• Title/Summary/Keyword: pepper leaves

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Biocontrol of Red Pepper Using Mixed Culture of Antagonistic Bacterium and Phosphate Solubilizing Yeast (항진균 세균과 난용성 인산염 가용화 효모의 혼합 배양액을 이용한 고추 병해의 생물학적 방제)

  • Lee, Gun Woong;Min, Byung-Dae;Park, Sujeong;Jheong, Weonhwa;Go, Eun Byeul;Lee, Kui-Jae;Chae, Jong-Chan
    • Korean Journal of Microbiology
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    • v.49 no.4
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    • pp.398-402
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    • 2013
  • This study was to investigate beneficial effects of microbial mixture on red pepper which was capable of promoting plant growth by solubilizing insoluble phosphate as well as protecting plants from pathogenic attack. Saccharomyces sp. L13 was isolated for phosphate solubilizing activity on aluminium phosphate, tricalcium phosphate, calcium hydrophosphate, and magnesium hydrophosphate. On the other hand, Bacillus sp. L32 was isolated for antagonistic activity against Phytophthora capsisi and Colletotrichum gloeosporioides, causing Phytophthora blight and Anthracnose disease in pepper, respectively. The strain L32 exhibited antagonistic activities both under dual culture assays and detached leaves assays. The each strain under the condition of mixed cultivation exhibited the same growth rates as one under pure cultivation. In greenhouse study, the mixed culture showed the both effect of plant growth promotion and reduction of disease symptom development against P. capsisi and C. gloeosporioides providing a potential as effective microbial agent for plant husbandry.

Disease Management in Road Trees and Pepper Plants by Foliar Application of Bacillus spp. (Bacillus spp. 엽면살포에 의한 가로수 및 고추의 병 방제)

  • Chung, Joon-hui;Ryu, Choong-Min
    • Research in Plant Disease
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    • v.22 no.2
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    • pp.81-93
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    • 2016
  • Out of plant-associated bacteria, certain plant growth-promoting bacteria (PGPB) have been reported to increase plant growth and productivity and to elicit induced resistance against plant pathogens. In this study, our objective was to broaden the range of applications of leaf-colonizing PGPB for foliar parts of road tress and pepper. Total 1,056 isolates of endospore-forming bacteria from tree phylloplanes were collected and evaluated for the enzymatic activities including protease, lipase, and chitinase and antifungal capacities against two fungal pathogens, Colletotrichum graminicola and Botrytis cinerea. Fourteen isolates classified as members of the bacilli group displayed the capacity to colonize pepper leaves after spraying inoculation. Three strains, 5B6, 8D4, and 8G12, and the mixtures were employed to evaluate growth promotion, yield increase and defence responses under field condition. Additionally, foliar application of bacterial preparation was applied to the road tress in Yuseong, Daejeon, South Korea, resulted in increase of chlorophyll contents and leaf thickness, compared with non-treated control. The foliar application of microbial preparation reduced brown shot-hole disease of Prunus serrulata L. and advanced leaf abscission in Ginkgo biloba L. Collectively, our results suggest that leaf-colonizing bacteria provide potential microbial agents to increase the performance of woody plants such as tree and pepper through spray application.

Identification of Introduced Gene and Its Expression and Gene Stability Assessment for Event Selection of Genetically Modified Plant toward Approval: Cucumber Mosaic Virus Resistant Hot Pepper (상업용 유전자 변형작물 이벤트 선발을 위한 도입유전자 확인, 발현 및 세대간 안정성 평가 : 오이모자이크바이러스 저항성 GM 고추)

  • Kang, Seung-Won;Han, Bal-Kum;Lee, Tae-Ho;Kim, Eun-Ji;Lee, Gung-Pyo
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.192-200
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    • 2012
  • For the development of genetically modified plants, it is important to verify various factors which potentially affect the risk assessment as well as to establish an experimental program to produce scientific and reliable data. However, it is a time and cost consuming process to develop GM plants as well as to prepare scientific and convincible data for government's approval. Therefore, using the transgenic hot pepper tolerant to a new CMV pathotype, we attempted to suggest few methodological procedures, such as probe saturation for southern blot analysis and RT-PCR and ELISA for expression analysis, for identification and stability evaluation of inserted gene in genetically modified plant which are required for submission for approval. Ten partially overlapped probes covering full length of inserted gene were produced. We could identify that the inserted gene was stacked as a single copy as well as no partial element existed. Also, we could identify the stability of the inserted gene stacked in hot pepper using probe saturation. In the expression analysis with RT-PCR and ELISA, we also could provide the stable expression of transcript and proteins in leaves and placenta and pericarp of fruits of the CMV-resistant hot pepper.

Selection of Useful Organic Materials as an Additional Fertilizer for Organic Red-pepper Production and the Application Effect (유기농 고추 추비용 유기자원 선발 및 시용효과)

  • Choi, Du-Hoi;Sung, Jwa-Kyung;Lee, Sang-Min;Lee, Yong-Hwan;Kim, Jong-Mun;Jung, Jung-Ah;Song, Beom-Heon
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.3
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    • pp.153-157
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    • 2008
  • This study was conducted to select some effective organic materials for supplying mineral nutrients at the later red-pepper growing period under organic farming system, and to evaluate the application effect. Nutritional characteristics of organic materials, nitrogen release pattern during liquefying the selected organic materials, and crop growth were examined. Among 18 organic materials, meals such as soybean, rapeseed, cottonseed, and sunflower seed except for gluten had greater nitrogen content. Total nitrogen content liquefied solution of the mixture of organic materials and molasses or yogurt was measured after 7 days of the liquefying, thereafter, the mixture of rapeseed meal and yogurt showed the highest nitrogen, reaching about $3,000mg\;L^{-1}$. Selected liquid manure, mixture of rapeseed meal and yogurt, was applied 8 times to red-pepper plants with fertigation or foliar application. Total nitrogen of leaves at 150 days after transplanting was the highest (4.4%) in the treatment of foliar application of the selected liquid manure, whereas shoot dry weight and fresh fruit yield were the greatest in the treatment of fertigation.

Antimutagenic Effect of Green-Yellow Vegetables toward Aflatoxin $B_1$, and 4-Nitroquinoline-1-oxide (아플라톡신 $B_1$과 4-NQO에 대한 녹황색 채소류의 항돌연변이 효과)

  • 이경임;박건영;이숙희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.2
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    • pp.143-148
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    • 1992
  • The antimutagenic effects of green-yellow vegetables toward aflatoxin B$_1$(AFB$_1$) and 4-nitroquinoline-1-ox-ide (4-NQO) using the Ames assay system with Salmonella typhimurium TA98 and TA100 were studied. Forty six to fifty percent of the methanol extracts of the vegetable samples inhibited the mutagenicity induced by AFB$_1$in TA98 and TA100. Perilla leaf, lettuce, broccoli, crown daisy, water dropwort, small water dropwort, red pepper, red pepper leaves, amaranth, spinach and radish root were significantly reduced the mutagenicity of AFB$_1$(p< 0.01). Whereas 25 out of 27 samples (93%) exhibited antimutagenicity toward a direct mutagen of 4-NQO (p< 0.01. 0.05). The samples which showed the strong antimutagenicity (>60%) were cabbage, kale, lettuce, broccoli, mustard leaf, green red pepper, green sweet pepper, spinach, amaranth, soybean sprout and immature pumpkin. The juices from the several samples also showed antimu-tagenic activity toward AFB$_1$. Cabbage, perilla leaf, small water dropwort and spinach reduced TAT100 revertants dose dependently in the range of 50-500$m\ell$/plate, however, cucumber and carrot showed little effect.

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Effect of Preharvest Foliar Application of Calcium Chloride on Shelf-life of Red Sweet Pepper 'Ace' (CaCl2 엽면처리가 홍단고추의 저장성에 미치는 영향)

  • Park, Sung Min;Lee, Youn Su;Jeong, Cheon Soon
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.12-16
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    • 2001
  • This study was conducted to evaluate the effect of calcium chloride treatment (0.1%, 0.3%, and 0.5%) in red sweet pepper 'Ace' on the improvement of shelf-life and the physiological characteristics. $C_2H_4$ production and respiration rates of fruits treated with $CaCl_2$ before harvest decreased during storage of red sweet pepper at $7^{\circ}C$. Ca contents in the leaves and in the fruits showed also a greater increase in treatment of $CaCl_2$ than that in control. No difference was found in total sugar, whereas sucrose content was rapidly reduced after 20 days at $7^{\circ}C$. Ascorbic acid contents were increased by the $CaCl_2$ treatment. As Ca concentration is getting higher, the fruit decay rate was significantly reduced. From this result, we can positively conclude that foliar application with the concentration of 0.3% $CaCl_2$ and 0.5% $CaCl_2$ before harvest is effective in improving storage quality in red sweet pepper.

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Recognition of Missing and Bad Seedings via Color Image Precessing (칼라 영상처리에 의한 결주 및 불량모 인식)

  • 손재룡;강창호;한길수;정성림;권기영
    • Journal of Biosystems Engineering
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    • v.26 no.3
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    • pp.253-262
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    • 2001
  • This study was conducted to develop the vision system of a robotic transplanter for plug-seedling. A color image processing algorithm was developed to identify and locate empty cells and bad plants in the seedling tray. The image of pepper and tomato seedling tray was segmented into regions of plants, frame and soil using threshold technique which utilized Q of YIQ for finding leaves and H of HSI for finding frame of tray in the color coordinate system. The recognition system was able to successfully identify empty cells and bad seeding and locate their two-dimensional locations. The overall success rate of the recognition system was about 99%.

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Research on Development of Export Packaging for Fresh Agricultural Products (신선농산물의 수출포장개발에 관한 연구)

  • Lee, Su-Geun;Lee, Myeong-Hun
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2005.11a
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    • pp.127-139
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    • 2005
  • It is very difficult to export the fresh agricultural products to long distant countries such as America and EU without any damage. Fresh exporting products would be met very hazardous environments such as hot and cold weather, heavy trembles with rolling and pitching. Therefore, care should be taken on tight fastening the products inside the container and keeping the designated temperature and relative humidity. The physical strength of outer box should be applied differently because each agricultural product has its own character for packaging. There have been many agricultural products researches for export packaging to the U.S.A. so far. However they have never got desirable results which enough to apply it in real. The main purpose of this research is to develop optimum compressive strength of corrugated fiber board box which would be used to American export packaging of fresh agricultural products such as perilla leaves, green red pepper and spinach.

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Migration and Enrichment of Arsenic in Rock-Soil-Crop Plant System in Areas Covered with Black Shale and Slates of Okchon Zone (옥천대 흑색셰일 및 점판암 분포지역 암석-토양-농작물 시스템에서의 As및 관련 원소들의 분산과 이동)

  • 이지민;전효택
    • Economic and Environmental Geology
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    • v.36 no.4
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    • pp.295-304
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    • 2003
  • The Dukpyung and the Chubu areas were selected to investigate the migration and enrichment of arsenic and other toxic elements in soils and crop plants in areas covered with black shales. Rock and soil samples digested in 4-acid solution (HCI+HNO$_3$+HF+HC1O$_4$) were analyzed fer arsenic and other heavy metals by ICP-AES and ICP-MS, and plant samples by INAA. Mean concentration of As in Okchon black shale is higher than those of both world average values of shale and black shale. Especially high concentration of 23.2 mg/kg As is found in black shale from the Dukpyung area. Mean concentration of As is highly elevated in agricultural soils from the Duk-pyung (28.2 mg/kg) and the Chubu areas (32.6 mg/kg). Arsenic is highly elevated in rice stalks and leaves from the Dukpyung (1.14 mg/kg) and the Chubu areas (1.35 mg/kg). The biological absorption coefficient (BAC) of As in plant species decreases in the order of rice leaves>com leaves>red pepper>soybean leaves=sesame leaves>corn stalks>corn grains.