• Title/Summary/Keyword: pharmaceutical research

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Isolation and Identification of Inhibitory Compounds on TNF-$\alpha$ Production from Magnolia fargesii

  • Chae, Sook-Hee;Kim, Pyoung-Su;Cho, Jae-Youl;Park, Ji-Soo;Lee, Jae-Ho;Yoo, Eun-Sook;Baik, Kyong-Up;Lee, Jong-Soo;Park, Myung-Hwan
    • Archives of Pharmacal Research
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    • v.21 no.1
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    • pp.67-69
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    • 1998
  • Three TNF-$\alpha$inhibitory lignans were isolated from the flower buds of Magnolia fargesii through bioassay-guided isolation. They were identified as eudesmin, magnolin and lirioresinol-B dimethylether on the basis of their spectroscopic data. All three lignans showed inhibitory effects on TNF-$\alpha$ production in LPS-stimulated murine macrophage cell line, RAW264.7 and eudesmin showed the strongest activity ($IC_{50}=51{\mu}M)$.

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Analysis of DA-6034. a New Flavonoid Derivative in Biological Fluids by Fluorescence Detector

  • Jang, Ji-Myun;Park, Kyung-Jin;Lee, Jong-Jin;Kim, Dong-Goo;Shim, Hyun-Joo;Son, Mi-Won;Kim, Dong-Sung;Kim, Soon-Hoe;Yoo, Moo-Hi
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.403.2-403.2
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    • 2002
  • A high performance liquid chromatographic method was developed for the determination of DA-6034 in biological fluids using fluorescence detector. The method involved deproteinization of biological sample with the same volume of acetonitrile, 0.2M zinc sulphate. and 0.15M barium hydroxide. The aliquot of supernatant was injected onto Nova-pak C18 column and detected by fluorescence detector. Emission and excitation wavelength of detector were 336nm and 440nm. (omitted)

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Synthesis of N,N',N"-Trisubstituted Thiourea Derivatives and their Antagonistic Effect on the Vanilloid Receptor

  • Park, Hyeung-Geun;Park, Mi-Kyung;Choi, Ji-Yeon;Choi, Sea-Hoon;Lee, Ji-Hye;Suh, Young-Ger;Cho, Ha-Won;Oh, Uh-Taek;Lee, Jee-Woo
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.253.1-253.1
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    • 2003
  • Twenty-seven N,N',N"-trisubstituted thiourea derivatives were prepared. Among them, 1- [3-(4'-hydroxy-3'-methoxy-phenyl)-propyl] -1,3-diphenethyl-thiourea (81, IC$\sub$50/= 0,32 $\mu\textrm{m}$), showed 2-fold higher antagonistic activity than that of capsazepine (3, IC$\sub$50/= 0,65 $\mu\textrm{m}$) against the vanilloid receptor in a Ca$\^$2+/ -influx assay.

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