• 한국식품영양과학회지
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• 제26권3호
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• pp.383-389
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• 1997

Doenjang(fermented soybean paste) was prepared by the series of processes including soaking, cooking, first fermentation(3 days at 30$\pm$2$^{\circ}C$) for the preparation of meju(soybean koji) after inoclulation of Aspergillus oryzae, and further fermentation (60 days at 30$\pm$2$^{\circ}C$) for the ripening after addition of salt 13% to meju. the crude phenolics extracted from defatted soybean and doenjang were fractionated onto the neutral phenolics(isofavonoids) and acidic phenolics(phenolic acids), respectively. Composition and antioxidative characteristics of phenolic fractions were determined. The neutral phenolic fractions contained genistin, genistein and daidzein; on the other hand, acidic phenolic fractions had syringic acid and seven other components. The content of genistin in doenjang dramatically decreased at the early stage of fermentation, whereas the content of genistein rather increased. In addition, the content of syringic acid of acidic phenolic fractions were increased during fermentation. These changes in individual phenolic components affected the antioxidative activity of neutral phenolics or acidic phenolics. antioxidative activity of phenolic compounds were evaluated during soybean fermentation. The antioxidative and free radical scavenging activity of neutral phenolic fractions and acidic phenolic fractions on linoleic acid autoxidation were also investigated.

• Food Science and Biotechnology
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• 제16권3호
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• pp.451-456
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• 2007

The potential antioxidant activities of methanolic extracts from soybean and rice fermented with Monascus sp. were investigated. M. pilosus IFO 480 and M. anka IFO 478 were screened as a suitable strain to promote the antioxidant activities in soybean- and rice- fermentation. The methanol extracts from soybean and rice after fermenting for 20 days at $30^{\circ}C$ resulted in a significant increase in the antioxidant capacities expressed as radical (ABTS and DPPH) scavenging assay and peroxidation inhibition (%) by thiocyanate method and increased (p<0.01) by a 2.6 to 3.1-fold compared with those of the unfermented products. The average antioxidant potentials of Monascus-fermented soybean extracts (MFSE) were significantly (p<0.01) stronger than Monascus-fermented rice extracts (MFRE). A linear correlations between free radical scavenging activity of MFSE and the total phenolics content (r=0.84) and total flavonoids content (r=0.81) were observed. These results indicated that MFSE exhibited stronger (p<0.01) antioxidant activity and contained significantly higher levels (p<0.05) of phenolics than MFRE.

• 한국식품과학회지
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• 제52권4호
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• pp.377-384
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• 2020

Paprika is a popular vegetable with high visual appeal and desirable flavor, the health benefits of which are increasingly attracting interest. In this study, the physical qualities, antioxidant content, and activities of six mini paprika cultivars were investigated. Both the edible part (flesh) and the by-products were studied. The average total phenolics and total antioxidant activities were higher in the flesh than in the by-products. The total flavonoids of the flesh and the byproducts were 16.41 and 37.80 mg/100 g FW, respectively. "YW glory" and "Raon yellow" flesh had the highest (245.52 mg/100 g FW) and lowest (179.96 mg/100 g FW) total phenolics among the six cultivars, respectively. However, the "RD glory" cultivar showed the highest total phenolic content (232.70 mg/100 g FW) among the by-product samples. The total phenolics in the flesh and by-products were highly correlated to the ABTS radical scavenging activity, with R=0.961 and 0.984, respectively.

• 한국식품과학회지
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• 제49권6호
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• pp.714-716
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• 2017

장미꽃을 $30^{\circ}C$, 상대습도 60%의 조건에서 24시간 동안 숙성하여 총페놀화합물 함량, 산화방지 활성, 카테킨 함량을 최대로 증가시키는 조건을 수립하였다. 장미꽃을 차로 이용하기 전에 숙성공정을 통하여 생리활성 물질인 카테킨을 비롯한 페놀화합물의 추출 효율을 증가시킬 수 있고 이에 따라 장미꽃 차의 산화방지효과를 증대 시킬 수 있다.

• 한국작물학회지
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• 제58권3호
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• pp.232-238
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• 2013

새싹재배기에서 7일간 재배된 동부 새싹나물의 부위별 생육, 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항산화효소 활성 차이를 검토하였다. Folin-Denis방법에 따른 총 페놀 함량은 동부나물 자엽의 메탄올 추출물(48.8 mg $kg^{-1}$)이 가장 높았으며, 그 다음이 뿌리(30.8 mg $kg^{-1}$), 하배축(22.2 mg $kg^{-1}$) 순으로 나타났다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 동부나물 자엽의 추출물(82.5%)에서 가장 높았으며, 그 다음이 뿌리(52.6%), 하배축(35.0%) 순으로 나타났다(p < 0.05). 항산화효소 활성은 APX와 CAT 활성은 하배축에서 가장 높았고 POX와 SOD 활성은 뿌리가 가장 높은 활성을 보였다. 이런 경향은 재배 후 5일과 7일째 동부나물에서 같게 나타났다. 따라서 상관분석에 따르면 총 페놀 함량이 총 플라보노이드 함량보다 항산화성과 항산화효소 활성에 더 높은 관련성이 있는 것으로 나타났고 그 생리활성물질 함량과 그 활성 정도는 부위별로 다르게 나타났다.

• 한국양봉학회지
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• 제32권4호
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• pp.327-332
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• 2017

In this study, we studied the changes in antioxidant activity of Actinidia arguta fruit of Autumn Sense cultivar during fruit ripening. The aim of this investigation was to find the knowledge of the changes of physiochemicals associated with fruit quality, antioxidant properties (free-radical scavenging activity and reducing power), total phenolics and vitamin C during fruit ripening. The highest free-radical scavenging activity (at $100{\mu}g/ml$) and reducing power (at $100{\mu}g/ml$) in A. arguta fruit were 78.57% and 0.22, respectively. Total phenolic content and vitamin C content in fruit of 10 days after fruit set were $639.48{\mu}g/g$ and $1052.2{\mu}g/g$, respectively. In general, the antioxidant activity and the related parameters, including total phenolic content and vitamin C content decreased during fruit ripening. These results improve knowledge of the effect of ripening on the antioxidant activity and related compounds contents that could help to establish the optimum A. arguta fruit harvest data for various usages.

• 한국자원식물학회지
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• 제26권2호
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• pp.159-168
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• 2013

대두, 녹두, 및 동부 종자로 7일간 재배된 새싹나물의 생육, 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항산화효소 활성 차이를 검토하였다. 작물별 새싹나물의 총 신장은 녹두와 콩이 동부보다 유의적으로 컸으며 생체중은 오히려 콩과 동부가 녹두보다 유의적으로 높게 나타났다. Folin-Denis방법에 따른 총 페놀 함량은 콩나물의 메탄올 추출물(82.2 mg $kg^{-1}$)이 가장 높았으며, 그 다음이 동부나물(32.2 mg $kg^{-1}$), 녹두나물(24.5 mg $kg^{-1}$) 순으로 나타났다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 전체적으로 낮은 활성이었으나 동부와 녹두 추출물(44와 42%)이 콩나물(25%)보다 비교적 높은 활성을 보였다. 항산화효소 활성은 APX와 POX활성은 동부가 가장 높았고 그 다음이 녹두, 콩 순으로 나타났고, CAT와 SOD 활성은 콩나물이 동부와 녹두나물보다 높게 나타났다. 따라서 본 연구는 총 페놀 함량($r^2$ = 0.53 ~ 0.90)과 총 플라보노이드 함량($r^2$ = 0.47 ~ 0.94)은 항산화성과 항산화효소 활성에 높은 연관성이 있으며, 그 함량과 활성은 작물별로 다르게 나타남을 확인하였다.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.370-375
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• 2011

The antioxidative activity and the polyphenolic composition were examined in four different cultivars of apple (Malus domestica), 'Fuji', 'Tsugaru', 'Hongro' and 'Kogetsu', and their parts (peel, core, pulp and juice). The total phenolics, flavonoids and anthocyanins differed among the tested cultivars and parts. Peel parts had the highest total phenolics and anthocyanin content. Contributions of those phenolics to total antioxidative activity were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, and the linoleic acid oxidation assay. Concentration of phenolics contributes significantly to the total antioxidative activity of apples. Clearly, apple peels, especially from Hongros and Kogetsus, possess high levels of phenolic compounds and antioxidants. Therefore, apple peels may potentially function as a value-added ingredient.

• 대한예방한의학회지
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• 제13권1호
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• pp.59-67
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• 2009

Objectives : This research aimed to evaluate the effects of Rubus coreanus $M_{IQ}$. Methods: These extracts from fresh R. coreanus $M_{IQ}$. were investigated with radical scavenging activity, the content of total phenolics, ferric thiocyanate method and flavonoid contents Results: These results revealed that the ethyl acetate extract from fresh R. coreanus $M_{IQ}$. possesses remarkable radical scavenging activity. The content of total phenolics of the ethanol extract from dried R. coreanus $M_{IQ}$. ($IC_{50}$=155.87${\pm}$8.8${\mu}$g/mL) is the highest among all of the samples. The stronger radical-scavenging activity ($IC_{50}$=77.72${\pm}$2.1${\mu}$g/mL) of ethyl acetate extract from dried R. coreanus $M_{IQ}$. should be related to its phenolic content as measured by gallic acid test. Conclusion: These results suggest that dried R. coreanus $M_{IQ}$. may act as a potential antioxidant agent.

• 한국식품과학회지
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• 제26권3호
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• pp.310-316
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• 1994

한국인의 식생활에서 널리 이용되고 있는 식물성 식품 및 민간요법에서 이용되는 비상용식품 45가지를 선택하여 총페놀함량은 Folin-Denis 법으로, 축합형 탄닌의 함량은 vanillin 법과 modified vanillin 법으로, 단백질 침전성 페놀물질의 함량은 단백질 침전법으로 측정하였다. 총페놀 함량(건물기준)은 $0.1{\sim}5.8%$로 감잎, 밤속껍질, 모과, 호두, 해바라기씨, 칡뿌리에서는 2% 이상의 높은 함량을 보였다. 축합형 탄닌함량(건물기준)은 $0{\sim}48%$로 모과 밤속껍질에서 매우 높이 나타났다. 단백질 침전성 페놀성분의 함량(건물 기준)은 $0.4{\sim}2.2%$로 밤속껍질, 호두, 모과에서 매우 높았고 그들의 단백질 침전능은 트립신보다 펩신이나 알부민에서 더 잘 나타났다. 식품중 페놀성물질 함량간의 상관관계를 보면 총페놀 함량과 단백질 침전성 페놀물질 함량(r=0.65)과 축합형 탄닌함량(r=0.56)간에 높은 상관관계를 보였으나, 축합형 탄닌함량과 단백질 침전성 페놀물질 함량간에는 비교적 낮은 상관관계(r=0.39)를 보였다. 축합형 탄닌의 중합도를 예측하는 vanillin/Folin-Denis 비를 살펴본 결과 모과, 밤속껍질, 수수에서 높은 값을 보여 결국 낮은 중합도를 보였다. 메밀, 도토리, 쑥, 칡 등을 이용한 가공식품에서의 페놀물질 함량은 원료상태에서 보다 낮은 함량을 보여 식품단백질의 이용율에는 큰 장애를 주지 않을 것으로 판단되었다.