• Title/Summary/Keyword: phenolic-OH

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Identification of Biologically Active Substances from Medicinal Plants (약용식물(藥用植物)(음나무, 오가피)로부터 생리활성물질(生理活性物質) 검정(檢定))

  • Lee, I.J.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.289-298
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    • 1987
  • This study was conducted to investigate the presence of biologically active substances such as phenolic acids, fatty acids and organic acids in the medical plants like Kalopanax pictum and Acanthopanacis cortex. Alcohol extracts of K. pictum and A. cortex showed complete inhibition of lettuce seed germination, indicating that these plants contained the biologically active substances. Eleven phenolic acids including protocatechuic acid were identified from K. pictum and A. cortex by GLC, and the contents of total phenolic acid were 1.7917mg/g in K. pictum, and 0.9567mg/g in A. cortex. Polyphenols such as neochlorogenic acid, chi orogenic acid, scopoletin, rutin and kaempferolglycoside which were not detected by GLC were anayzed by HPLC, and among phenolic acids indentified chi orogenic acid seemed to be the major acid in both K. pictum and A. cortex presented in amount of 23.7 and 13.0ppm, respectively. K. pictum contained 5.26mg/g of fatty acids and 27.69mg/g of organic acids, and A. cortex possessed 3.22mg/g of fatty acids and 9.80mg/g of organic acids, linoleic and oxalic acid appeared to be the major fatty and organic acids, representing more than 50% of total fatty acids and 80% of total organic acids.

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Agricultural Characters, Phenolic and Nutritional Contents, and Antioxidant Activities of Pigeon Pea (Cajanus cajan) Germplasms Cultivated in the Republic of Korea

  • Choi, Yu-Mi;Hyun, Do Yoon;Lee, Sukyeung;Yoon, Hyemyeong;Lee, Myung-Chul;Oh, Sejong;Ko, Ho-Cheol;Shin, Myoung-Jae;Hur, Onsook;Yi, Jung Yoon;Desta, Kebede Taye
    • Korean Journal of Plant Resources
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    • v.33 no.1
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    • pp.50-61
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    • 2020
  • Pigeon pea (Cajanus cajan) is an important legume species that produces seeds that are rich in phenolic compounds and dietary nutrients. The aim of the present study was to evaluate the agricultural characters, phenolic and nutritional contents, and antioxidant capacities of seeds from 82 pigeon pea germplasms cultivated in the Republic of Korea. The accessions exhibited compact (24.39%), semi-spreading (74.39%), or spreading (1.22%) growth habits and determinate (89.02%), indeterminate (8.54%), or semi-determinate (2.44%) flowering patterns. Days to 75% maturity ranged from 30 to 72 d, and yield per plant ranged from 6.00 to 148.60 g. Meanwhile, total phenolic, crude protein, crude fiber, and dietary fiber contents ranged from 16.42 ± 0.62 to 29.67 ± 0.43 mg gallic acid equivalent per g of dried extract, from 16.76 ± 6.74% to 22.61 ± 0.05%, from 4.70 ± 0.24 to 8.63 ± 0.02%, and from 12.98 ± 0.71 to 33.19 ± 1.50%, respectively. In addition, DPPH radical-scavenging capacity ranged from 1.61 ± 0.10 to 16.04 ± 2.30 mg ascorbic acid equivalent per g of dried extract, and Trolox equivalent antioxidant capacity ranged from 3.03 ± 0.86 to 42.24 ± 0.72 mg Trolox equivalent per g of dried extract. Phenolic content was correlated with both DPPH radical-scavenging capacity (r = 0.63) and Trolox equivalent antioxidant capacity (r = 0.29). Nine accessions (IT170290, IT170291, IT170270, IT170276, IT170379, IT170386, IT170388, IT170418, and IT170340) exhibited early maturity, compact and erect growth habits, and above average antioxidant activities and phenolic and protein contents. In addition, accessions IT170290 and IT170291 were especially promising pigeon pea germplasms to grow, owing to various favorable characteristics (e.g., high yield and dietary fiber content). Hence, these accessions could be useful cultivars to the Republic of Korea if considered in future agricultural systems.

Antioxidative Effectiveness of Water Extract and Ether Extract in Wormwood(Artemisia montana Pampan) (쑥(산쑥)의 물 추출물과 에테르 추출물의 항산화 효과)

  • Lee, Gee-Dong;Kim, Jeong-Sook;Bae, Jae-Oh;Yoon, Hyung-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.17-22
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    • 1992
  • Water extract, and free-, ester- and insoluble phenolic acids were extracted from wormwoods. The extracts were then dissolved in equal amounts of an edible soybean oil, and the resulting substrates, portion of the soybean oil (control), 0.02% BHA and 0.02% BHT were stored in incubator at $45^{\circ}C{\pm}1.0^{\circ}C$ for 32days. Peroxide values (POV) and thiobarbituric acid (TBA) values of control and the substrates were determined during th9 storage period. The POV of control, BHA, BHT and six substrates which were contained water extracts 0.5%, 1% and 1.5%, and free-, ester- and insoluble phenolic acids after 24days of storage were 77, 43, 17, 38, 26, 34, 45, 28 and 16m mole/kg oil respectively, The TBA values after 32days of storage were 0.278, 0.170, 0.070, 0.200, 0.178, 0.235, 0.167, 0.136, 0.062, respectively, It was found that the antioxidant activity in water extracts was highest in water 1 (the substrates containing water extracts 1%). It was also found that antioxidant activities in the phenolic acids were decreased in the following order ; insoluble phenolic acids>ester form>free phenolic acids. The phenolic acids separated and tentatively identified by Gas chromatography were catechol, vanillin, umbelliferone, p-coumaric acid, ferulic acid caffeic acid, protocatechuic acid, p-hydroxy benzoic acid and syringic acid.

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Glucuronidation of Hydroxylated Polychlorinated Biphenyl by Channel Catfish Liver (챠넬메기 간에서 Hydroxylated Polychlorinated Biphenyl의 Glucuronidation)

  • Shin, Hea-Soon
    • Environmental Analysis Health and Toxicology
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    • v.23 no.3
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    • pp.195-200
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    • 2008
  • Hydroxylated polychlorinated biphenyl (OH-PCBs)는 PCB의 CYP-dependent oxidation의 대사물로서 잠재적 독성이 강하고 지질친화성을 가지며 생물군에 지속적인 축적성을 나타낸다. OH-PCBs의 해독화 효능을 조사하기 위하여 channel catfish 간에서 glucuronidation을 통한 해독작용 가능성을 평가하고 biphenyl 구조에 다양한 염소치환의 구조적 차이점에 따른 영향을 비교 분석하여 보았다. Kinetic parameters에서 $K_m$$V_{max}$$192{\sim}871{\mu}M$, $869{\sim}1774$ pmo1/min/mg으로써 4'-OH-PCB35와 4'-OH-PCB69이 가장 높은 속도의 glucuronidation을 나타냈으며, 구조적 차이점에서 phenolic group에 한 개의 염소치환이 존재할 경우보다 두 개의 염소치환이 존재할 경우에 OH-PCBs(p<0.001)의 glucuronidation에 대한 $V_{max}$를 현저하게 낮추는 결과를 보였다.

Antioxidant and Anticancer Activities of Extract from Artemisia capillaries (인진쑥 추출물의 항산화 및 항암 활성)

  • Jung, Mee-Jung;Yin, Yu;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Korean Journal of Pharmacognosy
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    • v.39 no.3
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    • pp.194-198
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    • 2008
  • Artemisia capillaries is a major important food and medicinal resource in Korea. In order to confirm the biological activities of Artemisia capillaries, we investigated antioxidant and anticancer activities from in vitro assays. The Artemisia capillaries methanol (MeOH) extracts was used for the evaluation of DPPH scavenging, total phenolic content, total flavonoid content, hydroxyl radical (${\bullet}OH$) scavenging, reducing power assay as antioxidant activity, as well as anticancer activities as MTT assay. As a result, the Artemisia capillaries MeOH extracts showed potent antioxidative activity and anticancer activity in vitro. These results suggest that the Artemisia capillaries MeOH extracts have a potential alleviated oxidation process, cell motility activity, and tumorigenesis.

Functional food activities of extracts from Pinus densiflora root (동송근(Pinus densiflora root) 추출물의 기능성식품 활성)

  • Lee, Eun-Ho;Kim, Na-Hyun;Park, Mi-Jung;Hong, Eun-Jin;Park, Ki-Tae;An, Bong-Jeun;Ahn, Dong-Hyun;Cho, Young-Je
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.110-116
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    • 2016
  • In this study, the antioxidative activity and functional food activities of water and ethanol extracts from Pinus densiflora root were examined. It was more effective to use ethanol than water when extracting phenolic compounds. The extracted phenolic compounds from Pinus densiflora root for biological activities were examined. The phenolic compounds extracted with water and 80% EtOH were $1.86{\pm}0.04mg/g$ and $6.85{\pm}0.16mg/g$, respectively. DPPH free radical scavenging activity of water and EtOH were each 86% and 85% at $100{\mu}g/mL$ phenolics, respectively. ABTS radical decolorization activity was 48% in water and 68% in EtOH at $200{\mu}g/mL$. Antioxidant Protection Factor (PF) were 1.74 PF in water and 1.96 PF in EtOH at $50{\mu}g/mL$. TBARs of water and EtOH were 93% and 98%, respectively at $100{\mu}g/mL$. The inhibition activity on xanthine oxidase was 83.7% in water extracts and 79.6% in ethanol extracts. Inhibition on xanthine oxidase of water and ethanol extracts showed a higher inhibition effect than allopurinol. The inhibition activity on ${\alpha}$-glucosidase was 14.8% in water extracts and 91.6% in ethanol extracts. The result suggests that P. densiflora root extracts may be useful as as functional food material.

Synthesis of an Environmentally Friendly Phenol-Free Resin for Printing Ink

  • Ha, Young-Baeck;Jin, Ming Yu;Oh, Sung-Sang;Ryu, Do-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.33 no.10
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    • pp.3413-3416
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    • 2012
  • Phenol-free resin was synthesized and its printing ink properties were investigated. The phenol-free resin was produced by esterification of poly phthalate and Diels-Alder adduct of rosin anhydride. Compared to rosin modified phenolic resin, eco-friendly phenol-free resin showed better vehicle properties in terms of gloss, yellowing, runability, and storage stability. The results suggest the utility of phenol-free resin instead of conventional rosin modified phenolic resin.

Three Phenolic Glycosides from Gastrodia elata

  • Chae, Sung-Wook;Lee, A-Yeong;Lee, Hye-Won;Yoon, Tae-Sook;Moon, Byeong-Cheol;Choo, Byung-Kil;Kim, Ho-Kyoung
    • Journal of Applied Biological Chemistry
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    • v.51 no.2
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    • pp.61-65
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    • 2008
  • Three phenolic glycosides, gastrodin (1), parishin (2), and parishin B (3) were isolated from the n-BuOH fraction of Gastrodia elata (Orchidaseae) by medium pressure liquid chromatography. Their chemical structures were identified by the interpretation of their spectral data and direct comparison with literature values. Gastrodin and parishin were isolated as the major constituents in G. elata. Gastrodin may be a suitable index component for quality control of G. elata.

SEPARATION, IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM ALFALFA PLANT (알팔파의 생리활성물질 분리 및 동정)

  • Chung, Ill-Min;Kim, Ki-June
    • Analytical Science and Technology
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    • v.7 no.3
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    • pp.403-411
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    • 1994
  • To isolate, purity and identify of bioactive compounds involved in alfalfa allelopathy and/or autotoxicity, experiment was conducted. Isolation and separation procedures used from an 80% methanol extract of fresh alfalfa leaves(1kg), silica gel thin layer chromatography(TLC), followed by Droplet Counter Current Chromatography(DCCC). Preliminary identification was examined by high preformance lipid chromatography(HPLC). Four phenolic compound, salicylic acid, scopoletin, rutin, and quercetin, were identified and identified all compounds were phytotoxic to alfalfa seed germination and seedling growth. Among these compounds, quercetin treatment($10^{-3}M$) was most inhibitory to alfalfa seed germination and seedling growth. These compounds may be, at least in part, involved autotoxicity and allelopathy.

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Cerebrosides and Phenolic Constituents of Prunus padus L. (귀룽나무의 쎄레브로사이드 및 페놀성 성분)

  • Na, Dae-Su;Yang, Min-Cheol;Lee, Kyu-Ha;Lee, Kang-Ro
    • Korean Journal of Pharmacognosy
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    • v.37 no.3
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    • pp.125-129
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    • 2006
  • The chromatographic separation of n-BuOH extract of the aerial parts of Prunus padus (Rosaceae) led to the isolation of two cerebrosides, and six phenolic compounds. Their structure were identified to be pinelloside (1), soyacebroside I (2), $quercetin-3-O-{\beta}-D-galactopyranoside$ (3), nudiposide (4), (+)-isolarisiresinol $9'-O-{\beta}-D-xylopyroanoside$ (5), khaephuoside A (6) and icariside F2(7) by physicochemical and spectroscopic methods. The compounds $1,5{\sim}7$ are first isolated from the genus Prunus.