• Parasites, Hosts and Diseases
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• v.52 no.6
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• pp.691-694
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• 2014

The purpose of this study was to conduct a survey of Dirofilaria immitis infection among stray cats in Korea using nested PCR. We included 235 stray cats (121 females and 114 males) and evaluated each for the presence of feline heartworm infection. Blood samples were collected from 135 cats in Daejeon, 50 cats in Seoul, and 50 cats from Gyeonggi-do (Province). Of the 235 DNA samples, 14 (6.0%) were positive for D. immitis. The prevalence of infection in male cats (8/114, 7.0%) tended to be higher than that in female cats (6/121, 5.0%), but the difference was not statistically significant. In each location, 8, 2, and 4 cats were positive for infection, respectively, based on DNA testing. No significant differences in the prevalence were observed among the geographic regions, although the rate of infection was higher in Gyeonggi-do (8.0%) than Daejeon (5.9%) and Seoul (4.0%). We submitted 7 of the 14 D. immitis DNA-positive samples for sequencing analysis. All samples corresponded to partial D. immitis cytochrome c oxidase subunit I gene sequences with 99% homology to the D. immitis sequence deposited in GenBank (accession no. FN391553). To the best of our knowledge, this is the first survey using nested PCR to analyze the prevalence of D. immitis in stray cats in Korea.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.94-99
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• 2005

Sixty four bacterial colonies which were able to oxidize the manganese were isolated from soil samples in Mokcheon and Ochang area. Among them, one bacterial strain was selected for this study based on its higher manganese oxidation, and this selected bacterial strain was identified as Aeromonas sp. MN44 through physiological-biochemical test and analysis of its 16s rRNA sequence. Aeromonas sp. MN44 was able to utilize lactose but did not utilize various carbohydrates as a sole carbon source. Aeromonas sp. MN44 showed a very sensitive to antibiotics such as kanamycin, chloramphenicol, ampicillin, tetracycline and spectinomycin, and heavy metal such as cadmium. But this strain showed a high resistance up to mg/ml unit to heavy metals such as lithium and manganese. Optimal manganese oxidation condition of Aeromonas sp. MN44 was pH 7.4 and manganese oxidation activity was inhibited by proteinase K and boiling treatment. So, we concluded that this factor was protein. The manganese oxidizing factor produced by Aeromonas sp. MN44 was partial purified by ammonium sulfate precipitation, DEAE-Toyopearl 650M ion exchange chromatography and Sephadex gel filtration chromatography. Its molecular mass was about 113 kDa.

• 제어로봇시스템학회:학술대회논문집
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• 1999.10a
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• pp.73-78
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• 1999

This paper presents an effective construction method of adaptive multiple control systems utilizing some knowledge upon the plants. The adaptive multiple control system operates plants un-der widely changing environmental conditions. The adaptive multiple control system is composed of a family of candidate controllers together with a supervisor. The system does not require any identification schemes of environmental conditions. Monitoring outputs of the plant, the supervisor switches from one candidate controller to another, The basic ideas of adaptation are as follows: (1)each candidate controller is prepared for each environmental condition in advance; (2)the supervise. applies a sequence of speculative controls to the plant with candidate controllers just after the start of control or just after the detection of a change in the environmental condition. Each candidate controller can keep the system stable during one-step period of the speculative control and the most appropriate candidate controller for the environmental condition to which the system is exposed can be selected before the last trial of speculative control step comes to an end. We proposed a construction method of adaptive multiple control system without any knowledge of plant dynamics and applied the method to a cart-pole balancing problem and a vehicle anti skid braking system. In real applications, as we can often easily obtain a piece of knowledge upon plant dynamics beforehand, we intend to extend the method such that multiple control systems can be efficiently designed using the knowledge. We apply the new idea to the cart-pole balancing problem with variable length of the pole. The simulation experiments lead us to the conclusion that the new attempt can reduce the manpower to design the candidate controllers for adaptive multiple control systems.

• Journal of the Ergonomics Society of Korea
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• v.31 no.6
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• pp.733-740
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• 2012

Objective: The present study systematically analyzed the characteristics of ergonomic layout optimization methods by a comprehensive literature survey. Background: Although layout design methods for ergonomic placement of controls and displays on a console have been developed, understanding of their characteristics is lacking. Method: The present study analyzed layout optimization papers published past 20 years from the following four aspects: optimization model, optimization algorithm, design principle, and constraint/assumption. Results: The existing layout optimization methods based on various optimization techniques consider only a partial set of four layout principles(importance, frequency of use, sequence of use, and functional grouping) and two ergonomic criteria(visibility and reach). In addition, the existing methods oversimplify components in various sizes, shapes, and angles by assuming the equality of the components in size and shape. Conclusion: A more effective layout optimization method is needed which considers the layout principles and ergonomic criteria in a comprehensive manner and reflect the diversity of components in size and shape. Application: The identified characteristics on the existing layout optimization methods can be applicable to development of a better ergonomic console layout design method.

• The Plant Pathology Journal
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• v.22 no.2
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• pp.155-160
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• 2006

A strain of Pepper mottle virus (PepMov) was isolated from chili pepper plants in Korea. In host range study, this virus, designated PepMoV-SNU1, shared most characteristics with PepMoV isolates reported previously. Thermal inactivation point ($45^{\circ}C\;to\;75^{\circ}C$) and dilution end point ($10^{-1}\;to\;10^{-4}$) of PepMoV-SNU1 showed differences depending on the propagation hosts. Cylindrical and pinwheel-shaped inclusions were always observed in pepper leaf tissues infected with the virus alone. Unexpectedly, a special structure of pinwheel shaped inclusion surrounded with unknown small spots was also observed in the leaf section when co-infected with a strain of pepper mild mottle virus. The partial sequence of coat protein gene and 3' untranslated region of PepMoV-SNU1 showed 98% identity with those of other PepMoV isolates. A primer pair derived from 3' end of the coat protein gene and poly A tail regions were designed. Optimal detection condition of PepMoV-SNU1 by RT-PCR was tested to determine appropriate annealing temperature and additional volumes of oligo-dT (18-mer), dNTP, and Taq polymerase. Under the optimized condition, an expected 500 Up PCR-product was detected in pepper leaves infected with PepMoV-SNU1 but not in healthy plants.

• Tunnel and Underground Space
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• v.27 no.2
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• pp.109-119
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• 2017

A lot of twin tunnels were modelled with different pillar widths, rock mass classes and stress ratios in order to consider various site conditions, and the stabilities of the pillars were estimated by numerical analyses and scaled model tests. The strength-stress ratios of the pillar were obtained from three different methods which were using the stresses appeared at the middle point, the whole average and the left/right edges of the pillar. The strength-stress ratio of the pillar edges showed relatively conservative values among them, and it was also practically consistent with the tunnel excavating steps comprising the construction sequence analyses which included the partial excavation and the support system. Scaled model tests were also performed to investigate the tunnel stability, where it was found that cracks were progressively generated from the pillar edges toward the middle point of the pillar. Therefore, in order to both prevent the local damage of pillar and conservatively estimate the tunnel stability, it was thought to be an appropriate method using the strength-stress ratio obtained from the left/right edges of the pillar.

• Parasites, Hosts and Diseases
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• v.53 no.6
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• pp.719-724
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• 2015

Nematomorpha, horsehair or Gordian worms, include about 300 freshwater species in 22 genera (Gordiida) and 5 marine species in 1 marine genus (Nectonema). They are parasitic in arthropods during their juvenile stage. In the present study, the used gordian worm was found in the feces of a dog (5-month old, male) in July 2014. Following the worm analysis using light and scanning electron microscopes, the morphological classification was re-evaluated with molecular analysis. The worm was determined to be a male worm having a bi-lobed tail and had male gonads in cross sections. It was identified as Gordius sp. (Nematomorpha: Gordiidae) based on the characteristic morphologies of cross sections and areole on the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out, and the gordiid worm was assumed to be close to the genus Gordius based on a phylogenic tree analysis.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.2
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• pp.155-162
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• 2002

Cytochrome P45O (CYP) gene has been known to play one of the most important roles in metabolizing the exogenous materials. In insect, CYP is particularly known to detoxify toxic materials by adding oxygen molecule to the hydrophobic region of the materials. Thus, CYP-dependent metabolism is associated with the adaptation of insect to host plant chemicals. This in turn is known to be one of the driving forces for CYP diversification. In the present study, we cloned seven gene fragments of CYP 4 (CYP4) family from the midgut of the beet armyworm, Spodoptera exigua, through RT.PCT, Sequence analysis of the product showed the gene fragment to contain an open reading frame of ~150 amino acids, consisted of ~450 bp. The cloned gene fragments contained typical, conserved regions found in CYP4 family. Pairwise comparison of the deduced amino acid sequences among seven clones ranged in divergence from 0% to 52.86% and resulted in five distinct clones. The other two clones were identical or differ by one amino acid respectively to the corresponding clone, although each differed by ten nucleotides. Analysis of correlation between GenBank-registered, full length CYP4 and the cloned fragments resulted in statistically significant relationship ($r^{2}$ = 0.96085; p < 0.001), suggesting utility of the partial sequences as such full-length sequences. Phylogenetic analysis of the clones with GenBank-registered insect and mammal CYP4 family sequences by parsimony and several distance methods subdivided the clones into two groups: tones belonging to CYP4S and the others to CYP4M families.

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.19-24
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• 1996

To investigate the genes which is related to immune reaction of Bombyx mori, differential screening was carried out using naive and induced B. mori mRNA probe. To begin with, we constructed the cDNA library with mRNA isolated from fifth instar larvae injected with E. coli(4 X 106 cells/larva) using Uni ZAP XR vector kit. Thirty-two inducible cDNAs showing higher intensity on the induced mRNA probing membranes were selected. Partial nucleotide sequences of 29 clones were determined and their expessed sequence tags (ESTs) were produced. Nineteen ESTs in 29 ESTs were matched in GenBank database and the rest of them were found to be unknown. These unmatched ESTs were presumed to be novel genes. The nineteen ESTs contained variable genes related to biological process in Bombyx mori and four classes immune genes. Four clones, BmInc 6, 8, 18 and 27 were similar to two antibacterial peptide genes, hemolin gene and transferrin gene, respectively.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.12
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• pp.69-75
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• 2004

In the paper, system is combined multiband system with DS-UWB techniques with properties including low peak-to-average power ratio, robustness to multiuser interference and excellent security. Because each sub-band is not satisfied with coherence bandwidth, rake receiver in each sub-band is applied to the Proposed system receiver. Output of rake receiver is combined by using Maximal Ratio combining technology. In this paper we mathematically analyse the BER of the DS-UWB system with singleband and multiband systems in the narrow interference channel condition and multi user interference channel condition, the simulation results show that proposed scheme is getting robuster with increasing of the number of subbands.