• Journal of Environmental Science International
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• v.16 no.12
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• pp.1425-1430
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• 2007

This study was conducted to investigate the effect of ozonated water and ozonated water+hydrogen peroxide treatment of residual procymidone in perilla leaf containing 20 mg/L procymidone. Samples was treated with ozonated water containing 1.0, 2.0 and 3.0 mg $O_3/L$ ozone and hydrogen peroxide water containing 1.0, 2.0 and 3.0 mg $H_2O_2/L$ hydrogen peroxide in pH 5, 7 and 9, respectively, at $15^{\circ}C$. Procymidone removal rate was 26.5% in 7 days at $15^{\circ}C$ and optimum condition of procymidone removal was the case of treating with ozonated water containing 2.0 mg $O_3/L$ and pH 9. As the result procymidone removal rate was about 96.5%. In this case of adding hydrogen peroxide, optimum condition of procymidone removal was $1:0.5{\sim}1(O_3:H_2O_2)$. However, procymidone was nearly removed with the treatment of hydrogen peroxide water only.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.975-979
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• 2003

Perilla leaves cultivated in greenhouses (Jan., May) and in the fields (Aug.) of Geumsan province were investigated for their extract yields and physiological functionalities. The yield was highest in 30% ethanol extracts of the August perilla leaves. The highest fibrinolytic activity (8.2 U) was observed in 30% ethanol extracts of the May perilla leaves, while the HMG-CoA reductase inhibition level, which is related to the inhibition of cholesterol biosynthesis, was 83% in water extracts of the August perilla leaves. Anti-hypertensive ACE inhibitory activity was 64.5% in the water extracts of the January perilla leaves, and antioxidative electron donating ability was the highest (69%) in 30% ethanol extracts of the August perilla leaves. Elastase inhibitory activity, which is related to the inhibition of skin aging, was highest (47.5%) in 30% ethanol extracts of the May perilla leaves. However, SOD-like activity, nitrite scavenging activity, and tyrosinase inhibitory activity were not detected were very weak in all samples.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.134-141
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• 2011

Two-hundred Bacillus cereus isolated from perilla leaf cultivation areas in Miryang, Korea were investigated for toxin genes and antibiotic susceptibility. Toxigenic patterns of isolates were identified to be 11 groups through toxin gene profiles. 21% of strains isolated from the perilla leaves had both enterotoxin and emetic toxin. Toxin genes entFM (100%), nheA (100%) and hblA, C, D (65.5%) were frequently found in the perilla leaves, whereas EM (21.0%) was less common. Most isolates were susceptible to 10 antibiotics, but they were highly resistant to penicillin (100%), ampicillin (100%), oxacillin (94.9%), amoxicillin-clavulanic acid (95.6%), cefazolin (78.2%), and rifampicin (58.0%). These results indicate that food-borne outbreak caused by B. cereus might lead to diarrhea and emetic syndromes.

• Korean Journal of Plant Resources
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• v.7 no.2
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• pp.109-114
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• 1994

Followings are resulted through the analysis of the maln characters and the leaf ingredients, which is on the purpose of getting the basic materials to improve jaso and increase its production.Of the introduced varieties, Jukyeubjaso-stem length is 180cm, ear length is 37cm, No. of branches ofstem is 27, leaf length Is 16cm, leaf width Is 15cm-has better character than any other. Gurye local-stem length is 179cm, ear length is 38cm-is taken to be the best in the Geo-ecotypic.In the yield of leaf and seed, JukyeubJaso Is 727kg/10a, the greatest of all. In the leaf softness, allthe Inirorluced varieties are about 3.5 degrees, the Geo-ecotypic, about 2. The qualify of the introducedvat'ieties is better· than that of the Geo-ecotypic. Estimating on the chemical analysis of leaf,Jukyeubjaso has Protein 21.84%, Total carbohydrate 7.91%, Fe 126ppm, and Gurye lacal has Protein21.7 a, Total carbohydrate 8.4%, Fe 148ppm, expressing the highest. In correlation coefficients of leafchemical components, between protein and Total carbohydrate, Protein and Mn, protein and Fe, Pro-tein and Cu all the sorts show the positive correlation. Among them the Geo-ccoyplcs show the hlghestsignificance.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.243-248
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• 2011

The prevalence and distribution of hazardous microorganisms were investigated from the major perilla cultivation area at Milyang, Gyeongnam province, Korea. Aerobic plate count (APC) and coliform count of perilla leaves were 4.82 log CFU/g and 3.85 log CFU/g, respectively. E. coli, S. aureus and B. cereus were detected in 3.0% (4/114), 7.9% (9/114) and 46.5% (53/114) of examined perilla leaves. However, E. coli O157:H7, Salmonella spp, and L. monocytogenes were not detected. The distribution of hazardous microorganisms in perilla leaf cultivation environment were compared and the concentration of APC and coliform counts were more than 3.0 log CFU/(mL, g, $100cm^2$, hand) from most of the samples. S. aureus were detected from irrigation water, packing table, packing vinyl, hand, and clothes. Also, B. cereus was frequently detected from the examined samples. Especially, packing table and collection container were contaminated with maximum 5.5 log $CFU/100cm^2$ of B. cereus. Good Agricultural Practice (GAP) system should be introduced to farms to enhance the safety of perilla leaves.

• Korean Journal of Environmental Biology
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• v.39 no.2
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• pp.147-155
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• 2021

To investigate the incidence of plant-parasitic nematodes (PPNs) in perilla fields in Korea, 55 soil samples were collected from open fields and plastic-film house fields and were analyzed during January 2020 to October 2020. Root-lesion nematodes (RLNs), spiral nematodes, root-knot nematodes, and stunt nematodes were detected in perilla fields, and the incidences of RLNs (39%) and spiral nematodes (55%) were higher than those of other nematodes. Among PPNs, RLNs are very important species found on economic crops. The detection frequency of RLNs was increased due to continuous crop cultivation, and the frequency of fields that cultivated only one crop continuously for over 11 years was twice higher than the frequency of fields that cultivated only one crop for less than 5 years. The PPN species diversity and density were different between leaf-perilla cultivation fields and seed-perilla cultivation fields. In phylogenetic analysis of RLNs, Pratylenchus penetrans, P. vulnus, and Pratylenchoides leiocauda were identified in perilla fields. These results suggest the RLNs should be considered for establishing nematode management strategies in perilla fields in Korea.

• Food Science and Preservation
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• v.24 no.2
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• pp.312-319
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• 2017

In the present study, disinfection efficacy of slightly acidic electrolyzed water [SlAEW, 30 ppm of effective chlorine at $20{\pm}1^{\circ}C$, oxidation-reduction potential (ORP) $562{\pm}23mV$, pH 6.4] on 4 kinds of vegetables (lettuce leaf, endive leaf, perilla leaf and kale leaf) was evaluated to obtain a microbial reduction characteristics which are necessary to design a process control for non-thermal sterilization of fresh vegetables. Active chlorine, residual chlorine, microbial counts and residual microbial counts, which are the key factors in the non-thermal sterilization process were measured by dipping them in SlAEW three times for 30 minutes in order to analyze the relationship between factors. Total microbial count was decreased mostly during the first 10 minutes of washing, and the limit value that can be reduced by immersion treatment was 3 log CFU/g for the total microbial count surviving in 4 kinds of vegetables. The total number of microorganism that can be reduced by washing in SIAEW for 10 min was found to be about 2 log CFU/g on average. In addition, the active chlorine decreased in the initial 10 minutes in 2.2 ppm, 2.0 ppm, 1.7 ppm and 2.5 ppm in lettuce, perilla leaf, endive leaf and kale leaf, respectively, and about 50-80% of the chlorine was reduced in the initial 10 min appear.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.408-415
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• 2013

To better understand the morphological variation of the Perilla crop and their weedy types in East and Southeast Asia, we studied the morphological variation of 90 accessions by examining 10 morphological characteristics, such as flowering time, seed size, seed hardness, seed color, color of surface leaf, color of reverse side leaf etc. As a result, morphological variation determined that between cultivated var. frutescens and var. crispa, and between cultivated var. frutescens and its weedy type showed significant morphological differences in terms of seed size and seed hardness, whenever cultivated var. crispa and its weedy type could not showed significant differences in most morphological characters. In PCAs (principal component analysis), among 10 morphological characteristics, flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), seed color (QL2), stem color (QL7), and color of reverse side leaf (QL4) contributed in negative direction on the first axis, while flowering time (QN1), leaf shape (QL5), and degree of pubescence (QL8) contributed in positive direction on the first axis. Among these morphological characters, particularly flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), and degree of pubescence (QL8) were useful characters for discrimination between cultivated var. frutescens and weedy var. crispa, and between cultivated var. frutescens and its weedy type. However, most accession of cultivated and weedy types of var. crispa was not clearly discriminated by PCA analyses. Although the wild ancestral species of var. frutescens and of var. crispa are still unknown in East and Southeast Asia, the weedy types of Perilla crop may be the key taxon for our understanding of the origin of cultivated types of var. frutescens and var. crispa.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.221-227
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• 2016

This study was conducted to investigate residual organochlorine pesticides in green house soil and green perilla leaves. Extraction and clean-up method were developed using the modified QuEChERS method for residual organochlorine pesticides (ROCPs) in soil and green perilla leaves. Recovery and limit of quantitation (LOQ) of ROCPs in greenhouse soil and green perilla leaves were 76.3-113.4 and 79.4-107.3%, 0.03-0.24 and $0.33-0.50{\mu}g/kg$, respectively. Detected ROCPs in greenhouse soil were dieldrin and endosulfan sulfate, the residue were 1.6-9.2 and $22.0-87.8{\mu}g/kg$, respectively. But two pesticides in all green perilla leaf samples were not detected. These results showed that ROCPs residue in greenhouse soil was lower than the level of bioaccumulation occurring.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.48-54
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• 1997

This study was conducted to determine the effect of temperatures and food sources on the egg and larval developmentof the tobacco cutworm, Spodoptera litura Fabricius. The hatchability of egg masses of S. liturawas 100% on the leaf of soybean, perilla and sweet potato in any given temperature regimes, while the hatchabilitywas only 65-8796 when reared on the pulp paper and decreased as temperature increased. Egg durationwas not significantly different among different food sources within each temperature. However, egg duration at32$^{\circ}$C was shorter than that at 24$^{\circ}$C and 28$^{\circ}$C. During the early larval development, at 28$^{\circ}$C and 32$^{\circ}$C the larvafed on sweet potato leaf was heavier than those fed on soybean and perilla leaves and the opposite case wastrue during mid-larval development stage. However, larval weight at 24$^{\circ}$C was heavier on sweet potato leafthan that on soybean and perilla leaves until 12 days after hatching. This result was probably due to relativelyslower developmental rate at 24$^{\circ}$C compared to 28$^{\circ}$C and 32$^{\circ}$C. The mean larval mortality was 68.896, 44.5%and 33.8% at 24$^{\circ}$C. 28$^{\circ}$C and 32"C, respectively. The lowest mortality was observed on soybena leaf and followedby perilla and sweet potato leaves, and artificial diet regardless of temperature conditions. The durationwas the shortest when they fed on soybean leaf, and followed by perilla and sweet potato leaves and artificialdiet. Larval durations were 23.6-30.4 days at 24$^{\circ}$C. 18.6-22.3 days at 28$^{\circ}$C and 14.5-18.0 days at 32$^{\circ}$C. Thethreshold temperatures of egg and larva of S. litura were about 6.l"C and 10.9"C, respectively.t 6.l"C and 10.9"C, respectively.pectively.