• 동의생리병리학회지
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• 제20권4호
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• pp.980-984
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• 2006

In order to examine the effect of oxygen free radicals on the vascular endothelial cells, cell viability was measured by XTT assay after bovine pulmonary vascular endothelial cell line(BPVEC) was treated only with hydrogen peroxide. In addition, the antioxidant effect of allopurinol on cells treated with hydrogen peroxide was examined by colormetric assay. in this study, the BPVEC treated with hydrogen peroxide showed the significantly decreased cell viability compared with control. Whereas, the viability of cells treated with hydrogen peroxide and allopurinol has significantly increased when compared with that of cells treated only with hydrogen peroxide. These results suggested that hydrogen peroxide, one of the oxygen free radicals showed cytotoxic effect and allopurinol has protective effect on oxygen free radical-induced cytotoxicity.

• 대한본초학회지
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• 제38권4호
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• pp.11-19
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• 2023

Objectives : The aim of this study was to investigate the effect of baicalein (BA) on the production of hydrogen peroxide and nitric oxide (NO) in RAW 264.7 mouse macrophages stimulated with polyinosinic-polycytidylic acid (poly-IC) and lipoteichoic acid. Methods : RAW 264.7 co-stimulated with poly-IC and lipoteichoic acid were incubated with baicalein at concentrations of 25 and 50 μM. Incubation time is 16 h, 18 h, 20 h, 22 h, and 24 h. After incubation, The production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Chrysin was used as a comparative material. NO production was evaluated by griess assay. Results : For 16 h, 18 h, 20 h, 22 h, and 24 h incubation, BA at the concentration of 25 and 50 μM significantly inhibited the production of hydrogen peroxide in RAW 264.7 stimulated by poly-IC and lipoteichoic acid (p <0.001). In details, production of hydrogen peroxide in 'poly-IC and lipoteichoic acid'-stimulated RAW 264.7 treated for 16 h with BA at concentrations of 25 and 50 μM was 82.36% and 77.24% of the control group treated with poly-IC and lipoteichoic acid only, respectively; the production of hydrogen peroxide for 18 h was 83.15% and 77.91%, respectively;production of hydrogen peroxide for 20 h was 82.88% and 77.82%, respectively; production of hydrogen peroxide for 22 h was 83.27% and 78.17%, respectively; production of hydrogen peroxide for 24 h was 83.54% and 78.35%, respectively. Additionally, BA at the concentration of 50 and 100 μM significantly inhibited NO production in lipoteichoic acid-induced RAW 264.7 (p <0.001). Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in 'poly-IC and lipoteichoic acid'-stimulated RAW 264.7 macrophages.

• 사상체질의학회지
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• 제14권1호
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• pp.79-89
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• 2002

To evaluate the effect of Yuldahansotang(YHT) water extract on cultured hippocampal cell was inhibited by hydrogen peroxide, MTT assay, NR assay, Neurofilament enzymeimmuno assay and DNA synthesis assay were carried out after the cultured hippocampal cells were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of hydrogen peroxide. The results obtained were as follows: 1. Hydrogen Peroxide decreased the survival rate of the cultured hippocampal cells on NR assay and MIT assay. 2. YHT water extract have efficacy of increasing a amount of neurofilament decreased by hydrogen peroxide in cultured hippocampal cells. 3. YHT water extract have efficacy of increasing DNA synthesis decreased by hydrogen peroxide in cultured hippocampal cells. From above the results, It is concluded that YHT has marked efficacy in preventing for the damages by hydrogen peroxide.

• Restorative Dentistry and Endodontics
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• 제27권4호
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• pp.441-447
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• 2002

The bleaching of discolored nonvital teeth is conservative treatment that satisfy the cosmetic desire. The most common method for this treatment, walking bleaching, is using 30% hydrogen peroxide and sodium perborate. Many alternatives are suggested for preventing the external cervical root resorption that is the common complication of the nonvital teeth bleaching with 30% hydrogen peroxide The same extent of oxidation reactions as that resulted by the bleaching with the application of 30% hydrogen peroxide and sodium perborate can also be acquired more safely by materials that contain 10% carbamide peroxide, used primarily for the bleaching of vital teeth. Therefore, this study was performed to evaluate the efficacy of 10% and 15% carbamide peroxide bleaching gel in nonvatal teeth bleaching. The internal bleaching of intentionally discolored teeth was performed in vitro with 10% carbamide peroxide (Group 1), 15% carbamide peroxide (Group 2), mixture of distilled water and sodium perborate (Group 3), and mixture of 30% hydrogen peroxide and sodium perborate (Group 4). The bleaching materials were refreshed following 3, 6, 9 and 12 days. To evaluate the bleaching effect, the color change of the crowns was measured at 1, 2, 3, 4, 7 and 15 days of bleaching using the colorimeter. The results were as follows：1. L$^*$ and $\Delta$E$^*$ values were increased with time in all bleaching agents (p<0.01). 2. There was no significant difference in L$^*$ and $\Delta$E$^*$ value among bleaching agents. 3. $\Delta$E$^*$ value higher than 3 was shown after 3 days of bleaching with 10% carbamide peroxide gel, 1 day with 15% carbamide poroxide gel, 4 days with mixture sodium perborate and distilled water and 4 days with mixture sodium perborate and 30% hydrogen peroride, respectively. These results revealed that the use of 10% and 15% carbamide peroxide bleaching gel in non-vital teeth bleaching is as effective as mixture of distilled water and sodium perborate and mixture of 30% hydrogen peroxide and sodium perborate. Accordingly, carbamide peroxide could be used clinically to bleach discolored non-vital teeth.

• Journal of Dairy Science and Biotechnology
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• 제22권2호
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• pp.107-112
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• 2004

과산화수소 형태의 반응성 산소에 대한 Lactobacillus Spp., Bifidobacterium spp. 및 Bacillus coagulans의 저항성과 세포 내에서 생성능력을 측정하기 위하여 본 연구가 수행되었다. Lactobacillus spp. 중에서 높은 과산화수소 저항성을 나타낸 균주는 L. acidophilus CU4111와 L. casei Cu4114인 것으로 나타났고 가장 낮은 저항성을 보인 균주는 L. brevis Cu4206이었다. Bifidobacterium longum CU4131은 높은 수준의 저항성을 나타내며 Bacillus coagulans를 포함하는 포자형성유산균주들은 전반적으로 과산화수소에 대한 저항성 이 높은 것으로 나타났다. 세포질 추출액 중의 과산화수소 함유 농도는 Bifidobacterium bifidum CU 4134가 가장 높고 Lactobacilli spp.의 세포질 추출액 중의 과산화수소 함유 농도는 L. casei CU 4114가 가장 높은 것으로 나타났다.

• 동의생리병리학회지
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• 제17권5호
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• pp.1202-1207
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• 2003

In order to clarify the neuroprotective effect of Cornu Saigae Tataricae(CST) water extract on cultured mouse spinal motor neuron damaged by hydrogen peroxide (H₂O₂), MTT [3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltetrazolium bromide] assay, LDH (Lactate Dehydrogenase) activity assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal motor neuron were preincubated with various concentrations of CST water extract for 3 hours prior to exposure of hydrogen peroxide Cell viability of cultured mouse spinal motor neurons exposed to various concentrations of hydrogen peroxide for 6 hours was decreased in a dose-dependent manner. MTT50 values were 40 uM hydrogen peroxide. Cultured mouse spinal motor neurons in the medium containing various concentration of hydrogen peroxide for 6 hours showed increasing of LDH activity and decreasing of total protein synthesis. We know that hydrogen peroxide was toxic on cultured spinal motor neurons. Pretreatment of CST water extract for 3 hours following hydrogen peroxide prevented the hydrogen peroxide-induced neurotoxicity such as increasing of LDH activity and decreasing of total protein synthesis. These results suggest that hydrogen peroxide shows toxic effect on cultured spinal motor neurons and CST water extract is highly effective in protecting the neurotoxicity induced by hydrogen peroxide.

• 한국환경보건학회지
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• 제31권2호
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• pp.115-119
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• 2005

This study was performed to investigate inhibitory effect on growth of Staphylococcus aureus and Bacillus cereus in lactic acid, hydrogen peroxide and combination of lactic acid and hydrogen peroxide. The minimun inhibitory concentration (MIC) of lactic acid in Staphylococcus aureus were 2500 ppm at pH 7.0, 1250 ppm at pH 5.5, 6.0 and 6.5, while in Bacillus cereus 625 ppm at pH 5.5 and 6.0, 1250 ppm at pH 6.5 and 7.0, respectively. MICs of hydrogen peroxide in Staphylococcus aureus were 50 ppm at pH 6.0, 75 ppm at pH 6.5 and 7.0, while in Bacillus cereus was 75 ppm at pH 5.0, 5.5 and 6.0, respectively. MICs of combined treatment of lactic acid and hydrogen peroxide in Staphylococcus aureus were 1250 ppm of lactic acid with 25 ppm of hydrogen peroxide and 625 ppm of lactic acid with 50 ppm of hydrogen peroxide. When Bacillus cereus were with 1250 ppm of lactic acid with 50 ppm of hydrogen per-oxide and 625 ppm of lactic acid with 75 ppm of hydrogen peroxide at 6.5. The correlations between MICs of lactic acid and hydrogen peroxide in S. aureus and B. cereus obtained through the coefficient of determination ($R^2$). $R^2$ value were 0.9934 and 0.9986, respectively. The inhibitory effect of lactic acid and hydrogen peroxide in S. aureus and B. cereus could be confirmed from the result of this experiment.

• 대한본초학회지
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• 제36권5호
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• pp.117-123
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• 2021

Objectives : The aim of this study is to investigate the effect of wogonin on the production of hydrogen peroxide in polyinosinic:polycytidylic acid (poly I:C)-stimulated TM4 mouse sertoli cells. Methods : TM4 were treated with poly I:C (50 ug/mL) and wogonin at concentrations of 5, 10, 25, and 50 µM for 30 min, 2 hr, 12 hr, 18 hr, and 24 hr. The production of intracellular hydrogen peroxide was measured by dihydrorhodamine 123 assay. Results : For 30 min, 2 hr, 12 hr, 18 hr, and 24 hr treatment, wogonin significantly inhibited intracellular hydrogen peroxide productions of TM4 at the concentration of 5, 10, 25, and 50 µM (p<0.05). In details, production of hydrogen peroxide in poly I:C-stimulated TM4 treated for 30 min with wogonin at concentrations of 5, 10, 25, and 50 µM was 95.67%, 92.69%, 92.05%, and 91.97% of the control group treated with poly I:C only, respectively; the production of hydrogen peroxide for 2 hr was 94.44%, 94.41%, 93%, and 92.98%, respectively; production of hydrogen peroxide for 12 hr was 96.78%, 95.32%, 94.33%, and 93.17%, respectively; production of hydrogen peroxide for 18 hr was 94.7%, 93.4%, 93.38%, and 93.35%, respectively; and production of hydrogen peroxide for 24 hr was 95.75%, 94.77%, 94.58%, and 92.8%, respectively. Conclusions : Wogonin might have anti-viral property related with its inhibition of intracellular hydrogen peroxide production in poly I:C-stimulated TM4 cells.

• 대한본초학회지
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• 제38권1호
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• pp.1-9
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• 2023

Objectives : The aim of this study was to investigate the effect of baicalein (BA) on the production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 mouse macrophages. Methods : Peptidoglycan-stimulated RAW 264.7 were incubated with baicalein at concentrations of 50 and 100 µM. Incubation time is 30 min, 2 h, 12 h, and 18 h. After incubation, The production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Berberine and gallic acid were used as the comparative materials. Results : BA at the concentration of 50 and 100 µM did not show cytotoxicity on RAW 264.7 for 24 h incubation. For 30 min, 2 h, 12 h, and 18 h incubation, BA at the concentration of 50 and 100 µM significantly inhibited the production of hydrogen peroxide in RAW 264.7 stimulated by peptidoglycan (p<0.05). In details, production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 treated for 30 min with BA at concentrations of 50 and 100 µM was 93.91% and 93.52% of the control group treated with peptidoglycan only, respectively; the production of hydrogen peroxide for 2 h was 93.8% and 92.71%, respectively; production of hydrogen peroxide for 12 h was 94.86% and 95.93%, respectively; production of hydrogen peroxide for 18 h was 95.37% and 96.48%, respectively. Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in peptidoglycan-stimulated RAW 264.7 macrophages.

• 대한본초학회지
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• 제39권4호
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• pp.37-45
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• 2024

Objectives : This study aimed to elucidate antioxidant activity of chrysin in polyinosinic-polycytidylic acid (poly-IC) and lipoteichoic acid-induced RAW 264.7 mouse macrophages. Methods : RAW 264.7 co-stimulated with poly-IC and lipoteichoic acid were incubated with chrysin at concentrations of 25 and 50 µM. Hydrogen peroxide production was measured with dihydrorhodamine 123 assay. Nitric Oxide (NO) production was evaluated by griess reagent assay. Results : For 16 h, 18 h, 20 h, 22 h, and 24 h incubation, chrysin at the concentration of 25 and 50 µM significantly suppressed hydrogen peroxide production in poly-IC and lipoteichoic acid-induced RAW 264.7. In details, production of hydrogen peroxide in 'poly-IC and lipoteichoic acid'-stimulated RAW 264.7 treated for 16 h with chrysin at concentrations of 25 and 50 µM was 83.84% and 79.3% of the control group treated with poly-IC and lipoteichoic acid only, respectively; the production of hydrogen peroxide for 18 h was 84.36% and 79.93%, respectively; production of hydrogen peroxide for 20 h was 85.68% and 80.22%, respectively; production of hydrogen peroxide for 22 h was 85.81% and 79.95%, respectively; production of hydrogen peroxide for 24 h was 86.01% and 80.18%, respectively. Additionally, chrysin at the concentration of 5, 10, 25, and 50 µM significantly inhibited NO production in THP-1 human monocytic cell line. Conclusions : Chrysin might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in 'poly-IC and lipoteichoic acid'-stimulated RAW 264.7.