• 대한수의학회지
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• 제47권4호
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• pp.443-447
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• 2007

A 7-year-old, female snow goose (Anser caerulescens hyperboreus) with history of decreased activity for 2 month died in Daejeon Zoo Land in September 2006. At necropsy, granulomatous pneumonia and hepatomegaly with multiple cysts were observed. Small masses were found in the spleen. Microscopically, fibrinous pneumonia distributed in most of the lung lobe with pulmonary edema and congestion. Especially, granulomatous inflammation with numerous multinucleated giant cells was observed around the dilated bronchi. To confirm the diagnosis, acid-fast (Ziehl-Neelsen method) and periodic acid-Schiff (PAS) staining was performed. Acid-fast staining showed red bacterial colony indicating tuberculosis. PAS staining was also positive enough to diagnose aspergillus spp. co-infection that was an opportunistic fungi occurring in immuno-compromised animals. Based on the above results, we confirmed that the case submitted was diagnosed as avian tuberculosis.

• BMB Reports
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• 제32권3호
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• pp.317-319
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• 1999

A leucine aminopeptidase has been isolated from the culture medium of the soil fungus, Aspergillus flavus. The enzyme was found to be a glycoprotein, as judged by electrophoresis analysis and the subsequent staining by the periodic acid-Schiff's reagent. Carbohydrate moieties could be cleaved by N-glycosidase, but not by O-glycosidase, indicating that the glucans are linked to the asparagine residue in the protein. Removal of N-glucans was observed without prior denaturation of the protein, implying that the N-glycosidic linkage is exposed and accessible to glycosidase. When the activity of native or deglycosylated enzyme was measured in the presence of various metal ions, removal of carbohydrates increased the aminopeptidase activity of the enzyme.

• 한국임상수의학회지
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• 제18권2호
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• pp.174-177
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• 2001

A nine-month-old male ostrich (Struthio camelus) exhibited loss of appetite and dyspnea for 10 days, followed by emaciation and death. Grossly, multiple white nodules measuring 1-3 mm in diameter were observed in the surface of and inside of the lung and in the mucosa of the air sac. Microscopically, the granuloma formations were observed in the lung and air sac. The core of granuloma consisted mainly of macrophages and fibroblasts. The thin layer comprising the giant cells and macrophages surrounded the granuloma. By Periodic acid Schiff reaction, mycelia were detected especially in the core of granuloma. From the present results, the causative agent inducing the death of an ostrich was thought to be Aspergillus sp. This is the first report of the occurrence of acute Aspergillus pneumonia (brooder pneumonia) in an adult male ostrich in Korea.

• Parasites, Hosts and Diseases
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• 제40권1호
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• pp.55-58
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• 2002

The effects of exogenous glucose in artificial spring water (ASW) were studied on the survival and infectivity of Schistosoma mansoni cercariae. The mean percent survival of cercariae maintained in 1% glucose in ASW for 36 and 48hr was significantly greater than that of cercariae maintained identically in ASW. Cercariae maintained in ASW with or without glucose for 24hr, fixed in neutral buffered formalin, and stained in Oil Red O. showed an accumulation of neutral lipid in the tail. Cercariae maintained as described above and stained in periodic acid-Schiff exhibited depleted glycogen, mainly from the tail. Cercariae maintained in ASW with glucose for 24hr did not resynthesize glycogen. Cercariae maintained in ASW with glucose for 24hr were as capable of infecting male FVBN202 mice as were freshly emerged cercariae, and increased the percent of worm recovery. Exogeneous glucose added to ASW prolonged the survival of S. mansoni cercariae and increased infectivity in terms of worm recovery.

• 한국수의병리학회지
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• 제7권1호
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• pp.67-69
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• 2003

Three mature layer chickens from a farm in which chickens showed green diarrhea, cyanosis, lethargy, loss of appetite were pathologically examined. Grossly, multiple variable sized caseous nodules were detected in the liver, intestinal serosa and mesentery. In addition, parathypoid nodules in the liver and fibrous serositis on the several peritoneal organs and tissues were noticed. One of spleens had multiple infarction areas. Histologically caseous nodules consisted of central caseous core and peripheral epithelioid cells overlying the fibrous connective tissue. Multinucleated giant cells were scattered between the epithelioid cells and fibrous connective tissue. In these nodules Gram negative cocobacilus bacterial colonies were present, whereas Periodic Schiff reaction and Ziehl-Neelsen stain detected neither fungi nor acid fast bacteria. From these results multiple granulomas might be induced by Escherichia coli. In addition, severe Ascafdiodf and Salmonellosis were coinfected in these chickens.

• 한국동물위생학회지
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• 제35권1호
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• pp.73-76
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• 2012

A female slaty-backed gull (Larus schistisagus) died suddenly without apparent clinical signs. At necropsy, well demarcated 7 to 10 mm yellow to white nodules were presented at the lungs and thoracic cavity. Microscopically, multifocal necrotic granulomas were observed in the lung tissue and amorphous acidophilic fibrin were accumulated in the granuloma and normal alveolar space. Periodic acid-Schiff (PAS) staining demonstrated numerous pinkish red branched hyphae embedded in the center of these granulomas. According to fungal culture using Sabouraud's dextrose agar plate, Aspergillus fumigatus was isolated from lung lesions. This is the first report for pulmonary aspergillosis of wild slaty-backed gull in Korea.

• 대한치과의사협회지
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• 제9권6호
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• pp.293-296
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• 1971

Histochemical observations on the effect of gold alloys crown margin in the periodontal membrane were caried out by use of rabbits. The animal were setted with gold alloys crown on the incisor teeth. The cervical margins were given extence into gingival sockets. The animals were sacrificed at the 30 days experimented period. Specimens of tissue were obtained from the mandibular incisor teeth with jaw. All tissues were fixed immediately in 10% neutral formalin solution. Different histochemical staining methods for the determination of fibrous components were the Mallory-Heidenhain Bielschowsky-Gomori, Gomori's aldehyde fuchsin. Periodic acid-Schiff reaction and Hematoxylin eosin stanin method. The results were as follows. The periodontal membrane is composed primarily of collagenous fibers and fibrous component run functional arrangement but in lower gold content groups, horizontal fiber groups were irregularly run. Elastic fibers were obligue run in experimental groups. There were no significant differences in reticularfibers in distribution.

• 대한수의학회지
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• 제44권4호
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• pp.615-619
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• 2004

A three years old male Jackass penguin(Spheniscus demersus) showed anorexia, depression, respiratory problem for a few days, followed by clinical treatment for 20 days resulted in death. Grossly, multiple white nodules measuring 2~3 mm in diameter were observed in the surface of and inside lung, liver, kidney, thoracic wall. Microscopically, granuloma formations were observed in the lung and liver. The margin of granuloma surrounded by connective tissue barrier and infiltrated lymphocytes, and also observed giant cell near the granuloma. By Periodic acid Schiff reaction, hyphae were detected in granuloma of lung and liver. This case was a chronic and systemic aspergillosis caused by Aspergillus fumigatus infection in a Jackass penguin at a zoo.

• 대한수의학회지
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• 제59권1호
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• pp.47-49
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• 2019

A renal oncocytoma was diagnosed in an 8-year-old female Maltese dog with a history of renal cysts. Tumor cells were not detected until six months after observation of renal cysts. Nephrectomy was performed to treat the neoplasia. Tumor-like masses with numerous nodules were observed in the inner surface of cysts present in the caudal part of the left kidney. Histologically, the tumor consisted of cells with abundant eosinophilic cytoplasm. The diagnosis was based on histological features, periodic acid-Schiff reaction, and immunohistochemical cytokeratin staining. Based on a literature review, this is the first canine renal oncocytoma case reported in Korea.

• 한국임상수의학회지
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• 제36권3호
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• pp.172-175
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• 2019

A young slaty-backed gull was found dead nearby Seongsan harbor, Seogwipo-si, Jeju. Grossly, focal ulceration and a protuberant nodule were observed in intermediated zone between proventriculus and gizzard. Numerous discrete or confluent white-yellowish nodules were scattered on the surface of liver. Caudal portion of left kidney was replaced by a white-yellowish nodule. Three raised masses were observed in the spleen. Histologically, there were severe ulceration with hemorrhage in the gizzard and multifocal granulomatous inflammations in liver, kidney, spleen, and lungs. Pink to dark-red, round to oval fungal spores and pseudohyphae in granulomatous lesions were confirmed by periodic acid-Schiff stain. Candida albicans were isolated from the lesions of liver, kidney, spleen and gizzard using fungal culture and confirmed by VITEK 2 system. Based on the gross, histopathological findings, and fungal examination, this case was diagnosed as systemic candidiasis of a wild slaty-backed gull. In our best knowledge, this is the first report for systemic candidiasis characterized by multifocal granulomatous lesions in internal organs of a wild gull in Korea.