• Journal of Plant Biology
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• 제12권2호
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• pp.1-6
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• 1969

Effects of the special media on the mycelium growth in Agaricus campestris were studied. The results might be summarized as follows: 1. The mycelium growth fo Agaricus campestris were scarecely stimulated on the Peptone basal medium which was added 0.5gr. of Peptone and Dextrose basal medium which was added 1.5gr. of dextrose during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts showed a considerable growth for 144 hours. The order is as follows; Carrot-basal medium(4ml./100ml.)>Tomato-basal medium(2ml./100ml.)>Spinach-basal medium(3ml./100ml.). However, the spinach-basal medium among these three media were no significant difference.

• 대한미생물학회지
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• 제19권1호
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• pp.49-54
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• 1984

The authors isolated Vibrio parahaemolyticus from sea water, sea mud and various marine products in Busan shore area from 1981 to 1982, Among 100 isolated strains, 66 strains showed positive reaction in Kanagawa phenomenon. With the above 66 strains, the authors carred out test for detecting hemolysis activity of V.parahaemolyticus on human, rabbit, chicken, pig, goat, sheep and cow erythrocytes, in different media, such as modified Wagatsuma, nutrient, peptone and brain heart infusion agar plates media. The following results were obtained: 1. The media which can be used for Kanagawa phenomenon of V. parahoemolyticus were modified Wagatsuma, nutrient, peptone agar media, but not brain heart infusion agar medium. 2. The erythrocytes which showed positive Kanagawa phenomenon were those of human, rabbit, chicken and pig, but sheep, goat and cow erythrocytes showed no sensitivities.

• 미생물학회지
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• 제26권3호
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• pp.237-246
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• 1988

The higher bacterial numbers on clay than on sand were caused by different environmental factors. Such factors affecting the adsorption of E. coli ATCC 11775 in the sediment as follows; optimal pH range for the adsorption of E. coli ATCC 11775 was pH 7.5-pH 9.5. E. coli ATCC 11775 were shown maxima in the salinity of 18.$75%_{o}$ on sand type sediment and $12.5%_{o}$ on clay type sediment. Bacteria attached better to clay typed sediment than to sand typed sediment when organic substance was eliminated. Beef extract of 0.5%-1% concentration was found to promote the attachment of E. coli ATCC 11775 effectively. Peptone of 0.5% was enganced the attachment on the clay, and peptone of 1.3%-5%, on the sand. E. coli ATCC 11775 was found to adsorb onto benthonite with the highest efficiency and on celite with the lowest efficiency. Efficiency of adsorption by inorganic ions was shown due to higher values of ion. Adsorption was achieved in the order of $Al^{3+}, Ca^{2+}, Na^{+}$.

• 미생물학회지
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• 제26권4호
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• pp.362-367
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• 1988

The strain YL 38-3, which was capable of producing extracellular cytosine deaminase, was isolated and taxonomically examined. The isolated strain was identified to be Bacillus polymyxa YL 38-3. The optimal conditions for the enzyme production from Bacillus polymyxa YL 38-3 were investigated. The enzyme production was reached maximum level in the medium containing 0.5% glucose, 0.2% beef extract, 0.5% NaCl and 0.1% $KH_{2}PO_{4}$ (pH 6.0). And the enzyme showed the highest activity when the strain YL 38-3 was cultivated at $35^{\circ}C$ for 24 gours under the initial pH 6.0. By the additions of peptone the extracellular enzyme production was inhibited, meanwhile the intracellular enzyme production was highly stimulated. It was, therefore, deduced that peptone was related to the secretion mechanism of the enzyme from this bacterial cell.

• 한국식품영양과학회지
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• 제22권2호
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• pp.234-239
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• 1993

알긴산 분해능이 강한 균주를 자연산 미역으로부터 분리하여 동정한 결과 Vibrio sp.로 밝혀졌고, 이 균은 탄소원으로 alginate, 질소원으로 peptone, NaCl 농도 2.5%, 28$\pm$2$^{\circ}C$에서 최대의 효소활성을 보였다. 겔 여과 및 이온크로마토그래피 방법으로 정제하여 정제도가 53.7배, 비활성이 11.84U/mg의 정제효소를 얻었다. 이 정제효소를 SDS-전기영동하여 분자량을 측정한 결과 약 27,000 정도로 추정되었다.

• 한국식품과학회지
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• 제24권3호
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• pp.221-225
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• 1992

국내에서 대량생산될 수 있는 자생가능한 자원인 배추의 비전통적 이용을 위해 배추즙액에 효모 Candida utilis를 배양하여 단세포단백을 생산하는 연구를 하였다. 배추의 당함량은 $2.1{\sim}4.0%$의 범위에 있었고 배추원액을 그대로 사용하여 C. utilis를 배양했을 때보다는 배추즙액을 적절히 희석하여 배양하였을 때 증식속도가 빨라졌고 소비된 당에 대한 건조균체생산율도 17%에서 약 50% 정도로 향상되었다. 배추즙액에 C. utilis를 배양하여 단세포단백을 생산하고자 할 때는 당의 함량이 1.0% 정도로 희석하는 것이 적절하였고 당함량을 1.0%되게 조정한 배추희석액에 영양원을 첨가하여 그 영향을 평가해 보았을 때 glucose, $KH_2PO_4$나 $(NH_4)_2SO_4$는 첨가효과가 없거나 오히려 균체생산량을 감소시킨데 비하여 yeast extract나 peptone은 건조균체 생산 및 균체의 단백질 함량을 $10{\sim}20%$씩 증가시키는 효과가 있었으나 yeast extract나 peptone의 첨가량에 비해 건조균체생산량의 증가가 적어서 배추즙희석액 자체로만도 효모배양을 위한 좋은 기질임이 확인되었다.

• 한국미생물·생명공학회지
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• 제26권3호
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• pp.226-231
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• 1998

청국장으로부터 분리한 체내혈액의 응고기작에 의해 생성된 단백질인 fibrin을 분해할 수 있는 효소를 분비하는 Bacillus subtilis KCK-7을 이용하여 fibrinolytic enzyme의 생성을 위한 최적 배지조건을 조사하였다. 탄소원으로서 soluble starch 5%와 cellobiose 0.5% 첨가시 가장 높은 효소생성을 보였으며, 질소원으로는 peptone, 생대두분이 우수하였고 특히, 생대두분 2% 첨가시에 가장 높은 효과를 보였다. 최적 배지조건은 0.5% peptone, 0.3% beef extract, 0.5% cellobiose, 5% soluble starch, 2% soybean meal, 0.02% $Na_2$HPO$_4$이었다. 본 배지를 효소생산용 배지로 사용할 때 배양 48시간에 효소생성이 가장 높은 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.533-539
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• 2020

A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of Clostridium cadaveris and Clostridium sporogenes, which utilize protein as carbon source. Experimental data on changes in peptone concentration and 16S rRNA gene copy numbers of C. cadaveris and C. sporogenes were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squares analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate (μ_max), half-saturation concentration (K_s), growth yield (Y), and decay coefficient (K_d) of C. cadaveris and C.sporogenes were 0.73 ± 0.05 and 1.35 ± 0.32 h^-1, 6.07 ± 1.52 and 5.67 ± 1.53 g/l, 2.25 ± 0.75 × 10¹⁰ and 7.92 ± 3.71 × 10⁹ copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h^-1, respectively. The theoretical specific growth rate of C. sporogenes always exceeded that of C. cadaveris at peptone concentration higher than 3.62 g/l. When the influent peptone concentration was 5.0 g/l, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 10¹⁰ copies/ml at 4 h Hydraulic retention time (HRT), which indicates a 67.1% reduction of the initial population, but the wash out occurred at HRTs of 1.9 and 3.2 h. The 16S rRNA gene copy numbers of C. sporogenes gradually decreased to steady values ranging from 1.1 × 10¹⁰ to 2.9 × 10¹⁰ copies/ml. C. sporogenes species was predicted to wash out at an HRT of 1.6 h.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1252-1260
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• 2020

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03-fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

• Mycobiology
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• 제38권4호
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• pp.274-281
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• 2010

Cordyceps cardinalis was reported in Japan and the USA in 2004, and its fruiting bodies have recently been cultured in Korea. Herbarium specimens preserved at the Cordyceps Research Institute, Mushtech, Korea were revised and identified as C. cardinalis, based on morphological characters and conidial structures. Most of the C. cardinalis specimens were collected from Mt. Halla in Jeju-do. The effects of various nutritional sources and environmental conditions such as temperature and pH on mycelial growth of C. cardinalis were studied. Oatmeal agar, Martin's peptone dextrose agar, and Schizophyllum (mushroom) genetics complete medium plus yeast extract resulted in the best mycelial growth. Among carbon sources, cereals, and nitrogen sources, maltose, oatmeal, and peptone resulted in the best mycelial growth respectively. Mineral salts helped to increase growth rate but only resulted in thin mycelial density, similar to water agar. A temperature of $25^{\circ}C$ and a pH of 7 resulted in the highest mycelial growth. Based on these results, a Cordyceps cardinalis composite medium (CCM) was formulated with 1% maltose, 2% oatmeal, 1% peptone, and 2% agar. Use of the CCM resulted in slightly better mycelial growth than that of other commonly used agar media. Only organic nitrogen sources imparted a reddish pigmentation to the agar media, but this character diminished after several subcultures. A 7 day culture duration resulted in the best mycelial growth.