Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Profiles of Toxin Genes and Antibiotic Susceptibility of Bacillus cereus Isolated from Perilla Leaf and Cultivation Areas

들깻잎과 생산환경에서 분리한 Bacillus cereus의 독소 유전자와 항생제 감수성 분석

  • Kim, Se-Ri (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Lee, Ji-Young (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Lee, Seo-Hyun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Ryu, Kyoung-Yul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Kyeong-Hun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Kim, Byung-Seok (Administrative Management & Legal Affairs Office, RDA) ;
  • Yoon, Yo-Han (Department of Food & Nutrition, Sookmyung Women's University) ;
  • Shim, Won-Bo (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Kyoung-Yul (Division of Applied Life Science, Gyeongsang National University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Yun, Jong-Chul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 이지영 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 류경열 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 박경훈 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 김병석 (농촌진흥청 기획조정관 행정법무담당관실) ;
  • 윤요한 (숙명여자대학교 생활과학부 식품영양학과) ;
  • 심원보 (경상대학교 응용생명과학부) ;
  • 김경열 (경상대학교 응용생명과학부) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 윤종철 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Received : 2010.09.27
  • Accepted : 2010.12.14
  • Published : 2011.04.30
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Abstract

Two-hundred Bacillus cereus isolated from perilla leaf cultivation areas in Miryang, Korea were investigated for toxin genes and antibiotic susceptibility. Toxigenic patterns of isolates were identified to be 11 groups through toxin gene profiles. 21% of strains isolated from the perilla leaves had both enterotoxin and emetic toxin. Toxin genes entFM (100%), nheA (100%) and hblA, C, D (65.5%) were frequently found in the perilla leaves, whereas EM (21.0%) was less common. Most isolates were susceptible to 10 antibiotics, but they were highly resistant to penicillin (100%), ampicillin (100%), oxacillin (94.9%), amoxicillin-clavulanic acid (95.6%), cefazolin (78.2%), and rifampicin (58.0%). These results indicate that food-borne outbreak caused by B. cereus might lead to diarrhea and emetic syndromes.

본 연구는 들깻잎과 들깻잎 생산환경을 대상으로 276개의 시료를 채취하여 B. cereus를 분리 하였다. 분리된 B. cereus 200주의 toxicity를 평가하고자 독소유전자와 항생제내성을 검색하였다. 그 결과 분리된 균주에서 11개의 서로 다른 독소유전자 패턴은 확인하였으며 5개의 설사형독소와 구토형 독소를 모두 생성할 수 있는 균주는 21%였다. 가장 빈번하게 검출되는 독소유전자는 nheA(100%), enFM(100%), hblA, C, D(66.5%)였으며 EM은 가장 낮은 빈도(21.0%)로 검출되었다. 항생제 내성평가결과 분리된 대부분의 B. cereus는 18종의 항생제 중 10개의 항생제에 대해서는 감수성이었으나 ${\beta}$-lactam계 항생제인 penicillin(100%), ampicillin(100%), oxacillin(94.9%), amoxicillin-clavulanic acid(95.6%), cefazolin(78.2%)과 비${\beta}$-lactam계 항생제 rifampicin(58.0%)에 대해서 저항성을 보이는 것으로 나타났다. 들깻잎과 들깻잎 생산환경에서 분리된 B. cereus의 독소유전자와 항생제내성 패턴은 서로 유사하였다. 따라서 본 연구결과는 들깻잎에 오염된 B. cereus에 의하여 설사형 뿐만 아니라 구토형 식중독이 발생할 가능성을 시사하며 들깻잎과 생산환경에서 항생제 저항성 B. cereus가 검출되어 의약계뿐만 아니라 농업현장에서도 항생제내성균주 출현을 예방하는 대책이 요구된다.

Keywords

References

  1. Sevell AM, Farber JM. Foodborne outbreaks in Canada linked to produce. J. Food Protect. 64: 1863-1877 (2001)
  2. Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim KS, Ha SD. Microbial contamination levels of fresh vegetables distributed in markets. Korean J. Fd. Hyg. Safety 20: 43-47 (2005)
  3. FDA. Guidance for industry, Guide to minimize microbial food safety hazard for fresh fruits and vegetables. Available From: http://csan.fda.gov. Accessed Oct. 26, 2005.
  4. Burnett SL, Beuchat LR. Human pathogens associated with raw produce and unpasteurized juices and difficulties in decontamination. J. Ind Microbiol. Biot. 27: 104-110 (2001) https://doi.org/10.1038/sj.jim.7000199
  5. Kim SH, Kim JS, Choi JP, Park JH. Prevalence and frequency of food-borne pathogens on unprocessed agricultural and marine products. Korean J. Food Sci. Technol. 38: 594-598 (2006)
  6. Choo EY, Jang SS, Kim KS, Lee KG, Heu SG, Ryu SR. Prevalence and genetic diversity of Bacillus cereus in dried red pepper in Korea. J. Food Protect. 70: 917-922 (2007)
  7. Kim HJ, Lee DS, Paik HD. Characterization of Bacillus cereus isolated from raw soybean sprouts. J. Food Protect. 67: 1031-1035 (2004)
  8. Hansen BM, Laser TD, Hendriksen NB. Polymerase chain reaction assay for the detection of Bacillus cereus group cells. FEMS Microbiol. Lett. 202: 209-213 (2001) https://doi.org/10.1111/j.1574-6968.2001.tb10805.x
  9. Anderson Borge GI, Skeie M, Sorhaug T, Langsrud T, Granum PE. Growth and toxin profiles of Bacillus cereus isolated from different food sources. Int. J. Food Microbiol. 69: 237-246 (2001) https://doi.org/10.1016/S0168-1605(01)00500-1
  10. Phelps RJ, McKillip JL. Enterotoxin production in natural isolates of Bacillaceae outside the Bacillus cereus group. Appl. Environ. Microb. 68: 3147-3151 (2002) https://doi.org/10.1128/AEM.68.6.3147-3151.2002
  11. Kramer JM, Gillbert RJ. Bacillus cereus and other Bacillus species. pp. 21-70. In: Food-borne Bacterial Pathogens. Doyle MP (ed). Marcel Dekker, New York, NY, USA (1989)
  12. Chun SB, Bacillus cereus. p. 326. In: Food-born Pathogens. Jinsung Unitec, Seoul, Korea (2007)
  13. Kang CS. Study on the resistance and prevention of antibiotics. Patent 21 54: 37-43 (2004)
  14. Sveum WH, Moberg LJ, Rude RA, Frank JF. Microbiological Monitoring of the Food Processing Environment. 3rd ed. American Public Health Association, Washington DC, USA. pp. 51-74 (1992)
  15. Anonymous. Guidelines for Effectiveness Testing of Surgical Hand Scrub (Glove Juice Test). Fed. Regist. 43: 1242-1243 (1978)
  16. KFDA. Korean Food code. Korea Food & Drug Administration, Cheongwon, Korea. pp. 75-105 (2002)
  17. Yang IC, Shih DY, Huang TP, Huang YP, Wang JY, Pan T. Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. J. Food Protect. 68: 2123-2130 (2005)
  18. NCCLS. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard-9th ed. Clinical and Laboratory Standards Institute, Wayne, PA, USA. pp. 6-80 (2006)
  19. Kim SK, Kim KP, Jang SS, Shin EM, Kim MJ, Oh SS, Ryu SR. Prevalence and toxigenic profiles of Bacillus cereus isolated from dried red peppers, rice, and sunsik in Korea. J. Food Protect. 72: 578-582 (2009)
  20. Wijnands LM, Dufrenne JB, Rombouts FM, Veld PH, Leusden FM. Prevalence of potentially pathogenic Bacillus cereus in food commodities in the Netherlands. J. Food Protect. 69: 2587-2594 (2006)
  21. Park YB, Kim JB, Shin SW, Kim JC, Cho SH, Lee BK, Ahn JH, Kim JM, Oh DH. Prevalence, genetic diversity, and antibiotic susceptibility of Bacillus cereus strains isolated from rice and cereals collected in Korea. J. Food Protect. 72: 612-617 (2009)
  22. Kim SS, Park YH, Lee JS, Yoon JH, Shin YK, Rhee IK, Kim YJ. Taxonomic studies of the beta hemolysis-causing pathogen Bacillus cereus isolate from sea water. J. Microbiol. Biotechnol. 8: 67-73 (1998)
  23. Weber DJ, Saviteer SM, Rutala WA, Thomann CA. In vitro susceptibility of Bacillus spp. to selected antimicrobial agents. Antimicrob. Agents Chemother. 32: 642-645 (1988) https://doi.org/10.1128/AAC.32.5.642
  24. Chen Y, Tenover F, Koehler TM. $\beta$-Lactamse gene expression in penicillin-resistant Bacillus anthracis strain. Antimicrob. Agents Ch. 48: 4873-4877 (2004) https://doi.org/10.1128/AAC.48.12.4873-4877.2004

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