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http://dx.doi.org/10.7732/kjpr.2013.26.2.159

Phenolics Content and Antioxidant Activity of Sprouts in Several Legume Crops  

Chon, Sang-Uk (EFARINET Co. Ltd., BI Center, Chosun University)
Kim, Dong-Kwan (Jeonnam Agricultural Research and Extension Services)
Kim, Young-Min (Dongeuinara Co. Ltd., Biotechnology Industrialization Center, Dongshin University)
Publication Information
Korean Journal of Plant Resources / v.26, no.2, 2013 , pp. 159-168 More about this Journal
Abstract
The study was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme activity for the extract from 7 days old sprouts of cowpea (cv. "Seowon"), mungbean (cv. "Owool") and soybean (cv. "Pungsannamulkong"). Sprout length and weight of soybean sprouts were higher than those of cowpea and mungbean sprouts. Total phenolics content [mg ferulic acid equivalents (FAE) $kg^{-1}$ DW] was highest in soybean sprout extracts (82.2 mg $kg^{-1}$), followed by cowpea (32.2 mg $kg^{-1}$) and mungbean (24.5 mg $kg^{-1}$) sprout extracts (p < 0.05). The result of total flavonoid level [mg rutin equivalents $kg^{-1}$ DW] had same tendency to the total phenolics, showing lower amounts. The antioxidant activity of the methanol extracts from all the plant dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in cowpea (44%) and mungbean (42%) sprouts than in soybean sprouts (25%). Among antioxidant enzymes, APX and POX activities were highest in cowpea sprouts and CAT and SOD activities in soybean sprouts. The results showed that total phenolics content ($r^2$ = 0.5320 ~ 0.9032) and total flavonoids level ($r^2$ = 0.4672 ~ 0.9380) were highly correlated with antioxidant or with antioxidant enzyme activity, and that the level and activity of biologically active substances were different depending on plant species.
Keywords
Antioxidant enzyme activity; Cowpea; DPPH radical scavenging activity; Mungbean; Soybean; Sprouts; Total flavonoids; Total phenolics;
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1 Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22:867‐880.
2 Park, I.K. and S.D. Kim. 2003. Sugar and free amino acid content of chitosan‐treated soybean sprouts. J. Chitin Chitosan 8:105‐110.
3 Salunke, B.K., H.M. Kotkar, P.S. Mendki, S.M. Upasani and V.L. Maheshwari. 2005. Efficacy of flavonoids in controlling Callosobruchus chinensis (L.) (Coleoptera: Bruchidae), a post‐harvest pest of grain legumes. Crop Prot. 24:888-893.   DOI   ScienceOn
4 SAS (Statistical Analysis Systems) Institute. 2000. SAS/STAT user's guide. Version 7. Electronic Version. Cary, NC, USA.
5 Siddhuraju, P. and K. Becker. 2007. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chemistry 101:10-19.   DOI   ScienceOn
6 Singleton, V.L. and J.A. Rossi. 1965. A colorimetry of total phenolics with phosphomolybdic‐phosphotungstic acid reagents. American J. Enol. Viticult. 16:144‐158.
7 Sowmya, P. and P. Rajyalakshmi. 1999. Hypocholesterolemic effect of germinated fenugreek seeds in human subjects. Plant Foods Hum. Nutr. 53:359-365.   DOI   ScienceOn
8 박혜원. 1995. 녹두이용 음식의 유래와 그 조리 과학성. 국민영양 95(4):40.
9 배효원, 유태종. 1967. 대두 발아중의 각 기관 단백질 및 자엽 RNA 변동에 관한 연구. 한국농화학회지 8:81‐86.
10 신효선. 1974. 대두 발아 중 지질대사에 관한 연구. 제1보 조지방량 및 지질성분의 변화에 관하여. 한국농화학회지 17(4):240‐245.
11 이성우. 1957. 숙주의 영양생장과 한국적 조리에 의한 비타민 C의 소장에 관한 연구. 대한가정의학회지 3:357.
12 조재영. 1990. 사정 전작. 향문사, 서울, 한국. p. 535.
13 Egley, G.H., R.N. Paul, K.C. Vaughn and S.O. Duke. 1983. Role of peroxidase in the development of waterimpermeable seed coats in Sida spinosa L. Plant 157:224‐232.   DOI   ScienceOn
14 Frota, K.M.G., S. Mendonca, P.H.N. Saldiva, R.J. Cruz and J.A.D. Ageas. 2008. Cholesterol‐lowering properties of whole cowpea seed and protein isolate in hamsters. J. Food Sci. 73:235‐240.
15 Gray, J.I. and L.R. Jr. Dugan. 1975. Inhibition of N‐nitrosamine formation in model food systems. J. Food Sci. 40:981‐984.   DOI
16 Gutierrez‐Uribe, J.A., I. Romo‐Lopez and S.O. Serna‐Saldívar. 2011. Phenolic composition and mammary cancer cell inhibition of extracts of whole cowpeas (Vigna unguiculata) and its anatomical parts. J. Funct. Foods 3:290‐297.   DOI   ScienceOn
17 Hofsten, B. 1979. Legume sprout as a source of protein and other nutrients. J. Am. Oil Chemists' Soc. 56:382‐392.   DOI
18 Jeong, S.J., T.H. Kang, E.B. Ko and Y.C. Kim. 1998. Flavonoids from the seeds of Phaseolus radiatus. Kor. J. Pharmacogn. 29(4):357‐259 (in Korean).   과학기술학회마을
19 Kang, S.J., J.Y. Oh and J.D. Jung. 1999. Changes of antioxidant enzyme activities in leaves of lettuce exposed to ozone. J. Kor. Soc. Hort. Sci. 40:541‐544(in Korean).
20 Kim, B.J., J.H. Kim, Y. Hea and H.P. Kim. 1998. Antioxidant and anti‐inflammatory activities of the mungbean. Cos. Toilet. Mag. 113:71‐74.
21 Kim, D.K., S.C. Jeong, S. Gorinstein and S.U. Chon. 2012. Total polyphenols, antioxidant and antiproliferative activities of different extracts in mungbean seeds and sprouts. Plant Foods Hum. Nutr. 67:71-75.   DOI   ScienceOn
22 Kim, E.H., S.H. Kim, J.I. Chung, H.Y. Chi, J.A. Kim and I.M. Chung. 2006. Analysis of phenolic compounds and isoflavones in soybean seeds (Glycine max (L.) Merill) and sprouts grown under different conditions. Eur. Food Res. Technol. 222:201-208.   DOI   ScienceOn
23 Kim, J.G., S.K. Kim and J.S. Lee. 1988. Fatty acid composition and electrophoretic patterns of protein of Korean soybeans. Korean J. Food Sci. Technol. 20:263‐271 (in Korean).
24 Kim, S.D., S.H. Kim and E.H. Hong. 1993. Composition of soybean sprout and its nutritional value. J. Korean Soybean Res. 1:1‐9 (in Korean).
25 Krygier, K., F. Sosulski and H. Lawrence. 1982. Free, esterified and insoluble‐bound phenolic acids. 1. Extraction and purification procedure. J. Agric. Food Chem. 30:330‐334.   DOI
26 Lister, C.E., J.E. Lancaster, K.H. Sutton and J.R.L. Walker. 1994. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. J. Sci. Food and Agric. 64:155‐161.   DOI   ScienceOn
27 Madhujith, T., M. Naczk and F. Shahidi. 2004. Antioxidant activity of common beans (Phaseolus vulgaris L.). J. Food Lipids 11:220-233.   DOI   ScienceOn
28 Mishra, N.P., R.K. Mishra and G.S. Singhal. 1993. Changes in the activities of anti‐oxidant enzymes during exposure of intact what leaves to strong visible light at different temperatures in the presence of protein synthesis inhibitors. Plant Physiol. 102:903‐910.   DOI
29 Bau, H.M., C. Villaume, J.P. Nicolas and L. Mejean. 1997. Effect of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean (Glycine max) seeds. J. Sci Food Agric. 73(1):1-9.   DOI
30 Banwart, W.L., P.M. Porter, T.C. Granato and J.J. Hassett. 1985. HPLC separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. J. Chem. Ecol. 11:383‐395.   DOI   ScienceOn
31 Beyer, W.F. and I. Fridovich. 1987. Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Anal. Biochem. 161:559‐566.   DOI   ScienceOn
32 Blois, M.S. 1958. Antioxidant determinations by use of a stable free radical. Nature 26:1199‐1200.
33 Blume, E. and J.W. McClure. 1980. Developmental effects of Sandoz 6706 on activities of enzymes of phenolic and general metabolism in barley shoots grown in the dark or under low or high intensity light. Plant Physiol. 65:238‐244.   DOI   ScienceOn
34 Bowler, C., M. Van Montagu and D. Inze. 1992. Superoxide dismutases and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:83‐116.   DOI   ScienceOn
35 Bradford, M.M. 1976. "Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding", Anal. Biochem. 72:248‐254.   DOI   ScienceOn
36 Branca, F., and S. Lorenzetti. 2005. Health effects of phytoestrogen. Forum Nutr. 57:100‐111.
37 Cai, R., N.S. Hettiarachchy and M. Jalaluddin. 2003. Highperformance liquid chromatography determination of phenolic constituents in 17 varieties of cowpeas. J. Agr. Food Chem. 51:1623‐1627.   DOI   ScienceOn
38 Chen, G.X. and K. Asada. 1989. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 30:987‐998.
39 Chung, I.M., K.H. Kim, D.K. Song and B.H. Kang. 1999. Physiological responses of rice (Oryza sativa L.) varieties to ozone. Kor. J. Environ. Agr. 18:11‐17 (in Korean).   과학기술학회마을
40 Chen, L.H., C.E. Well and J.R. Fordham. 1975. Carbohydrate analysis : A practical approach. IRL press Ltd., Oxford, 국가명. p. 23.
41 Danisova, C., E. Holotnakova, B. Hozovaand V. Buchtova. 1994. Effect of germination on a range of nutrients of selected grain and legumes. Acta Alimentaria 23:287-298.
42 Davies, K.J.A. 1995. Oxidative stress: The paradox of aerobic life, p. 1‐32. In C. Rice‐Evans, Halliwell B. and G.G. Lunt (eds.), Free radicals and oxidative stress: Environment, drugs, and food additives. Biochem. Soc. Symp. 61, Portlant Press, London, UK.
43 Abdullah, A. and R.E. Baldwin. 1984. Mineral and vitamin contents of seeds and sprouts of newly available smallseeded soybeans and market samples of mungbeans. J. Food Sci. 49:656-657.   DOI
44 Anderson, M.D., T.K. Prasad and C.R. Stewart. 1995. Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiol. 109:1247‐1257.   DOI