• 한국임상수의학회지
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• 제33권4호
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• pp.218-220
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• 2016

A 2-year old female African hedgehog (Atelerix albiventris) weighing 645 grams was presented with a well circumscribed mass in the subcutaneous of the left shoulder. It measured $2{\times}2cm$ in size and had a hard texture. There were no clinical signs. In a fine-needle aspiration (FNA) cytological evaluation, mast cells were identified with Wright-Giemsa stain. Mast cell tumor was confirmed and characterized by surgical sampling and histopathological evaluation. Four stains (hematoxylin-eosin, toluidine blue, periodic acid-Schiff, and Safranin O) were examined in order to find an applicable stain for a mast cell tumor histopathological evaluation in hedgehog. This case showed that periodic acid-Schiff and toluidine blue staining were the most useful for assessment of a mast cell tumor in a hedgehog.

• 대한치과의사협회지
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• 제10권4호
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• pp.209-212
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• 1972

The effect of pyridoxine on the incisors were histochemically studied for the purpose of identifying the phosphoric acid. The microscopic observations were carried out by means of phosphoric acid reaction(Serraet Queiroz-Lopes method), mucoprotein reaction (Clara method), periodic acid-Schiff reaction (McManus method), methylen blue stain and hematoxylin-eosin stain. In the experimental result, it was found that phosphoric acid reactions of ameloblast, Tomes' process and preenamel were remarkably increased after pyridoxine administration.

• 대한수의학회지
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• 제32권4호
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• pp.463-479
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• 1992

The morphological findings of the parotid, mandibualr and sublingual salivary glands of the Korean native goat have been investigated by the histological and histochemical observation using the light microscope. Tissues were fixed with 10% neutral buffered formalin and Bouin's solution, and embedded in paraffin. The tissue sections were stained with hematoxylin and eosin, Heidenhain's azocarmine-aniline blue, alcian blue, toluidine blue, periodic acid Schiff, aldehyde fuchsin, alcian blue-periodic acid Schiff and aldehyde fuchsin-alcian blue. Some sections were stained with the alcian blue after each teatment of diastase digestion, methylation, methylation-saponification, and neuraminidase digestion. The results were as follows ; 1. The major salivary glands were compound. tubuloacinar glands, and the parenchyma was composed of acini, intercalated ducts, striated ducts and excretory ducts. 2. The acini were composed of serous cells in the parotid gland, and mucous cells, serous cells and seous demilunes in the mandibular gland. The acini of the sublingual glands were composed of mucous cells and serous demilunes. 3. In histochemistry, the serous cells of the parotid gland contained neutral mucin and enzyme-liable silaic acid. 4. The serous cells and demilunes of the mandibular gland contained neutral mucin and enzyme-liable sialic acid, and the mueous cells contained sulfated mucin, enzyme liable sialic acid and neutral mucin 5. In the sublingual gland, the mucous cells contained sulfated mucin, enzyme-resistant sialic acid and neutral mucin, and the serous demilunes contained neutral mucin and enzyme-resistant sialic acid.

• 한국식품과학회지
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• 제14권4호
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• pp.336-341
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• 1982

미역김의 제조와 이화학적 특성에 관한 계속 연구로 실시한 미역김의 조직화학적 특성 연구 결과는 다음과 같다. 미역잎은 표층, 피층, 속층으로 구성되어 있는데 미역김의 결합단면을 광학현미경으로 관찰한 결과 피층과 속층이 완전히 파괴되었고 표층세포끼리 결합되어 있었다. 미역김 절편을 $Na_2CO_3$용액 처리로 알진산을 추출한 후 광학현미경으로 검경한 결과 알진산이 추출된 결합부위는 periodic acid schiff reagent반응이 나타나지 않았으며, 한편 미역잎을 $Na_2CO_3$용액으로 미리 처리하여 알진산을 추출한 후에는 정상적인 미역김을 제조할 수 없었고 비정상적인 결합구조이었으므로 알진산이 미역김의 결합물질임을 확인할 수 있었다. 미역김의 결합부위를 전자현미경으로 관찰한 결과 알진산의 결합위치는 미역김의 표층세포막과 세포막사이임이 밝혀졌다. $Na_2CO_3$용액을 처리한 미역잎으로 제조한 비정상적인 미역김의 결합구조는 표층세포껍질로 인지되는 물질만이 서로 엉켜 있었다.

• 대한수의학회지
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• 제36권3호
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• pp.521-529
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• 1996

In an effort to obtain basic data of carbohydrate metabolism during spermiogenesis of the sexually-matured Jindo dog, the glycogen distribution in the testis was investigated by light and transmission electron microscopy. Periodic acid thiocarbohydrazide silver proteinate physical development(PA-TCH-SP-PD) staining method provided better results in the detection of glycogen granules from Sertoli cells and germ cells than the periodic acid schiff(PAS) staining method did. Pre-treatment of the tissue sections with ${\alpha}$-amylase elicited a significant decrease in PA-TCH-SP-PD stained granules, which suggested that the stained granules were of glycogen origin. High concentration of the glycogen granules were observed in the Sertoli cells, especially in its column, sheet-like processes, club-like processes, and tubular processes. The glycogen granules were unevenly distributed in some Sertoli cell columns. These results strongly indicated that the Sertoli cells of Jindo dogs showed vigorous activity of carbohydrate metabolism.

• Applied Microscopy
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• 제51권
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• pp.5.1-5.7
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• 2021

The olfactory organs of two African lungfishes, Protopterus amphibius and P. dolloi, were investigated using a stereo microscope and a compound light microscope and were described anatomically, histologically, and histochemically. Like other lungfishes, these species present the following general features: i) elongated olfactory chamber (OC), ii) anterior nostril at the ventral tip of the upper lip, iii) posterior nostril on the palate of the oral cavity, iv) lamellae with multiple cell types such as olfactory receptor neurons, supporting cells, basal cells, lymphatic cells, and mucous cells (MC), and vi) vomero-like epithelial crypt (VEC) made of glandular epithelium (GE) and crypt sensory epithelium. Some of these features exhibit differences between species: MCs are abundant in both the lamellar and inner walls of the OC in P. amphibius but occur only in lamellae in P. dolloi. On the other hand, some between feature differences are consistent across species: the GE of both P. amphibius and P. dolloi is strongly positive for Alcian blue (pH 2.5)-periodic acid Schiff (deep violet coloration), and positive with hematoxylin and eosin and with Masson's trichrome (reddish-brown staining), unlike the MCs of the two species which stain dark red with both Alcian blue (pH 2.5)-periodic acid Schiff and Masson's trichrome but respond faintly to hematoxylin and eosin. The differing abundance of MCs in the two lungfishes might reflect different degrees in aerial exposure of the olfactory organ, while the neutral and acid mucopolysaccharide-containing VEC, as indicated by staining properties of the MCs, is evolutionary evidence that P. amphibius and P. dolloi are the closest living relatives to tetrapods, at least in the order Dipnoi.

• 디지털융복합연구
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• 제12권6호
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• pp.433-438
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• 2014

본 연구는 흰쥐의 정소에 방사선을 조사하였을 때 시간이 경과함에 따라 나타나는 정소세포 및 정자의 변화를 현미경학적으로 얻은 영상을 기초로 영상처리에 의한 정량분석을 통하여 정자의 변화에 대한 결과를 정량화함으로써 더 정확하고 객관적인 결과를 얻고자 하는데 그 목적을 두고 있다. 연구에는 8주 수령의 흰쥐를 대상으로 하였으며, 6 MV의 X선을 1회 2 Gy를 전신 조사하였다. 총 30 마리의 흰쥐를 대상으로 방사선을 조사하지 않은 정상대조군과 실험군을 조사 즉시, 조사 2시간, 4시간, 8시간, 24시간 후에 각 실험군 마다 5마리의 정소를 각각 적출하였다. 적출 후 Periodic acid Schiff 염색을 시행하여 정상대조군과 실험군에서의 정소세포와 정자의 상태를 관찰하였다. 방사선조사 후 24시간까지, 정소세포수와 정자수가 점차 감소하는 것을 정성적 및 정량적으로 유의함을 확인하였다.

• 환경생물
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• 제22권3호
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• pp.387-393
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• 2004

사료를 통한 benzo(a)pyrene이 조피볼락, Sebastes schlegeli의 lipid peroxidation (LPO), superoxide dismutase(SOD)활성과 간 조직의 병변 현상에 미치는 영향을 연구하기위해 0 (대조구), 0.5, 1.0, 1.5, 2.0 mg $kg^{-1}$ 농도의 사료를 조제하여 30일간 급이하였다. LPO는 2.0mg kg$^{-1}$농도구에서 전 실험기간 동안 유의적으로 증가하였고, SOD 활성도 2.0 mg $kg^{-1}$ 농도구에서 30일째에 유의적으로 증가하였다. 간 조직 검경시 10일째에 1.0mg $kg^{-1}$ 이상의 농도구에서 세포비대가 나타났고, 같은 농도에서 periodic acid-Schiff (PAS) 염색시 양성으로 나타나는 granule이 30일째에 나타났고, 2.0mg $kg^{-1}$ 농도구에서는 20일째부터 관찰되었다. 그리고, 30일째 2.0mg $kg^{-1}$ 농도구의 일부 어체에서는 간 세포의 괴사가 관찰되었다.

• 대한족부족관절학회지
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• 제4권1호
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• pp.30-34
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• 2000

The Actinomyces organism is inherent in human as normal flora found in the mouth. Outside of the mouth, this organism can cause actinomycosis. Involvement of the extremity is very rare and atypical. The authors have experienced a case of actinomycosis of the foot and report with the literature review. Histopathologic finding shows multiple sulfur granules. In histochemical stains, periodic acid-Schiff, Gomori methionine silver, and gram stain were positive, and acid fast bacilli stain was negative. The patient was treated with surgical excision and antibiotic administration with Ampicillin for 6 months.

• 한국식품과학회지
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• 제31권1호
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• pp.62-67
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• 1999

과요오드산 산화 가용성전분 및 말토올리고당을 고구마 ${\beta}-amylase$, 밀 ${\beta}-amylase$, aldolase, bovine serum albumin, catalase, carboxypeptidase, ferritin, pronase와 반응시켜서 전기영동하였다. 이들 단백질은 전기영동상의 이동도가 달라지고, 단백질 밴드와 같은 위치에서 PAS 염색 밴드를 나타내어 당이 부가된 것으로 확인되었다. 당의 부가는 과요오드산 산화당의 알데히드기가 단백질 분자 표면 리신의 ${\varepsilon}-NH_2$기와 Schiff 염기를 형성하여 일어난다. 변형효소는 자외흡광곡선에 변화를 나타내지 않았다.