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http://dx.doi.org/10.7585/kjps.2013.17.2.84

Method Development and Validation for Analysis of Isopyrazam Residues in Agricultural Products  

Kim, Ji-Yoon (Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Kim, Ja-Young (Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Ham, Hun-Ju (Environment Friendly Agricultural Products Safety Center)
Do, Jung-Ah (Food Standards Division, Korea Food and Drug Administration)
Oh, Jae-Ho (Food Standards Division, Korea Food and Drug Administration)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Hur, Jang-Hyun (Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Publication Information
The Korean Journal of Pesticide Science / v.17, no.2, 2013 , pp. 84-93 More about this Journal
Abstract
Validated analytical methods for isopyrazam are meager or lacking. In the present study, a single residual analytical method was developed for isopyrazam in selected commodities. Isopyrazam was analyzed in brown rice, soybean, green pepper, mandarin, cucumber, and Korean melon. We tried different solvents and methods through extraction, partition and purification steps to obtain best analytical results. For isopyrazam samples were extracted with acetonitrile, concentrated and partitioned with n-hexane, clean-up using florisil with n-hexane/ethylacetate (70/30) and analyzed with HPLC/UVD. The limit of quantitation (LOQ) for isopyrazam was 1.0 ng (S/N > 10) and method LOQ (MLOQ) was 0.04 mg $kg^{-1}$. Recovery ranged through 81.0~105.3% (syn-isomer) and 80.8~105.6% (anti-isomer) at fortification level of 0.04 (MLOQ), 0.4 (10 ${\times}$ MLOQ), and 2.0 (50 ${\times}$ MLOQ). The coefficient of variation (CV) for isopyrazam was less than 10% regardless of sample types. These results were further confirmed with LC/MS, respectively. The proposed method is highly reproducible and sensitive and is suitable for routine analysis.
Keywords
Agricultural products; Isopyrazam; Limit of quantitation; Pesticide; Single residual analytical method;
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Times Cited By KSCI : 4  (Citation Analysis)
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