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Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken

  • Oh, J.D.;Kong, H.S.;Lee, J.H.;Choi, I.S.;Lee, S.J.;Lee, S.G.;Sang, B.D.;Choi, C.H.;Cho, B.W.;Jeon, G.J.;Lee, H.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.8
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    • pp.1065-1070
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    • 2006
  • The avian uncoupling protein (avUCP) is a member of the mitochondrial transporter superfamily that uncouples proton entry in the mitochondrial matrix from ATP synthesis. The sequencing analysis method was used to identify nucleotide polymorphisms within the avUCP gene in Korean native chicken (KNC). This study identified ten single nucleotide polymorphisms (SNPs) in the avUCP gene. We analyzed the SNPs of the avUCP gene to investigate whether polymorphism in the gene might be responsible for quantitative variations in economic traits in KNC. Three significant polymorphic sites for economic traits were avUCP C+282T (mean body weight, p<0.05), avUCP C+433T (daily percent lay, p<0.05), and avUCP T+1316C (daily percent lay, p<0.05). The frequency of each SNP was 0.125 (C+282T in avUCP gene exon 1 region), 0.150 (C+433T in avUCP gene intron 1 region), and 0.15 (T+1316C in avUCP gene exon 3 region), respectively. Among the identified SNPs, one pair of SNPs (genotype CC, C+282T and TT, avUCP C+433T) showed the highest daily percent lay (p<0.05) and mean body weight (p<0.05) and the frequency was 0.067. This study of the avUCP gene could be useful for genetic studies of this gene and selection on economic traits for KNC.

Effects of the Self-managed Seogeum Therapy among College Students with Allergic Rhinitis (서금요법 자가관리 프로그램이 대학생의 알레르기비염에 미치는 효과)

  • Park, Hong Ju;Kim, Nam Hee
    • Journal of East-West Nursing Research
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    • v.25 no.2
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    • pp.103-116
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    • 2019
  • Purpose: This study investigated the effects of the Seogeum therapy among college students with allergic rhinitis. Method: A non-equivalent control group pretest-posttest non-synchronized design was used. Intervention of Seogeum therapy has been performed from March 2 through March 31, 2017. Sixty college students diagnosed with allergic rhinitis from two different colleges in B and K cities were assigned to the experimental and control group. Data analysis were conducted using descriptive statistics, chi-square test, Fisher's exact test, and t-test. Results: There were significant differences in nose itching (t=4.89, p<.001), sneezing (t=6.26, p<.001), watery rhinorrhea (t= 4.09, p<.001), nasal congestion (t=2.36, p=.022), the percent of eosinophil (t=2.01, p=.049), perceived stress (t=2.05, p=.045), and allergic rhinitis related quality of life (t=4.33, p<.001) between the two groups. However, there were no significant differences in the percent of IgE (t=0.60, p=.553) and level of cortisol (t=0.53, p=.598). Conclusion: The results of this study indicates that the self-management of Seogeum therapy improved physical and psychological status of the college students with allergic rhinitis which ultimately improved their quality of life.

A Study of Types and Forms of Branch-road in Traditional Korean Villages (한국전통마을에 있어서 갈림길의 유형과 형태에 관한 연구)

    • Journal of the Korean Institute of Landscape Architecture
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    • v.27 no.1
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    • pp.101-109
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    • 1999
  • The purpose of this study is to analyse types and forms of branch-road in traditional korean villages. To do this, this study has selected and measured 87 branch-road cases of five traditional villages. An analysis of the case study has revealed the following results : 1. Branch-roads have four formations : 3 way, 4 way, multi-way and others. 2. Branch-roads comprise 64 percent of 3 way, 18 percent of 4 way, 8 percent of multi-way and 9 percent of others, respectively. 3. Topographic conditions have mostly affected the formation of branch-roads. 3 way of branch - roads are easily found in flat village. 4 way are multi-way of branch-road are found in semi-hilly and hilly village 4. 3 way of branch-roads have three types: T-type, Y-type, and y-type. 5. T-types are easily found in flat village, but Y-type and y-type are found in semi-hilly and hilly village. 6. Each angle of T-type is 171,99,90, respectively. It has turned out 8 degree is slightly deviated form the square. 7. Y-type of branch-road has an asymmetric form, comprising 145, 128, 87 degree of the angle, compared to 150, 150, 60 degree of symmetric one. 8. Average angles of y-type that are mostly found in hilly village are 175, 113, 72. Angle differences from each spot are 62 and 41 degree. It is assumed that 39 degree is a slope of least effort to walk. 9. 4 way of branch-roads have not only "+" type but various types of K, Y and Y, Y and y, T and y and a foot of bird. 10. It is assumed that multi-way of branch-roads, mostly found on the middle and bottom of the hilly slope, has naturally formed to connect up and down, left and right.and right.

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Germination of Carrot, Lettuce, Onion, and Welsh Onion Seeds as Affected by Priming Chemicals at Various Concentrations (당근, 상추, 양파 및 파 종자 발아촉진을 위한 Priming 약제 종류와 농도에 따른 효과)

  • Jeong, Yeon-Ok;Kang, Seong-Mo;Cho, Jeoung-Lai
    • Horticultural Science & Technology
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    • v.18 no.2
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    • pp.93-97
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    • 2000
  • This study was conducted to determine the appropriate osmotic priming chemicals and their concentrations to improve germination of carrot, lettuce, onion, and Welsh onion seeds. Each chemical, including $KNO_3$, $KH_2PO_4$, $K_3PO_4$, NaOH, $Ca(NO_3)_2$, and PEG 8000, was tested at three to four concentrations. Percent germination, number of days to attain 50% of the final germination percentage (T50), and mean number of days to germination (MDG) of primed seeds were compared with those of water-imbibed or nonprimed seeds. With the exception of 100 or 200 mM NaOH, other priming chemicals did not affect percent germination of carrot and lettuce seeds. However, the chemical priming did reduce T50 and MDG, especially with -0.5 MPa PEG 8000 for carrot and with 50 mM $K_3PO_4$ or 200 mM $KH_2PO_4$ for lettuce. Among the chemicals with no influence on percent germination did 200 mM $KH_2PO_4$ significantly reduced both T50 and MDG of onion seeds. No chemicals improved percent germination of Welsh onion seeds. Reduction in T50 and MDG was significant only with $KH_2PO_4$ and $Ca(NO_3)_2$.

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A STUDY ON THE MECHANICAL PROPERTIES OF ORTHODONTIC OPEN COIL SPRINGS (교정용 open coil spring의 역학적 특성에 관한 연구)

  • Park, Kyu-Chan;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.22 no.1
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    • pp.135-146
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    • 1992
  • It was the purpose of this study to analyze and compare the mechanical properties of ortho dontic open coil springs. Four variable factors were presented - wire diameter (.008", .009", .010"), lumen size (.030", .032", .036"), arch wire size and shape (.016" round, $ .016^{{\prime}{\prime}}{\times}.022^{{\prime}{\prime}}$ rect.) and alloy type (HiT II, Elgiloy, Sentalloy). The total 104 specimens were divided into 13 groups, and compression test was performed on an Instron test machine. The load deflection curve of each open coil spring was obtained, from which, the load-deflection relations, stiffnesses, percent recoveries were computed statistically. The results were obtained as follows: 1. When the lumen size of the coil spring remained constant, stiffness and percent recovery increased as the wire diameter increased. 2. When the wire diameter of the coil spring remained constant, stiffness and percent recovery decreased as the lumen size increased. 3. The effect of size and shape of arch wire on the coil spring was not statistically significant. 4. In alloy types, stiffness was the greatest in HiT II (55.21), Elgiloy (42.61) and Sentalloy (7.74) in that order. Sentalloy exhibited superior percent recovery and long range of action.

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A Study on the Mechanical Properties of Al-8.6% Si-3.6% Cu Alloy Cast in Plaster Mold (석고주조(石膏鑄造)한 Al-8.6% Si-3.6% Cu 합금(合金)의 기계적(機械的) 성질(性質)에 관(關)한 연구(硏究))

  • Yeo, In-Dong;Kim, Dong-Ok;Kang, In-Chan
    • Journal of Korea Foundry Society
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    • v.4 no.4
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    • pp.5-13
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    • 1984
  • This paper is presented for showing the effect of cooling rate on dendrite arm spacing, correlated with the chilling power of molding materials (conventional plaster, foamed plaster, silica sand) and section thickness, and also showing relationship between dendrite arm spacing and mechanical properties for an aluminum - 8.6 percent silicon - 3.6 percent copper alloy. Local solidification time $(t_f)$ and secondary dendrite arm spacing (d) could be varied widely in accordance with the molding materials and casting thickness, and the following relationship is obtained: $d=9.4t_f\;^{0.31}$ A good correlation between dendrite arm spacing and mechanical properties such as ultimate tensile strength, yield strength, hardness was found, that is, mechanical properties decreased in a linear manner with increase in log of secondary dendrite arm spacing. Ultimate tensile strength in conventional plaster mold casting decreased by 15 percent comparing with the sand casting, where as in foamed plaster mold casting, it decreased by 30 percent comparing with the sand casting. From those results, it has been verified that DAS might be the most representative parameter for predicting mechanical properties varing with the different cooling condition.

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A Study on the Development of Orchard Crop Security Equipment -Bark Remover of Apple Trees- (과수 관리기 개발에 관한 연구 -과수 박피기-)

  • Kim, T.H.;Jang, I.J.;Lee, J.T.
    • Journal of Biosystems Engineering
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    • v.19 no.4
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    • pp.311-317
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    • 1994
  • Warts and barks of apple trees were vector of White rot. Two devices removing warts and barks for the use of pest control on apple trees were developed and evaluated in this study, and their results are as follows ; 1. A total of 148 warts were examined to determine the average size. About 35 percent of the examined warts were 6~8mm long, 4~8mm wide and 4~6mm thick in size. About 30 percent of the examined warts were 4~6mm long, 2~4mm wide and 6~8mm thick in size. 2. Seventy-one percent of the examined barks removed were 4~8mm in thickness. 3. A blade with 30 degree of cutting angle required 3 to 22 percent less cutting energy than those with 15 and 45 degrees of cutting angles. 4. The cutting torque decreased from 31 N-cm to 12 N-cm with an increase of cutting speed from 26cm/s to 104cm/s for a feeding speed of 0.31 mm/s with the blade angle of 30 degrees. 5. The cutting torque increased from 6N-cm to 32N-cm with an increase of branch diameter from 6mm to 14mm for a feeding speed of 0.31 mm/s with the blade angle of 30 degrees. 6. Two devices mounted on a mower for removing warts and barks were evaluated and proved effective.

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Comparison of Energy Metabolisms between Laying and Non- laying Hen (산란계와 비산란계의 에너지대사 비교 시험)

  • 이영철
    • Korean Journal of Poultry Science
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    • v.13 no.1
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    • pp.31-40
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    • 1986
  • The energy metabolism with the normal laying hen and progesterone injected non-laying hen are compared. 1. The FHP of 109.7Kcal for laying hen was 25.5 percent higher than the 87.4 Kcal found for non-laying hen. 2. The MEm's of laying hen and non-laying hen were 149, and 135Kcal/Kg$\^$0.75/day and NAME's of the diets were 77 and 83 percent, respectively. For the laying hen shown negative retention in body energy during the experiment, the 77 percent NAME was the value of supporting egg production. For the non-laying hen shown the positive retention in body energy and zero egg production, the 83 percent NAME was of growth. 3. A change in body weight of 1g was comparable to 3.54 Kcal for laying hen, and 5.0 Kcal for non-laying hen, when calculated on regression equations between body weight change and body energy retention(BE). The figures indicate that the tissue energy is used with an efficiency of 70 percent for egg production. 4. Plasma level of triiodothyronine(T3) for the laying hen is appeared to be higher than that of non-laying hen, although the levels of thyroxine (T4) are equal both in laying and non-laying hen. 5. Activities of four hepatic enzymes(ATP citrate lyase, fructose diphosphate aldolase, isocitrate dehydrogenase and glutamte pyruvic transaminase) were significantly greater in the laying hen than in the non-laying hen.

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STUDIES ON THE YEASTS FOR THE BREWING OF SOY SAUCE(4) -Cultural Conditions of the Osmophilic Yeasts for Higher Concentration of NaCl- (간장발효에 관여하는 효모에 관한연구(제4보) -고농도 식염내성효모의 배양조건-)

  • Lee, Taik-Soo;Lee, Suk-Kun
    • Applied Biological Chemistry
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    • v.13 no.3
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    • pp.193-195
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    • 1970
  • The cultural conditions of the osmophilic yeasts for higher concentration of NaCl selected in the previous report were examined and the results obtained were as follows. 1) The strain $T_3\;and\;T_8$ were grown exceedingly well on the media containing 15 percent of NaCl and $T_5\;and\;T_9,\;T_{10}\;and\;T_{11}$ on the media containing 5 percent of NaCl. 2) The optimum temperature for growth of the strain $T_3\;and\;T_5$ was $30^{\circ}C,\;T_8\;T_{10}$ and $T_{11}\;was\;25^{\circ}C\;and\;T_9\;was\;35^{\circ}C.$ 3) Their lethal temperature was $60^{\circ}C$ (treatment for 10 minutes). 4) The optimum pH for growth of the strain $T_3\;and\;T_8$ was pH 4.0, $T_5$ was pH 6.0 and $T_9\;T_9\;and\;T_{11}$ was pH 5.0, respectively.

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Effect of Priming Duration and Temperature on the Germinability of Carrot, Lettuce, Onion, and Welsh Onion Seeds (Priming 처리기간 및 온도가 당근, 상추, 양파 및 파 종자의 발아력 향상에 미치는 영향)

  • Jeong, Yeon-Ok;Kim, Jong-Cheol;Cho, Jeoung-Lai
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.327-333
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    • 2000
  • This experiment was conducted to determine the effect of priming duration and temperature on germinability of vegetable seeds. Priming chemicals were -0.50 MPa PEG 8000 for carrot, 50 mM $K_3PO_4$ for lettuce, 200 mM $KH_2PO_4$ for onion, and 100 mM $Ca(NO_3)_2$ for Welsh onion seeds. Priming temperature varied from $10^{\circ}C$ to $25^{\circ}C$, and priming duration varied from 12 hours to 5 days depending on crops. Percent germination of carrot seeds was significantly affected by the seed treatment and priming temperature. Priming at $20^{\circ}C$ for 3 days was most effective in reducing number of days to attain 50% of the final germination percentage (T50) and mean number of days to germination (MDG). Radicle protrusion in the priming solution was as high as 14% when primed at $20^{\circ}C$ for 4 days. Percent germination of lettuce seeds was affected significantly by the seed treatment and priming duration. Priming lettuce seeds at $20^{\circ}C$ for 2 days increased percent germination and shortened T50 and MDG without radicle protrusion. Percent germination of onion seeds was affected significantly by the seed treatment, priming duration, and temperature. Priming at either $10^{\circ}C$ or $15^{\circ}C$ for 3, 4, or 5 days did not affect percent germination of onion seeds, but was effective in reducing T50 and MDG. Decline in percent germination of onion seeds was precipitous as the length of priming duration increased from 3 to 5 days. Percent germination of welsh onion seeds was not affected by the priming duration and temperature. Priming at $15^{\circ}C$ for 4 days was most effective in reducing T50 and MDG, increased in duration of priming at $15^{\circ}C$ or $20^{\circ}C$ increased radicle protrusion.

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