• Title/Summary/Keyword: pat

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Selection of Herbicide Resistant Potatoes Transformed with Phosphinothricin Acetyltransferase Gene (Phosphinothricin Acetyltransferase 유전자 도입에 의한 제초제 저항성 감자의 선발)

  • Han, S.S.;Jeong, J.H.;Bang, K.S.;Yang, D.C.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.390-399
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    • 1997
  • This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

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Analysis of Spatial Coincidence of Thrips and Orius sauteri on Greenhouse Eggplants (하우스가지에서 애꽃노린재 Orius sauteri Poppius를 이용한 총채벌레류 방제 및 두 개체군의 공간분포와 공존분석)

  • 송정흡;김수남;이광석;한원탁
    • Korean journal of applied entomology
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    • v.41 no.1
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    • pp.27-32
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    • 2002
  • Biological control of thrips with a predatory bug species, Orius sauteri Poppius, was evaluated in an eggplant greenhouse, To study the biological control strategy of thrips. three Plots were established: Orius sauteri released plot without pesticide treatment (NRT), pesticide treated plot (PAT), and natural enemy removed plot (RNT) The nymphs of O. sauteri were found on the upper side of leaves in NRT at 21 days after the first release of O. sauteri. The density of thrips were dropped and maintained at lower level in NRT than in RNT. Average damage index of NRT, PAT and RNT was 1.35, 1.21 and 2.90 and the percentage of damaged fruit ratio of those was 70.3, 68.6 and 99.0%, respectively. The damage index and percentage of damaged fruit in NRT were slightly higher than those of PAT, but much lower than in RNT. Taylor's band Iwao's $\beta$ were greater than 1 for both populations. indicating aggregated distribution. Spatial coincidence index of O. sauteri was high most of the time even at low densities of both populations. The refuge index for thrips decreased as O. sauteri density increased. Results of this study suggest that O. sauteri is a promising natural enemy for thrips control on eggplant.

Thermal pointing error analysis of the observation satellites with interpolated temperature based on PAT method (PAT 기반 온도장 보간을 이용한 관측위성의 열지향오차해석)

  • Lim, Jae Hyuk;Kim, Sun-Won;Kim, Jeong-Hoon;Kim, Chang-Ho;Jun, Hyoung-Yoll;Oh, Hyeon Cheol;Shin, Chang Min;Lee, Byung Chai
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.1
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    • pp.80-87
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    • 2016
  • In this work, we conduct a thermal pointing error analysis of the observation satellites considering seasonal and daily temperature variation with interpolated temperature based on prescribed average temperature (PAT) method. Maximum 200 degree temperature excursion is applied to the observation satellites during on-orbit operation, which cause the line of sight (LOS) to deviate from the designated pointing direction due to thermo-elastic deformation. To predict and adjust such deviation, the thermo-elastic deformation analysis with a fine structural finite element model is accomplished with interpolated thermal maps calculated from the results of on-station thermal analysis with a coarse thermal model. After verifying the interpolated temperatures by PAT with two benchmark problems, we evaluate the thermal pointing error.

Monitoring of Genetically Modified Soybean and Processed Foods in Korean Market using PCR (PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링)

  • Kim, Myo-Young;Kim, Jae-Hwan;Kim, Hyun-Joong;Park, Sun-Hee;Woo, Geon-Jo;Kim, Hae-Yeong
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.344-347
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    • 2003
  • A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

Realistic Re-lighting System with Occlusion (사실적인 리라이팅 시스템 연구)

  • Park, Jae-Wook
    • Cartoon and Animation Studies
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    • s.38
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    • pp.133-144
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    • 2015
  • Lighting work on 3D animation requires much time and labor. For example, about 60 lighting artists were needed for the theater animation named Postman Pat the Movie globally distributed in 2014. These artists were needed to rapidly respond with shot feedback. However, complicated shots required much of rendering time. Therefore, it was difficult to correspond the work in the middle of test rendering. After 2005, re-lighting techniques were developed to cope with such situations. However, it was not feasible to create shadow with current re-lighting technique which doesn't make natural or realistic result. This paper has realized relighting system that could realistic quality while maintaining fast calculation by applying rasterized ambient occlusion to the relighting calculation of each of the lights. This relighting system has been used for the Hollywood animation titled "Postman Pat: The Movie" globally released in 2014. It is anticipated for this method to be more widely used for animation manufacture that other manufacturers can reduce expenses and create well completed output in the future.

Development of Chair Backrest for Non-intrusive Simultaneous Measurement of ECG and BCG (심전도와 심탄도의 무구속적 동시 측정을 위한 의자 등받이 개발)

  • Lim, Yong-Gyu
    • Journal of the Institute of Convergence Signal Processing
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    • v.19 no.3
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    • pp.104-109
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    • 2018
  • A non-intrusive ECG and BCG measurement system is introduced. The system is built on a auxiliary backrest of a chair. The developed system is aimed to non-intrusive assessment of cardiovascular dynamic indices such as pulse arrival time(PAT) and pre-ejection period (PEP). In the system, capacitive active electrodes and capacitive grounding were used for the non-intrusive indirect-contact ECG measurement, and EMFi pressure sensor was used for the non-intrusive BCG measurement. The capacitive active electrodes and the EMFi sensor were attached on the backrest. Using the system, ECG and BCG were successfully acquired. The measured BCG showed peaks that following ECG R peaks. It was shown that the time interval between Q wave in ECG and first peak in BCG correlates Pre-ejection period measured by impedance-cardiogram. The results showed that the introduced system can be used for the non-intrusive various cardiovascular information including ECG, PAT, PEP.

A Study on Frequency, Type, and Context of Violence in School-Life Webtoon (학원물 웹툰에 나타난 폭력의 양태와 맥락에 대한 내용분석)

  • Kim, Youn-jong;Mun, Anna
    • The Journal of the Korea Contents Association
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    • v.20 no.1
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    • pp.245-258
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    • 2020
  • The study analyzed the frequency, type, and context of violence in 10 school-life webtoon published on Korean portal-site, Naver. Results of content analysis showed 2.15 PAT(Perpetrator-Action-Target) per 1 episode. As for the types of violence, physical violence accounted for 73.2 percent of PATs. As for the characteristics of characters, 53.6 percent of those who committed violence were set to have good-looking. 35.9% of those who committed of violence were heros and 37.3% were villains. The case that perpetrator and target were friends accounted for 60.8%. The case that a travesty is made of the violent scenes adopting excessive expressions, overtures, and balloons accounted for 66.7%. The most common motive for violence was the means for the interests and beliefs of individuals and groups (29.4%), followed by fun (20.9%). The case that the punishment or compensation for violence is absent accounted for 79.9%.

Analysis on Peritext of the Picture-book 『The Legend of Pat-bing-su』 (그림책 『팥빙수의 전설』 페리텍스트의 서사적 의미 분석)

  • A Reum Nam;Sang Lim Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.185-193
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    • 2023
  • The purpose of the study was to analyze the narrative meaning of the peritext in the picture-book of 『The Legend of Pat-bing-su』. For the purpose, based on the narrative components proposed by Nam and Kim, the narrative meanings of the peritext were analyzed. As the results, the peritexts of 『The Legend of Red Pat-bing-su』 include basic information of the title, author's name, and publication information, and physical elements of hard cover binding with matte rectangular paper that matches the narrative, which support prior understanding of the narratives. In addition, the peritext components such as covers, endpapers, title page, and copyright page lead readers to predict or expand narratives components to predict, expand, or transform the narrative, and provide additional information for understanding plots or genres.

Polymorpshisms of XPC Gene and Risk of Primary Lung Cancer in Koreans (한국인에서 XPC 유전자의 다형성과 원발성 폐암의 위험도)

  • Kim, Kyung-Rock;Lee, Su-Yeon;Choi, Jin-Eun;Kim, Kyung-Mee;Jang, Sang-Soo;Jung, Chi-Young;Kang, Kyung-Hee;Jeon, Kyung-Neoyh;Cha, Sung-Ick;Kim, Chang-Ho;Kam, Sin;Jung, Tae-Hoon;Park, Jae-Yong
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.2
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    • pp.113-126
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    • 2002
  • Background : DNA repair plays a crucial role in protection from cancer-causing agents. Therefore, a reduced DNA repair capacity can increase the susceptibility to lung cancer. The XPC gene contains 15 exons and encodes a 940 amino acid protein that plays a central role in DNA damage recognition of the nucleotide excision repair pathway, which is a major DNA repair mechanisim removing the bulky-helix distorting DNA lesions caused by smoking. Recently several polymorphisms in the XPC gene were identified. In addition, it is possible that these polymorphisms may affect the DNA repair capacity, which modulate cancer susceptibility. The relationship between codon 499 and 939 polymorphisms, and a poly(AT) insertion/deletion polymorphism in the XPC gene, and the lung cancer risk were investigated. Materials and Methods : The genotypes were determined using either PCR or PCR-RFLP analysis in 219 male lung cancer patients and 150 healthy males controls. Results : The frequencies of the genotypes (Val499Ala, PAT and Lys939Gln) among the cases were not significantly different from those of the controls. There was no significant associantion between these polymorphi는 and the lung cancer risk when the analyses were stratified according to age, smoking status and the pack-years of smoking. Moreover, the genotypes had no apparent relationship with any of the histological types of lung cancer. There was a linkage disequilibrium among the Val499Ala, PAT and Lys939Gln polymorphisms. The PAT polymorphism had a strong linkage disequilibrium with the Lys939Gln polymorphism (kappa value=0.87). The XPC haplotypes showed no significant association with the lung cancer risk. Conclusion : These results suggest that XPC Val499Ala, PAT and Lys939Gln polymorphisms are not major contributors to the individual lung cancer susceptibility in Koreans.

Principles and Medical Applications of Biomedical Photoacoustic Tomography (의료용 광음향 단층촬영 원리와 의학적 응용)

  • Song, Chul-Gyu;Ryu, Sang-Hun;Kim, Do-Hyun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.60 no.6
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    • pp.1209-1214
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    • 2011
  • Photoacoustics has been broadly studied in biomedicine, for both human and small animal tissues. Photoacoustics uniquely combines the absorption contrast of light or radio frequency waves with ultrasound resolution. Moreover, it is non-ionizing and non-invasive, and is the fastest growing new biomedical method, with clinical applications on the way. This paper provides a brief recap of recent developments in photoacoustics in biomedicine, from basic principles to applications. The emphasized areas include the new imaging modalities as well as translational research topics. A primary PA application in biomedicine is photoacoustic tomography (PAT). The past decade has seen fast developments in both theoretical reconstruction algorithms and innovative imaging techniques, and PAT has been implemented in imaging different tissues, from centimeter-large breast tumors to several micrometer-large single red blood cels (RBC). PAT now provides structural, functional and molecular imaging. Overall, PA techniques for biomedicine are maturing. They have been widely used to study both animal and human tissues. Recently, more and more research focuses on clinical applications. Commercialized PA systems are expected to be available in the near future, and wide clinical PA applications are foreseen.