• 제목/요약/키워드: patch test

검색결과 301건 처리시간 0.025초

In vitro Alternatives to Skin Irritation Test

  • Shin, Dae-Sup;Kim, Dai-Byung;Ryu, Seung-Rel;Lee, Sun-Hee;Koh, Jae-Sook;Park, Won-Sae;Kim, Pu-Young
    • Biomolecules & Therapeutics
    • /
    • 제3권3호
    • /
    • pp.242-244
    • /
    • 1995
  • In vitro cell culture system has been proposed as a promising alternative model to in vivo skin irritation test. These studies were performed to screen the cytotoxicity effects of surfactants using normal human skin fibroblasts. Cell membrane integrity assessed by the leakage of lactate dehydrogenase (LDH) and mitochondrial integrity by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromides reduction test were affected in a dose dependent manner. The irritation potential of surfactants to human skin patch test, and the changes of capillary permeability by rabbit intradermal safety test were assessed as in vivo methods. Our results suggest that LDH leakage assay and MTT reduction test using cultured human fibroblasts could be predictive for the irritancy of various surfactants in human, and LDH assay is superior correlated with in vivo test (r=0.886) to MTT test with in vivotest (r=0.757).

  • PDF

Comparison of Carbon Stock Between Forest Edge and Core by Using Connectivity Analysis (연결성 분석을 활용한 산림의 주연부와 내부의 탄소저장량 비교)

  • Sung, Sun-Yong;Lee, Dong-Kun;Mo, Yong-Won
    • Journal of Korean Society of Rural Planning
    • /
    • 제21권4호
    • /
    • pp.27-33
    • /
    • 2015
  • Forest ecosystem is considered as an important stepping stone to minimize the impact of climate change. However, the rapid urbanization has caused fragmentation of forest ecosystem. The fragmentation of forest patch results in edge effect which brings about adverse impacts on forest function and structure. Degradation of forest ecosystem decreases carbon sequestration because edge effect reduces productivity. Therefore, we analyzed the impact of forest edge effect on forest ecosystem carbon stock change in Seongnam-si, Gyeonggi-do. We used connectivity analysis to determine forest edge and core area. The field study sites were selected with considering forest age, density, class and soil type. Secondly, forest carbon stock was calculated with allometric equation. The soil carbon stock was derived from Walkely-Black method. Lastly, Mann-Whitney test was conducted to validate differences between carbon stock in edge and core area. As a result of study, the connectivity analysis was effective to determine forest edge and core. The core and edge of forest patch showed different composition of tree species and soil properties. Carbon stock per tree in the edge area was lower than that in the core area. However, the difference of soil organic carbon content between the edge and core were relatively small. This assessment can be applied for the conservation of forest patch as well as quantitative assessment on the forest carbon stock change caused by fragmentation.

Occurrence of Rhizoctonia Blight of Zoysiagrasses in Golf Courses in Korea (국내 골프장 한국잔디의 라이족토니아마름병 발생)

  • 심규열;김진원;김희규
    • Korean Journal Plant Pathology
    • /
    • 제10권1호
    • /
    • pp.54-60
    • /
    • 1994
  • Incidence of Rhizoctonia blight ranged from 22.2% to 100% in the golf courses at six geographical locations in Korea from 1989 to 1993. Rhizoctonia blight occurred more severly in southern area than in northern area. Fifty seven isolates of Rhizoctonia solani obtained from diseased parts of zoysiagrasses were grouped to AG2-2 by anastomosis test. Pathogenicity testes revealed that this pathogen was strongly pathogenic to Korean lawngrasses(Zoysia japonica, Z. matrella, Z. tenuifolia), but not pathogenic to creeping bentgrass(Agrostis palustris), bermuldagrass(Cynodon dactylon), Kentucky bluegrass(Poa pratensis), perennial ryegrass(Lolium prenne), and creeping red fescue(Festuca rubra subsp. rubra L.). The isolation frequency of R. solani AG2-2 fro sheaths of the infected plants was the highest by 91.67%, and that from stolons and roots was 11.13% and 5.63% respectively. The pathogen was not isolated from the leaves. Population density of R. solani in the lawn of large circular patch was highest on surface soils down to 1 cm deep with the value of 4.9$\times$104 (CFU/g soil), but below 1 cm population density decreased sharply down to 0.8~9.8$\times$103 (CFU/g soil). Horizontal distribution of propagules in turfgrass soil was higher in the margin than in center of patch, where the number of propagules was similar to these of healthy looking soils close to the margin of diseased patch. The meteorological factors influencing the outbreak of the disease were temperature, the number of rainy days and precipitation. Optimum temperature for disease development of Rhizoctonia blight in field was 20~22$^{\circ}C$, and that for hyphal growth of R. solani AG2-2 in vitro was 25~3$0^{\circ}C$. In Pusan area, Rhizoctonia blight first occurred in late April and rapidly developed in late June. The disease slightly decreased during July to August and developed again in late September in 1993. The monthly disease progress in Pusan area was similar to that in Kyeonggi province.

  • PDF

Use of Oil Red O Staining Method in Non-Comedogenic Test for Cosmetics (화장품의 면포 비유발 평가에서 오일 레드 오 염색법의 응용)

  • Lee, Sun Hwa;Lee, Jung Im;Kim, Yoo-Ri;Lee, Bum Chun;Kang, Min Ji;Choi, Kwang Seong;Moon, Tae Kee
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • 제39권3호
    • /
    • pp.215-224
    • /
    • 2013
  • It has been reported that certain ingredients added to cosmetics clog the skin pores and this can cause outbreaks of comedones which are the primary sign of acne leading to inflammatory acne. This research aims to establish objective evaluation criteria for non-comedogenic cosmetics suitable for acne prone skin. The research has been carried out to examine non-comedogenic test performed in foreign clinical institutions and to establish the evaluation method for detecting comedones outbreaks through repetitive closed back-patch test, Also, usability evaluation on face skin is performed additionally to the same subjects. The analysis of the comedones collected through repetitive closed back-patch test confirmed that the test products, moisturizer and sunscreen product, did not cause comedones. These results had no correlation with the analysis result of the comedones collected from face skin or visual evaluation of acne by Global Acne Grading System (GAGS) in face usability test. Additionally, Oil red O staining was performed on the collected comedones specimen for easy distinction of comedones from hair follicle in image analysis. The analysis result of stained specimen showed higher precision than that of non-stained specimen. This study established a new version of non-comedogenic test for cosmetics, whose objectivity and reliability were improved by inclusion of comedones staining step.

Fiber Optic Smart Monitoring of Concrete Beam Retrofitted by Carbon and Glass Sheets

  • Kim Ki-Soo
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 한국콘크리트학회 2004년도 춘계 학술발표회 제16권1호
    • /
    • pp.486-489
    • /
    • 2004
  • In this paper, we try to detect the peel out effect and find the strain difference between the main structure and retrofitting patch material when they separate from each other. In the experiment, two fiber optic Bragg grating sensors are applied to the main concrete structure and the patching material separately at the same position. The sensors show coincident behaviors at the initial loading, but different behaviors after a certain load. The test results show the possibility of optical fiber sensor monitoring of beam structures retrofitted by the composite patches.

  • PDF

A mono-material tactile sensor with multi-sensing properties

  • Shida, Katsunori;Yuji, Junnichiro
    • 제어로봇시스템학회:학술대회논문집
    • /
    • 제어로봇시스템학회 1994년도 Proceedings of the Korea Automatic Control Conference, 9th (KACC) ; Taejeon, Korea; 17-20 Oct. 1994
    • /
    • pp.587-592
    • /
    • 1994
  • To realize artificial device with sensing ability of the human skin, a mono-material tactile sensor with three sensing functions made of some elastic thin electro-conductive rubber sheet with eight latticed patch elements is proposed. This trial sensor provides the information of three kinds of model material characteristics such as thermal property, hardness property and the surface situation of materials by setting up three kinds of surface models as test materials. It can be finally expected to estimate unknown model materials by analyzing the data of the sensor.

  • PDF

Improvement of Communication Reliability of Small UAV by a Tapered Stacked Antenna

  • Kim, Duck-Hwan;Lee, Kyu-Hwan;Kim, Young-Sik
    • ETRI Journal
    • /
    • 제28권6호
    • /
    • pp.796-798
    • /
    • 2006
  • This letter proposes a tapered stacked microstrip antenna for application in small unmanned aerial vehicles (UAVs), which has advantages in mountainous terrains. With its tapered structure and increased bandwidth designed to operate at the resonance frequency of 2.4 GHz, the proposed antenna improves directivity, accuracy, and precision of small UAVs. The test flight results show the proposed tapered antenna has a three times higher impedance capability of 350 MHz based on VSWR<2. The transmission pattern is also more reliable than that of previous antenna designs.

  • PDF

A Study on Repair Technique after Damage of Aircraft Sandwich Composite Structure (항공기 기체에 적용된 샌드위치 복합재 구조의 손상 후 수리 방안 연구)

  • Park, Hyunbum;Kong, Changduk
    • Journal of Aerospace System Engineering
    • /
    • 제7권1호
    • /
    • pp.39-43
    • /
    • 2013
  • In this study, damage assesment and repair technique of aircraft adopted on Sandwich composite structure were performed. The sandwich composite structure were damaged by drop weight type impact test machine. The damaged sandwich composite structure was repaired using external patch repair method after removing damaged area. This study presents comparison results of the experimental investigation between the impact damaged and the repaired specimen.

A novel treatment of nonmatching finite element meshes via MLS approximation with stabilized nodal integration (이동 최소 제곱 근사와 안정화 절점 적분을 이용한 불일치 유한 요소망의 처리)

  • 조영삼;김현규;전석기;임세영
    • Proceedings of the Computational Structural Engineering Institute Conference
    • /
    • 한국전산구조공학회 2002년도 가을 학술발표회 논문집
    • /
    • pp.591-598
    • /
    • 2002
  • The interface element method for non-matching FEM meshes is extended using stabilized nodal integration. Two non-matching meshes are shown to be joined together compatibly, with the aid of the moving least square approximation. Using stabilized nodal integration, the interface element method is able to satisfy the patch test, which guarantees the convergence of the method.

  • PDF

Design of An X-Band Microstrip Array Antenna (X-대역 마이크로스트립 배열 안테나 설계)

  • 윤용민;이석곤;최재현;노진입;김동환;안병철
    • Proceedings of the IEEK Conference
    • /
    • 대한전자공학회 2002년도 하계종합학술대회 논문집(1)
    • /
    • pp.447-450
    • /
    • 2002
  • In this paper, we present design methods for a series-fed microstrip patch array operating at X-band frequency. The array consists of 18 rectangular patches connected to 3 quarter-wave impedance transformers. The power divider is designed for the uniform element excitation. The element excitation is then made to be tapered by increasing the input impedance of elements located at array edges. The designed antenna is fabricated and tested. Results of test show a fair agreement with the prediction.

  • PDF