• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.10B
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• pp.855-864
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• 2008

Optical link techniques compensating chromatic dispersion and nonlinear effects, which affect distortion of optical signals, generated in single mode fiber are investigated through computer simulation and design rule of these link techniques is proposed for implementation of wideband and long-haul WDM ($24{\times}40$ Gbps) transmission system. The optical link consist of dispersion management (DM) compensating the cumulated dispersion through total transmission line and optical phase conjugation in middle of total transmission line for compensating distorted signals by frequency inversion. DM schemes considered in this research are lumped DM and inline DM. It is confirmed that eye opening penalty (EOP) of overall WDM channels are more improved than those in WDM transmission systems with only optical phase conjugator (OPC), if DM is additionally applied to these systems. And, design rule in both DM schemes are proposed by using effective residual dispersion range. It is confirmed that inline DM is better than lumped DM in the improving EOP of total WDM channels and in effective residual dispersion range.

• KSBB Journal
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• v.18 no.4
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• pp.306-311
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• 2003

Solid-phase refolding of immobilized proteins can be an effective way to reuse an immobilized enzyme column. Oriented immobilization methods are known to provide higher activity of the immobilized enzymes. In this study, using recombinant EK (enterokinase) as a model enzyme and a fusion protein, that consisted of recombinant human growth hormone and six His tag that was linked by the peptide of EK-specific recognition sequence, as a model substrate, we evaluated two oriented immobilization methods, i. e., reductive alkylation of N-terminus ${\alpha}$-amine and affinity interaction between poly-histidine tag and Ni-NTA (nickel-nitrilotriacetic acid). The immobilization yield, activity and cleavage of the immobilized enzymes, and the yield of solid-phase refolding were compared. The Ni affinity immobilization and the covalent immobilization yields were about 100％ and 65％, respectively. But the specific activities were the same, about 50％ of that of the soluble enzyme. The cleavage rate by the covalently immobilized EK was higher than the soluble enzyme and the side reaction of cryptic cleavage was significantly decreased. Covalently immobilized EK showed almost 100％ refolding yield but the affinity immobilized EK showed only 70％ yield, which suggested the covalent conjugation provided more rigid ‘reference structure’ for the solid-phase refolding. The monomeric hGH could be easily obtained by capturing the cleaved poly Histidine tag by the Ni affinity column.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.26 no.1
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• pp.113-118
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• 2015

In this paper, a cancellation technique for electromagnetic waves is realized based on a retrodirective antenna array system. The system re-transmits a signal to the direction of the incoming signal, with the same frequency and opposite phase. For the experimental verification, a $2{\times}2$ retrodirective array system is designed and fabricated. Experimental results for a metallic cylinder show as much as 26-dB reduction in reflection.

• Journal of information and communication convergence engineering
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• v.9 no.1
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• pp.32-37
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• 2011

In dispersion management (DM) and optical phase conjugation applied into optical transmission links with multi fiber spans for minimizing the impact of nonlinearity and group velocity (GVD), implementation possibility of DM using only one fiber span for pre- or postcompensation was assessed as a function of duty cycle of RZ pulse and residual dispersion per span (RDPS). It is confirmed that DM with optimal net residual dispersion (NRD) controlled by only one fiber span could be sufficiently applied into optical transmission links, though optimal NRD is more increased than that in transmission links with the general DM scheme of pre- and postcompensation. Thus, it is expected that optical transmission system is simply designed and implemented by applying the proposed DM scheme into real optical transmission links. Also, it is confirmed that the advantageous duty cycle of RZ is 0.5 and RDPS is setting to be small value for the effective transmitting wide signal wavelength range in optical links with optimal NRD controlled by only one fiber span.

• Bulletin of the Korean Chemical Society
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• v.21 no.12
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• pp.1202-1206
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• 2000

Transmission of substituent effects through 5-membered heteroaromatic rings is investigated theoretically at the RHF/6-31+G and B3LYP/6-31+G levels using the equilibria for the $OH^- addition to five-membered heteroaromatic aldehydes (5MHA). The transmission efficiency (S) in 5MHA(A) increases in the order NH>O>S>PH but the order exactly reverses in 5MHA($T^-$). This is originated by the delocalizability of the ${\pi}$ lonepair on Y, $$n_\pi$(Y)$. A better correlation is obtained with ${\sigma}_p^-$ in the Hammett plots with positive slope, $p_z$ > 0, indicating that the substituent (Z) effects are not transmitted by a direct conjugation. The magnitude of $p_z$ for Y=NH is the largest among the heteroaromatic systems, which is consistent with the largest transmission efficiency change $({\Delta}S)$. The equilibria for the addition processes are favorable in the gas phase $({\Delta}G^o<0)$, which reverses to unfavorable in aqueous solution $({\Delta}G^o>0)$ due to the relatively large salvation energy of $OH^-$ in the initial state in aqueous solution. The orders of ${\Delta}G^o$ and $p_z$ in the gas phase are almost maintained in solution.

• BMB Reports
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• v.44 no.7
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• pp.423-434
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• 2011

A female hormone, estrogen, is linked to breast cancer incidence. Estrogens undergo phase I and II metabolism by which they are biotransformed into genotoxic catechol estrogen metabolites and conjugate metabolites are produced for excretion or accumulation. The molecular mechanisms underlying estrogen-mediated mammary carcinogenesis remain unclear. Cell proliferation through activation of estrogen receptor (ER) by its agonist ligands and is clearly considered as one of carcinogenic mechanisms. Recent studies have proposed that reactive oxygen species generated from estrogen or estrogen metabolites are attributed to genotoxic effects and signal transduction through influencing redox sensitive transcription factors resulting in cell transformation, cell cycle, migration, and invasion of the breast cancer. Conjuguation metabolic pathway is thought to protect cells from genotoxic and cytotoxic effects by catechol estrogen metabolites. However, methoxylated catechol estrogens have been shown to induce ER-mediated signaling pathways, implying that conjugation is not a simply detoxification pathway. Dual action of catechol estrogen metabolites in mammary carcinogenesis as the ER-signaling molecules and chemical carcinogen will be discussed in this review.

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.13-21
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• 2016

Since the direct-gradient (DG) method uses the Shack-Hartmann wave front sensor (SH-WFS), based on the phase-conjugation principle, for atmospheric compensation in free-space optical (FSO) communication, it cannot effectively correct high-order aberrations. While the stochastic parallel gradient descent (SPGD) can compensate the distorted wave front, it requires more calculations, which is sometimes undesirable for an FSO system. A hybrid compensation (HC) method is proposed by properly using the DG method and SPGD algorithm to improve the performance of FSO communication. Simulations show that this method can well compensate wave-front aberrations and upgrade the coupling efficiency with few computations, preferable correction results, and rapid convergence rate.

• 정보저장시스템학회:학술대회논문집
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• 2005.10a
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• pp.42-43
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• 2005

Higher density in the holographic storage can be obtained by using shorter wavelength laser and higher numerical aperture (NA). In this paper, we adopted a blue laser diode with a wavelength of 405nm and high NA object lens. And the phase conjugation method is applied to prevent image distortion due to high NA. The performance of the system has been shown experimentally.

• Bulletin of the Korean Chemical Society
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• v.18 no.2
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• pp.208-212
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• 1997

Theoretical AM1 MO studies are reported on the gas-phase deprotonations of N-methyl 4-((Y-phenylsulfonyl)pyridinium cations (Ⅳ) and N-(Y-benzyl) 4-((phenylsulfonyl)methyl pyridinium cations (Ⅴ) using NH3 as a base. Bronsted α values for deprotonation of Ⅳ and Ⅴ are 0.18 and 0.17, respectively. Bronsted β value of 0.53 is found for the deprotonation of Ⅳ and Ⅴ by substituted aniline bases. The negative transition state (TS) imbalances, Ⅰ(=α-β) < 0, are rationalized by the negative distance factor, Δd (=dp-dTS) < 0, where dp and dTS are the distance between anionic charge center and substituents in the product and in the TS, respectively. The inability of d-pπ-conjugation in Ⅳ, where instead n-σ* type sigma delocalization occurs, causes very little lag in the structural reorganization in the TS.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.