• Applied Biological Chemistry
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• v.42 no.2
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• pp.99-104
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• 1999

Soil actinomycetes of 703 strains were isolated from 25 sampling points in Cheju Island using 4 different media. Arginine glycerol salts agar containing soil extract was found to be the best medium for the isolation of soil actinomycetes. Soil samples from pasture land showed higher population and diversity of the actinomycetes than those from citrus field, forest, island, hill or valley. When the antibacterial activity of the 526 isolates was tested against three bacterial strains, Escherichia coli, Staphylococcus aureus and Pseudomonas solanacearum the frequency of the isolates with antibacterial activity varied much depending upon the media used for isolation and cultivation. BL106Ba, one of the 10 isolates that showed antibacterial activity against all the above 3 test strains, was chosen based upon the pH and heat stability of its antibacterial metabolites, and was identified as Streptomyces sp. based upon its cultural, morphological and physiological characteristics. The partially purified white crystalline substance obtained from the culture supematant of BL1063a through cation exchange chromatography(AG MP-50) and three times consecutive gel filtration(Sephadex G-10) showed high antimicrobial activity against gram positive and negative bacteria, but low activity against yeasts. The partially purified substance was found to contain at least four different compounds with antibacterial activity by both thin layer chromatography and high performance liquid chromatography.

• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.133-140
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• 2016

This study was evaluated individual growth and hematological changes of young starry flounder, using hyperpigmentation marks on the blind side of body after feeding extruded pellet (EP) and moisture pellet (MP) for 45 days. As a result, the fish grew up to $220.1{\pm}47.8g$ in the EP treatment and $224.7{\pm}42.4g$ in the MP treatment, so the weight gain was significantly higher in the MP treatment ($74.6{\pm}17.3%$) than in the EP treatment ($63.7{\pm}16.9%$) (P<0.05). Osmolalities of flounder after 45 days culture were similar between the treatments (P>0.05), showing values of $359.8{\pm}4.2mOsm\;kg^{-1}$ in the EP treatment and $358.5{\pm}6.2mOsm\;kg^{-1}$ in the MP treatment. Similarly, other blood chemistry indicators such as sodium, chloride, total protein, albumin, glucose, total cholesterol, BUN, creatinine, ammonia, triglyceride, cortisol, GOT, GPT and hematocrit showed no differences between the treatments. However, the free fatty acid concentration was significantly higher in the MP treatment than in the EP treatment (P<0.05).

• Journal of the Korea Society of Computer and Information
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• v.25 no.8
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• pp.99-109
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• 2020

What sports have in common is that they must use their bodies to play and lead to victory. The use of the body differs in many ways depending on the event, which part should be used as the focus. The commonality of all sports is that sports can achieve the best results by using and using parts of the body in harmony. The use of strength or muscles varies depending on the type of sport or the type of sport, so the use of the body or the results of athletic events also varies. Sports are premised on the organic use of the body, but the implications of sports can be found in that the degree of organic use does not apply equally to all sports. Sports demand organic balance and flexibility. Depending on the type of sport, you can explore which organic harmony produces more effective effects and find out which events are more beneficial to the human body. This study aimed to find out whether the organic harmony of the body actively contributes to the development of sports and a healthy mental culture. In this study, we proposed the hypothesis that the modern Pentathlon will induce the most desirable organic harmony as a global Olympic sport, and tried to verify that the hypothesis is valid and verifiable. In order to do this study, I tried to find out the organic viewpoints centered on the Miletos school, re-examine the meaning of the modern Pentathlon of games as an Olympic event, and conduct organic harmony and exploration of the modern Pentathlon of games in terms of sports.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.2
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• pp.163-170
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• 2018

The purpose of this study is to describe quantitatively the characteristics and the structure of the Act on the development, management, etc. of Marinas (the Marinas Act) by analysing its provisions using semantic network analysis. The method of semantic network analysis has its advantages in overcoming limitations of the traditional content analysis method, as it is easy for the user to understand the structure and the shape of a network by figuring out the structural network among words. The object of the analysis is the full text of Marinas Act recently revised from Chapters 1 to 4, while partial analysis is carried out respectively for each chapter from Chapters 2 to 4. The structural characteristic of the Marinas Act shows that the act focuses on the development of marinas, as its main goal is interpreted to set up hardwares and to construct facilities rather than to promote the marina industry itself. Even though some clauses for human capital development and business development are included, they are of less importance compared to the development of marina facilities. This study provides some basic information on the structural characteristics of the current act, which can be referred to in subsequent studies. In the future, it also needs to be complemented through comparative analysis with government policy outcomes and performance of diverse analytical approaches.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.96-103
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• 2009

For the control of pathogenic microorganisms, Bacillus spp. were isolated from diseased pepper fruits in Korea. Among them, Bacillus sp. IUB158-03 showed high inhibitory effect on mycelial growth and spore germination of C. gloeosporioides and Botrytis cinerea. The strain was identified as B. amyloliquefaciens IUB158-03 based on its physiological, biochemical characteristics and Microlog analysis. The highest level of antifungal substances by B. amyloliquefaciens IUB158-03 were obtained when the bacterium was cultured in medium containing 2% soluble starch, 3% yeast extract, 0.5% tryptone, 0.5% $NH_4H_2PO_4$, and 1% NaCl (pH 6.0) at $25^{\circ}C$ for 72 hrs. The antifungal substances were purified by butanol extraction, silica gel column chromatography, preparative thin layer chromatography, and high performance liquid chromatography. The purified antifungal substance was confirmed $R_f$ 0.27 by TLC. This substance exhibited antifungal activity against Fusarium solani, Rhizoctonia solani, Botrytis cineria, Alternata alternaria of plant pathogenic fungi and Trichophyton mentagrophytes, Epidermophyton floccosum, Cryptococcus neoformans of human pathogenic fungi.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.572-577
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• 2008

In order to apply strategic advanced project management to the Korean construction industry, the study conducted a comparative analysis of the project management body of knowledge of the advanced countries such as the USA and Japan as well as Europe. As a result, it was found that the connection to 'execution' of 'strategy' was highlighted as a common main issue in the theoretical background of project management body of knowledge, and strategic planning, which aligns 'program management' based on the integration of multiple project management and operations in single project with 'portfolio management' for selection and concentration, has been emphasized. Accordingly, the study built up the framework for the application of strategic advanced project management for strategic action in the Korean construction industry and proposed the following applicable solutions for implementation into the current Korean construction industry: 1) Innovate global competitiveness by preparing an 'ideology and philosophy' for the strategic action of an organization, 2) Establish a strategic 'environment' in consideration of the culture and structure of an organization, 3) Propose a strategic 'vision' for attaining a realizable goal within an organization, 4) Create an 'investment' on the portfolio in line with the strategy, 5) 'Execute' the program through the portfolio and project management. Lastly, the study brought up strategies for sustainable growth by applying strategic advanced project management to the Korean construction industry with a view to acquiring 'Performance' through the implementation and operation of their integrated system.

• Journal of Korean Home Economics Education Association
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• v.31 no.4
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• pp.19-39
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• 2019

The purpose of this study is to explore the main characteristics regarding of implicating competence-based curriculum in Finland's home economics curriculum by analyzing curriculum documents and related literature. The research findings are categorized into four main characteristics. First, home economics in Finland belongs to the 7-9 grades. The key content areas are composed of 'food knowledge & skills and food culture', 'housing & living together' and 'consumer & financial skills' at an integrated approach. Secondly, the subject competences of home economics are not presented; however, the general objectives of transversal competences are defined in Finland's curriculum document. Transversal competences describe the aspects of the objectives that are emphasized in grades 7-9 and strengthens the connectivity with each subject. Thirdly, the objectives of home economics included in the instructions in Finland consist of a content system that links learning skills, content areas, and transversal competences. Both learning skills as a role of subject competences and content areas as objectives support teachers who restructure an curriculum. Fourth, in terms of achieving subject objectives, the assessment criteria in Finland home economics is to evaluate the achievement of good knowledge and skills through actual performance. Based on the research findings, the main features of the revised curriculum in Finland include encouragement of flexibility in education systems and learner's uniqueness in schools. If the implementation of subject competences in home economics is to be strengthened, it is necessary to intergrate the knowledge and competence, require a curriculum system for implements' subject competence, carryout assessment as learning to learn, and facilitate school community and teacher community for deeper co-operation.

• Journal of Aquaculture
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• v.18 no.2
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• pp.98-106
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• 2005

A feeding trial of two extruded pellets (EPI, 45% crude protein with 7% crude lipid and EP2, 47% crude protein with 10% crude lipid)${\times}$ two satiation rates (satiation and 80% satiation)${\times}$ three feeding frequencies (three, two and one meal a day) factorial design with three replications was carried out to investigate the optimum feeding frequency and satiation rate for growth of juvenile flounder. Flounder averaging 55 g were fed a extruded pellets for 58 days. Daily feed intake was affected by dietary composition, feeding frequency and satiation rate (P<0.05). Weight gain of fish fed the EP1 and EP2 was not affected by both dietary composition and feeding frequency (P>0.05), but affected by feeding satiation rate (P<0.05). Weight gain of fish fed the each of EP1 and EP2 twice daily with satiation was the highest among treatments, but was not significantly different to that of fish fed the diets three times daily with satiation feeding. Feed efficiency and protein efficiency ratio of the fish were not affected by dietary composition, feeding frequency and satiation rate (P>0.05). Moisture, crude protein and crude lipid contents of the muscle of fish were affected by satiation rate. Moisture and crude protein contents of the liver were affected by dietary composition. Based on fish performance results, it can be concluded that the proper feeding frequency for the growth of juvenile flounder grown from 55 g to 90 g is two meals a day with satiation feeding, regardless of dietary composition used in this study.

• Culinary science and hospitality research
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• v.19 no.5
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• pp.206-224
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• 2013

The purpose of this study was to examine the formation of customer's repeated use that is real long-term commitment in franchise/chain coffee shops. 457 effective samples were analyzed using PASW 18.0 and AMOS 18.0. The results of the analysis of the relationships between user experience, customer satisfaction, customer commitment and repeated use are as follows. First, it was presented that atmosphere, price fairness and coffee product quality had a positive impact on customer satisfaction. Second, customer satisfaction had a positive impact on customer commitment and repeated use. Finally, there was the mediating effect of continuous commitment between customer satisfaction and repeated use. Based on the results of the above analysis, the managerial implications for increasing repeated use of coffee shops are as follows. First, the strategy for enhancing performance of user experience is to try to improve atmosphere(facilities, ambient/lighting), price fairness, coffee product quality(taste, flavor, temperature). Second, the strategy for forming repeated use is to enhance customer commitment(affective commitment, continuous/normative commitment).

• Journal of Life Science
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• v.20 no.5
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• pp.683-690
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• 2010

The yeast strains of Saccharomyces diastaticus produce one of three isozymes of an extracellular glucoamylase I, II or III, a type of exo-enzyme which can hydrolyse starch to generate glucose molecules from non-reducing ends. These enzymes are encoded by the STA1, STA2 and STA3 genes. Another gene, sporulation-specific glucoamylase (SGA), also exists in the genus Saccharomyces which is very homologous to the STA genes. The SGA has been known to be produced in the cytosol during sporulation. However, we hypothesized that the SGA is capable of being secreted to the extracellular region because of about 20 hydrophobic amino acid residues at the N-terminus which can function as a signal peptide. We expressed the cloned SGA gene in S. diastaticus YIY345. In order to compare the biochemical properties of the extracellular glucoamylase and the SGA, the SGA was purified from the culture supernatant through ammonium sulfate precipitation, DEAE-Sephadex A-50, CM-Sephadex C-50 and Sephadex G-200 chromatography. The molecular weight of the intact SGA was estimated to be about 130 kDa by gel filtration chromatography with high performance liquid chromatography (HPLC) column. Sodium dedecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed it was composed of two heterogeneous subunits, 63 kDa and 68 kDa. The deglycosylation of the SGA generated a new 59 kDa band on the SDS-PAGE analysis, indicating that two subunits are glycosylated but the extent of glycosylation is different between them. The optimum pH and temperature of the SGA were 5.5 and $45^{\circ}C$, respectively, whereas those for the extracellular glucoamylase were 5.0 and $50^{\circ}C$. The SGA were more sensitive to heat and SDS than the extracellular glucoamylase.