• The Korean Journal of Physiology
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• 제1권2호
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• pp.157-168
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• 1967

The entry of antipyrine and urea from the peritoneal cavity of rabbit into organ tissue and blood plasma was studied. Two hundred mg of antipyrine plus 300 mg of urea in 10 ml Ringer's solution was injected into the peritoneal cavity of anesthetized rabbit. The injection was made from above of a rabbit kept tying right side down and it enabled part of the abdominal organs (liver, intestine, kidney) was immersed in the injected solution and kept high concentration gradient throughout the experimental period. The remaining part of the organs was revered only by a thin film of the test solution. Subsequently, in this part of the organs the concentration gradient of the diffusible substances during entry was presumed to decrease as time elapsed. Four pieces of the liver tissue were taken namely, the right superficial, right deep, left superficial and left deep portions. Two were taken from the small intestine, one from the portion which was immersed in. the fluid and the other from that above the fluid mass. Both kidneys were separately analyzed. As a remote organ the gastrocnemius muscle was taken from the right leg of the animal. The intervals which were the time periods elapsed after injections were 5,7,10,15 or 30 minutes. At each point 5 animals were sacrificed and the concentrations of the test substances in the tissue water were measured. The results obtained were as follows. 1. In the liver the right portion which was immersed in the fluid showed higher concentration if the test substances than the left portion and the superficial region exceeded the deep region. The concentrations diminished as the time elapsed after infusion, particulary in the case of antipyrine, suggesting circulatory removal of the substances. In urea such decreasing tendency of the concentration was not obvious, and suggested slower removal rate of it as compared with that of antipyrine. 2. In the small intestine there was no regional difference in the concentration of the test substances. Because of the intestinal motility different portions of the intestine were seemed to have bathed in the fluid of the same concentration. In general the concentrations in the intestinal wall exceeded those of the liver, suggesting a slower removal rate than in the latter. 3. In the kidney the accumulation of the endogenous urea was predominant, and the accumulating mechanism in the renal tissue went on during the period of the experiment. Therefore it revealed increasing tendencies as the time elapsed. The penetration of the test substances in this organ from the peritoneal cavity seemed to be slower than in other abdominal organs, namely liver or small intestine. Part of the test substances in the kidney were obviously brought by the blood stream. 4. Rapid exponential decay of the concentration of antipyrine and of the osmolality of the peritoneal fluid was attributed to the extensive removal through the whole dimension of the peritoneal surface, and the remote organ such as the gastrocnemius muscle attained a fairly close value to that of the abdominal organs in less than 30 minutes. The factors which related to the absorption rate were discussed. They were the concentration gradient, permeability and the regional perfusion rate.

• 한국환경농학회지
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• 제7권1호
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• pp.21-25
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• 1988

비소(砒素)의 토양처리(土壤處理)에 의(依)한 수도근(水稻根)의 생태적(生態的) 특성(特性)을 조사(調査)하기 위해 비소를 토양이 농도별(濃度別)로 처리(處理)하고 수도(水稻)를 재배(栽培)하여 근의(根) 제형질(諸形質) 및 조직내(組織內)의 변화등(變化等)을 조사(調査)하여 얻어진 결과(結果)는 다음과 같다. 수도(水稻)의 근수(根數) 근장(根長) 및 근경(根徑)은 토양중(土壤中) 비소농도(砒素濃度)가 증가(增加)함에 따라 현저히 감소(減少)되었으며 중심주(中心柱)의 직경(直徑)도 크게 좁아졌다. 한편 토양(土壤)에 비소(砒素)를 처리(處理)한 구(區)에서는 수도근(水稻根)의 피층내(皮層內) 이상세포(異常細胞)가 발생(發生)하였으며 비소처리농도(砒素處理濃度)가 높을수록 그 수가(數) 더 증가(增加)하였다. 근의(根) 부위별(部位別) 이들(細胞)의 발생정도(發生程度)를 보면 이들 세포(細胞)는 근단부(根端部)를 제외(除外)한 중간부(中間部)와 상위부(上位部)에서만 발생(發生)하였다. 또한 이들 세포(細胞)는 표피조직(表皮組織)이 없는 근(根)의 형상(形狀)을 하고 있었다. 따라서 토양중(土壤中) 비소처리(砒素處理)에 따라 수도근(水稻根)의 피층내(皮層內) 이상세포(異常細胞)의 발생(發生)은 뿌리에 유해물질(有害物質)을 과다(過多)하게 축적(蓄積)한 근에(根)서는 뿌리의 수분(水分) 및 무기양분(無機養分)의 흡수기능(吸收機能)이 저해(沮害)를 받으므로 이를 보충(補充)하기 위한 수단(手段)으로 이들 세포(細胞)가 발생(發生)되었거나, 뿌리에 유해(有害)한 물질(物質)의 침투(侵透)에 대(對)한 방어수단(防禦手段)으로 발생(發生)된 것으로 추정(推定)된다.

• Molecules and Cells
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• 제21권1호
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• pp.42-51
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• 2006

We investigated the mechanism of contraction induced by S1P in esophageal smooth muscle cells. Western blot analysis demonstrated that $S1P_1$, $S1P_2$, $S1P_3$, and $S1P_5$ receptors existed in the cat esophagus. Only penetration of EDG-5 ($S1P_2$) antibody into permeabilized cells inhibited S1P-induced contraction. Pertussis toxin (PTX) also inhibited contraction, suggesting that it was mediated by $S1P_2$ receptors coupled to a PTXsensitive $G_i$ protein. Specific antibodies to $G_{i2}$, $G_q$ and $G_{\beta}$ inhibited contraction, implying that the S1P-induced contraction depends on PTX-insensitive $G_q$ and $G_{\beta}$ dimers as well as the PTX-sensitive $G_{i2}$. Contraction was not affected by the phospholipase $A_2$ inhibitor DEDA, or the PLD inhibitor ${\rho}$-chloromercuribenzoate, but it was abolished by the PLC inhibitor U73122. Incubation of permeabilized cells with $PLC{\beta}3$ antibody also inhibited contraction. Contraction involved the activation of a PKC pathway since it was affected by GF109203X and chelerythrine. Since $PKC{\varepsilon}$ antibody inhibited contraction, $PKC{\varepsilon}$ may be required. Preincubation of the muscle cells with the MEK inhibitor PD98059 blocked S1P-induced contraction, but the p38 MAP kinase inhibitor SB202190 did not. In addition, co-treatment of cells with GF 109203X and PD98059 did not have a synergistic effect, suggesting that these two kinases are involved in the same signaling pathway. Our data suggest that S1P-induced contraction in esophageal smooth muscle cells is mediated by $S1P_2$ receptors coupled to PTX-sensitive $G_{i2}$ proteins, and PTX-insensitive $G_q$ and $G_{\beta}$ proteins, and that the resulting activation of the $PLC{\beta}3$ and $PKC{\varepsilon}$ pathway leads to activation of a p44/p42 MAPK pathway.

• Asia pacific journal of information systems
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• 제17권2호
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• pp.123-137
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• 2007

The advent of the Internet and related Web technologies has created an easily accessible link between a firm and its customers, and has provided opportunities to a firm to use information technology to support supplementary after-sale services associated with a product or service. It has been widely recognized that supplementary services are an important source of customer value and of competitive advantage as the characteristics of the product itself. Many of these supplementary services are information-based and need not be co-located with the product, so more and more companies are delivering these services electronically. Net-based customer service, which is defined as an Internet-based computerized information system that delivers services to a customer, therefore, is the core infrastructure for supplementary service provision. The importance of net-based customer service in delivering supplementary after-sale services associated with product has been well documented. The strategic advantages of well-implemented net-based customer service are enhanced customer loyalty and higher lock-in of customers, and a resulting reduction in competition and the consequent increase in profits. However, not all customers utilize such net-based customer service. The digital divide is the phenomenon in our society that captures the observation that not all customers have equal access to computers. Socioeconomic factors such as race, gender, and education level are strongly related to Internet accessibility and ability to use. This is due to the differences in the ability to bear the cost of a computer, and the differences in self-efficacy in the use of a technology, among other reasons. This concept, applied to e-commerce, has been called the "e-commerce divide." High Internet penetration is not eradicating the digital divide and e-commerce divide as one would hope. Besides, to accommodate personalized support, a customer must often provide personal information to the firm. This personal information includes not only name and address, but also preferences information and perhaps valuation information. However, many recent studies show that consumers may not be willing to share information about themselves due to concerns about privacy online. Due to the e-commerce divide, and due to privacy and security concerns of the customer for sharing personal information with firms, limited numbers of customers adopt net-based customer service. The limited level of customer adoption of net-based customer service affects the firm profits and the customers' welfare. We use a game-theoretic model in which we model the net-based customer service system as a mechanism to enhance customers' loyalty. We model a market entry scenario where a firm (the incumbent) uses the net-based customer service system in inducing loyalty in its customer base. The firm sells one product through the traditional retailing channels and at a price set for these channels. Another firm (the entrant) enters the market, and having observed the price of the incumbent firm (and after deducing the loyalty levels in the customer base), chooses its price. The profits of the firms and the surplus of the two customers segments (the segment that utilizes net-based customer service and the segment that does not) are analyzed in the Stackelberg leader-follower model of competition between the firms. We find that an increase in adoption of net-based customer service by the customer base is not always desirable for firms. With low effectiveness in enhancing customer loyalty, firms prefer a high level of customer adoption of net-based customer service, because an increase in adoption rate decreases competition and increases profits. A firm in an industry where net-based customer service is highly effective loyalty mechanism, on the other hand, prefers a low level of adoption by customers.

• 생태와환경
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• 제37권4호통권109호
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• pp.436-447
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• 2004

습지를 규정하는 주요한 특징의 하나인 습지식물은 장기간의 침수로 인해 혐기성 상태로 존재하는 습지 퇴적물에서 생존을 위한 특별한 적응방법을 발달시켰다. 식물체내에 넓게 분포하고 있는 다공성의 세포는 공기중의 산소를 뿌리로 운반하기 위한 통로로 작용하며, 농도차이에 의한 확산과 압력차이에 의한 대류에 의하여 산소가 운반되어진다. 이러한 식물체 내에서의 산소이동은 식물이 혐기성 퇴적물 속으로 뿌리를 내리고 생존하게 하는 주요한 기작이 된다. 뿌리로 이동되어진 산소는 혐기성 퇴적물로 확산되어져서 뿌리주변의 퇴적물은 산화상태로 변화시키고, 뿌리의 호흡, 미생물의 호흡, 미생물에 의한 유기물 분해반응을 촉진시키게 된다. 또한 습지식물은 생장에 필요한 수분을 뿌리로 흡수하며, 이는 지표수와 퇴적물내 공극수가 뿌리주변으로 이동하게 되는 추진력이 된다. 습지 퇴적물은 식물의 사체에서 기인하는 유기물에 의해 수리학적 전도도가 작아서 퇴적물내 물의 움직임이 미미하나, 식물에 의한 물의 흡수는 퇴적물내 물의 움직임을 촉진시키게 된다. 이러한 식물의 특별한 적응기작은 해부학적, 형태학적, 생리학적으로 많은 연구가 수행되어져 왔으나, 이러한 적응기작들에 퇴적물내 생지화학적 반응에 미치는 영향에 대한 연구는 미비한 수준에 머물러있다. 퇴적물내 생지화학적 반응들은 수체에서 유입된 미량 오염물질의 이동 및 변형과정에 영향을 미치게 되므로 식물의 작용에 의한 생지화학적 반응의 변화들은 미량 오염물질의 거동에 영향을 미치게 되며 나아가 수자원과 수질 생태계에 영향을 초래하게 된다. 따라서 식물의 존재와 성장에 따른 퇴적물내 생지화학적 반응의 변화는 생태학적 환경에서 습지의 중요성을 인식하는데 필요한 연구과제라 사료된다. 난이도, 변별도 등에서 유사하므로 당분간 계속 사용하여도 될 것이다. 따른 변화(變化)는 볼 수 없었다. ATP 첨가(添加)로서는 0.30mM의 농도(濃度)에서 0.15 mM의 농도(濃度)에 비(比)하여 Young 율(率)이 낮았다. 3) 외경동맥(外經動脈)의 종절편(縱切片)의 Young 율(率)은 생리적식염수(生理的食鹽水)에 둔 군(群)에서는 15분(分), 45분(分) 및 75분(分)에서 각각(各各) 2.12, 2.48 및 $2.46{\times}10^7 dyne/cm^2$으로서 실험초기(實驗初期)에 비(比)하여 후기(後期)에서 Young 율(率)이 약간(若干) 높은 경향(傾向)을 나타내었고, 이러한 경향(傾向)은 ATP의 첨가(添加)로서도 비슷하였다.수량(收量)과 자실체형성(子實體形成) 소요일(所要日)의 관점(觀點)에서 보면 C/N율(率) 30.46이 어느정도 적당(適當)한 것 같다. 4. Thiamine $50{\mu}g%,\;KH_2PO_4$ 0.2%, $MgSO_4{\cdot}7H_2O$는 $0.02{\sim}0.03%$일때 균사(菌絲)와 자실체(子實體) 생육(生育)이 우수(優秀)하였으며 미량원소(微量元素)로서는 $FeSO_4{\cdot}7H_2O$,\;ZnSO_4{\cdot}7H_2O$ 및 $MnSO_4{\cdot}5H_2O$가 공존(共存)하면 생육촉진(生育促進)의 상승효과(相乘效果)가 인정되었으나 3이원소(元素)중 Mn이 결핍(缺乏)하면 균사(菌絲)와 자실체(子實體)의 생육(生育)이 다소 저하되었다. 이들 염류(鹽類)의 최적농도(最適濃度)는 각각 0.02mg%이었다. 5.

• 대한지리학회지
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• 제29권3호
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• pp.266-280
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• 1994

최근 12년간(1982-1993)의 고도별 상층일기도(850, 700, 500hPa)와 6시간 간격의 지 상일기도 그리고 지상의 기상요소를 분석하여 영서지방에서 높새바람이라고 알려져 있는 푄 현상의 특성을 밝히고자 하였다. 푄현상은 대체로 3월 21일부터 8월 10일까지 기간에 연평 균 28회 출현한다. 반순별로 10회 이상 출현하는 기간은 3월 21-25일, 4월5일-15일, 5월25일 -6월10일, 그리고 6월26-30일이다. 푄현상은 한반도가 오호츠크해기단의 영향하에 있거나, 고기압의 중심이 동해 또는 한반도 북부에 위치하고 있을 때 현저하다. 푄현상의 특성인 이 상고온 및 이상건조현상을 기준으로 푄의 강도를 평가할 때 양사면의 일최고기온의 차가 14.5$^{\circ}C$에 달하기도 하지만 대체로 5.0-7.5$^{\circ}C$(61%)이다. 전날에 비해 일최고기온이 7.6$^{\circ}C$가 높 아진 경우도 있다. 최소상대습도의 강도는 50%를 넘는 경우도 있으나 30% 이하인 사례가 2/3에 달한다. 푄현상의 강도는 6월에 가장 강하나 주민들은 밭작물의 파종과 이앙기인 봄 철에 더욱 심각하게 푄을 인식한다. 푄현상은 9일간 계속되기도 하였으나 55% 이상이 1일 안에 소멸한다. NOAA AVHRR와 GMS의 영상에서 구름의 분포를 참조하여 지상 기상요소 를 분석한 결과 푄현상에서는 풍상강수형, 풍상.정상강수헝, 풍상무강수형 등 3개 유형이 발견된다. 그런데 제 3유형은 열역학적 이론만으로는 설명하기 어려우므로 풍하파와의 관련 여부, 산지지형 또는 기단섭동과의 관련성 등을 밝힐 필요가 있는데, 이를 위하여는 고층기 상자료와 보다 조밀한 기상관측망이 요구된다.