• Journal of Plant Biotechnology
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• 제39권3호
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• 한국작물학회지
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• 제49권6호
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• pp.539-545
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• 2004

M/G RIL 164계통과 그 유전자지도를 이용하여 지역에 따른 벼의 품질과 관련된 양적형질 유전자좌(QTL)를 분석한 결과를 보면 다음과 같다. M/G RIL 164계통의 지역에 따른 단백질함량, 아밀로오스 함량, 지방산함량 및 식미평가치에 있어 빈도분포는 정규분포에 가까운 연속변이를 보였으며, 양친의 범위를 벗어나는 초월분리 현상을 나타내었다. 또한 지역에 따라 품질형질의 분포범위의 폭이 다양하게 나타났으며, 단백질함량은 원주>익산>대구의 순으로, 아밀로오스함량, 지방산함량 및 식미평가치는 대구>익산>원주의 순으로 나타났다. 품질에 관련된 QTLs분석에 있어 단백질함량과 관련하여서는 8개의 QTLs를 확인하였으며, 1번 염색체에서 2개, 3번, 6번, 7번 염색체에서 각각 1개, 8번 염색체에서 3개의 QTLs를 확인할 수 있었으며, 이들 8개의 QTLs가 설명할 수 있는 표현형 변이는 $6.0\~15.2\%$로 나타났다. 아밀로오스함량과 관련하여 6번 염색체에서 1개, 7번 염색체에서 2개의 QTLs를 확인하였다. 3개의 QTLs가 설명할 수 있는 표현형 변이는 $7.3\~24.4\%$로 나타났다. 지방산함량과 관련하여서는 2번과 6번 염색체에서 각각 깨, 3번과 7번 염색체에서 각각 1개의 QTLs를 분석하였으며, 6개의 QTLs로 설명할 수 있는 표현형 변이는 $5.5\~14.0\%$를 보였다. 식미평가치와 관련된 QTLs는 2번과 6번 염색체에서 각각 1개, 7번과 8번 염색체에서 각각 2개의 QTLs가 분석되었으며, 그 6개의 표현형 변이는 $5.5\~10.3\%$로 나타났다.

• 원예과학기술지
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• 제32권3호
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• pp.366-374
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• 2014

이배체 탱자로부터 자연적으로 발생한 동질 사배체 탱자의 수체 형질 관련 표현형 및 유전체 메틸화 변이 정도를 분석하여 후성 유전의 하나인 유전체 메틸화가 동질 사배체의 표현형 변이의 요인으로 작용할 수 있음을 구명하고자 본 실험을 수행하였다. 이배체 탱자에서 유래된 14주의 사배체 탱자로부터 염색체를 분석하여 이수성이 없는 2n = 4X = 36 식물체로 확인하였다. CMA 핵형 분석 결과 염색체가 배가된 동질 사배체임을 확인할 수 있었다. 동질 사배체에서 수고, 수폭, 원가지 수, 원가지 길이, 분지 각도, 마디 길이, 잎의 특성 등 동질 사배체 수체 표현형에 있어서 상당한 변이가 나타남을 확인하였다. 또한 동질 사배체 광합성률에는 큰 차이는 없었지만, SPAD 값에 의한 엽록소 지수에 있어서도 표현형이 다양하게 나타나는 것을 알 수 있었다. 그 외에도 기공 밀도와 공변 세포 길이에 있어서 광범위하게 변이가 관찰되는 것을 알 수 있었다. Global cytosine DNA 메틸화를 분석한 결과 개체 간 메틸화 정도에 차이가 존재함을 알 수 있었다. 동질 사배체 탱자 14주의 절반이 이배체 탱자의 메틸화와 비교하였을 때 2배 이상으로 나타난 것을 확인하였다. 본 연구 결과 동질 사배체에서 나타나는 수체형질 변이가 gene redundency를 줄이기 위한 global cytosine DNA 메틸화와 관계될 수 있음을 확인하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.207-207
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• 2022

Eating and cooking quality (ECQ) is one of the most complex quantitative traits in rice. The understanding of genetic regulation of transcript expression levels attributing to phenotypic variation in ECQ traits is limited. We integrated whole-genome resequencing, transcriptome, and phenotypic variation data from 84 Japonica accessions to build a transcriptome-wide association study (TWAS) based regulatory network. All ECQ traits showed a large phenotypic variation and significant phenotypic correlations among the traits. TWAS analysis identified a total of 285 transcripts significantly associated with six ECQ traits. Genome-wide mapping of ECQ-associated transcripts revealed 66,905 quantitative expression traits (eQTLs), including 21,747 local eQTLs, and 45,158 trans-eQTLs, regulating the expression of 43 genes. The starch synthesis-related genes (SSRGs), starch synthase IV-1 (SSIV-1), starch branching enzyme 1 (SBE1), granule-bound starch synthase 2 (GBSS2), and ADP-glucose pyrophosphorylase small subunit 2a (OsAGPS2a) were found to have eQTLs regulating the expression of ECQ associated transcripts. Further, in co-expression analysis, 130 genes produced at least one network with 22 master regulators. In addition, we developed CRISPR/Cas9-edited glbl mutant lines that confirmed the role of alpha-globulin (glbl) in starch synthesis to validate the co-expression analysis. This study provided novel insights into the genetic regulation of ECQ traits, and transcripts associated with these traits were discovered that could be used in further rice breeding.

• 농업생명과학연구
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• 제46권1호
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• pp.113-121
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• 2012

본 연구는 2005년에서 2009년까지 수집된 150,624개의 국내 젖소 산유능력 검정자료를 사용하여 두 가지 동기우 그룹 정의에 따른 산차, 체세포 점수가 305일로 보정된 유량, 유지방량, 유단백량, 무지고형분량에 미치는 효과와, 유량 및 유성분 간 상관관계에 변화가 나타나는가를 보고자 실시하였다. 첫 번째 동기우 그룹은 동일농장과 출생년도, 계절로 정의하였고, 두 번째는 농장과 분만년도, 계절을 동기우 그룹으로 정의하였다. 동기우 그룹을 출생년도로 구성하였을 때 동기우 그룹과 산차 및 체세포 점수가 유량과 고도의 유의성을 보였고, 유단백질량, 무지고형분량, 유지방량에 대해서도 유의적인 차이를 보였다. 동기우 그룹을 분만년도로 하였을 때에도 유량에 대한 체세포점수 및 산차 효과가 고도의 유의성을 보였고, 유단백질량, 유지방과 무지고형분량에 대해서도 고도의 유의성을 보였다. 출생년도를 사용한 모델에서 생산형질간 표현형상관을 본 결과 유량과 유단백량, 유지방, 무지고형분량은 고도의 정의 상관이 나타났으나, 유량과 체세포 점수 간에는 -0.09의 상관을 나타냈다. 분만년도를 동기우 그룹으로 사용한 모델에서의 표현형상관을 보면 유량과 유단백질 및 유지방간에 출생년도를 사용한 모델과 거의 비슷한 정의 상관을 보였고, 유량과 체세포간에는 -0.10, 유단백질, 유지방, 무지고형분량과 체세포간에는 각각 -0.08, -0.08, -0.11의 상관을 보였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.148-148
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• 2017

Soybean is an important economical resource of protein and oil for human and animals. The genetic basis of seed protein and oil content has been separately characterized in soybean. However, the genetic relationship between seed protein and oil content remains to be elucidated. In this study, we used a combined analysis of phenotypic correlation and linkage mapping to dissect the relationship between seed protein and oil content. A $F_{10:11}$ RIL population containing 222 lines, derived from the cross between two Korean soybean cultivars Seadanbaek as female and Neulchan as male parent, were used in this experiment. Soybean seed analyzed were harvested in three different experimental environments. A genetic linkage map was constructed with 180K SoyaSNP Chip and QTLs of both traits were analyzed using the software QTL IciMapping. QTL analyses for seed protein and oil content were conducted by composite interval mapping across a genome wide genetic map. This study detected four major QTL for oil content located in chromosome 10, 13, 15 and 16 that explained 13.2-19.8% of the phenotypic variation. In addition, 3 major QTL for protein content were detected in chromosome 10, 11 and 16 that explained 40.8~53.2% of the phenotypic variation. A major QTLs was found to be associated with both seed protein and oil content. A major QTL were mapped to soybean chromosomes 16, which were designated qHPO16. These loci have not been previously reported. Our results reveal a signi cant genetic relationship between seed protein and oil fi content traits. The markers linked closely to these major QTLs may be used for selection of soybean varieties with improved seed protein and oil content.

• Asian-Australasian Journal of Animal Sciences
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• 제30권10호
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• pp.1372-1381
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• 2017

Objective: Our study provides information on phenotypes of local chickens and guinea fowl and their body measures as well as on major genes in local chickens in northern Ghana. Methods: Qualitative and morphometric traits were recorded on 788 local chickens and 394 guinea fowl in urban households in Tamale, Ghana. Results: The results showed considerable variation of color traits and numerous major genes in local chickens, while color variations and related genotypes in guinea fowl were limited. In local chickens, white was preferred for plumage, whereas dark colors were preferred for beak and shanks. More than half of the chickens carried at least one major gene, but the contributions of single gene carriers were low. All calculated allele frequencies were significantly lower than their expected Mendelian allele frequencies. We observed higher mean body weight and larger linear body measures in male as compared to female chickens. In female chickens, we detected a small effect of major genes on body weight and chest circumference. In addition, we found some association between feather type and plumage color. In guinea fowl, seven distinct plumage colors were observed, of which pearl grey pied and pearl grey were the most prevalent. Male pearl grey pied guinea fowl were inferior to pearl grey and white guinea fowl in terms of body weight, body length and chest circumference; their shank length was lower than that of pearl grey fowl. Conclusion: Considerable variation in qualitative traits of local chickens may be indicative of genetic diversity within local chicken populations, but major genes were rare. In contrast, phenotypic and genetic diversity in local guinea fowl is limited. Broader genetic diversity studies and evaluation of trait preferences of local poultry producers are required for the design of appropriate breeding programs.

• 한국자원식물학회지
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• 제12권3호
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• pp.198-203
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• 1999

콩 저장 단백질의 대부분은 globulin이며, 이중 7S와 11S가 70% 이상을 차지한다. 따라서 콩 단백질의 조성개량을 위해서는 11S/7S비율 조정이 우선되는데, 본 연구에서는 전기영동(SDS-PAGE)법을 사용하여 콩 단백질 7S와 11S를 분리 확인하고, 이들 분획 단백질의 유전변이를 분석하였다. 국내 3개지역에서 재배된 콩 장려품종 6계통들의 평균 7S 함량은 38.9% 이었고 11S는 61.2%의 함량을 나타내었다. 분산분석 결과 품종간에 는 유의성이 있었지만 지역간에는 변이가 없었으며, 품종 x 지역의 상호작용은 고도의 유의성을 나타내었다. 유전력은 7S분획중의 $\beta$함량이 72.7%로 높게 나타났다. 공분산을 이용한 상관계수 추정에서는 유전상관이 표현형 상관 보다 다소 높게 나타났다. 따라서 7S와 11S의 분획간 함량을 조정함으로써 콩 단백질의 조성을 개량할 수 있으리라 판단된다.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.109-115
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• 1999

A linkage map of Capsicum annuum L. was constructed by random amplified polymorphic DNA (RAPD) markers followed in a backcross population of an intraspecific cross between cultivars HDA210 and Yatsufusa. A total of 420 random primers were tested and 311 polymorphic bands were generated by 158 random primers. Among them, 86 Yatsufusa specific bands generated by 52 primers were examined for mapping. Most bands except three segregated in Mendelian fashion fitting the expected 1:1 ratio. The total length of the map was 533 cM distributed in 15 linkage groups. The map distance between adjacent markers ranged 0 to 32.8 cM, with an average distance of 9.1 cM (63 markers). Some markers were clustered and this may be due to the amplification of a repetitive sequence by the RAPDs. Primer pairs for a sequence characterized amplified region (SCAR) were developed and the segregation scores by the SCAR primers were in accordance with the RAPD data. Two QTL markers for number of axillary shoots and for early flowering were developed. One QTL for early flowering located in the linkage group 3 and explained 61 "io of the phenotypic variation. The other QTL for the number of axillary shoots located in the linkage group 4 explained 55 % of the phenotypic variation.tion.

• Journal of Ginseng Research
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• 제44권6호
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• pp.843-848
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• 2020

This study investigated the inhibitory effect of ginsenoside-Rp1 (G-Rp1) on the ionizing radiation (IR)-induced response in lipopolysaccharide (LPS)-stimulated macrophages and its effects on the malignancy of tumor cells. G-Rp1 inhibited the activation of IR-induced DNA damage-related signaling molecules and thereby interfered with the IR-increased production of nitric oxide (NO) and interleukin (IL)-1β. The inhibitory effect of G-Rp1 increased the survival rate of mice inoculated with CT26 colon cancer cells by suppressing the phenotypic variation of tumor cells induced by conditioned medium obtained from IR- and LPS-treated J774A.1 macrophages.