KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Difference in Growth, Phenolics Content and Antioxidant Activity of Cowpea Sprouts at Different Plant Parts

동부나물의 부위별 생육, 폴리페놀 및 항산화성 차이

  • Received : 2013.01.17
  • Accepted : 2013.07.22
  • Published : 2013.09.30
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Abstract

An experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme status for the extracts from 5 and 7-day old sprouts (DOS) of cowpea (Vigna unguiculata L. Walp). Total phenolics [mg ferulic acid equivalents (FAE) $kg^{-1}$ DW] content was highest in cotyledon extracts (48.8 mg $kg^{-1}$), followed by roots (30.8 mg $kg^{-1}$) and hypocotyl (22.2 mg $kg^{-1}$) extracts (p < 0.05) from 5 DOS. The result of total flavonoid level [mg rutin equivalents $kg^{-1}$ DW] had same tendency to the results of total phenolics, showing lower amount ranges. The antioxidant activity of the methanol extracts from all the plant dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in cotyledon extracts (82.5%) than in root (52.6%) or hypocotyl parts (35.0%) from 5 DOS. Among antioxidant enzymes, APX and CAT activities were highest in cotyledon part and POX and SOD activities in root part of 5 and 7 DOS. The results showed that total phenolics content ($r^2$ = 0.1516~0.9911) were more highly correlated with antioxidant activity than total flavonoids level ($r^2$ = 0.0113~0.9442), and that the level and activity of physiological-active substances were different depending on plant part of the sprout.

새싹재배기에서 7일간 재배된 동부 새싹나물의 부위별 생육, 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항산화효소 활성 차이를 검토하였다. Folin-Denis방법에 따른 총 페놀 함량은 동부나물 자엽의 메탄올 추출물(48.8 mg $kg^{-1}$)이 가장 높았으며, 그 다음이 뿌리(30.8 mg $kg^{-1}$), 하배축(22.2 mg $kg^{-1}$) 순으로 나타났다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 동부나물 자엽의 추출물(82.5%)에서 가장 높았으며, 그 다음이 뿌리(52.6%), 하배축(35.0%) 순으로 나타났다(p < 0.05). 항산화효소 활성은 APX와 CAT 활성은 하배축에서 가장 높았고 POX와 SOD 활성은 뿌리가 가장 높은 활성을 보였다. 이런 경향은 재배 후 5일과 7일째 동부나물에서 같게 나타났다. 따라서 상관분석에 따르면 총 페놀 함량이 총 플라보노이드 함량보다 항산화성과 항산화효소 활성에 더 높은 관련성이 있는 것으로 나타났고 그 생리활성물질 함량과 그 활성 정도는 부위별로 다르게 나타났다.

Keywords

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