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http://dx.doi.org/10.15230/SCSK.2013.39.3.215

Use of Oil Red O Staining Method in Non-Comedogenic Test for Cosmetics  

Lee, Sun Hwa (Ellead Co., Ltd.)
Lee, Jung Im (Ellead Co., Ltd.)
Kim, Yoo-Ri (Ellead Co., Ltd.)
Lee, Bum Chun (Ellead Co., Ltd.)
Kang, Min Ji (Department of Dermatology, Inha University College of Medicine)
Choi, Kwang Seong (Department of Dermatology, Inha University College of Medicine)
Moon, Tae Kee (Ellead Co., Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.3, 2013 , pp. 215-224 More about this Journal
Abstract
It has been reported that certain ingredients added to cosmetics clog the skin pores and this can cause outbreaks of comedones which are the primary sign of acne leading to inflammatory acne. This research aims to establish objective evaluation criteria for non-comedogenic cosmetics suitable for acne prone skin. The research has been carried out to examine non-comedogenic test performed in foreign clinical institutions and to establish the evaluation method for detecting comedones outbreaks through repetitive closed back-patch test, Also, usability evaluation on face skin is performed additionally to the same subjects. The analysis of the comedones collected through repetitive closed back-patch test confirmed that the test products, moisturizer and sunscreen product, did not cause comedones. These results had no correlation with the analysis result of the comedones collected from face skin or visual evaluation of acne by Global Acne Grading System (GAGS) in face usability test. Additionally, Oil red O staining was performed on the collected comedones specimen for easy distinction of comedones from hair follicle in image analysis. The analysis result of stained specimen showed higher precision than that of non-stained specimen. This study established a new version of non-comedogenic test for cosmetics, whose objectivity and reliability were improved by inclusion of comedones staining step.
Keywords
acne; acne cosmetics; noncomedogenic; microcomedone; follicular biopsy;
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