• Journal of Ecology and Environment
• /
• 제32권3호
• /
• pp.149-158
• /
• 2009

American sycamore seedlings were grown in chambers with two different ozone concentrations ($O_3$-free air and air with additional $O_3$) for 45 days. Both the control and the $O_3$ chambers included non-fertilized and fertilized plants. After 18 days of $O_3$ fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of $O_3$-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The $O_3$-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physiological inhibition, it does not seem to affect the growth response.

• BMB Reports
• /
• 제39권5호
• /
• pp.492-501
• /
• 2006

Since differentiation therapy is one of the promising strategies for treatment of leukemia, universal efforts have been focused on finding new differentiating agents. In that respect, we used guanosine 5'-triphosphate (GTP) to study its effects on K562 cell line. GTP, at concentrations between 25-200 ${\mu}M$, inhibited proliferation (3-90%) and induced 5-78% increase in benzidine-positive cells after 6-days of treatments of K562 cells. Flow cytometric analyses of glycophorine A (GPA) showed that GTP can induce expression of this marker in more mature erythroid cells in a time- and dose-dependent manner. These effects of GTP were also accompanied with inhibition of DNA synthesis (measured by [$^3H$]-thymidine incorporation) and early S-phase cell cycle arrest by 96 h of exposure. In contrast, no detectable effects were observed when GTP administered to unstimulated human peripheral blood lymphocytes (PBL). However, GTP induced an increase in proliferation, DNA synthesis and viability of mitogen-stimulated PBL cells. In addition, growth inhibition and differentiating effects of GTP were also induced by its corresponding nucleotides GDP, GMP and guanosine (Guo). In heat-inactivated medium, where rapid degradation of GTP via extracellular nucleotidases is slow, the anti-proliferative and differentiating effects of all type of guanine nucleotides (except Guo) were significantly decreased. Moreover, adenosine, as an inhibitor of Guo transporter system, markedly reduced the GTP effects in K562 cells, suggesting that the extracellulr degradation of GTP or its final conversion to Guo may account for the mechanism of GTP effects. This view is further supported by the fact that GTP and Guo are both capable of impeding the effects of mycophenolic acid. In conclusion, our data will hopefully have important impact on pharmaceutical evaluation of guanine nucleotides for leukemia treatments.

• 대기
• /
• 제27권1호
• /
• pp.105-118
• /
• 2017

This study assesses the performance of the Weather Research and Forecasting (WRF) model in reproducing regional climate over CORDEX-East Asia Phase 2 domain with different cumulus parameterization schemes [Kain-Fritch (KF), Betts-Miller-Janjic (BM), and Grell-Devenyi-Ensemble (GD)]. The model is integrated for 27 months from January 1979 to March 1981 and the initial and boundary conditions are derived from European Centre for Medium-Range Weather Forecast Interim Reanalysis (ERA-Interim). The WRF model reasonably reproduces the temperature and precipitation characteristics over East Asia, but the regional scale responses are very sensitive to cumulus parameterization schemes. In terms of mean bias, WRF model with BM scheme shows the best performance in terms of summer/winter mean precipitation as well as summer mean temperature throughout the North East Asia. In contrast, the seasonal mean precipitation is generally overestimated (underestimated) by KF (GD) scheme. In addition, the seasonal variation of the temperature and precipitation is well simulated by WRF model, but with an overestimation in summer precipitation derived from KF experiment and with an underestimation in wet season precipitation from BM and GD schemes. Also, the frequency distribution of daily precipitation derived from KF and BM experiments (GD experiment) is well reproduced, except for the overestimation (underestimation) in the intensity range above (less) then $2.5mm\;d^{-1}$. In the case of the amount of daily precipitation, all experiments tend to underestimate (overestimate) the amount of daily precipitation in the low-intensity range < $4mm\;d^{-1}$ (high-intensity range > $12mm\;d^{-1}$). This type of error is largest in the KF experiment.

• 한국건설관리학회논문집
• /
• 제16권3호
• /
• pp.3-10
• /
• 2015

건설 프로젝트의 VE는 크게 설계단계 VE와 시공단계 VE로 분류된다. 설계단계 VE는 정부의 법률에 의해 100억 원 이상의 공공 건설공사에 적용해야만 한다. 따라서 설계VE는 건설 프로젝트에 적극적으로 적용되고 있다. 이와는 대조적으로, 시공 VE는 제한된 특정 건설 프로젝트에 적용되고 있다. 그 이유는 시공 VE 프로세스가 실무적 요구 품질을 반영 할 수 없기 때문이다. 시공 VE는 시공 가능성에 대한 타당성 검증을 통해 긍정적인 효과를 구현할 수 있다. 이에 본 연구에서는 실무를 반영한 시공 VE의 방법을 제안하고자 한다. 제안 방법은 직관지수(G.F.I.)를 활용한 전문가 기반의 대안평가 방법이며, 제안 방법의 적용성을 검증하기 위해 실무사례에 적용하였다. 그 결과 제안된 대안평가 방법은 기존의 방법보다 적용 절차, 적용 방법 측면에서 효율성이 높은 것으로 나타났다.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제6권2호
• /
• pp.702-721
• /
• 2012

In the DVB-T2 system with a multiple-input single-output (MISO) transmission mode, Alamouti coded orthogonal frequency division multiplexing (OFDM) signals are transmitted simultaneously from two spatially separated transmitters in a single frequency network (SFN). In such systems, each transmit-receive link may have a distinct carrier frequency offset (CFO) due to the Doppler shift and/or frequency mismatch between the local oscillators. Thus, the received signal experiences dual CFOs. This not only causes dual phase errors in desired data but also introduces inter-carrier interference (ICI), which cannot be removed completely by simply performing a CFO compensation. To overcome this problem, this paper proposes an iterative detection with dual phase errors compensation technique. In addition, we propose a successive-iterative ICI cancellation technique. This technique successively eliminates ICI in the initial iteration by exploiting pre-detected data pairs. Then, in subsequent iterations, it performs a fine interference cancellation using a priori information, iteratively fed back from the channel decoder. In contrast to previous works, the proposed techniques do not require estimates of dual CFOs. Their performances are evaluated via a full DVB-T2 simulator. Simulation results show that the DVB-T2 receiver equipped with the proposed dual phase errors compensation and the successive-iterative ICI cancellation techniques achieves almost the same performance as ideal dual CFOs-free systems, even for large dual CFOs.

• Journal of Microbiology and Biotechnology
• /
• 제27권2호
• /
• pp.297-305
• /
• 2017

The use of peroxidase in the nitration of phenols is gaining interest as compared with traditional chemical reactions. We investigated the kinetic characteristics of phenol nitration catalyzed by horseradish peroxidase (HRP) in an aqueous-organic biphasic system using n-butanol as the organic solvent and ${NO_2}^-$ and $H_2O_2$ as substrates. The reaction rate was mainly controlled by the reaction kinetics in the aqueous phase when appropriate agitation was used to enhance mass transfer in the biphasic system. The initial velocity of the reaction increased with increasing HRP concentration. Additionally, an increase in the substrate concentrations of phenol (0-2 mM in organic phase) or $H_2O_2$ (0-0.1 mM in aqueous phase) enhanced the nitration efficiency catalyzed by HRP. In contrast, high concentrations of organic solvent decreased the kinetic parameter $V_{max}/K_m$. No inhibition of enzyme activity was observed when the concentrations of phenol and $H_2O_2$ were at or below 10 mM and 0.1 mM, respectively. On the basis of the peroxidase catalytic mechanism, a double-substrate ping-pong kinetic model was established. The kinetic parameters were ${K_m}^{H_2O_2}=1.09mM$, ${K_m}^{PhOH}=9.45mM$, and $V_{max}=0.196mM/min$. The proposed model was well fit to the data obtained from additional independent experiments under the suggested optimal synthesis conditions. The kinetic model developed in this paper lays a foundation for further comprehensive study of enzymatic nitration kinetics.

• 한국세라믹학회지
• /
• 제49권6호
• /
• pp.485-492
• /
• 2012

Dense and single phase $BiFeO_3$ (BFO) ceramics were prepared using attrition milled calcined (coarse) powders of an average particle size of ${\approx}3{\mu}m$ by conventional sintering process. A relative density of ${\approx}96%$ with average grain size $7.3{\mu}m$ was obtained when the powder compacts were sintered at $850^{\circ}C$ even for a shorter duration of 10 min. In contrast, densification barely occurred at $800^{\circ}C$ for up to 12 h rather the microstruce showed the growth of abnormal grains. The grain growth behavior at different temperatures is discussed in terms of nonlinear growth rates with respect to the driving force. The sample sintered at $850^{\circ}C$ for 12 h showed enhanced electrical properties with leakage current density of $4{\times}10^{-7}A/cm^2$ at 1 kV/cm, remnant polarization $2P_r$ of $8{\mu}C/cm^2$ at 20 kV/cm, and minimal dissipation factor (tan ${\delta}$) of ~0.025 at $10^6$ Hz. These values are comparable to the previously reported values obtained using unconventional sintering techniques such as spark plasma sintering and rapid liquid phase sintering.

• 한국운동역학회지
• /
• 제26권4호
• /
• pp.369-375
• /
• 2016

Objective: The purpose of this study was to investigate the possibility of injuries and the types of movement related to damage by body parts, and to prepare for prevention of injuries and development of a training program. Method: For this study, the experiment was conducted according to levels of 60 percentages (ST) and 85 percentages (MA) and 10 subjects from the Korean elite national weightlifting team were included. Furthermore, we analyzed joint moment and muscle activation pattern with three-dimensional video analysis. Ground reaction force and EMG analyses were performed to measure the factors related to injuries and motion. Results: Knee reinjuries such as anterior cruciate ligament damage caused by deterioration of the control ability for the forward movement function of the tibia based on the movement of the biceps femoris when the rectus femoris is activated with the powerful last-pull movement. In particular, athletes with previous or current injuries should perceive a careful contiguity of the ratio of the biceps femoris to the rectus femoris. This shows that athletes can exert five times greater force than the injury threshold in contrast to the inversion moment of the ankle, which is actively performed for a powerful last pull motion and is positively considered in terms of intentional motion. It is activated by excessive adduction and internal rotation moment to avoid excessive abduction and external rotation of the knee at lockout motion. It is an injury risk to muscles and ligaments, causing large adduction moment and internal rotation moment at the knee. Adduction moment in the elbow joint increased to higher than the injury threshold at ST (60% level) in the lockout phase. Hence, all athletes are indicated to be at a high risk of injury of the elbow adductor muscle. Lockout motion is similar to the "high five" posture, and repetitive training in this motion increases the likelihood of injuries because of occurrence of strong internal rotation and adduction of the shoulder. Training volume of lockout motion has to be considered when developing a training program. Conclusion: The important factors related to injury at snatch include B/R rate, muscles to activate the adduction moment and internal rotation moment at the elbow joint in the lockout phase, and muscles to activate the internal rotation moment at the shoulder joint in the lockout phase.

• The Plant Pathology Journal
• /
• 제18권6호
• /
• pp.328-332
• /
• 2002

Wheat samples showing typical spot blotch symptoms on stems and sheaths were collected from the field after physiological maturity, and were sealed in paper bags and stored in the laboratory at room temperature to study the survival of Bipolaris sorokiniana conidia on wheat straw. The materials were observed at monthly intervals to assess the conidia viability during storage. After 4 months, the frequency of individual conidia already present on wheat straw at the time of sampling was reduced and appeared to be progressively replaced by the formation of round structures consist-ing of conidia aggregates. After 5 months, distinct, individual conidia were no longer detected, and only 'clumps of conidia' were observed. These dark black aggregates or 'clumps of conidia’measured 157-170$\mu\textrm{m}$ in diameter and were grouped into boat-shaped olivacious conidia showing thick wall and measuring 50-82$\times$20-30$\mu\textrm{m}$. The germination was unipolar and below 0.5%, suggesting the occurrence of dormancy, In contrast, individual conidium produced on wheat during the growing season were 96-130$\times$16-20$\mu\textrm{m}$, slightly curved, hyaline to light pale, and euseptate with a bipolar germination reaching 98-100%. Bipolaris sorokiniana conidia produced on PDA were 55-82$\times$20-27$\mu\textrm{m}$, tapered at both ends, dark brown to olivacious, distoseptate, showed up to 1% germination, and were predominantly unipolar. Results of the present study suggest that B. sorokiniana conidia belonged to two different physiological categories corresponding to the pathogen's infection phase and its survival, respectively. The infection phase is characterized by a high germination percentage as opposed to the survival phase harboring apparent dormancy.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2009년도 제38회 동계학술대회 초록집
• /
• pp.361-361
• /
• 2010

The separated quasi-one-dimensional ($7{\times}7$) and ($5{\times}5$) phases on vicinal Si(557) surfaces were successfully realized by changing the crystallographic orientation and thermal treatment conditions. A small change in the crystallographic orientation of the Si(557) surface stabilized the quasi-one-dimensional ($5{\times}5$) phase of a (111) facet on vicinal Si(557) surfaces and made it coexist with a quasi-one-dimensional ($7{\times}7$) phase after an optimal thermal treatment, whereas only the quasi-one-dimensional ($7{\times}7$) phase was stable on the Si(557) surface. Interestingly, this causes the (111) terraces with different widths (L) to prefer only one of the $5{\times}5$ (L=12) and $7{\times}7$ (L=9) phases resulting in long-range order of both phases along the step edge direction, which was observed by scanning tunneling microscopy (STM) and was supported by first principle calculations. In contrast, the quasi-one-dimensional ($5{\times}5$ and ($7{\times}7$) phases were arranged randomly across the step edge direction. The change of surface morphology of vicinal Si(557) surfaces will be discussed with STM images and theoretical calculations by changing crystallographic cutting angles and thermal treatment conditions.