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Protective Effects of Cornu Saigae Tataricae Extracts on Cultured Spinal Motor Neurons Damaged by Oxygen Free Radical  

Kang Gil Seong (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.5, 2003 , pp. 1202-1207 More about this Journal
Abstract
In order to clarify the neuroprotective effect of Cornu Saigae Tataricae(CST) water extract on cultured mouse spinal motor neuron damaged by hydrogen peroxide (H₂O₂), MTT [3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltetrazolium bromide] assay, LDH (Lactate Dehydrogenase) activity assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal motor neuron were preincubated with various concentrations of CST water extract for 3 hours prior to exposure of hydrogen peroxide Cell viability of cultured mouse spinal motor neurons exposed to various concentrations of hydrogen peroxide for 6 hours was decreased in a dose-dependent manner. MTT50 values were 40 uM hydrogen peroxide. Cultured mouse spinal motor neurons in the medium containing various concentration of hydrogen peroxide for 6 hours showed increasing of LDH activity and decreasing of total protein synthesis. We know that hydrogen peroxide was toxic on cultured spinal motor neurons. Pretreatment of CST water extract for 3 hours following hydrogen peroxide prevented the hydrogen peroxide-induced neurotoxicity such as increasing of LDH activity and decreasing of total protein synthesis. These results suggest that hydrogen peroxide shows toxic effect on cultured spinal motor neurons and CST water extract is highly effective in protecting the neurotoxicity induced by hydrogen peroxide.
Keywords
Cornu Saigae Tataricae; Hydrogen Peroxide(H₂O₂); MTT; NR; LDH; SRB;
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Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
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