• 대한수의학회지
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• 제64권2호
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• pp.15.1-15.5
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• 2024

A 13-year-old, Maltese dog presented with syncope and lethargy. Abdominal ultrasonography demonstrated anechoic peritoneal effusion and hepatic congestion. A focal echogenic round mass compressing the right ventricle and atrium was observed on echocardiography. Cardiac tamponade and right ventricular outflow tract obstruction occurred. On computed tomography, a homogeneous soft-tissue structure compressing the right chamber without contrast enhancement, suspected to be loculated pericardial effusion. During pericardiocentesis, cardiac tamponade was resolved, and irregular pericardial thickening was noted. Pericardial effusion was exudate and gram-positive bacterial colonies were observed on cytology. A diagnosis of fibrinous pericarditis secondary to bacterial infection was established.

• Archives of Pharmacal Research
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• 제11권3호
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• pp.203-212
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• 1988

To find antitumor components from the higher fungi of Korea, the mycelia of Favolus alveolarius (Fr.) Quelet were cultured in a liquid medium. The cultured mycelia were extracted with hot water twice, and a high molecular weight fraction was obtained by adding two volumes of ethanol to the extract. Two grams of Fraction A were obtained by dialyzing it. It was further separated into four fractions by gel filtration with Sepharose CL-4B, and they were designated Fractions B, C, D, and E. The results of the antitumor test showed that Fractions A, B, C, D and E had tumor inhibition ratios of 92.3, 78.5, 59.6, 77.4 and 62.2%, respectively. Anthrone test was carried out to determine the contents of total polysaccharide of the five fractions, and they had 46.3, 27.3, 65.3, 64.6, and 46.1%, respectively. The contents of the total protein of the five fractions were 29.4, 13.9, 14.3, 14.3, and 29.1%, respectively. Monosaccharide subunits of each fraction were analyzed with gas chromatography, and glucose, xylose, mannose, galactose and fucose were identified. Fraction A was examined for immunological effects. It increased the count of hemolytic plaque forming cells 12.8 times to that of the control group, and the population of macrophage in peritoneal cavity 3.2 times to that of the control group.

• Parasites, Hosts and Diseases
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• 제29권1호
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• pp.43-54
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• 1991

폐홉충(Paragonimus westermani) 초기 감염시의 숙주 면역 기전을 알아보기 위하여 폐흡충 피낭유충을 백서에 감염시켜 주별(주별)로 항혈청을 분리하고 등일 백서에서 복강 대식세포(homologous rat peritoneal macrophage)를 얻은 다음, 이들과 폐흡충 탈낭유충 및 숙주 조직 이행중인 유약충으로 effector system을 조작하여 폐흡충 유충에 대한 항체 의존성 대식세포 공격기전을 관찰하였다. 백서 복강 대식세포는 항혈청 출현 시에만 폐흡충 탈낭유충에 대한 세포부착 반응(cell adherence reaction)을 보였고 이 반응에는 보체가 관여하지 않았다. 폐흡충 감염 1주에서 8주까지의 백서 항혈청 중 2주째에 분리한 항쳔청 실험군에서 100%의 세포부착 반응을 나타내었고 사멸된 충체를 관찰할 수 있었으며, 세포부착 반응은 탈낭 유충기에서만 나타나는 반면 조직이행 유약충에서는 전혀 그 반응을 인정할 수 얼어 폐흡충 감염에 따른 항체의존성 숙주 세포 매개독성(antibodydependent cell·mediated cytotoxicity)은 발육단계 별 특이성을 보였다. 한편, 대식세포의 공격에 의하여 사멸된 폐흡충 탈낭유충의 형태학적 특징은 대식세포와 유충 사이에 형성되는 fuzzy material, tegumental syncytium 내의 미세구조의 변성 및 syncytium과 근육층을 연결하는 tubular tunnel의 형성 등이었다.

• Biomolecules & Therapeutics
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• 제18권3호
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• pp.308-315
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• 2010

Essential oil-excluded Artemisia princeps Pamp var Ssajuarissuk (AP) was fermented with Lactobacillus brevis K-1, which was isolated from cabbage Kimchi, and the anti-inflammatory effects of AP and fermented AP (FAP) on lipopolysaccharide (LPS)-induced inflammatory response in peritoneal macrophages were investigated. AP and FAP inhibited LPS-induced TNF-$\alpha$, IL-$1{\beta}$, COX-2, iNOS and COX-2 expression, as well as NF-${\kappa}B$ activation. AP and FAP also reduced ear thickness, inflammatory cytokine (TNF-$\alpha$, IL-$1{\beta}$ and IL-6) expression and NF-${\kappa}B$ activation with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced dermatitis in mice. Furthermore, AP and FAP also reduced exudate volume, cell number, protein amount, inflammatory cytokines (TNF-$\alpha$, IL-$1{\beta}$ and IL-6) expression and NF-${\kappa}B$ activation in carrageenan-induced air pouch inflammation in mice. The inhibitory effects of FAP were more potent than those of non-fermented AP. Based on these findings, we propose that FAP can improve inflammatory diseases, such as dermatitis, by inhibiting the NF-${\kappa}B$ pathway.

• Journal of Acupuncture Research
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• 제23권6호
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• pp.45-60
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• 2006

Objectives : This study was guided to estimate inhibitory effects against hepatic metastasis and activation of immunocytes by Carthami Semen Herbal Acupuncture. Methods : Colon26-L5 carcinoma cells were injected through hepatic portal vein to induce hepatic metastatic cancer. Changes in weight, morphology of the cancer were estimated and cytokine level was analyzed to produce immunological changes. Results: The experimental group was significantly decreased metastatic size and proliferation compared with the control group. Observing immunocytes from the spleen of experimental group T-lymphocytes were significantly increased. As compared with control group, the differentiation of total T cell, helper T cell, cytotoxic T cell, Macrophage and NK cell was increased. Measuring the level of cytokine $IFN-{\gamma}$ which stimulates Th 1 was significantly increased in splenocyte with Con A stimuli. $IFN-{\gamma}$ and IL-12 were significantly increased in peritoneal exudate macrophage with LPS stimuli. Conclusion : These findings strongly indicate Carthami Semen Herbal Acupuncture improves immunity to inhibit the growth of cancer and metastasis.

• 생약학회지
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• 제19권3호
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• pp.193-200
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• 1988

Immunopotentiating activity of water extract of Lycii Fructus, Rubus Fructus, Torilis Fructus, Schizandrae Fructus and Cuscutae Semen were investigated using mouse fed with normal diet and protein deficient diet. Body weight, ratio of spleen or thymus to body weight, number of total peritoneal exudate cell, content of total serum protein and albumin content were decreased in the mouse fed with protein deficient diets as compared to the mouse fed with normal diet. Regardless of the nutritional condition, the supplement of the water extract of Lycii, Rubus Torilis, Schizandrae Fructus or Cuscutae Semen increased the ratio of spleen to body weight. The increment of this ratio was significant by the supplement of water extracts of Lycii and Torilis Fructus in mouse fed with protein deficient diet. The supplement of water extracts of Torilis Fructus to the mouse fed with protein deficient diet increased the content of total serum protein and albumin content significantly. Similar results were obtained with Lycii, Rubus, Schizandrae Fructus and Cuscutae Similar. The electrophorogram shows the difference in the concentration of some serum protein constituents between the mouse fed with normal diet and protein deficient diet.

• KSBB Journal
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• 제6권1호
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• pp.45-54
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• 1991

The aims of this study were investigated the antitumor effects and immunological activities of lipopolysaccharides (LPS) extracted from Proteus vulgaris RH-90 toward sarcoma-180 cells. LPS extracted from Proteus vulgris RH-90 was irradiated with gamma ray for detoxification. The tumor incidence of sarcoma-180 occurs all group which injected with gamma ray irradiated LPS and tumor of sarcoma-180 was necrotized with breeding in the injected group of l0$\mu\textrm{g}$ LPS. The inhibition ratio of tumor growth showed at the highest level of 60.88% when 5$\mu\textrm{g}$ gamma ray irradiated LPS was injected into mice. The prolongation ratio of life showed 20.72% when injected into mice with gamma ray irradiated LPS of 5$\mu\textrm{g}$. In the effect of immunological activity, the number of circurating leucocyte and peritoneal exudate cells were increased significantly in the treatment group than that control group, and dose-dependent response indicated by the increase of weights of immunorgans which revealed the improvement of immunity. The effect of macrophage on phagocytes, there were not found the differences between phagocytic and corrected phagocytic index.

• 한국식품영양학회지
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• 제9권1호
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• pp.69-75
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• 1996

In order to research for compound of immunomodulatic and anti-allergic function. These experiments were conducted to investigated the effects of hot water extracts(PRWE) , ethanol extracts (PREE) and polysaccharide fraction extracts (PRPE) extracted from platycodi radix on immune response. The effect of these platycodi radix extracts on hemagglutinin titer(HA), hemalysin titer(HY), plaque forming cell(PFC), rosette forming cell (RFC) and phagocytosis was Investigated by using BALB / C mice. The results obtained from this study are as follows. Generally, the oral administration of extracts fractions for 10 days each other resulted in the enhanced HA and HY. In the experiment of PFC and RFC, the results of experimental groups which was given each samples compared to control group showed the enhanced level of activity such as PRPE 160% and 196% each other But PRWE and PREE decreased or wear not changed. When PREE, PRPE or PRWE was given to mice orally, PREE and PRPE significantly enhanced the phagocytic activity of peritoneal exudate cells(PEC), spleen cells(SC) and monolymphocytus cell(MC), about from 150% to 250%, but PRW was decreased.

• 약학회지
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• 제45권1호
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• pp.64-70
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• 2001

Protein-polysaccharide fractions, PJ-3 and PJ-4, were prepared from mycelial culture filtrate of an insect-born fungus, Paecilomyces japonica DGUM 32001, and subjected to a flow cytometrical analysis for their vivo antitumor and immunomodulating activity in ICR mice. When i.p. injected once daily for semen days at 100 mg/kg, PJ-4 exerted a strong antitumor activity showing the growth inhibition ratio of 85.1% against i.p. implanted sarcoma 180 cells, while PJ-3 showed only a weak activity. Moreover, PJ-4 signiscantly increased the expression level of CD25 (IL-2R $\alpha$-chain) as well as forward scatter (FSC) values of splenic CD8$^{8}$ T cells. It is also noteworthy that PJ-4 strongly induced the peritoneal exudate cells in the same experiment. In an in vitro study, PJ-4 slightly inhibited the growth of sarcoma 180 cells at the concentration of 50$\mu$g/ml or higher. These results strongly suggest that PJ-4 might exert its antitumor activity through immunostimulation as well as direct inhibitory activity on the tumor cells.

• 한국식품영양과학회지
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• 제26권1호
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• pp.148-153
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• 1997

본 연구는 전보의 영지버섯으로부터 분리 배양한 균 사체를 알카리추출하여 얻은 다당체의 일반성분과 당분석 및 항암작용에 관한 연구에 이어, 이들과 ATCC의 마우스 유래 탐식 세포주 TIB 71 및 BALB/c 마우스의 복강내 대식세포를 이용하여 영지버섯 다당체가 면역계 전체의 기능을 조정하는 대식세포의 면역기능에 미치는 영향을 연구하였다. 그 결과를 요약하면 다음과 같다 1. 대식세포의 환성에 영향을 미치는 영지 버섯 다당체의 효과를 알아보기 위하여 세포의 유사분 열능을 측정한 결과, 다당체의 처리 농도가 0.5mg 실험군에서부터 유의한 증가를 나타내었다(p<0.001). 2. 영지버섯 다당체가 대식 세포 활성에 미치는 효과를 알아보기 위해 대식세포의 표면에 존재하는 IL-2 수용체를 anti-mouse IL-2-FITC 단크론항체를 이용하여 측정한 결과는 PSG 0.05mg 실험군을 제외한 실험군 모두에서 대조군 37.6% 보다 현저한 발현증가(p<0.001)를 보였으며 PSG 0.1mg 실험군까지는 수용체가 증가 하나 그 이상 처리군 사이에서는 상호간 유의한 차이는 없었다. 3. 영지버섯 다당체는 대식 세포에서 유래하는 세포간물질 즉 IL-1, IL-6 및 TNF의 생성에 유의성 있는 관여는 하지 않는 것으로 생각되나 부분적으로 영지버섯 다당체와 BCG및 IFN-${\gamma}$를 모두 함께 병용 투여함으로써 각각 영지버섯 다당체와 BCG또는 영지버섯 다당체와 IFN-r을 투여하는 경우에 비하여 IL-1의 분비증가를 보였으며 TNF의 분비 역시 현저한 증가를 보여 영지버섯 다당체가 초기 감염시 숙주의 1차 방어기능에 관여함을 알 수 있었다.