• Title/Summary/Keyword: phenolic acids

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Identification of Biologically Active Substances from Medicinal Plants (약용식물(藥用植物)(음나무, 오가피)로부터 생리활성물질(生理活性物質) 검정(檢定))

  • Lee, I.J.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.289-298
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    • 1987
  • This study was conducted to investigate the presence of biologically active substances such as phenolic acids, fatty acids and organic acids in the medical plants like Kalopanax pictum and Acanthopanacis cortex. Alcohol extracts of K. pictum and A. cortex showed complete inhibition of lettuce seed germination, indicating that these plants contained the biologically active substances. Eleven phenolic acids including protocatechuic acid were identified from K. pictum and A. cortex by GLC, and the contents of total phenolic acid were 1.7917mg/g in K. pictum, and 0.9567mg/g in A. cortex. Polyphenols such as neochlorogenic acid, chi orogenic acid, scopoletin, rutin and kaempferolglycoside which were not detected by GLC were anayzed by HPLC, and among phenolic acids indentified chi orogenic acid seemed to be the major acid in both K. pictum and A. cortex presented in amount of 23.7 and 13.0ppm, respectively. K. pictum contained 5.26mg/g of fatty acids and 27.69mg/g of organic acids, and A. cortex possessed 3.22mg/g of fatty acids and 9.80mg/g of organic acids, linoleic and oxalic acid appeared to be the major fatty and organic acids, representing more than 50% of total fatty acids and 80% of total organic acids.

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Determination of Phenolic acids and Flavonol Aglycone Contents in Orostachys japonicus A. Berger Grown under Various Cultivation Conditions

  • Jang, Sang-Hun;Lee, Sang-Gyeong;Kang, Jin-Ho;Park, Jong-Cheol;Shin, Sung-Chul
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.5
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    • pp.311-316
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    • 2006
  • The content of four phonolic acids 1-4, and two flavonol aglycones 14 and 15 from Orostachysjaponicus A. Berger grown under night-break and day-length controlled experiments was estimated and compared with those in wild plants. The amount of the phenolic acids 1-4 and the flavonol aglycones 14 and 15 increased with increasing light irradiation under both the night-break and day-length control conditions. It was disclosed that the cultivation conditions such as the night-break and the day-length control were not adversely affect the production of phenolic acids and flavonols in Orostachys japonicus A. Berger extracts.

Effects of Phenolic Compounds in Milled Barley Grains on the Growth of Saccharomyces cerevisiae (보리쌀중의 Phenol 화합물이 Saccharomyces cerevisiae의 생육에 미치는 영향)

  • 정기택;김욱한;송형익
    • Korean Journal of Microbiology
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    • v.24 no.2
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    • pp.168-174
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    • 1986
  • The phenolic compounds contained in milled barley grains were seperated and identified by gas liquid chromatography and the effects of phenolic compounds extracted from milled barley grains and each authentic phenolic compound on the growth of Saccharomyces cerevisiae were studied. Severn phenolic acids, namely cinnamic, protocatechuic, ferulic, sinapid, vanillic, syringic, gallic acids, were identified in milled barley grains by gas liquid chromatography. The contents of sinapic, ferulic, cinnamic, protocatechuic acids were larger than those of vanillic and gallic acids. Phenolic compounds, extracted from milled barley grains and supplemented in culture broth, were inhibitory to the growth of Saccharomyces cerevisiae at levels above 100ppm to 24 hours but not inhibitory at all levels after 48 hours. Cinnamic, ferulic, vanillic acids at all levels were inhibitory to the growth of Saccharomyces cerevisiae, among them cinnamic acid was most inhibitory. Syringic acid was inhibitory to the growth of the yeast at the initial stage of culture. But sinapic and protocatechuic acids were slightly stimulatory to the growth of the yeast and gallic acid was ineffective to the growth of the yeast.

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Fixed-bed Adsorption of Phenolic Acids on Charcoal in Multi Solutes System (활성탄을 이용한 다성분계 페놀산 용액의 고정층 흡착)

  • Lee, Won-Young;Choi, Yong-Hee
    • Current Research on Agriculture and Life Sciences
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    • v.15
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    • pp.83-91
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    • 1997
  • Phenolic acids are regarded as harmful materials in food and environment science. But recently, regarded as useful materials by their characteristics which bind metal ions and have pharmaceutical effect. It was necessary to remove or recover phenolic acids from solutIon containing phenolic acids. Continuous fixed-bed adsorption was adapted in order to separate phenolic acids from diluted solution and the breakthrough curve was predicted by nonlinear curve fitting method. The larger bed length showed the longer breakpoint time and the slow mass transfer coefficient. Ferulic acid among the phenolic acids was passed through the breakpoint first and the second and. third were p-coumaric acid and gallic acid. These orders were caused by not only ionic strength between adsrobent and adsorbate but also molecular weights.

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The Nitrite Scavenging and Electron Donating Ability of Phenolic Compounds (페놀성 화합물의 아질산염 소거 및 전자공여 작용)

  • Kang, Yoon-Han;Park, Yong-Kon;Lee, Gee-Dong
    • Korean Journal of Food Science and Technology
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    • v.28 no.2
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    • pp.232-239
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    • 1996
  • Phenolic compounds are known to inhibit the nitrosation or oxidation reaction. In the present work, the effects of phenolic compounds including phenolic acids and flavonoids on the nitrite-scavenging and electron donating ability were tested as scavenger of nitrite which is believed to participate in the formation of N-nitroso compounds and investigated as electron donator. The nitrite scavenging ability appeared in all the phenolic acids and showed the highest value at PH 1.2. Among the Phenolic compounds, phenolic acids showed higher nitrite-scavenging action than some flavonoids. Futhermore, the nitrite scavenging action of phenolic compounds was pH dependent highest at pH 1.2 and lowest at pH 6.0. The electron donating ability (EDA) by reduction of ${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) among hydroxybenzoic acids was in the decreasing order of gallic acid, gentisic acid, syringic acid, protocatechuic acid, salicylic acid, vanillic acid, benzoic acid and p-hydroxybenzoic acid. EDA of hydroxycinnamic acids was in the decreasing order of hydrocaffeic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid. EDA of flavonoids was in the decreasing order of (+)catechin, rutin, quercetin, naringin and hesperidin. Other phenolic compounds were significantly high in electron donating abilities.

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Composition and Antioxidative Characteristics of Phenolic Fraction Isolated from Soybean Fermented Food (콩 발효식품으로부터 분리한 페놀물질획분의 성분분석과 항산화 작용 특성)

  • 이정수;최홍식
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.3
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    • pp.383-389
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    • 1997
  • Doenjang(fermented soybean paste) was prepared by the series of processes including soaking, cooking, first fermentation(3 days at 30$\pm$2$^{\circ}C$) for the preparation of meju(soybean koji) after inoclulation of Aspergillus oryzae, and further fermentation (60 days at 30$\pm$2$^{\circ}C$) for the ripening after addition of salt 13% to meju. the crude phenolics extracted from defatted soybean and doenjang were fractionated onto the neutral phenolics(isofavonoids) and acidic phenolics(phenolic acids), respectively. Composition and antioxidative characteristics of phenolic fractions were determined. The neutral phenolic fractions contained genistin, genistein and daidzein; on the other hand, acidic phenolic fractions had syringic acid and seven other components. The content of genistin in doenjang dramatically decreased at the early stage of fermentation, whereas the content of genistein rather increased. In addition, the content of syringic acid of acidic phenolic fractions were increased during fermentation. These changes in individual phenolic components affected the antioxidative activity of neutral phenolics or acidic phenolics. antioxidative activity of phenolic compounds were evaluated during soybean fermentation. The antioxidative and free radical scavenging activity of neutral phenolic fractions and acidic phenolic fractions on linoleic acid autoxidation were also investigated.

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Isolation and Identification of Free Phenolic Acids in Korean Ginseng (인삼의 유리 페놀성 분획중 phenolic acid의 순수분리 동정)

  • Kim, Man-Wook;Wee, Jae-Joon;Park, Jong-Dae
    • Korean Journal of Food Science and Technology
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    • v.19 no.5
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    • pp.392-396
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    • 1987
  • A method for isolation of some phenolic acids from Korean ginseng(Panax ginseng C.A.Meyer)was studied using silicic acid column chromatography. preparative thin layer chromatography and high performance liquid chromatography. Two phenolic compounds were isolated and identified as ferulic acid, mp $156-157^{\circ}C$ and vanillic acid. mp $154-156^{\circ}C$ by spectral data of Mass and NMR spectroscopy.

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Quality Characteristics and Antioxidant Potential of Seeds of Native Korean Persimmon Genotypes

  • Kim, Il-Doo;Dhungana, Sanjeev Kumar;Kim, Hye-Ryun;Shin, Dong-Hyun
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.670-678
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    • 2017
  • Persimmon seeds contain considerable amounts of minerals, amino and organic acids, natural antioxidants and phenolic compounds. The objective of this study was to investigate quality characteristics and antioxidant potential of Korean persimmon seeds. The pH (4.88-4.94), color values, contents of minerals, free amino acids, organic acids, and phenolic compounds and DPPH free radical scavenging potentials of persimmon seed extracts significantly (p < 0.05) varied with the genotypes. This study showed that the seeds could be used as a source of different mineral elements (47.14-85.07 mg/kg) without any measureable amount of heavy metals such as arsenic, cadmium, lead and mercury. Similarly, considerable amounts of organic (1550.13-2413.08 mg/kg) and essential amino (50.85-54.03 mg/kg) acids and total phenolic compounds ($1227.91-1307.78{\mu}g$ gallic acid equivalent/g) were also found in the seed extracts, indicating their potential food value as a natural antioxidant. Results of the present study imply that prethanol-A, a food preservative, can be used as an effective extraction to obtain the minerals, organic and free amino acids, and phenolic compounds from the persimmon seeds, which possess a big potential to be commercially used in food, cosmetic and pharmaceutical industries.

Antioxidative Effect of Phenolic Acids in Defatted Perilla Flour on Soybean Oil (탈지들깨박 중 페놀산의 대두유에 대한 항산화 효과(I))

  • 조희숙;안명수
    • Korean journal of food and cookery science
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    • v.15 no.1
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    • pp.55-60
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    • 1999
  • Free phenolic acid, soluble phenolic acid ester and insoluble bound phenolic acid were extracted from defatted perilla flour. Their antioxidative effects were compared with those of BHA, AE and TBHQ for soybean oils by measuring acid and peroxide values at 60$^{\circ}C$ for 25 days. The patterns of these extracts were compared by using gas chromatography. Free phenolic acid and soluble phenolic acid ester extracts showed a higher antioxidative effects than BHA and AP. Among phenolic extracts, free phenolic acid showed the most effective antioxidant activity in soybean oil. Six types of free phenolic acid, 3 types of soluble phenolic acid ester, and 2 types of insoluble phenolic acid were found in the extract.

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Simultaneous Determination of Benzoic Acid, Caffeic Acid and Chlorogenic Acid in Seeds of Eriobotrya japonica and their Antibacterial Effect

  • Jeong, Jun-Mo;Lee, Kyoung-In;Kim, Sun-Min
    • Journal of Applied Biological Chemistry
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    • v.57 no.1
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    • pp.89-93
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    • 2014
  • We aim to develop a simple method for simultaneous and quantitative determination of benzoic acid, caffeic acid and chlorogenic acid in seeds of Eriobotrya japonica. In addition, antibacterial effect of these three phenolic acids was examined. A basic method is performed on the high performance liquid chromatography system coupled to an UV-detector (230 nm) and reverse phase C-18 column ($4.6{\times}150mm$, $5{\mu}m$). Each phenolic acid was confirmed via liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring with negative-ion electrospray ionization (ESI(-)) mode. It is demonstrated that the method was could be applied to samples for an analytical study of the phenolic acids. On the other hand, three phenolic acids in seeds of E. japonica exhibited antibacterial effect against several pathogenic bacteria. Of these, benzoic acid was found to have stronger antibacterial effect.