Mycobiology

  • 제38권4호
  • /
  • Pages.274-281
  • /
  • 2010
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Growth and Cultural Characteristics of Cordyceps cardinalis Collected from Korea

  • Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Shrestha, Bhushan (Green Energy Mission/Nepal, Anam Nagar) ;
  • Han, Sang-Kuk (Division of Forest Biodiversity, Korea National Arboretum) ;
  • Kim, Soo-Young (Donghae Agricultural Technology Center) ;
  • Sung, Jae-Mo (Cordyceps Institute of Mushtech)
  • 투고 : 2010.08.17
  • 심사 : 2010.11.01
  • 발행 : 2010.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cordyceps cardinalis was reported in Japan and the USA in 2004, and its fruiting bodies have recently been cultured in Korea. Herbarium specimens preserved at the Cordyceps Research Institute, Mushtech, Korea were revised and identified as C. cardinalis, based on morphological characters and conidial structures. Most of the C. cardinalis specimens were collected from Mt. Halla in Jeju-do. The effects of various nutritional sources and environmental conditions such as temperature and pH on mycelial growth of C. cardinalis were studied. Oatmeal agar, Martin's peptone dextrose agar, and Schizophyllum (mushroom) genetics complete medium plus yeast extract resulted in the best mycelial growth. Among carbon sources, cereals, and nitrogen sources, maltose, oatmeal, and peptone resulted in the best mycelial growth respectively. Mineral salts helped to increase growth rate but only resulted in thin mycelial density, similar to water agar. A temperature of $25^{\circ}C$ and a pH of 7 resulted in the highest mycelial growth. Based on these results, a Cordyceps cardinalis composite medium (CCM) was formulated with 1% maltose, 2% oatmeal, 1% peptone, and 2% agar. Use of the CCM resulted in slightly better mycelial growth than that of other commonly used agar media. Only organic nitrogen sources imparted a reddish pigmentation to the agar media, but this character diminished after several subcultures. A 7 day culture duration resulted in the best mycelial growth.

키워드

참고문헌

  1. de Reaumur RA. Remarques sur la plante appelle'e a la Chine Hia Tsao Tom Tschom ou plante ver. Mem Acad Roy Sci Paris 1726:302-306.
  2. Vaillant S. Botanicon Parisiense, ou denombrement des plantes qui se trouvent aux environs de Paris. Leyden and Amsterdam: Briasson; 1727.
  3. Buxbaum JC. Plantarum minus cognitarum centuria IV. Petropoli: Typographia Academiae; 1733.
  4. du Halde PJ. Description geographique, historique, chronologique, politique, et physique de l'empire de la Chine et de la Tartarie Chinoise. Paris: P. G. Le Mercier; 1736.
  5. Linnaeus C. Species plantarum. Vol. 2. Stockholm: Salvius;1753.
  6. Zhu JS, Halpern GM, Jones K. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis. Part I. J Altern Complement Med 1998;4:289-303. https://doi.org/10.1089/acm.1998.4.3-289
  7. Zhu JS, Halpern GM, Jones K. The scientific rediscovery of a precious ancient Chinese herbal regimen: Cordyceps sinensis. Part II. J Altern Complement Med 1998;4:429-57. https://doi.org/10.1089/acm.1998.4.429
  8. Weng SC, Chou CJ, Lin LC, Tsai WJ, Kuo YC. Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae. J Ethnopharmacol 2002;83:79-85. https://doi.org/10.1016/S0378-8741(02)00212-X
  9. Buenz EJ, Bauer BA, Osmundson TW, Motley TJ. The traditional Chinese medicine Cordyceps sinensis and its effects on apoptotic homeostasis. J Ethnopharmacol 2005;96:19-29. https://doi.org/10.1016/j.jep.2004.09.029
  10. Kim HG, Shrestha B, Lim SY, Yoon DH, Chang WC, Shin DJ, Han SK, Park SM, Park JH, Park HI, et al. Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-κB through Akt and p38 inhibition in RAW264.7 macrophage cells. Eur J Pharmacol 2006;545:192-9. https://doi.org/10.1016/j.ejphar.2006.06.047
  11. Li SP, Yang FQ, Tsim KW. Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. J Pharm Biomed Anal 2006;41:1571-84. https://doi.org/10.1016/j.jpba.2006.01.046
  12. Holliday J, Cleaver M. Medicinal value of the caterpillar fungi species of the genus Cordyceps (Fr.) Link (ascomycetes): a review. Int J Med Mushrooms 2008;10:219-34. https://doi.org/10.1615/IntJMedMushr.v10.i3.30
  13. Paterson RR. Cordyceps: a traditional Chinese medicine and another fungal therapeutic biofactory? Phytochemistry 2008;69:1469-95. https://doi.org/10.1016/j.phytochem.2008.01.027
  14. Das SK, Masuda M, Sakurai A, Sakakibara M. Medicinal uses of the mushroom Cordyceps militaris: current state and prospects. Fitoterapia 2010;81:961-8. https://doi.org/10.1016/j.fitote.2010.07.010
  15. Winkler D. Cordyceps sinensis: a precious parasitic fungus infecting Tibet. Field Mycol 2010;11:60-7. https://doi.org/10.1016/j.fldmyc.2010.04.009
  16. Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 2007;57:5-59. https://doi.org/10.3114/sim.2007.57.01
  17. Sung GH, Spatafora JW. Cordyceps cardinalis sp. nov., a new species of Cordyceps with an east Asian-eastern North American distribution. Mycologia 2004;96:658-66. https://doi.org/10.2307/3762183
  18. Sung JM. The insects-born fungus of Korea in color. Seoul: Kyohak Publishing Co., Ltd.; 1996.
  19. Shin JC, Shrestha B, Lee WH, Park YJ, Kim SY, Jeong GR, Kim HK, Kim TW, Sung JM. Distribution and favorable conditions for mycelial growth of Cordyceps pruinosa in Korea. Kor J Mycol 2004;32:79-88. https://doi.org/10.4489/KJM.2004.32.2.079
  20. Sasaki F, Miyamoto T, Tamai Y, Yajima T. Optimum temperature and pH for mycelial growth of Cordyceps nutans Pat. (ascomycetes). Int J Med Mushrooms 2005;7:301-4. https://doi.org/10.1615/IntJMedMushr.v7.i12.270
  21. Shrestha B, Lee WH, Han SK, Sung JM. Observations on some of the mycelial growth and pigmentation characteristics of Cordyceps militaris isolates. Mycobiology 2006;34:83-91. https://doi.org/10.4489/MYCO.2006.34.2.083
  22. Lee JO, Shrestha B, Sung GH, Han SK, Kim TW, Sung JM. Cultural characteristics and fruiting body production in Cordyceps bassiana. Mycobiology 2010;38:118-21. https://doi.org/10.4489/MYCO.2010.38.2.118
  23. Sung GH, Shrestha B, Park KB, Sung JM. Cultural characteristics of Shimizuomyces paradoxus collected from Korea. Mycobiology 2010;38:189-94. https://doi.org/10.4489/MYCO.2010.38.3.189
  24. Sung GH, Shrestha B, Sung JM. Characteristics of Metacordyceps yongmunensis, a new species from Korea. Mycobiology 2010;38:171-5. https://doi.org/10.4489/MYCO.2010.38.3.171
  25. Sung JM, Shrestha B, Han SK, Kim SY, Park YJ, Lee WH, Jeong KY, Choi SK. Cordyceps diversity in Korea. Inoculum 2005;56(4):3-4.
  26. Shrestha B, Han SK, Lee JO, Lee WH, Kim BS, Park SS, Sung JM. Growth characteristics and conidial structures of different Cordyceps species. KSM Newsl 2007;19(1):87.
  27. Liu ZY, Yao YJ, Liang ZQ, Liu AY, Pegler DN, Chase MW. Molecular evidence for the anamorph-teleomorph connection in Cordyceps sinensis. Mycol Res 2001;105:827-32. https://doi.org/10.1017/S095375620100377X
  28. Shrestha B, Han SK, Yoon KS, Sung JM. Morphological characteristics of conidiogenesis in Cordyceps militaris. Mycobiology 2005;33:69-76. https://doi.org/10.4489/MYCO.2005.33.2.069
  29. Kim SY, Shrestha B, Sung GH, Han SK, Sung JM. Optimum conditions for artificial fruiting body formation of Cordyceps cardinalis. Mycobiology 2010;38:133-6. https://doi.org/10.4489/MYCO.2010.38.2.133
  30. Kornerup A, Wanscher JH. Methuen handbook of colour. 3rd ed. London: Eyre Methuen; 1978.

피인용 문헌

  1. Collected in Korea vol.39, pp.2, 2011, https://doi.org/10.4489/MYCO.2011.39.2.085
  2. In Vitro Antimicrobial and Antioxidant Activities of Ethanolic Extract of Lyophilized Mycelium of Pleurotus ostreatus PQMZ91109 vol.17, pp.12, 2012, https://doi.org/10.3390/molecules17043653
  3. Mycelial Growth of <i>Paecilomyces hepiali</i> in Various Agar Media and Yield of Fruit Bodies in Rice Based Media vol.03, pp.07, 2013, https://doi.org/10.4236/aim.2013.37071
  4. Vegetative Development of <i>Sparassis crispa</i> in Various Growth Conditions and Effect of Electric Pulse Simulation on Its Fruit Body Production vol.04, pp.05, 2014, https://doi.org/10.4236/aim.2014.45033