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A study on the Development of Standardized Recipe and the Microbiological Assessment and Sensory Evaluation of Korean Traditional Starch Foods for Steam Convection Oven and Cook/Chill System for Kindergarten Foodservice Operations (유치원 급식에 적용하기 위한 한국의 전통적인 전분류 음식의 Steam Convection Oven 및 Cook/Chill System용 레시피 개발 및 미생물적, 관능적 품질 평가에 대한 연구)

  • 강현주;김경자;김은희
    • Korean journal of food and cookery science
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    • v.14 no.4
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    • pp.348-357
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    • 1998
  • To meet the demand for high quality of foods with the expanded implementation of foodservice into kindergarten, and to make the efficient use of resources, the necessity of introducing central production unit with cook/chill system into kindergarten foodservice is getting increased. The purpose of this study were to develop standardized recipe applicable to cook/chill system for kindergarten foodservice and to evaluate the microbial and sensory quality. For quantitty production of cook/chill system in kindergarten foodservice, Korean traditional starch foods were selected as menu items. The followings are summary of the results: 1. Standard recipes to produce Various Boiled Rice (Boriebap and Yackbap) and Rice Cake (Chapsal-pat-sirutuk) for cook/chill system have been developed. 2. The microbial quality of Boribap, Yackbap and Chapsal-pat-sirutuk during 10 days chilled storage was very excellent. And in this experiment, the stability of storage of standard recipes of Boribap, Yackbap and Chapsal-pat-sirutuk at cook/chill system was proved for 10 days. 3. In the result of sensory evaluation, apperance of Boribap was decreased significantly on the third day of storage (p<0.005). The rest of the item of sensory evaluation of Boribap has no significance between the first day and the third day of storage. The tenderness (p<0.05) and gelatinousness (p<0.005) of Yackbap had been rated high significantly in the third day of storage than the first day of storage. The rest of the item of sensory evaluation of Boribap has no significance between the first day and the third day of storage. The color and chewiness of Chapsal-pat-sirutuk was decreased significantly on the third day of storage (p<0.05). The rest of the item of sensory evaluation of Chapsal-pat-sirutuk has no significance between the first day and the third day of storage.

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Anatomical Studies on the Classification of Cultivated Peony in Korea (재배작약의 분류에 관한 해부학적연구)

  • Yu, Seung-Jo
    • Korean Journal of Pharmacognosy
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    • v.1 no.3
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    • pp.81-92
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    • 1970
  • So far, the cultivated peony is known to be originated from an indigenous species, Paeonia albiflora $P_{ALLAS}$ var. trichocarpa $B_{UNGE}$ (PAT). In this study, these two species were morphologically examined in the external and internal feature and in the pattern of callus formation by tissue culture. Also, they were compared with another indigenous species, P. japonica $M_{IYABE}$ et $T_{AKEDA}$ var. pilosa $N_{AKAI}$ (PJ), which were regarded as being scarcely related to them. The root of the cultivated peony is massive consisting with several storage roots, each of them is a hypotrophic and fusiform. The root of PAT consists of several storage roots, each of them is branching and slender. And the storage root of PJ is short, bended buried horizontally, protruding a number of corpulent lateral root. The secondary xylem of the cultivated peony is small clusters of vessels and xylem fibres are arranged in scalariform and among these cluster, single vessel is joined, but that of PAT is small clusters of vessels are arranged in separate scalariform but are not connected with each other and that of PJ is vessels and xylem fibers are grouped together in elongated clusters that radiate outward from the center. Protoxylem of the cultivated peony is surrounded by four large metaxylem, but that of PAT and PJ by seven. On the other hand, the callus formation patterns of these peonies were different; the cultivated peony callus is formed in an orderly fashion by the mammalate meristematic cell groups, PAT callus is in disorder by the meristematic cells arranged in linear, and PJ callus is in order by the meristematic cells arranged in linear. By the comparison of three different plants in the anatomical appearance and the callus formation pattern, it is evident that the cultivated peony is not derived from PAT.

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Effects of Minute Pirate Bug, Orius strigicollis (Hemiptera: Anthocoridae) on Control of Thrips on Hot Pepper in Greenhouse (하우스고추에서 Orius strigicollis Poppius를 이용한 총채벌레류 방제효과)

  • 송정흡;강상훈;이광석;한원탁
    • Korean journal of applied entomology
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    • v.40 no.3
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    • pp.253-258
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    • 2001
  • Biological control of thrips with Orius strigicollis Poppius was evaluated in a hot pepper greenhouse. To study the biological control strategy of thrips on hot pepper grown in greenhouse, three plots were established: natural enemy removed plot(CNT), pesticide treated plot(PAT) and O. strigicollis released plot with no pesticide application (NRT). The nymphs of O. strigicollis were found on the leaves of lower stratum in CNT and NRT at 13 days after the first release. The density of thrips was suddenly dropped and maintained at low level since 15 day after transplanting in NRT. The densities of thrips on hot pepper flowers was also maintained at much less level in NRT than in CNT. The thrips and O. strigicollis, which were aggregated in flowers, may have resulted in improved predator searching. Average damage indices of CNT, PAT and NRT were 0.31, 0.05 and 0.08 and the percentage of damaged fruit were 80.0, 17.1 and 24.8% respectively. The damage index and percentage of damaged fruit of NRT was slightly higher than PAT, but very lower than CNT. The introduction of the adults of O. strigicollis, which was the biological control agent for the control of thrips, was effective on hot pepper.

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Development of Process Analytical Technology (PAT) for Total Quality Innovation on Pharmaceutical Processes (의약품 제조공정에서의 전사적 품질혁신을 위한 공정분석기술 개발)

  • Shin, Sang-Mun;Park, Kyung-Jin;Choi, Yong-Sun;Lee, Sang-Kil;Choi, Guang-Jin;Kwon, Byung-Soo;Cho, Byung-Rae
    • Journal of Pharmaceutical Investigation
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    • v.37 no.6
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    • pp.329-338
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    • 2007
  • The quality assurance issue of drug products is more important than the general product because it is highly related to the human health and life. In this reason, the regulatory guide lines have continuously been intensified all around the world. In order to achieve effective quality assurance and real-time product release (RTPR) of drug products, process analytical technology (PAT), which can analyze and control a manufacturing process, has been proposed from the United States. With the PAT process, we can obtain significant process features of materials, quality characteristics and product capabilities from a raw material to the final product in the real-time procedure. PAT can also be utilized to process validation using information system that can analyze the risk of drug products through out an entire product life-cycle. In this paper, we first offered a new concept for the off-line process design methods to prepare the improved quality assurance restrictions and a real-time control method by establishing an information system. We also introduced an automatic inspection system by obtaining surrogate variables based on drug product formulations. Finally, we proposed an advanced PAT concept using validation and feedback principles through out the entire life-cycle of drug product manufacturing processes.

Effects of Chronic Treatments with 5-HT Uptake Inhibitors on the [$^3H$]Imipraine and [$^3H$]Paroxetine Binding, [$^3H$]5-HT Uptake, and 5-HT Content of the Rabbit Platelet (5-HT 흡수억제성 항우울제들이 가토혈소판의 [$^3H$]Imipramine과 [$^3H$]Paroxetine Binding, [$^3H$]5-HT 흡수, 및 5-HT함량에 미치는 영향)

  • Won, Kyong-Sik;Lee, Min-Soo;Shin, Kyung-Ho;Chun, Boe-Gwun;Kwak, Dong-Il
    • Korean Journal of Biological Psychiatry
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    • v.1 no.1
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    • pp.88-97
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    • 1994
  • Many evidences are compatible with the correlation between the inhibition of [$^3H$] imipramine([$^3H$]IMI) and [$^3H$]paroxetine([$^3H$]PAT) binding to the 5-hydroxytryptamine(5-HT) transporter complex and the 5-HT uptake of 5-HT neurons and platelets, and most antidepressants have been shown to inhibit the [$^3H$]IMI and [$^3H$]PAT binding and the neuronal 5-HT uptake. However, several paradoxical research findings led to doubt about the pharmacological significance of the [$^3H$]IMI and [$^3H$]PAT binding sites. This study was carried to clarify the correlation between the [$^3H$]IMI and [$^3H$]PAT binding parameters and the tissue 5-HT content or/and [$^3H$]5-HT uptake in the rabbit platelet, which contains 40 times ad much 5-HT as that of human platelet and shows the 10 fold higher $B_{max}$ of the 5-HT transporter binding to a 5-HT uptake inhibitor. The rabbits were treated for 28 days with amitriptyline(4mg/kg/day : AP), fluoxetine(0.5mg/kg/day : FO), and sertraline(0.5mg/kg/day : SA) via an Alzet osmotic pump implanted for constant infusion. The [$^3H$]IMI binding $B_{max}$ and $K_d$ of the rabbit platelets were $6.4{\pm}1.2$pmol/mg protein and $10.9{\pm}2.1$nM and those in the [$^3H$]PAT binding were $8.6{\pm}1.1$pmol/mg protein and $1.6{\pm}0.3$nM, respectively. AP slightly increased $B_{max}$ of [$^3H$]IMI binding and both [$^3H$]IMI binding and [$^3H$]PAT binding $K_d$, and i contrast, it slightly decreased $B_{max}$ of [$^3H$]PAT binding. FO Slightly increased $K_d$ of both and [$^3H$]IMI and [$^3H$]PAT binding and slightly decreased $B_{max}$ of [$^3H$]IMI and [$^3H$]PAT binding. SA produced the significant increase of [$^3H$]PAT binding $B_{max}$ and the slight increase of both [$^3H$]IMI and [$^3H$]PAT binding $K_d$ and in contrast, it slightly decreased $B_{max}$ and of [$^3H$]IMI binding. And, the $V_{max}$ and $K_m$ of platelet [$^3H$]5-HT uptake were $24.2{\pm}2.4$pmol/$10^8$ platelets/min and $3.3{\pm}0.3$nM, respectively. The $V_{max}$ was little affected by AP, FO, or SA, but the [$^3H$]5-HT uptake $K_m$ value was moderately increased by FO. However, the platelet 5-HT content was moderately decreased by all of the 5-HT uptake inhibitors used in this study. These results seem to be consistent with the allosterical and competitive interaction of 5-HT uptake inhibiting antidepressants with each other as well as 5-HT in the 5-HT transporter binding, and provide no support for the view that the potencies of 5-HT uptake inhibitors to inhibit the [$^3H$]IMI or [$^3H$]PAT binding with 5-HT transporter complex correlate with their antidepressant potencies.

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Development of glufosinate-tolerant GMO detection markers for food safety management (식품안전관리를 위한 제초제 glufosinate 특이적 GM 작물 검출마커 개발)

  • Song, Minji;Qin, Yang;Cho, Younsung;Park, TaeSung;Lim, Myung-Ho
    • Korean Journal of Food Science and Technology
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    • v.52 no.1
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    • pp.40-45
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    • 2020
  • Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

Transformation of Populus alba $\times$Populus glandulosa Using Phosphinothricin Acetyltransferase Gene (Phosphinothricin acetyltransferase 유전자를 이용한 현사시의 형질전환)

  • 오경은;양덕춘;문흥규;박재인
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.163-169
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    • 1999
  • This study was conducted to produce herbicide resistant plants by transferring phosphinothricin acetyltransferase (PAT) gene into Populus alba $\times$ Populus glandulosa No .3 using Agrobacterium tumefaciens MP 90/PAT. Leaf segments from in vitro grown shoots of hybrid poplar No. 3 were soaked in a AB medium containing Agrobacterium tumefaciens MP 90/PAT for 10 min and cocultivated for 2 days on MS medium containing 1.0 mg/L 2,4-D and 0.2mg/L kinetin (CIM). Putative transformed calli could be selected after cocultivation of leaf segments on CIM supplemented with 50mg/L kanamycin and 500mg/L cefotaxime for 3 weeks. The selected calli were cultured on CIM supplemented with 50 mg/L kanamycin and 500 mg/L cefotaxime for 5~8 weeks before transfer to WPM containing 1.0mg/L zeatin, 0.1mg/L BAP, 50 mg/L kanamycin and 500mg/L cefotaxime for shoot regeneration. Shoots were regenerated from the callus after 4 week cultivation, and the regenerants were grown on the same medium for 7~l0 weeks. The plants rooted on 1/2 WPM containing 0.2 mg/L IBA and 50 mg/L kanamycin. To confirm the gene insertion into plants, GUS activity was detected by histochemical assay in the transformed plants. Finally, the presence of both NPT II and PAT genes from the transgenic plants were confirmed by PCR amplification with the gene specific primers and subsequent PCR-Southern blot with DIG-labeled PAT gene probe. After acclimatization in pots for 4 weeks, the plants were sprayed by 3 mL/L of Basta to test resistance to the herbicide. The transgenic plants remained green, whereas all the control plants died after one week.

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Robust Detection Technique for Abandoned Objects to Overcome Visual Occlusion (시각적 가려짐을 극복하는 강인한 유기물 탐지 기법)

  • Kim, Won
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.10 no.6
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    • pp.23-29
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    • 2010
  • Nowadays it is required to design intelligent visual surveillance systems which automatically detect abandoned objects in public places to strengthen the social safety. Already recognized abandoned objects can be occluded partially or fully by surrounding people in public places after the first recognition. To improve an essential recognition performance index PAT, the system should overcome the occlusion problems. In this research, a design scheme is newly proposed to construct the robust detection system which is comprised of multiple stages considering the occlusion problem. To show the feasibilities of the proposed system, the evaluation was tried for the prepared image streams including 6 various situations and the experimental results show 96% and 75% in PAT performance for intrusion and abandoning events, respectively. Finally in spite of full occlusions by multiple persons, the proposed system shows the capability to continuously recognize the abandoned object after complex occlusions disappear.

Non-intrusive measurement of pulse arrival time and Estimation of Systolic Blood Pressure (무구속적 맥파 전달 시간의 측정을 통한 혈압 추정)

  • Chee, Young-Joon;Park, Kwang-Suk
    • Proceedings of the IEEK Conference
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    • 2005.11a
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    • pp.489-492
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    • 2005
  • Even though the blood pressure is one of the most widely used index for the healthcare monitoring of hypertensive and normotensive persons, there is no non-intrusive measurement method which is commercialized until now. Pulse Arrival Time (PAT) is known that it has close relation with the systolic blood pressure (SBP) and arterial stiffness. In this study, SBP estimation methods by non-intrusive measurement of PAT are suggested. For the unconstrained measurement of PAT, the first method used the electrically non contact electrocardiogram (ENC-ECG) technique and the reflective type of Photoplethysmography (PPG) sensor on the computer mouse. In the second method, ENC-ECG and the air pressure sensor in the seat cushion on a chair were measured. The third method used ECG electrodes and PPG sensors on the toilet seat cover. The validation and regression analysis of the relationship of PAT and SBP are summarized. These methods have considerable errors to be used for all people. But these can be applied for each subject after the parameter customization within acceptable error. So, it is feasible for suggested methods to be used for monitoring of SBP in daily life in non-intrusive way when there is personal identification system of each subject.

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Naive Bayes Learner for Propositionalized Attribute Taxonomy (명제화된 어트리뷰트 택소노미를 이용하는 나이브 베이스 학습 알고리즘)

  • Kang, Dae-Ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2008.10a
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    • pp.406-409
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    • 2008
  • We consider the problem of exploiting a taxonomy of propositionalized attributes in order to learn compact and robust classifiers. We introduce Propositionalized Attribute Taxonomy guided Naive Bayes Learner (PAT-NBL), an inductive learning algorithm that exploits a taxonomy of propositionalized attributes as prior knowledge to generate compact and accurate classifiers. PAT-NBL uses top-down and bottom-up search to find a locally optimal cut that corresponds to the instance space from propositionalized attribute taxonomy and data. Our experimental results on University of California-Irvine (UCI) repository data sets show that the proposed algorithm can generate a classifier that is sometimes comparably compact and accurate to those produced by standard Naive Bayes learners.

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