• Title/Summary/Keyword: phase-contrast

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THE EFFECTS OF CHLORHEXIDINE USING PATTERN WITH PERIODONTAL PACK ON CLINICAL AND MICROBIOLOGIC PARAMETER AFTER PERIODONTAL SURGERY (치주포대에 클로르헥시딘 이용형태가 치주수술후 임상과 미생물학적 변수에 미치는 영향)

  • Lee, Kang-Hyun;Kim, Sung-Ho;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.24 no.2
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    • pp.376-389
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    • 1994
  • A study was conducted to compare the effects of chlorhexidine mouthrinse and chlorhexidine acetate powder with periodontal pack during 4 weeks following periodontal surgery. Twelve patients were treated with Modified Widman flap procedures and devided into three groups of four patients each; control group, chlorhexidine mouthrinse group, chlorhexidine acetate powder group. Each group wasn't significant difference in clinical and microbiologic parameters at preoperation. Examination regarding plaque index, gingival index, pocket depth, change of gingival color postsurgically, pain index were performed by each methods and observed bacteria in gingiva by phase contrast microscope. Evaluations were made at 7, 14 and 28 days postsurgically. The results were as follows: l. In comparison of results revealed no significant differences in Plaque Index, Gingival Index, pocket depth and change of gingival color postsurgically. 2. The pocket depth of three experimental groups were significant reduced at 4 weeks. 3. Chlorhexidine mouthrinse group and chlorhexidine acetate powder group with periodontal pack were significant differences in pain index at 3, 4 days postsurgically. 4. Cocci and Non-motile rods were increased at 1, 2 weeks. But, motile rod and spirochete were increased at 4 weeks.

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THE EFFECTS OF CHLORHEXIDINE AND LISTERINE MOUTHRINSE WITHOUT PERIODONTAL DRESSING AFTER PERIODONTAL SURGERY (치주수술후 치주포대 비부착시 클로르헥시딘과 리스트린 구강 양치의 효과)

  • Lim, Yong-Soo;Kim, Yoon-Sung;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.24 no.2
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    • pp.406-420
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    • 1994
  • The purpose of this study was to compare the effects of Chlorhexidine, Listerine and hypertonic saline mouthrinse without periodontal pack during 2 weeks following periodontal surgery. Twelve patients were treated with modified Widman flap procedures. Each fourth patients assigned to chlorhexidine mouthrinse group, Listerine mouthrinse group and control group. Each group wasn't significant difference in clinical and microbiologic parameters at preoperation. Examination regarding plaque index, gingival index, pocket depth, change of gingival color postsurgically, pain index and contrast phase microscope were performed. Evaluations were made at the first, the second and the fourth weeks postsurgically. The results were as follows: 1. Chlorhexidine mouthrinse group and Listerine mouthrinse group were significantly different in plaque index at the first, the second and the fourth weeks 2. The pocket depth of three groups were significantly reduced at the fourth weeks. 3. Chlorhexidine mouthrinse group and Listerine mouthrinse group were significantly different in change of gingival color at the first week. 4. Cocci and non-motile rods were increased at the first and the second weeks. But, motile rods and spirochete were increased at the fourth weeks.

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Xylan 분해균주인 Bacillus stearothermophilus의 오탄당 이용

  • 이효선;조쌍구;최용진
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.385-392
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    • 1996
  • Bacillus stearotheymophilus, a potent xylanolytic bacterium isolated from soil, was tested for the strain's strategies of pentose utilization and the evidence of substrate preferences. The strain metabolized glucose, xylose, ribose, maltose, cellobiose, sucrose, arabinose and xylitol. The efficacy of the sugars as a carbon and energy source in this strain was of the order named above. The organism, however, could not grow on glycerol as a sole growth substrate. During cultivation on a mixture of glucose and xylose or arabinose, the major hydrolytic products of xylan, B. stearothermophilus displayed classical diauxic growth in which glucose was utilized during the first phase. On the other hand, the pentose utilization was prevented immediately upon addition of glucose. Cellobiose was preferred over xylose or arabinose. In contrast, maltose and pentose were co-utilized, and also no preference on between xylose and arabinose. Enzymatic studies indicated that B. stearothermophilus possessed constitutive hexokinase, a key enzyme of the glucose metabolic system. While, the production of $^{D}$-xylose isomerase, $^{D}$-xylulokinase and $^{D}$-arabinose isomerase essential for pentose phosphate pathway were induced by xylose, xylan, and xylitol but repressed by glucose. Taken together, the results suggested that the sequential utilization of B. stearothermophilus would be mediated by catabolite regulatory mechanisms such as catabolite inhibition or inducer exclusion.

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How to make spatially focused sound shape: wavenumber spectrum matching (공간 상에 원하는 음장형상을 만드는 방법)

  • Park, Jin-Young;Kim, Yang-Hann
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.11a
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    • pp.1014-1017
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    • 2007
  • Sound focusing technologies has been studied for various purposes from early 1990s. As a result, these technologies make us possible to apply in many uses. For example, we can treat tumors using focused ultrasonic waves without surgical knife and communicate in the ocean using time reversal array. Also applications for personal audio system become issues. Recently, as technologies are developing, in some applications, needs for regional focusing become increasing because previously suggested focusing methods, such as phase conjugation, time reversal and inverse filtering, were all about a point focusing. Therefore, studies on regional focusing method are essentially needed. Regional focusing method was firstly mentioned by Choi and Kim in 2002: acoustic contrast control. However, in regional focusing, physical interpretations between control variables and results are still not easy because of its complexity. In this regard, we tried to understand the relations between control variables and results in wavenumber domain and suggested a solution method for regional focusing: wavenumber spectrum matching. We also showed how to make spatially focused sound shape using the suggested method from the simplest case: line focusing.

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Identification of Beauveria spp. Isolated from Mulberry Longicorn Beetle (Apriona germari Hope) using Polymerase Chain Reaction (뽕나무 하늘소(Apriona germari Hope)로부터 Beauveria속 사상균의 분리 및 PCR에 의한 동정)

  • 서종복;진병래
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.167-171
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    • 1995
  • To develope a microbial pesticide for the control of mulberry longicorn beetle, Apriona germari, Beauveria spp. were isolated from the infected Apriona germari larvae. The morphology of Beauveria spp. was observed by phase contrast and scanning electron microscope. In addition, the Beauveria spp. isolated from Apriona germari were identified by the random amplification of polymorphic DNA using polymerase chain reaction. The results showed that the Beauveria spp., SFB-1A and SFB-3A, isolated from Apriona germari were identified with B. bassiana and B. brongniartii, respectively, suggesting that the random amplification of polymorphic DNA is effective for the identification of Beauveria spp.

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X-ray Micro-imaging of Arsenic Absorption of Sap Flow in Xylem Vessels of Pteris (X-ray 영상기법을 이용한 비소 흡수가 고사리 내부 수액 거동에 미치는 영향 연구)

  • Lee, Jin-Pyoung;Lee, Sang-Joon
    • Journal of the Korean Society of Visualization
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    • v.5 no.1
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    • pp.30-36
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    • 2007
  • The global environment is deteriorating at an alarming rate, despite of enhanced international environmental regulation. Many studies have been performed to reduce toxic pollutants. Recently, plant-based phytoremediation technology for moving toxic contaminants from soil and water has been receiving large attention. Arsenic-contaminated soil is one of the major pollutant sources for drinking water. Pteris erotica has been known as a hyper-accumulator of arsenic from soils. In this study, we investigated the effect of arsenic absorption on sap flow inside xylem vessels of Pteris. The synchrotron X-ray micro-imaging technique was employed to monitor the refilling process of water containing arsenic inside the xylem vessels of Pteris's leaves and stems non-invasively. The captured phase-contrast X-ray images show both anatomy of internal structure and transport of water inside Pteris. The exposure of Pteris to arsenic solution was found to increase largely the water raise speed in xylem vessels. The present results would provide important information needed for understanding the mechanisms of accumulation and transportation of toxic materials in plants.

Evaluation of Chest Density in Insight Film (Insight film을 이용한 농도측정 평가)

  • Kwon, Lee-Seon;Park, Jang-Hwan;Lim, Oh-Soo;Jung, Kyong-Mo;Cheung, Hwan
    • Journal of radiological science and technology
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    • v.15 no.2
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    • pp.17-23
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    • 1992
  • Simple chest radiography is the most frequently performed examination in radiologic practice. However, with transmitted dose through chest which has the problem of wide variations in absorption, conventional Film/Screen Combinations Method make it difficult to delineate lung field mediastinum & cardia portion and subdiaphragmatic structures. In order to overcome this problem, we have been tried several methods as follows. High Kilovoltage Technique, Rare Earth Screen/Wide Lattitude Film, Use of Compensating Filter and Digital Beam Attenuator and recently Insigh System has been developed. In this paper, we'd like to discuss Insight film through apperciation by measuring the difference of contrast. In order to evaluate insight film. We have done experiments using Insight System with Simple Phase, Picker 550. We could get an result which was able to overcome the limitted dynamic range of conventional Film/Screen System remarkably.

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Resveratrol Inhibits Nitric Oxide-Induced Apoptosis via the NF-Kappa B Pathway in Rabbit Articular Chondrocytes

  • Eo, Seong-Hui;Cho, Hongsik;Kim, Song-Ja
    • Biomolecules & Therapeutics
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    • v.21 no.5
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    • pp.364-370
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    • 2013
  • Resveratrol (trans-3,4'-trihydroxystillbene), a naturally occurring polyphenolic antioxidant found in grapes and red wine, elicits diverse biochemical responses and demonstrates anti-aging, anti-inflammatory, and anti-proliferative effects in several cell types. Previously, resveratrol was shown to regulate differentiation and inflammation in rabbit articular chondrocytes, while the direct production of nitric oxide (NO) in these cells by treatment with the NO donor sodium nitroprusside (SNP) led to apoptosis. In this study, the effect of resveratrol on NO-induced apoptosis in rabbit articular chondrocytes was investigated. Resveratrol dramatically reduced NO-induced apoptosis in chondrocytes, as determined by phase-contrast microscopy, the MTT assay, FACS analysis, and DAPI staining. Treatment with resveratrol inhibited the SNP-induced expression of p53 and p21 and reduced the expression of procaspase-3 in chondrocytes, as detected by western blot analysis. SNP-induced degradation of I-kappa B alpha ($I{\kappa}B-{\alpha}$) was rescued by resveratrol treatment, and the SN50 peptide-mediated inhibition of NF-kappa B (NF-${\kappa}B$) activity potently blocked SNP-induced caspase-3 activation and apoptosis. Our results suggest that resveratrol inhibits NO-induced apoptosis through the NF-${\kappa}B$ pathway in articular chondrocytes.

In Vivo Visualization of Flow in Xylem Vessels of a Bamboo Leaf Using Synchrotron X-ray Micro Imaging Technique (Synchrotron X-ray 미세영상기법을 이용한 식물 목질부 내부 수액 유동의 계측)

  • Kim, Yang-Min;Lee, Sang-Joon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.11
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    • pp.1612-1617
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    • 2003
  • Synchrotron X-ray micro imaging technique was employed to non-invasively monitor the water flow inside xylem vessels in a bamboo leaf. The phase contrast X-ray images clearly visualized plant anatomy and the rise of a water front inside the vessels. Consecutive X-ray images taken for 60 seconds revealed water rise kinetics against gravity in the xylem of a cut dry leaf taken from a bamboo tree. For the first time, traces of water rise, variation of contact angle between water and xylem wall as well as the internal structure of xylem were obtained. In xylem vessels, a repeating flow pattern has a typical flow velocity of 30.7$\mu\textrm{m}$/s and faster flow is established intermittently. It is concluded that the transmission type of X-ray micro imaging can be used as a powerful tool to investigate the ascent of sap in the xylem vessels at a resolution higher than that of MRI.

Microcalcification Extraction by Using Automatic Thredholding Based on Region Growing (영역 성장법을 기반으로 자동적인 임계치 설정을 이용한 미세 석회화 추출)

  • 원철호;권용준;이정현;박희준;임성운;김명남;조진호
    • Journal of Biomedical Engineering Research
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    • v.25 no.4
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    • pp.235-242
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    • 2004
  • In this paper, we proposed the algorithm for detection of microtalcification by automatic threshold decision based on region growing method. The region for optimal threshold is grown from local maximum pixel by increasing repeatedly threshold in microralcification candidate region. Then, the optimal threshold is automatically decided at the maximum value of the contrast and edge sharpness in this region. Microcalcifications could be efficiently detected as satisfied result that true positive ratio is 81.5% and average false positive numbers are 1.1 about total 299 microcalcifirations in real image. In a result, we showed that this algorithm can be used to aid diagnostic-radiologist for the diagnosis of the early phase of breast cancer.