• Title/Summary/Keyword: perilla frutescens

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Changes of Physical Characteristics of Chubu Perilla Leaves(Penilla Frutescens var. Japonica HARA)during Different Storage Conditions (저장조건에 따른 추부 깻잎의 물리적 특성 분석)

  • Hur, Sang-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.410-417
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    • 2017
  • The physical properties of perilla leaves cultivated in Geumsan province were analyzed according storage conditions. The a/b values of perilla leaves increased with increasing storage period. Electronic nose composed of 12 different metal oxide sensors was used to differentiate flavors of perilla leaves. Sensitivities(delta $R_{gas}/R_{air}$) of sensors from electronic nose were obtained by principal compound analysis(PCA). Proportion of the first principal component was 93.07% at $25^{\circ}C$ and 97.81% at $4^{\circ}C$, respectively. In our result, flavor patterns of perilla leaves can be differentiated according to the storage temperature.

Transformation of Cell Wall-weakened Perilla Seedlings Using Phenolic Compound-treated Agrobacterium Cells and Recombinant Protein Expression (페놀화합물 처리 Agrobacterium 및 세포벽 약화 들깨새싹을 이용한 형질전환과 재조합 단백질 발현)

  • Chung, Il-Kyung;Shin, Dong-Il;Park, Hee-Sung
    • KSBB Journal
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    • v.24 no.6
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    • pp.598-601
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    • 2009
  • Perilla [Perilla frutescens (L.) Britt] seedlings are easy to grow and eaten as the health vegetable sprout. Two day old perilla seedlings since germination were given a mild wounding using cell wall lytic NaOH/SDS solution for infiltration with recombinant Agrobacterium cells treated with phenolic compounds. In the analysis of fluorometric GUS gene expression for the transformed perilla seedlings, GUS enzyme activity was the highest by the combined treatments of 50 mM acetosyringone and 0.5% NaOH solution containing 0.01% SDS implying a synergic effect. This result could be successfully applied for demonstrating hepatitis B virus antigen (HBsAg) protein expression.

Changes' of lipids during maturation of perilla seed(Perilla frutescens) (들깨종자의 성숙과정중 지방질의 변화)

  • Min, Young-Kyoo;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.35 no.3
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    • pp.139-145
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    • 1992
  • To investigate changes in fatty acid and lipid composition of maturing perilla(Perilla frutescens var. japonica Hara) seeds, Suweon 10 and Jeju varieties were subjected to lipid analysis. The results were summarized as follows; As perilla seeds matured, content of ether-extractable lipids increased continuously to 46.3% in Suweon and 24.0% in Jeju. In ether-extractable lipids of mature perilla seeds, palmitic acid was $9.4{\sim}18.2%$, stearic acid $2.2{\sim}5.1%$, oleic acid $19.1{\sim}32.7%$, linoleic acid $12.4{\sim}15.3%$ and linolenic acid $30.6{\sim}54.1%$ of total fatty acid. Therefore, major fatty acid were unsaturated In mature seeds while those were saturated in the early stage of seed development. As perilla seeds matured, content of neutal lipids in total lipids increased. Neutral lipids of mature seed was 70.1% in Suweon 10 and 51.5% in Jeju, respectively. Triglycerides, free fatty acid and diglycerides were major components of ether-extractable neutral lipids. Triglyceride, which showed relatively rapid increase in the early stage of development, increased to 61.4% in Suweon 10 and 68.2% in Jeju, respectively.

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Changes' of glycolipids and phospholipids during maturation of perilla seed(Perilla frutescens) (들깨종자의 성숙과정중 당지방질과 인지방질의 변화)

  • Min, Young-Kyoo;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.35 no.3
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    • pp.146-151
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    • 1992
  • To investigate changes in fatty acid and lipid composition of maturing perilla (Perilla frutescens var. japonica Hara) seeds, Suweon 10 and Jeju varieties were subjected to lipid analysis. The results were summarized as follows; As perilla seeds matured, content of glycolipid and phospholipid decreased. Glycolipid and phospholipid of mature seed were 25.4% and 4.5% of total lipids in Suweon 10 and those from Jeju were 44.5%, 4.0%, respectively. Cerebroside, galatosyl diglyceride and monogalatosyl diglyceride were major constitutents of ether-extractable glycolipids in developing perilla seeds. Monogalatosyl diglyceride, the richest constitutent in the early stage of seed development, decreased rapidly as seeds matured. In ether-extractable glycolipid of mature Suweon 10 seeds, content of linolenic acid was 51.1% which was higher than 19.4% of oleic acid of total acids. However, in Jeju variety, content of oleic acid was 31% which was higher than 18.6% of linolenic acid. Phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol and lysophosphatidyl inositol were major constituents of phospholipids. Content of phosphatidyl glycerol in developing seeds showed irregular changes in Jeju but in Suweon 10, it decreased after rapid increase in the early stage of seed development. Olieic acid ranges $28.7{\sim}35.2%$, linolenic acid $8.2{\sim}11.2%$, linoleic acid $16.4{\sim}32.5%$ and Palmitic acid $22.7{\sim}29.9%$ of total fatty acids in methanol-ertractible phospholipid of mature perilla seeds.

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Estimation of Oil Yield of Perilla by Seed Characteristics and Crude Fat Content

  • Oh, Eunyoung;Lee, Myoung Hee;Kim, Jung In;Kim, Sungup;Pae, Suk-Bok;Ha, Tae Joung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.158-163
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    • 2018
  • Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.