory effect of transition metal gallium (Ga(NO3)3) on biofilm by fish pathogens. Microbiol. Biotechnol. Lett. 44: 535-539.   DOI
3 Statistics Korea. 2016. Aquaculture Status Survey.
4 Lee CH, Kim PY, Ko CS, Oh DC, Kang BJ. 2007. Biological characteristics of Streptococcus iniae and Streptococcus parauberis isolated from cultured flounder, Paralichthys olivaceus, in Jeju. J. Fish Pathol. 20: 33-40.
5 Nguyen HT, Kanai K. 1999. Selective agars for the isolation of Streptococcus iniae from Japanese flounder, Paralichthys olivaceus, and its cultural environment. J. Appl. Microbiol. 86: 769-776.   DOI
6 Kim DH, Subramanian D, Park SH, Jang YH, Heo MS. 2017. Assessment and potential application of the probiotic strain, Bacillus amyloliquefaciens JFP2, Isolated from fermented seafood- Jeotgal in flounder Paralichthys olivaceus juveniles. Isr. J. Aquac. 69: 1352-1364.
7 Cottrell MT, Moore JA, Kirchman DL. 1999. Chitinases from uncultured marine microorganisms. J. Appl. Environ. Microbiol. 65: 2553-2557.
8 Friedrich AB, Merkert H, Fendert T, Hacker J, Proksch P, Hentschel U. 1999. Microbial diversity in the marine sponge Aplysina cavernicola (formerly Verongia cavernicola) analyzed by fluorescence in situ hybridization (FISH). J. Mar. Biol. 134: 461-470.   DOI
9 Bergman O, Haber M, Mayzel B, Anderson MA, Shpigel M, Hill RT, et al. 2011. Marine-based cultivation of Diacarnus sponges and the bacterial community composition of wild and maricultured sponges and their larvae. Mar. Biotechnol. 13: 1169-1182.   DOI
10 Jung SH, Choi DL, Kim JW, Jo MR, Jee BY, Seo JS. 2009. Pharmacokinetics of oxolinic acid in cultured olive flounder Paralichthys olivaceus by oral administration, injection and dipping. J. Fish Pathol. 22: 125-135.
11 Woo SH, Lee JH, Kim YK, Cho MY, Jung SH, Kim JW, et al. 2010. Effects of garlic Allium sativum extract immersion on the immune responses of olive flounder Paralichthys olivaceus prechallenged with pathogenic bacteria. J. Fish Pathol. 23: 199-209.
12 Chiang WC, Martin M, Jensen PO, Hoiby N, Nielsen TE, Givskov M, et al. 2013. Extracellular DNA shields against aminoglycosides in Pseudomonas aeruginosa biofilms. Antimicrob. Agents Chemother. 57: 2352-2361.   DOI
13 Kim JH, Kim CH, Hacker J, Ziebuhr W, Lee BK, Cho SH. 2008. Molecular characterization of regulatory genes associated with biofilm variation in a Staphylococcus aureus strain. J. Microbiol. Biotechnol. 18: 28-34.
14 Oh EG, Yu HS, Shin SB, Son KT, Park KB, Kwon JY, Lee HJ. 2008. Trimethoprim resistance of Vibrio parahaemolyticus isolated from the fish farm. J. Korean Fish. Soc. 41: 324-329.
15 Lee KW, Park KS. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from seawater. Korean J. Fish Aquat. Sci. 43: 637-641.
16 Park EH, Kim JA, Choi SH, Bin JH, Cheigh HS, Suk DH, et al. 2007. Isolation and antimicrobial susceptibility of Campylobacter jejuni from diarrhea patients. J. Life Sci. 17: 811-815.   DOI
17 Park WM, Kim GH, Hyeon JW. 1995. New synthetic medium for growth of mycelium of Pleurotus species. Korean J. Mycol. 23: 275-283.
18 Sung JM, Choi YS, Shrestha B, Park YJ. 2002. Cultural characteristics of mycelial growth by Cordyceps militaris. Korean J. Mycol. 30: 1-5.   DOI
19 Kang AS, Cha DY, Hong IP, Chang HY, Yu SH. 1994. Studies of cultural condition on the mycelial vegetative growth in Naematoloma sublateritium (Fr.) Karst. Korean J. Mycol. 22: 153-159.
20 Ok M, Choi YS. 2005. Screening of fibrinolytic enzyme producing from microorganisms in korean fermented soybean paste and optimum conditions of enzyme production. Korean J. Food Preserv. 12: 643-649.
21 Oh EG, Son KT, Ha KS, Yoo HD, Yu HS, Shin SB, et al. 2009. Antimicrobial resistance of Vibrio strains from brackish water on the coast of Gyeongsangnamdo. Korean J. Fish Aqua Sci. 42: 335-343.
22 Ryu HS, Shon MY, Cho SJ, Park SK, Lee SW. 2007. Characterization of antibacterial substance-producing Bacillus subtilis isolated from traditional Doenjang. J. Korean Soc. Appl. Biol. Chem. 50: 87-94.
23 Kang B, Zhang X, Wu Z, Wang Z, Park S. 2014. Production of citrinin-free Monascus pigments by submerged culture at low pH. Enzyme Microb. Tec. 55: 50-57.   DOI
24 Chen W, He Y, Zhou Y, Shao Y, Feng Y, Li M, et al. 2015. Edible filamentous fungi from the species Monascus: early traditional fermentations, modern molecular biology, and future genomics. Compr. Rev. Food Sci. Food Saf. 14: 555-567.   DOI