• Title/Summary/Keyword: pepper residues

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Determination of Quintozene Residues in Agricultural Commodities Using GC-ECD/MS (GC-ECD/MS를 이용한 농산물 중 Quintozene의 잔류분석)

  • Ahn, Kyung-Geun;Kim, Gyeong-Ha;Kim, Gi-Ppeum;Hwang, Young-Sun;Hong, Seung-Beom;Lee, Young Deuk;Choung, Myoung-Gun
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.195-203
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    • 2015
  • Quintozene, pentachloronitrobenzene (PCNB) is a contact fungicide for control of soilborne phytopathogenic fungi during cultivation of diverse crops. It was introduced to agricultural use around 1930's as a substitute for mercurial disinfectants. Although quintozene had been first registered in Korea on 1969. However, now it was banned to use due to its high residue levels in selected harvest products. Also, high possibility is expected that the residue may be contained in imported agricultural commodities as it is still used widely over the world. Therefore, this study was conducted to establish a determination method for quintozene residue in crops using GC/ECD/MS. Quintozene residue was extracted with acetonitrile from representative samples of five raw products which comprised hulled rice, soybean, Kimchi cabbage, green pepper, and apple. The extract was diluted with saline water, and n-hexane partition was followed to recover quintozene from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The quintozene was quantitated by GLC with ECD, using a DB-1 capillary column. The crops were fortified with quintozene at 3 levels per crop. Mean recoveries ranged from 79.9% to 102.7% in five representative agricultural commodities. The coefficients of variation were less than 4.3%. Quantitative limit of quintozene was 0.004 mg/kg in representative five crop samples. A GC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of quintozene in agricultural commodities.

Exposure Assessment of Pesticide-Originated Persistent Organic Pollutants in Paddy and Upland Soils in Korea (국내 논토양 및 밭토양 중 농약유래 잔류성유기오염물질의 노출량 평가)

  • Lim, Sung-Jin;Oh, Young-Tak;Ro, Jin-Ho;Kim, Seung-Yong;Joo, Hyeong-Gon;Lee, Min-Ho;Yoon, Hyo-In;Choi, Geun-Hyoung;Ryu, Song-Hee;Park, Byung-Jun
    • Korean Journal of Environmental Agriculture
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    • v.36 no.3
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    • pp.211-216
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    • 2017
  • BACKGROUND:This study was conducted to investigate residual organochlorine pesticides (ROCPs) in agricultural soils and crops. Agricultural soil samples and crop samples were collected from 93 cities and counties. METHODS AND RESULTS: Extraction and clean-up for the quantitative analysis of ROCPs were conducted by the modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. Recovery and limit of detection (LOD) of ROCPs in agriculturalsoils and crops were 76.5-103.0 and 75.2-93.2%, 0.01-0.08 and $0.10-0.15{\mu}g/kg$, respectively. Detected ROCPs in agricultural soils were ${\alpha}$-endosulfan, ${\beta}$-endosulfan, and endosulfan sulfate, the residue were 2.0-12.0, 1.2-53.1, and $2.2-329.8{\mu}g/kg$, respectively. But these pesticides in all green perilla leaf and green pepper samples were not detected. CONCLUSION: These results showed that ROCPs residues in agricultural soils were not as high as crop safety threatening.

Method Development and Validation for Analysis of Isopyrazam Residues in Agricultural Products (농산물 중 살균제 Isopyrazam의 개별 잔류분석법 확립)

  • Kim, Ji-Yoon;Kim, Ja-Young;Ham, Hun-Ju;Do, Jung-Ah;Oh, Jae-Ho;Lee, Young-Deuk;Hur, Jang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.17 no.2
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    • pp.84-93
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    • 2013
  • Validated analytical methods for isopyrazam are meager or lacking. In the present study, a single residual analytical method was developed for isopyrazam in selected commodities. Isopyrazam was analyzed in brown rice, soybean, green pepper, mandarin, cucumber, and Korean melon. We tried different solvents and methods through extraction, partition and purification steps to obtain best analytical results. For isopyrazam samples were extracted with acetonitrile, concentrated and partitioned with n-hexane, clean-up using florisil with n-hexane/ethylacetate (70/30) and analyzed with HPLC/UVD. The limit of quantitation (LOQ) for isopyrazam was 1.0 ng (S/N > 10) and method LOQ (MLOQ) was 0.04 mg $kg^{-1}$. Recovery ranged through 81.0~105.3% (syn-isomer) and 80.8~105.6% (anti-isomer) at fortification level of 0.04 (MLOQ), 0.4 (10 ${\times}$ MLOQ), and 2.0 (50 ${\times}$ MLOQ). The coefficient of variation (CV) for isopyrazam was less than 10% regardless of sample types. These results were further confirmed with LC/MS, respectively. The proposed method is highly reproducible and sensitive and is suitable for routine analysis.

Effect of Dietary Fiber on the In Vitro Digestibility of Fish Protein (식이 섬유소가 어류단백 소화율에 미치는 영향)

  • Ryu, Hong-Soo;Park, Nam-Eun;Lee, Kang-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.3
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    • pp.255-262
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    • 1992
  • In vitro digestibility of filefish, protein was substantially decreased by fiber constituents in the follow-ing order : pectin (9.97%), gum karaya (7.03%), sodium alginate (6.12%),and cellulose (1.52%). The order of reduction by fibrous residues from vegetables ranked as follows : sea tangle (12.36%), Ro-maine lettuce (11.12%), perillar leaf (8.96%), and green pepper (5.15%). The inhibitory effect of the dietary fibers towards filefish protein digestion, expressed as soybean trypsin inhibitor equivalents, in-creased with added levels, but the inhibition differed with the sources of dietary fibers. Sea tangle and sodium alginate were most active in decreasing the concentration of essential amino acid from filefish protein hydrolysis. Sodium alginate exerted an inhibitory effect on the activity of trypsin, but the other fiber constituents did not have an inhibitory potency on trypsin and bacterial pretense (Streptomyces griceus). Results supported that dietary fiber components may reduce protein digestibility through the interaction of dietary fiber components with filefish protein.

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Analytical Method for Flusulfamide as Benzenesulfonamide Fungicide, Residues in Major Agricultural Commodities (주요 농산물 중 Bezenesulfonamide계 살균제 Flusulfamide의 잔류 분석법)

  • Ahn, Kyung-Geun;Kim, Gi-Ppeum;Hwang, Young-Sun;Kang, In-Kyu;Lee, Young Deuk;Choung, Myoung-Gun
    • Korean Journal of Environmental Agriculture
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    • v.37 no.1
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    • pp.57-65
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    • 2018
  • BACKGROUND: An analytical method was developed using HPLC-UVD/MS to precisely determine the residue of flusulfamide, a benzenesulfonamide fungicide used to inhibit spore germination. METHODS AND RESULTS: Flusulfamide residue was extracted with acetone from representative samples of five raw products which comprised apple, green pepper, Kimchi cabbage, hulled rice, and soybean. The extract was diluted with large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. For the hulled rice and soybean samples, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized Florisil column chromatography. On an octadecylsilyl column in HPLC, flusulfamide was successfully separated from co-extractives of sample, and sensitively quantitated by ultraviolet absorption at 280 nm with no interference. Accuracy and precision of the proposed method was validated by the recovery experiment on every crop sample fortified with flusulfamide at 3 concentration levels per crop in each triplication. CONCLUSION: Mean recoveries ranged from 82.3 to 98.2% in five representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation (LOQ) of flusulfamide was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring technique was also provided to clearly identify the suspected residue.

Market survey on total bromide residues in foods (국내유통 식품중 total bromide의 잔류량)

  • Park, Kun-Sang;Hong, Moo-Ki;Choi, Dong-Mi;Oh, Chang-Hwan;Hwang, In-Gyun;Park, Jong-Sei
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.65-69
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    • 1998
  • To determine the amount of total bromide as 2-bromoethanol, the domestic agricultural products such as rice, barley, carrot, cucumber, apple, tomato, squash, green pepper, melon, strawberry, grape, peach, potato and celery were analyzed by GC/MSD as well as GC/ECD. The bromide was detected in most of the domestic samples and the highest bromide residue determined was 13.2 ppm in barley. The imported agricultural products including melon, kiwi, lemon, pineapple, banana, orange and grape were also analyzed for the bromide. The bromide was also detected in most of the imported ones and the highest bromide residue determined was 12.3 ppm in pineapple. In addition, the bromide residue in instant noodle spices was monitored for 4 years, recently. As results, in 1994, the bromide content was in the range of non-detection to 2.4 ppm (average 1.1 ppm) from 22 out of 24 samples; in 1995, the bromide content was 1.0 and 2.2 ppm from 2 out of 37 samples; in 1996, the bromide content was in the range of 0.7 to 37 ppm (average 12.4 ppm) from all 11 samples; and in 1997, the bromide content was in the range of 0.2 to 4.6 ppm (average 1.2 ppm) from all 59 samples. However, none of sample analyzed for the bromide was exceeded Maximum Residue Limit(s) of Korea and Codex in these survey.

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Development of Analytical Method for Fipronil Residues in Agricultural Commodities Using GC-ECD/MS (GC-ECD/MS를 이용한 농산물 중 Fipronil의 잔류 분석법 개발)

  • Ahn, Kyung-Geun;Kim, Gyeong-Ha;Kim, Gi-Ppeum;Hwang, Young-Sun;Kang, In-Kyu;Lee, Young Deuk;Choung, Myoung-Gun
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.309-317
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    • 2015
  • BACKGROUND: An analytical method was developed using GC-ECD/MS to precisely determine the residue of fipronil, a phenylpyrazole insecticide used to control a wide range of foliar and soil-borne pests.METHOD AND RESULTS: Fipronil residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, Kimchi cabbage, green pepper, and apple. The extract was diluted with saline water, and fipronil was partitioned into n-hexane/dichloromethane (20/80, v/v) to remove polar co-extractives in the aqueous phase. Florisil column chromatography was additionally employed for final purification of the extract. Fipronil was separated and quantitated by GC-ECD using a DB-17 capillary column. Accuracy of the proposed method was validated by the recovery from crop samples fortified with fipronil at 3 levels per crop in each triplication.CONCLUSION: Mean recoveries ranged from 86.6% to 106.0% in five representative agricultural commodities. The coefficients of variation were less than 10%. Limit of quantitation of fipronil was 0.004 mg/kg as verified by the recovery experiment. A confirmatory technique using GC/MS with selected-ion monitoring was also provided to clearly identify the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fipronil in agricultural commodities.

Monitoring and Risk Assessment of Pesticide Residues in School Foodservice Agricultural Products in Gwangju Metropolitan Area (광주광역시 학교급식 농산물의 잔류농약 모니터링 및 위해평가)

  • Kim, Jinhee;Lee, Davin;Lee, Mingyou;Ryu, Keunyoung;Kim, Taesun;Gang, Gyungri;Seo, Kyewon;Kim, Jung-Beom
    • Journal of Food Hygiene and Safety
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    • v.34 no.3
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    • pp.283-289
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    • 2019
  • This study was performed to monitor the residual pesticides in agricultural products used in school foodservice in the Gwangju metropolitan area. Risk assessment was also carried out based on the amount of agricultural products consumed. A total of 320 agricultural products supplied to schools in Gwangju were analyzed from 2015 to 2017. The pre-treatment and residual pesticide analysis of these products was conducted in accordance with the second method for multi-residue analysis of pesticides in the Korean food code. The hazard index was calculated by dividing the estimated daily intake (EDI) of pesticides by the acceptable daily intake (ADI). The linearity correlation coefficient for the calibration curve was 0.9923 to 1.0000, LOD 0.004 to 0.019 mg/kg, LOQ 0.012 to 0.057 mg/kg, and recovery was 79.1 to 100.2%. Residual pesticides were detected in 18 (5.6%) of 320 agricultural products used for school foodservice, and one sample of sweet potato stem (0.3%) exceeded the maximum residual limit (MRL). The detection frequency for chili peppers and bell peppers was higher than that for other agricultural products. The frequently-detected pesticides were boscalid and acetamiprid. These results showed that residual pesticide management is needed for chili pepper, bell pepper and sweet potato stem among agricultural products supplied to schools. The hazard index of bifenthrin in sweet potato stem showed the highest (64.18%), and the other pesticides were 0.03-8.23%. These results indicated that agricultural products supplied to schools in Gwangju were safe for consumption. To minimize the intake of residual pesticides, it is necessary to not only thoroughly wash agricultural products but to also ensure the expanded supply of products that are pesticide-free.

Studies on Persistence of Pesticides in Soils and Crops under Polyethylene Film Mulching Culture;III. Persistence of Soil Insecticides Endosulfan, Fonofos and Ethoprophos (폴리에틸렌 멀칭재배시(栽培時) 농약(農藥)의 토양(土壤) 및 작물체중(作物體中) 잔류(殘留)에 관한 연구(硏究);제(第)3보(報) 토양살충제 Endosulfan, Fonofos, Ethoprophos의 잔류성(殘留性))

  • Ryang, Hwan-Seung;Moon, Young-Hee;Kim, Nak-Eung
    • Korean Journal of Environmental Agriculture
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    • v.7 no.1
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    • pp.8-13
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    • 1988
  • This experiment was conducted to evaluate the effect of polyethylene film(P.E.) mulching on degradation of the soil insecticides, endosulfan(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin-3-oxide), fonofos(O-ethyl S-phenyl ethylphosphonodithioate), and ethorprophos(O-ethyl S, S-dipropyl phosphorodithioate) in red pepper, peanut, and sesame fields. Degradation of endosulfan, fonofos and ethoprophos in the soils under P.E. mulching and non-mulching followed first-order kinetics. The half-lives of fonofos, ethoprophos, ${\alpha}-$ and ${\beta}-endosulfan$ were $19{\sim}21$, $25{\sim}37$, $33{\sim}39$ and $56{\sim}81$ days, respectively. There were few differences in the half-lives of fonofos in either mulching or non-mulching concitions. However, half-lives of α-endosulfan, ethoprophos, and ${\beta}-endosulfan$ were 6, 12 and 25 days longer under P.E. mulching than under non-mulching conditions, respectively. The effect of P.E. mulching on degradation was remarkable for the slower degrading insecticides. Residues of ethoprophos in the harvested sesame under P.E. mulching and non-mulching conditions were 0.024 and 0.074ppm, respectively. Residues of fonofos in the harvested peanuts under P.E. mulching and non-mulching conditions were 0.078 and 0.017ppm, respectively. However, no endosulfan was detected in the harvested red peppers under either condition.

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Survey on the Pesticides Suspected as an Endocrine Disrupter In Agricultural Products Distributed in Seoul (서울지역 유통 농산물 중 내분비계 장애 추정농약의 잔류실태 조사)

  • Kim, Ouk-Hee;Park, Sung-Kyu;Choi, Young-Hee;Seoung, Hyun-Jung;Han, Sung-Hee;Lee, Young-Ju;Jang, Jung-Im;Kim, Yun-Hee;Jo, Han-Bin;Park, Geon-Yong;Yu, In-Sil;Han, Ki-Young
    • The Korean Journal of Pesticide Science
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    • v.15 no.1
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    • pp.36-47
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    • 2011
  • This study was performed to investigate the contaminated status of endocrine disruptor-suspected pesticides in agricultural products distributed in northern Seoul in 20l0. We analyzed 33 kinds of pesticides by multiresidue analysis method in 3,081 samples. Twelve pesticidcs were detected 600 times and violated 22 times from the agricultural products. The highest frequency of detection was procymidone and that of violation was endosulfan. The highest rates of detection divided violation times as diazinon. In agricultural products, 466 samples had pesticide residues and 22 samples violated the maximum residue limits (MRLs). Agricultural products were also classified by type and red pepper powder belonged to the others was the highest detection rate at 70.7%, fruiting vegetables 35.3%, tea leaves 23.6%, stalk and stem vegetables 21.2%, fruits 14.6% and leafy vegetables 11.2%. The rates of violation sample were stalk and stem vegetables 1.1%, leafy vegetables 1.0% and roots and tubers 0.4%. Leek was to be managed primarily because it had the high detection and violation ratio and was detected together several pesticides.