Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Medium Optimization for Fibrinolytic Enzyme Production by Bacillus subtilis KCK-7 Isolated from Korean Traditional Chungkookjang.

청국장으로부터 분리한 Bacillus subtilis KCK-7에 의한 fibrin분해 효소 생산 배지 최적화

  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Heo, Seok (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Bae, Dong-Ho (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Choi, Kee-Hyun (Hyosung Industry Co. Technical Research Center)
  • 이시경 (건국대학교 농업생명과학대학 응용생물화학과) ;
  • 허석 (건국대학교 농업생명과학대학 응용생물화학과) ;
  • 배동호 (건국대학교 농업생명과학대학 응용생물화학과) ;
  • 최기현 (효성생활산업(주) 기술연구소)
  • Published : 1998.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The medium optimization was investigated to maximize the production of fibrinolytic enzyme by Bacillus subtilis KCK-7 isolated from Chungkookjang, which could hydrolyze the fibrin produced through the blood coagulation mechanism in human body. The simultaneous addition of 5% soluble starch and 0.5% cellobiose to the medium as carbon sources resulted in the highest production of the fibrinolytic enzyme. Likewise, the optimized composition of medium appeared to be 0.5% peptone, 0.3% beef extract, 0.5% cellobiose, 5% soluble starch, 2% raw soybean meal and 0.02% Na$_2$HPO$_4$. In addition, the fibrinolytic enzyme production by Bacillus subtilis KCK-7 reached to the maximum level after the cultivation for 48 hr, using the optimized medium.

청국장으로부터 분리한 체내혈액의 응고기작에 의해 생성된 단백질인 fibrin을 분해할 수 있는 효소를 분비하는 Bacillus subtilis KCK-7을 이용하여 fibrinolytic enzyme의 생성을 위한 최적 배지조건을 조사하였다. 탄소원으로서 soluble starch 5%와 cellobiose 0.5% 첨가시 가장 높은 효소생성을 보였으며, 질소원으로는 peptone, 생대두분이 우수하였고 특히, 생대두분 2% 첨가시에 가장 높은 효과를 보였다. 최적 배지조건은 0.5% peptone, 0.3% beef extract, 0.5% cellobiose, 5% soluble starch, 2% soybean meal, 0.02% $Na_2$HPO$_4$이었다. 본 배지를 효소생산용 배지로 사용할 때 배양 48시간에 효소생성이 가장 높은 것으로 나타났다.

Keywords