The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Biological, Physical and Cytological Properties of Pepper mottle virus-SNU1 and Its RT-PCR Detection

  • Han, Jung-Heon (Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Choi, Hong-Soo (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Dong-Hwan (Department of Plant Science, Seoul National University) ;
  • Lee, Hung-Rul (Department of Plant Science, Seoul National University) ;
  • Kim, Byung-Dong (Center for Plant Molecular Genetics and Breeding Research)
  • Published : 2006.06.01
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Abstract

A strain of Pepper mottle virus (PepMov) was isolated from chili pepper plants in Korea. In host range study, this virus, designated PepMoV-SNU1, shared most characteristics with PepMoV isolates reported previously. Thermal inactivation point ($45^{\circ}C\;to\;75^{\circ}C$) and dilution end point ($10^{-1}\;to\;10^{-4}$) of PepMoV-SNU1 showed differences depending on the propagation hosts. Cylindrical and pinwheel-shaped inclusions were always observed in pepper leaf tissues infected with the virus alone. Unexpectedly, a special structure of pinwheel shaped inclusion surrounded with unknown small spots was also observed in the leaf section when co-infected with a strain of pepper mild mottle virus. The partial sequence of coat protein gene and 3' untranslated region of PepMoV-SNU1 showed 98% identity with those of other PepMoV isolates. A primer pair derived from 3' end of the coat protein gene and poly A tail regions were designed. Optimal detection condition of PepMoV-SNU1 by RT-PCR was tested to determine appropriate annealing temperature and additional volumes of oligo-dT (18-mer), dNTP, and Taq polymerase. Under the optimized condition, an expected 500 Up PCR-product was detected in pepper leaves infected with PepMoV-SNU1 but not in healthy plants.

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References

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