• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.44-44
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• 2019

This study aimed to develop a suitable method for inducing the proliferation of prothallus and producing sporophytes of rock polypody (Polypodium vulgare L.). The prothalli used in all experiments were obtained from spore germination and sub-cultured for 8-week intervals. The most appropriate media for prothallus propagation were investigated by culturing 300 mg of prothallus in MS ($1/4{\times}$, $1/2{\times}$, $1{\times}$, and $2{\times}$ strength) medium and in Knop medium for 8 weeks. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\pm}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark). Fresh weight of prothalli was 4.8 g on $1{\times}$ MS, 4.5 g on $1/2{\times}$ MS and 4.3 g on 1/4 MS medium. To select a suitable soil combination for sporophyte formation, 1.0 g of prothallus was ground with distilled water, spread in five combinations onto different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and then cultivated for 13 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). The results showed that a mixture containing a 2:1 (v:v) ratio of horticultural substrate and perlite, increased sporophyte formation to 462.5 sporophytes per pot (7.5 cm2). The other soil substrates produced from 314.5 to 405.3 sporophytes per pot. Therefore, our results will provide conditions suitable for mass production of Polypodium vulgare L.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.11-19
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• 1984

Potted, germinating Pinus rigida ${\times}$ P. taeda seedlings were inoculated with Pisolithus tinctorius (Pt) ectomycorrhizal fungus to test the effectiveness of Pt in relation to organic amendment and changes in soil fertility and soil texture. Pt was cultured as mycelia in vermiculite-peat moss mixture with nutrients and added to sterilized pot soils with or without organic amendment (fully fermented compost) at three soil texture levels (sand, loamy sand, and sandy loam) in a factorial design. Plants were grown in a greenhouse for 4 months and harvested to compare their growth with non-mycorrhizal plants and plants infected by natural fungi. Regardless of sod texture, soil fertility, or organic amendment, seedlings inoculated with Pt were better in dry weight and height than non-mycorrhizal plants or those infected by natural fungi. An exception was observed in the most fertile soil (0.075% N and 1.32% organic matter content in sandy loam with organic amendment), where non-mycorrhizal plants were slightly bigger (8%) and heavier (18%) than Pt-inoculated plants. In over-all average, Pt-inoculated seedlings were 30% taller and 107% heavier than those infected by natural fungi and 31 % taller and 60% heavier than non-mycorrhizal plants. Growth stimulation of seedlings by Pt was more pronounced in less fertile sand soil when organic was not amended. Mycorrhizal frequency of Pt (% of mycorrhizal root tips) was reduced to about half (from 84 to 33% in sandy loam and from 77 to 40% in loamy sand) by organic amendment, while that of natural fungi was not significantly affected. Severe nitrogen deficiency was observed in the needles of non-mycorrhizal plants (1.38% N), while both Pt-inoculated plants (1.68% N) and those infected by natural fungi (1.89% N) did not develop symptom, suggesting an active role of mycorrhizae in absorption of soil nitrogen. Top to root ratio increased with organic amendment to non-mycorrhizal plants, but was not significantly affected by fungal treatment. It was concluded from this study that relative effectiveness of Pt was determined by soil fertility. Organic amendment to less fertile sand soil increased effectiveness of Pt, while the same amendment to more fertile loamy sand and sandy loam decreased effectiveness of Pt. Benefits of Pt mycorrhizae would be expected most either when organic was not added to the soil, or when soil nutrients were not abundant.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.358-364
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• 2006

This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as the growth index of the mycelia. Among the various solid matrixes, the granitic soil, perlite and mixture of the two supported the growth most. Barely flour appeared to be very effective on the stimulating of the mycelial growth when added to the solid matrix. An mixture of the matrix contained an even (1:1:1:1, v/v/v/v) mixture of granitic soil, perlite, vermiculate and pine sawdust. T. matsutake started growth 2 weeks after inoculation and reached stationary growth phase after 8th weeks in the solid matrix mixture. The mycelial density in the solid matrix was 7 times higher than that in fairy-ring soil. In addition, 30~70% water content and 10% humus soil in the solid matrix also supported good growth suggesting that T. matsutake needs humus soil for a nutrient sources. The solid matrix developed in the present study could be used to study physiological characteristics of T. matsutake as well.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.4
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• pp.1-9
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• 2002

This study is carried out to make the environmentally affinitive porous planting block for revegetation and to make a effective program for greening plans. The summary is shown below. 1. In order to get stronger intensity and distribute proper porosity in the block for planting, the cements mixed with fine soil were used and the finer in soil grains gives the stronger in intensity of the cements. Use of the furnace slag cements instead of the portland cements showed relatively stronger in intensity of the block. The intensity of the block became stronger when the mixed ratio of the cements to soil is 5 : 1, but the pore space ratio was lower. The percolate pH of the portland cements after one month of treatment was 13.1 but the percolate pH of the furnace slag cements was shown lower. To mold proper porous planting blocks, the proper combination of additives such as the dehydrating agent, elastic agent and adhesives into the mixture of cements and soil gives better effectives. 2. After molding the porous planting blocks, it gave a better result when the grains of the filler made of peat moss, upland soil and compound fertilizer were smaller than 2 mm in size. Shaking of the filling materials also gave the better result, but it took more time and cost much more. Therefore, it was better when the filling materials were mixed with water first then flew down for stuffing. 3. It was necessary to cover with soil after seeding or planting on the porous planting blocks. The proper thickness of the soil to help root development and keep moisture is about 3~5 cm. 4. The plants for planting on the porous planting block were required stronger in the growth condition of their roots and their environmental adaptability. The average germination percentage and rate of Platycodon grandiflorum on the porous planting block were 88.8% and 85% accordingly and their rate is very uniform. The germination rates of Dianthus superbus var. longicalycinus and Taraxacum officinale were more than 50%. These grass species, Chelidonium majus var. asiaticum, Lysimachia mauritiana and Scabiosa mansenensis were the suggested biennial grasses in the planting area where exchanging of the seedling or nursery plants was not necessary because their germination rates were 59.3, 45.6 and 40.3% accordingly. Viola kapsanensis, Chrysanthemum sp., Taraxacum sp. and Iris ensata var. spontanea are the grass species that could be used by seeding for greening. However, the germination rate of Solidago virga-aurea var. asiatica, Aster scaber and Lythrum anceps were lower than 10%. The coverage ratio of Ixeris stolonifera is more than 80% after 60 days seeding and the root length of most of species are more than 10 cm except Iris ensata var. spontanea and Platycodon grandiflorum because their root developed thicker than other species.

• Korean Journal of Environmental Agriculture
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• v.14 no.1
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• pp.82-87
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• 1995

The standardization of test procedures and reproducibility of the toxicity data are prerequisite for the toxicity testing with the earthworm culturing in the laboratory. No in-depth study on culturing conditions of earthworms has been conducted in Korea, even of massive cultural practice is common for composting and production of biochemicals. The earthworms, Lumbricus rubellus and Eisenia foetida, were cultured in three kinds of artificial soil substrates(I, II and III) based on the OECD Guideline, which consist of different ratios of components (sand, sphagnum peat and kaolinite), and fed with a mixture of grain powders. During the period of culturing, the body weight and reproduction parameters were measured. L. rubellus showed the best results for increasing body weight and cocoon production in the artificial soil substrate(I) compared with E. foetida. The cocoon production was significantly high in both species cultured in the artificial soil substrate(I) among the three kinds of soil substrates, but the cumulative cocoon production of L. rubellus was 11 cocoon per worm compared with 3.7 cocoons per worm of E.foetida. L. rubellus, therefore, was more prolific than E. foetida in these culture schemes. The cumulative mortality in both species was less than 10%, and the number of juvenile worms per cocoon ranged from 1.5 to 2.3 and thus did not show any relationships with soil substrates or species. From these data, the culture of L. rubellus in the laboratory could be standardized, but for E. foetida, further study would be necessary to establish the optimal growth conditions in the laboratory.

• The Korean Journal of Pesticide Science
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• v.4 no.4
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• pp.56-60
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• 2000

A study was performed to determine the maintenance conditions of the earthworm, Lumbricus rubellus, for the acute toxicity test. To fine out climatic and soil conditions, the earthworms were maintained in artificial soil consisting of sand, clay mineral and peat at different levels of conditions for 14 days. Lumbricus rubellus led to an increase of biomass at temperature $22{\pm}2^{\circ}C$, soil pH $7.0{\pm}1$ and moisture 40%. And four fungicides were tested for acute toxicities to Lumbricus rubellus, according to the optimum condition. The test earthworms were exposed to each pesticide with various concentration gradients. After 14 days, the number of surviving earthworms and their weight alteration during the test period was determined. The 14-day $LC_{50}$ values for the Lumbricus rubellus, of carbendazim, benomyl, thiophanate-methyl and thiabendazole were determined to be 59, 53, 64 and 36 mg/ kg soil dry weight, respectively.

• Journal of the Korean Geotechnical Society
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• v.22 no.9
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• pp.61-68
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• 2006

Recently the large scale civil engineering projects are being implemented by reclaiming the sea or utilizing seashore and river embankment areas. The reclaimed land and utilized seashore are mostly soft ground that doesn't have sufficient bearing capacity. This soft ground consists of fine-grained soil such as clayey and silty soils or large void soil like peat or loose sand. It has high ground water table and it may cause the failure and crock of building foundation by uplift pressure and ground water leakage. In this study, the permittivity and the transmissivity were evaluated with the applied normal pressure in the laboratory. The laboratory model tests were conducted by utilizing geocomposite drainage system for draining the water out to release the uplift pressure. The soil used in the laboratory drainage test was dredged soil from the reclaimed land where uplift pressure problems can arise in soil condition. Geocomposite drainage system was installed at the bottom of apparatus and dredged soil was layered with compaction. Subsequently the water pressure was supplied from the top of specimen and the quantities of drainage and the pore water pressure were measured at each step water pressure. The results of laboratory measurements were compared with theoretical values. For the evaluation of propriety of laboratory drainage test, 2-D finite elements analysis that can analyze the distribution and the transferring of pore water pressure was conducted and compared with laboratory test results.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.102-106
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• 1997

The inluence of styrofoam-panel thickness of floating frame on the seedling growth of flue-cured tobacco(Nicotiana tabacum L.) in the newly developed direct-seed float system was investigated . Floating frame used instead of styrofoam-tray consisted of styrofoam-panel (106×108cm) for floating, eight holes(1.Sx2S.0 cm) to uniform the water, and nutrition supply far seedling and water and nutrition absorption cloth placed on the styrofoam-panel. Each floating-frame may lay eight plastic-trays on it. Eight grades of styrofoam-panel thickness from 26 mm to 46mm and two kinds of media were used in the greenhouse system. Dry cells were found from the styrofoam-panel thickness of 43mm in carbonized chaff, compost and original soil rate of L3-3(v/v) media, and of 37mm in peat and perlite rate of 8-2(v/v) media. The thinner styrofoam-panel produced more tender and succulent seedling with the more trunk and the less root weight. When considering the appearance of dry cell, seedling growth, producing healthy tobacco seedlings, and utilization of styrofoam-panel for two kinds of medias the ideal styrofoam-panel thickness were suggested to be around 34mm for flue-cured tobacco reeling production in the newly developed direct-seeding float system. Key words : tobacco seedlings, float system, medium, styrofoam pannel, dry cell.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.193-197
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• 2004

In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 $\mu{M}$) or Kin (2-10 $\mu{M}$). Optimum response in terms of per cent cultures responding (89%) and the number of embryos per explant (16) were observed on MS medium supplemented with 8 $\mu$M BAP. The emergence of several somatic embryos on the adaxial side of the leaf segments was observed one month after the culture. Germinated somatic embryos were grown up to about 1.5 cm length before transferring to maturation medium. For maturation, the individual embryos were isolated and transferred to MS medium supplemented with BAP (5 $\mu{M}$) and NAA (0.5 $\mu{M}$). The plantlets emerged from the embryos were transferred to soil containing 1 peat: 1 sand with 90% success. The embryos were formed directly on the leaf segments without any callus phase. Direct regeneration of somatic embryos is important for the conservation of this endangered species, as rare somaclonal variants are likely to arise than from indirect regeneration.

• Geomechanics and Engineering
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• v.33 no.4
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• pp.401-414
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• 2023

Organic clays are ideal habitat for flora and fauna. From a geotechnical perspective, organic clays are soft, weak, variable, heterogeneous and flocculated. Portland cement is a universally common stabiliser. However, some organic acids in soil inhibit full hydration and expose cementation products to rapid dissolution. This paper investigates scopes for use of C₃S cement to enable durable cementation. Prospects of using PP fibre alongside with C₃S cement, scopes for partial replacement of C₃S cement with a plant-based nanosilica and evolution of binders are then investigated. Binding mixtures here mimic the natural functions of rhizoliths, amorphous phases, and calcites. Testing sample population include natural and fibre-reinforced clays, compact mixes of clay - C₃S cement, clay - nanobiosilica, and clay, C₃S cement and nanobiosilica. Benefits and constraints of C₃S cement and fibres for retaining the naturally flocculated structure of organic clays are discussed. Nanobiosilica provides an opportunity to cut the C₃S content, and to transition of highly compressive organic clays into an engineered, open-structured medium with >0.5 MPa compressive strength across the strains spanning from peak to 1.5-times peak.