• The Korean Journal of Food And Nutrition
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• v.11 no.3
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• pp.354-358
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• 1998

The influence of hot water extracts and tannin obtained from persimmon leaves on xanthine oxidase were investigated. Above two samples had higher inhibitory effect against xanthine oxidase by hot water extracts and tannin obtained from persimmon leaves was 92.4% and 92.1% by addition of 2.0 mg/$m\ell$ of the hot water extracts and the tannin, respectively. The inhibitions by the hot water extracts and the tannin were of competitive mode with respect to xanthine as a substrate.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.6
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• pp.880-886
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• 1995

Antimutagenic effects of boiled water extract and tannin from persimmon leaves were studied by using Ames test, spore rec assay and SOS chromotest. Strong antimutagenic activities toward aflatoxin B1(AFB1), dimethyl-aminobiphenyl(DMAB), N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitroquinoline-1-oxide(4-NQO) were observed when boiled water extract and tannin from the persimmon leaves were added in the Salmonella typhimurium TA 100. In spore rec assay using Bacillus subtilis H17($rec^{+}$) and M45($rec^{-}$), boiled water extract and tannin from the persimmon leaves considerably inhibited the mutagenesis induce by MNNG. In SOS chromotest using Escherichia coli PQ37, these samples also exhibited strong antimutagenic activity toward 4-NQO. The tannin was more effective than boiled water extract of persimmon leaves in the antimutagenicity tests.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.4
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• pp.250-257
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• 2005

This study was conducted to investigate the effect of meteorological elements on the changes of inorganic nutrient contents in leaves of 'ruyu' and 'Nishimurawase' sweet persimmon during the growing season. The contents of N, P, K and Cu decreased in leaves of 'Fuyu' and 'Nishimurawase' sweet persimmon during vegetative growth, while Ca, Mg and Mn increased. The K content was higher in leaves of 'Fuyu' than in 'Nishimurawase' sweet persimmon. The P and Ca content was higher in 'Nishimurawase' leaves than in 'Fuyu' sweet persimmon. Mean temperature was positively correlated with content of P in leaves. Rainfall was positively correlated with the content of Ca in leaves, whereas it was negatively correlated with content of N and Cu in leaves. The duration of sunshine was positively correlated with N and Cu in leaves but negatively correlated with that of Ca, Mg, and Mn content.

• Food Science and Preservation
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• v.4 no.3
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• pp.323-330
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• 1997

Changes in antioxidative substance levels in eleven different cultivars of persimmon leaves during growth were investigated. In general, the contents of soluble phenols, L-ascorbic acid and flavonoids in astringent persimmon leaves(APL) were higher than those of nonastringent persimmon leaves(NAPL). The soluble phenol contents in APL and NAPL showed a tendency to decrease throughout leaf growth. L-ascorbic acid content in APL decreased rapidly during growth, whereas its content in NAPL reached its highest value at the late of July, and then decreased rapidly. Major flavonoids in APL and NAPL were quercetin and Kaempferol which were present in conjugate forms. Before acid hydrolysis, the contents of kaempferol and quercetin in APL and NAPL remained at a relatively constant level until the late of July, and then decreased slightly. After acid hydrolysis, kaempferol contents in APL and NAPL varied significantly by cultivar and growth stage, while quercetin contents decreased slowly until the late of July, and then increased drastically, reached a maximum at the early of August, afterward continuously decreased. These results suggest that APL harvested at the early of June may be useful as potential sources of natural antioxidants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.5
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• pp.865-870
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• 1996

Antitumor effects of hexane, chloroform fractions and tannins from persimmon leaves were studied by using sarcoma-180 tumor cells. The solid tumor growth was inhibited most effectively when 0.5mg/kg of hexane fraction was administerated to the Balb/c mouse. The life prolongation effects were 35.6%, 11.5% and 29.9%, respectively when hexane fraction, chloroform fraction and tannins from the persimmon leaves were administerated to the mouse. Spleen index, a marker for immunological activity, was increased in mice administrated hexane fraction, chloroform fraction and tannins of persimmon loaves, compared with the control group.

• Food Science and Preservation
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• v.7 no.1
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• pp.99-102
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• 2000

Comparing and analysing the productivity and the main ingredients of persimmon leaves by cultivars were conducted to improve the quality and productivity of the per-simmon leaf tea. As a result of the survey, Dungsi was decidedly good in growth co-ndition of survival rate, stem diameter and the number of shoots etc. Also , DUngsi had the highest leaf productivity of 567 leaves and the leaf weight of 1.98 kg per one plant after three years of planting . On accounts of productivity, ingredient analysis and panel test etc. we collectively through that Dungsi was the best cutltivar for the persimmon leaf tea.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1304-1308
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• 2008

This study was performed to evaluate the effect of persimmon leaves extract on the reactive oxygen species (ROS) in cultured melanoma cells. The B16/F10 melanoma cells were treated with various concentrations of t-butyl hydroperoxide (t-BHP). And also, the effect of persimmon leaves (PL) extract on the cytotoxicity mediated by t-BHP was done on the cell viability, tyrosinase activity and melanogenesis by colorimetric assays. In this study, t-BHP decreased cell viability in dose-dependent manner and XTT90 and XTT50 values were measured at 10 and 35 uM of PL, respectively in these culture. And also, XTT50 value was assessed as a highly toxic effect on cultured melanoma cells by the toxic criteria. In the effect of PL extract on the t-BHP-mediated cytotoxicity, PL extract significantly increased the cell viability injured by t-BHP in cultured B16/F10 melanoma cells. PL also showed the decreased tyrosinase activity and melanogenesis. From these results, it is suggested that ROS such as t-BHP showed highly toxic effect on cultured melanoma cells, and also, PL extract inhibited the tyrosinase activity and melanogenesis in cultured melanoma cells injured by ROS.

• Journal of the East Asian Society of Dietary Life
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• v.11 no.6
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• pp.472-478
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• 2001

This study was conducted to investigate the change in volatile flavor components of persimmon leaves during growth. The flavor components of persimmon leaves were analyzed by gas chromatography (GC) and combined gas chromatography-mass spectrometry(GC/MS). The flavor compounds were collected by simultaneous steam distillation and extraction(SDE) method, and were separated and identified resulting in 126 components, including 23 alcohols, 18 aldehydes, 4 esters. 15 acids, 37 hydrocarbons, 14 ketones, 6 phenols. and 9 others in persimmon leaves. The most abundant components of persimmon leaf were alcohols including iinalool. cis-3-hexanal. 1-$\alpha$ -terpineol. 3.7.11.15-tetramethyl-2-hexadecen-1-ol and aldehydes including trans-2-hexanal. nonanal, 2-decenal and hydrocarbons including 1.1-dimethylethyl cyclohexane, 1-methyl-4-(2-methylpropyl) -benzene. During growth, many other components were formed and dissipated after the 20th of June.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.3
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• pp.334-339
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• 1993

Antitumor activities of tannin extract and chloroform fraction extract from the persimmon leaves, and 2, 4-decadienal identified as an antimutagenic compound in persimmon leaves were examined in sarcoma 180 implanted tumor in mice by using both light and transmission electron microscopes. Among them, tannin extracted from the persimmon leaves delayed the progression of malignant tumor but the other two did not show any noticeable effect. The antitumorigenic activity of tannin extract might not come from the selective cytotoxicity against tumor cells, but might be, in an anaerobic environment, from the inhibitory action against oncogenic protein synthesis or from the proteolysis of the preformed oncogenic proteins by autophagocytic granules. therefore, the tannin from persimmon leaves might protect cells from fast progression of malignant tumorigensis.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.1
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• pp.32-37
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• 2002

In order to promote the utilization of persimmon leaf as food, chemical components in freeze dried Persimmon leaves were analyzed. The proximate composition was composed of moisture(79.65%), crude protein(17.97%), crude fat(1.33%), ash(2.37%), crude fiber(2.01%). During growth, moisture content was decreased and crude fat, crude fiber and ash were increased, respectively. Free sugar was composed of glucose, fructose, sucrose, raffinose. Nonvolatile organic acid contents were composed of oxalic acid, levulenic acid, magic acid and citric acid in the order. 18 amino acids of total amino acid in persimmon leaves were detected and major amino acid were glutamic acid, aspartic acid, leucine, phenylalanine, arginine and valine. The soluble tannin and vitamin C of persimmon leaves were 6859.37 mg% and 1487.12 mg%. During growth, its contents increased and then decreased after 20th June.