• 한국고분자학회:학술대회논문집
• /
• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
• /
• pp.346-346
• /
• 2006

Several types of dynamic atomic force microscopy such as tapping-mode, force modulation-mode are commonly cooperated by phase-contrast imaging techniques, which were interpreted as elastic contrast by mistake in the past and are nowadays regarded as the representation of energy dissipative processes. However, as theoretically reported, the situation is not so simple when the strong adhesive interaction is involved. Furthermore, elastic and viscous contributions are not easily divided in the case of polymeric systems. Thus, the interpretation of image contrast for them must be very carefully treated. In this study, we will demonstrate how such contrast mechanisms are complicated, using several miscible and immiscible polymer blend systems as model samples.

• 한국광학회:학술대회논문집
• /
• 한국광학회 2007년도 하계학술발표회 논문집
• /
• pp.69-70
• /
• 2007

• 한국산업보건학회지
• /
• 제1권2호
• /
• pp.144-153
• /
• 1991

This study was conducted to evaluate worker exposure to airborne asbestos fibers by industry, and to evaluate polarized-light microscopy for determining airborne asbestos fibers. A total of 11 plants including asbestos textile, brake-lining manufacturing, slate manufacturing, and automobile maintenance shops were investigated. Rsults of the study are summarized as follows. 1. Worker exposure levels to airborne asbestos fibers were the highest in asbestos textile industry, followed by brake-lining manufacturing, slate manufacturing, and automobile maintenance shops, in order. In asbestos textile industry, large variation of asbestos levels was found by plants. The worst plant indicated airborne fiber concentrations in excess of 10 fibers/cc, however, the best plant showed concentrations within 0.50 fibers/cc. 2. Characterization of airborne fibers by industry indicated that fibers from asbestos textile industry were the longest with the largest aspect ratio. Fibers from automobile maintenance shops were the shortest with the smallest aspect ratio. Based on characteristics of fibers and the highest levels of concentrations, it is concluded that workers in the asbestos textile industry are exposed to the highest risk of producing asbestosis, lung cancer, and mesothelioma. 3. Result s obtained using polarized-light microscopy were $43.7{\pm}12.3%$ of the results obtained using phase contrast microscopy. This may be resulted from the worse resolution of polarized-light microscopy than that of phase contrast microscopy. Based on the results, it is recommended that polarized-light microscopy be used for mainly bulk sample analyses and further study be performed to improve the method for determining airborne samples. However, polarized-light microscopy can be used for determining thick fibers.

• Applied Microscopy
• /
• 제38권1호
• /
• pp.63-72
• /
• 2008

Allen et al. (2004)에 의해 개발된 IWFR 방법에 대해서 응용성에 관심을 가지고 고찰되었다. 이 고찰에는 문헌에 보고된 GaAs, $YBa_2CuO_7$ 및 $Al_2CuMg$의 재료에 대한 HRTEM 연구물들이 이용되었다. 이 고찰 과정에서 이 방법의 타당성에 대한 이론적 근거, 제한조건 및 정보한계들을 명확히 제시되었다. IWFR 방법을 통해 얻은 결정 밑 표면에 전자 파동함수의 상(phase)-영상은 구면수차에 의한 영상 왜곡이 교정된 정보한계 범위 내에서 원자분해상을 나타낼 뿐만 아니라 결정의 구성원자의 원자번호에 대체적으로 비례하는 강도 분포를 나타내는 강한 경향이 있음이 특히 주목되었다.

• Current Applied Physics
• /
• 제18권11호
• /
• pp.1261-1267
• /
• 2018

In this paper, an off-axis digital holographic microscopy compensated with self-hologram rotation is presented. The process is implemented via subtracting the unwrapped phase maps of the off-axis parabolic hologram and its rotation $180^{\circ}$ to eliminate the tilt induced by the angle between the spherical object wave O and the plane reference wave R. Merit of the proposed method is that it can be done without prior knowledge of physical parameters and hence can reconstruct a parabolic hologram of $1024{\times}768$ pixels within tens of milliseconds since it doesn't require a digital reference wave. The method is applied to characterize rough gold bumps and the obtained results were compared with those extracted from the conventional reconstruction method. The comparison showed that the proposed method can characterize rough surfaces with excellent contrast and in realtime. Merit of the proposed method is that it can be used for monitoring smaller biological cells and micro-fluidic devices.

• 한국가시화정보학회:학술대회논문집
• /
• 한국가시화정보학회 2002년도 추계학술대회 논문집
• /
• pp.31-34
• /
• 2002

Imaging techniques using x-ray beam at high energies (>6KeV) such as contact radiography, projection microscopy, and tomography have been used to nondestructively discern internal structure of objects in material science, biology, and medicine. This paper introduces the x-ray micro-imaging method using 1B2 micro-probe line of PAL (Pohang Accelerator Laboratory). Cross-sectional information on low electron density materials can be obtained by probing a sample with coherent synchrotron x-ray beam in an in-line holography setup. Living organism such as plants, insects are practically transparent to high energy x-rays and create phase shift images of x-ray wave front. X-ray micro-images of micro-bubbles of $20\~120\;{\mu}m$ diameter in an opaque tube were recorded. Clear phase contrast images were obtained at Interfaces between bubbles and surrounding liquid due to different decrements of refractive index.

• KSTLE International Journal
• /
• 제1권2호
• /
• pp.69-75
• /
• 2000

The paper compares topography, phase contrast and force spectroscopy in atomic force microscopy data for evaluating the microheterogeneity of surface layer. The worn surface of ion-plated TiN coating was measured using both a laboratory-built and a commercial AFM. The results of analysis revealed structural and micromechanical heterogeneity of the worn surfaces. We demonstrated that the phase image allows relatively qualitative estimation of elastic modulus of the sample surface. The tribolayer formed in the worn surface possessed much lower stiffness than the original coating. It is shown that the most stable phase imaging is provided with a stiff cantilever. In this case, phase contrast is well conditioned, first of all, by microheterogeneity of elastic properties of the investigated surfaces. In this study an attempt was also made to correlate the results of phase imaging with that of the farce spectroscopy. The joint analysis of information on the surface properties obtained by the phase imaging and quantitative data measured with the force spectroscopy methods allows a better understanding of the nature of the surface micromechanical heterogeneity.

• 한국산업보건학회지
• /
• 제22권2호
• /
• pp.134-139
• /
• 2012

Objectives: This study was conducted to study the analytical variability of A & B counting rules in counting using a phase contrast microscope airborne fibers collected on filters in man-made mineral or vitreous fibers (MMMFs) industries. Methods: Fibers in filters were counted using A & B rules of NIOSH Method 7400. Intra-counter and inter-counter variations by fiber type and density were obtained. The types of MMMFs analyzed were glass wool fiber, rock wool fiber, slag wool fiber, and refractory ceramic fibers. The densities of fibers classified were <20 $fibers/mm^2,$ 20 - <50 $fibers/mm^2$, 50 - <100 $fibers/mm^2,$ and ${\geq}100$ $fibers/mm^2,$ respectively. Results: Intra-counter relative standard deviations by rule A were 0.084, 0.102, 0.071 for glass wool fibers, rock wool fibers and refractory ceramic fibers, and those by rule B were 0.139, 0.120 and 0.142, respectively. Inter-counter relative standard deviations by rule A were 0.281, 0.296, 0.180 for glass wool fibers, rock wool fibers and refractory ceramic fibers, and those by rule B were 0.396, 0.337 and 0.238, respectively. Conclusions: Intra-counter variation was not different significantly among fiber types (p>0.05), but B rule variation for ceramic fibers approximately 2 times greater than corresponding A rule estimates, and intra-counter and inter-counter variations were higher in the low fiber density.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제11권3호
• /
• pp.1670-1683
• /
• 2017

Mitotic event recognition is a crucial and challenging task in biomedical applications. In this paper, we introduce the slow feature analysis and propose a fully-automated mitotic event recognition method for cell populations imaged with time-lapse phase contrast microscopy. The method includes three steps. First, a candidate sequence extraction method is utilized to exclude most of the sequences not containing mitosis. Next, slow feature is learned from the candidate sequences using slow feature analysis. Finally, a hidden conditional random field (HCRF) model is applied for the classification of the sequences. We use a supervised SFA learning strategy to learn the slow feature function because the strategy brings image content and discriminative information together to get a better encoding. Besides, the HCRF model is more suitable to describe the temporal structure of image sequences than nonsequential SVM approaches. In our experiment, the proposed recognition method achieved 0.93 area under curve (AUC) and 91% accuracy on a very challenging phase contrast microscopy dataset named C2C12.

• Tribology and Lubricants
• /
• 제29권4호
• /
• pp.213-217
• /
• 2013

As commercial atomic force microscopy (AFM) probes made of Si and $Si_3N_4$ have low stiffness, it is difficult to induce sufficient elastic deformation on the surface of a specimen in a tapping mode. Therefore, high-guality phase contrast images can not obtained. On the other hand, a tungsten AFM probe has relatively higher stiffness than a commercial AFM probe. Accordingly, it is expected to provide an enhanced phase contrast image, which is an effective tool for achieving a better understanding of the micromechanical properties of worn surfaces and wear mechanisms. In this study, on electrochemical etching method was optimized to fabricate tungsten probe tips for an AFM. Electrochemical etching was performed by applying pulse waves with a 20% duty cycle at various voltages instead of only a DC voltage, which has been commonly used.