• Title/Summary/Keyword: partial weight

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Effect of Partial Oxygen Pressure on the Growth and Defense Enzyme Activities of Streptomyces coelicolor in continuous culture system (Streptomyces coelicolor의 연속 배양시 산소 분압에 따른 방어 효소의 활성 변화)

  • 박용두;이계준;노정혜
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.538-543
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    • 1994
  • Effect of partial oxygen pressure on the cell growth and the activities of oxidative defense enzymes were measured in the continuous culture of Streptomyces coelicolor. Both the wild type and the mutant strain resistant to hydrogen peroxide were cultured and the dry cell weight of the two cultures were measured at different oxygen tensions. Growth of the wild type was inhibited by oxygen at above 0.5 vvm. Growth of the hydrogen peroxide resistant mutant was stimulated by pure oxygen at 0.5 vvm but was inhibited by oxygen at 1.0 vvm. Therefore, growth of the hydrogen peroxide resistant mutant was less affected by the deleterious oxidative stress of oxygen. Activities of the several defense enzymes were also measured at different oxygen tensions. Activities of catalase and glucose-6-phosphate dehydrogenase increased significantly as oxygen pressure increased in the wild type culture. In the mutant, however, increase in those enzyme activities was not obvious whereas the uninduced levels of the above enzymes were higher than those of wild type. As judged by Western blotting, the amount of the major catalase increased as the oxygen pressure increased. This indicates that the induction of the catalase activity by oxygen pressure is mostly due to the increase in the expression level for the major catalase.

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Failed Airway Management in a Patient with Wound Hematoma After Partial Mandibulectomy and Reconstruction with Free Flap (하악골 부분절제술을 받은 환자에서 발생한 기도 관리 실패)

  • Kim, Seokkon;Song, Jaegyok;Kang, Bongjin;Choi, Cheolwhan;Choi, Gyuwoon
    • Journal of The Korean Dental Society of Anesthesiology
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    • v.13 no.3
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    • pp.127-131
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    • 2013
  • We experienced failed airway management in a patient who had partial mandibulectomy and reconstruction with free-flap. 40 year-old man (height: 164 cm, body weight: 59 kg) with malignant melanoma on #38 tooth area of mandibular body was scheduled for partial mandibulectomy and reconstruction with free flap. Approximately fifteen-hours after surgery, the patient was extubated without complication. Seven hours after extubation, we experienced respiratory failure andfailed airway managementdue to airway edema and neck. We failed orotracheal intubation with direct laryngoscopy andlaryngeal mask airway, thus we tried tracheostomy but the patient was hypoxic state for more than 30 minutes. The patient had got hypoxic brain damage in whole cerebral cortex and basal ganglia. We should have the policy of airway management of the patients who have massive oro-maxillo-facial surgery and all medical personnel who treat these patients should be educated the policy and airway management methods.

Effect of crushed waste glass as partial replacement of natural fine aggregate on performance of high strength cement concrete

  • Ajmal, Paktiawal;Mehtab, Alam
    • Advances in materials Research
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    • v.11 no.4
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    • pp.251-277
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    • 2022
  • Disposal of industrial waste in cities where municipal authorities permitting higher floor area ratio coupled with increasing living standards, a lot of demolition waste is being generated. Its disposal is a challenge particularly in megacities where no landfills are available. The ever-increasing cost of building construction materials also necessitates consuming demolition wastes in a useful manner to save fresh natural raw materials. In the present work, the crushed waste glass is used in high-strength concrete as a partial replacement of fine aggregate. The control concrete of grade M60 was proportioned following BIS 10262-2009. The crushed waste glass has been used as a partial replacement with varying percentages of 10, 20, 30, and 40% by weight of fine aggregate. Experimental tests were carried on the fresh and hardened state of the concrete. The effect of crushed waste glass on the workability of the concrete has been investigated. Non-destructive tests, acid attack tests, compressive strength, split tensile strength, and X-ray diffraction analysis was carried out for the control concrete and concrete containing crushed waste glass after 7, 28, and 270 days of normal curing. The results show that for the same w/c ratio, the workability of concrete increases with increasing replaced crushed waste glass content. However, the decrease in compressive strength of the concrete after 28 days of normal curing and further after 28 days of acid attacks, up to 30% replacement level of fine aggregate by the crushed waste glass is insignificant.

Partial Purification and Properties of Polygalacturonase Produced by Botrytis cinerea (잿빛곰팜이병균 Botrytis cinera가 분비하는 Polygalacturonase의 부분정제와 특성)

  • 나유진;김재원;정영륜;허남응;조광연
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.215-221
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    • 1994
  • Polygalacturonase (PG) produced by Botrytis cinerea in the culture broth containing citrus pectin as a carbon source was partially purified and characterized. PG was produced on a range of carbon sources such as starch, glycerol, cellobiose, and Na+-PAG with total activities of 34.8, 32.0, 29.2, 27.8 units, respectively. The specific activity was highest with 2316.7 units on Na+-PGA. Proteins of culture filtrate were concentrated with polyethylene glycol and acetone and applied to a hydroxyapatite column. Among three active fractions collected from the column, the reaction containing the highest PG activity was resolved by a Q-sepharose column. The active fraction from the Q-sepharose column was further purified by HPLC Mono Q column. The partially purified enzyme was analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Among a few protein bands revealed, the amount of the protein of which molecular weight estimated to be 43 kDa coincided with the PG activity. The partially purified PG had optimal temperatures between 35~55$^{\circ}C$ and pH between 4.5~5.5.

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Production, Partial Purification and Physico-Chemical Characteristics of Laccase from Pleurotus ostreatus (Pleurotus ostreatus가 생산하는 Laccase의 부분정제 및 효소적 특성)

  • Lee, Jae Sung;Lee, Un Jung;Suh, Dal Sun
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.65-70
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    • 1985
  • The production media, partial purification and the enzymatic characteristics of laccase from Pleurotus ostreatus were investigated. Among various media tried the double strength onion media showed much higher production of laccase compared to glucose and/or CMC added media. The laccase from Pleurotus ostreatus has the optimum pH of 5.94 for the activity and appears to be stable at relatively broad pH spectrum (4.7-8.7). The experiments on the temperature stability shows that above 90% activity could be preserved after holding at $30^{\circ}C$ for 40 min., 60% activity was remained after 40 min. at $50^{\circ}C$. The Km value of the laccase from Pleurotus ostreatus was estimated to be 3.209mM and the molecular weight of laccase was estimated to be 55,000 by SDS-Polyacrylamide gel electrophoresis.

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MULTIPLE SOLUTIONS TO DISCRETE BOUNDARY VALUE PROBLEMS FOR THE p-LAPLACIAN WITH POTENTIAL TERMS ON FINITE GRAPHS

  • CHUNG, SOON-YEONG;PARK, JEA-HYUN
    • Bulletin of the Korean Mathematical Society
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    • v.52 no.5
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    • pp.1517-1533
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    • 2015
  • In this paper, we prove the existence of at least three nontrivial solutions to nonlinear discrete boundary value problems $$\{^{-{\Delta}_{p,{\omega}}u(x)+V(x){\mid}u(x){\mid}^{q-2}u(x)=f(x,u(x)),x{\in}S,}_{u(x)=0,\;x{\in}{\partial}S}$$, involving the discrete p-Laplacian on simple, nite and connected graphs $\bar{S}(S{\cup}{\partial}S,E)$ with weight ${\omega}$, where 1 < q < p < ${\infty}$. The approach is based on a suitable combine of variational and truncations methods.

Adaptive MAC Scheduling Scheme using Partial Queue Length Information in IEEE Wireless LAN (IEEE 무선랜에서 부분적인 큐 정보를 이용한 적응적인 MAC 스케쥴링 기법)

  • Shin Soo-Young;Jang Yeong Min
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.11B
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    • pp.974-979
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    • 2004
  • In the IEEE 802.1 Ib, DCF provides contention based services and PCF provides contention free services for QoS suppof. DCF uses CSMA/CA (Carrier Sense Multiple Access/collision Avoidance) as an access protocol. In this paper, an enhanced PCF, which gives weights to channels with heavy traffic load, was proposed. The weight is depending on the partial queue length information from STAs. NS-2 simulation results show that the proposed scheme is an promising approach for enhancing the PCF.

A Study on the Prevention of Crack Generated in Interface Cu and Epoxy with Painting of Carbon (카본 도포에 따른 Cu-Epoxy 접촉면에서 발생하는 크랙방지에 관한 연구)

  • 송재주;김성훈;황종선;박종광;한병성
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.14 no.7
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    • pp.578-583
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    • 2001
  • The bushing for high voltage and large and power should endure weight of itself and force of pushing from contact with circuit breaker. Like this, epoxy mold bushing has to be strong without fault. However, the external circumstances and internal factors was caused by partial discharge, flashover and dielectric breakdown. Therefore, to remove external factor of defect and to prevent the internal cracks and cavity generated from the contraction on interface of Cu-Epoxy, we should form semi-conductive layer on Cu bar by carbon. Then, the PD properties and the insulation qualities of epoxy mold type bushing was able to improved by roles of cushions for the direction of diameter and by effects fo natural sliding like as separated from conductor for the direction of length. So, in this work, we could prove the method of semi-conductive layer in making the long conductor.

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A Covariate-adjusted Logrank Test for Paired Survival Data

  • Jeong, Gyu-Jin
    • Communications for Statistical Applications and Methods
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    • v.9 no.2
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    • pp.533-542
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    • 2002
  • In this paper, a covariate adjusted logrank test is considered for censored paired data under the Cox proportional hazard model. The proposed score test resembles the adjusted logrank test of Tsiatis, Rosner and Tritchler (1985), which is derived from the partial likelihood. The dependence structure for paired data is accommodated into the test statistic by using' sum of square type' variance estimators. Several weight functions are also considered, which produce a class of covariate adjusted weighted logrank tests. Asymptotic normality of the proposed test is established and simulation studies with moderate sample size show the proposed test works well, particularly when there are dependence structure between treatment and covariates.

INHIBITORY EFFECTS OF RED GINSENG EXTRACT ON PRENEOPLASTIC NODULE FORMATION IN THE RAT LIVER OBSERVED BY RAPID BIOASSAY OF CARCINOGENESIS

  • Kim, Yun-Bae;Lee, Yong-Soon
    • Toxicological Research
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    • v.4 no.2
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    • pp.159-179
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    • 1988
  • This study was carried out to investigate the inhibitory effects of red ginseng extract on preneoplastic nodule formation in the rat liver using a rapid bioassay of hepatocarcinogenesis. Wistar male rats in test groups were fed diets containing 3.0, 0.3 or 003% of red genseng extract for 6 weeks after 2 week of dosing with diethylnitrosamine (DEAN, 200 mg/kg B.W., I.P.) or saline solution. The rats in the control groups were treated with DENA or saline alone. Three weeks after the beginning of the experiment, the two-thirds partial hepatectomy was performed on all animals. The inhibitory effects were investigated by measuring the number, total area and size of hyperplastic nodules enhanced by the 2/3 partial hepatectomy following induction with DENA. The results obtained were summarized as follows: 1. In general, the body weight gains of the rats fed diets containing red ginseng extract were higher than those of other groups.

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