• 한국동물위생학회지
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• 제18권3호
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• pp.42-52
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• 1995

Anaplasmosis is most important tick-borne rickettsial diseases of domestic ruminant. with Anaptasma spp. as their respective causal agents. In order to the survey prevalence of anaplasmosis in Korean native goats, we examined the Giemsa's blood filrms from 552 grazing and 188 nongrazing-goats in Chonbuk area Namwon, Imsil and Sunchang. The conclusive diagnosis was made by observing the characteristic marginal or central bodies in the red blood cells. The infection rate of grazine and nongrazing-goats with Anaplasma spp. was 71.7% and 8.5%, respectively. In order to study about the therapeutic effects of drug for 40 grazing-oats infected with Anaptasma spp. in mountain-area, Berenil(diminazene acetate) was jntramuscularly injected (0.5㎖ /10kg B.W.). After 1 month, we don't detected the parasite in the blood films from 33 goats(82.5%), but no change of significant hematological values(PCV, ESR, WBC, RBC, MCH, MCHC, PLT, MPV, PDW and HB) was observed. These results indicated that the infection rate of Anaplasma spp. was higher on glazing than nongrazing-goats and Berenil as an antianaplasmal drug is recommended.

• Parasites, Hosts and Diseases
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• 제24권2호
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• pp.209-212
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• 1986

The present study was undertaken to evaluate the present status of intestinal parasitic infection in Seoul area, Korea. During the period from June 1985 to July 1986, a total of 5,251 stool samples were collected in Department of Clinical Pathology, Hanyang University Hospital and examined by formalin-ether sedimentation technique once for helminth ova and protozoan cysts. The results were summarized as follows: 1. The overall egg positive rate of intestinal helminthes was 2.53%; and 1.43% for Clonorchis sinensis, 0.7% for Trichuris trichiura, 0.13% for Metagonimus yokogawai, 0.06% for hookworm, and 0.02% for Ascaris lumbricoides, Diphyllobothrium latum and Hymenolepis nana, respectively. 2. The overall cyst positive rate of intestinal protozoa was 1.07%. Cyst positive rate was 0.06% for Entamoeba histolytica and 0.13% for Giardia lamblia, respectively. 3. Higher prevalence of intestinal parasitic infection was observed in male and in $21{\sim}50$ year-old groups. 4. The highest prevalence of Clonorchis sinensis infection was observed in 40th age group (3.4%), and male (2.3%) was more infected than female (0.7%) in general.

• 대한수의학회지
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• 제38권3호
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• pp.595-599
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• 1998

The present study was undertaken to develop the kit for indirect immunofluorescence antibody test(IFAT) and to investigate the prevalence of N caninum in cattle and dogs in Korea. The neo-antigen kit of IFAT developed in our laboratory was proved diagnostic efficacy by compared with the kit of veterinary medical research and development(VMRD). A survey of N caninum infections among cow and dogs in elevn areas of southern part of country was performed using a IFAT. The infection rate of 190 nationwide cattle was 8.4%(16/190) but was 75%(45/60) at P area in Chunnam province where the cattle showed the abortion repeatedly. Any of the dogs was not N caninum -positive in the Kangwon and Kyonggi areas.

• Parasites, Hosts and Diseases
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• 제58권1호
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• pp.1-5
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• 2020

Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis.

• Parasites, Hosts and Diseases
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• 제60권3호
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• pp.207-211
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• 2022

This study aimed to examine the distribution of gastrointestinal parasitic infections in domestic pigs in the Republic of Korea. From May 2020 to October 2021, 364 pig fecal samples were collected from 75 farms in 7 Provinces and microscopically examined. A total of 170 (46.7%) pigs were infected with at least one of the following parasites: Balantioides coli, strongyles, Ascaris suum, Trichuris suis, and coccidia. By parasite species, B. coli, strongyles, A. suum, T. suis, and coccidia oocysts or eggs were detected in 144 (39.6%), 24 (6.6%), 14 (3.8%), 4 (1.1%), and 1 (0.3%) samples, respectively. One hundred fifty-four, 15, and 1 cases showed single, double, and triple infections, respectively. Of the swine fecal samples from 75 farms, 69 specimens (92.0%) were infected with 1 or more parasites. All surveyed farms across the country exhibited a positive rate of over 30%, among which the highest positive rate was 65.0% in Chungcheongnamdo, and Jeollabuk-do was followed by 61.9%. Winter showed a statistically lower prevalence than other seasons. This study showed that gastrointestinal parasites are prevalent in pigs in Korea, although the diversity of parasites is low.

• Journal of Veterinary Science
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• 제24권1호
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• pp.8.1-8.12
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• 2023

Background: Intestinal parasites in livestock cause huge economic setbacks. Moreover, these parasites can threaten human health when also present in companion animals. Objectives: The study examined the prevalence and burden of intestinal parasites among roaming/migrating animals (goats, sheep, cattle, and dogs) to provide insights into the risk of potential human parasitic infections. Methods: A total of 1,741 fecal samples from goats (n = 920), sheep (n = 335), cattle (n = 230) and dogs (n = 256) were obtained randomly across 18 local government areas in Edo State, Nigeria. The parasite samples were recovered and identified under a microscope. Molecular tools were used to identify Toxocara spp. Results: Eighteen different parasites were isolated. Among the different groups of parasites observed, nematodes occurred the most, followed by protozoans. Among nematodes, Haemonchus was most prevalent in goats (28.04%) and sheep (29.85%), while Strongyloides (10.86%) and Bunostomum (8.69%) were relatively high in cattle. Strongyloides (3.9%), hookworm (3.9%), and Toxocara (3.12%) were the predominant parasites in dogs. For protozoan parasites, Eimeria was most common in all 4 animal hosts. Several goats (2.39%) and sheep (2.38%) tested positive for Fasciola spp. Molecular analysis confirmed Toxocara canis in dogs for the first time in Nigeria. Conclusions: The major parasites recovered from these roaming/migrating animals have zoonotic potentials that can threaten human health.

• Parasites, Hosts and Diseases
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• 제55권4호
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• pp.445-449
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• 2017

The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.

• 한국동물위생학회지
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• 제20권1호
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• pp.47-53
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• 1997

We have investigated the prevalence of canine heartworm (Dirofilaria immitis) infection among 100 mixed-breed dogs raised in Chonnam Province (Koheung, Posung, Changheung, and Hwasoon) and 100 dogs randomly selected from visiting dogs to the College of Veterinary Medicine, Chonnam National University for routine physical examination. Blood samples taken from dogs were examined for the presence of Dirofilaria immitis microfilaria by the modified Knott's test, and an antigen test which was based on the sandwich ELISA(DiroCHEK(equation omitted), Synbiotics, Inc., San Diego, California, USA) was used to detect the adult heartworm-specific antigen from serum samples. The results were then compared with those of the modified Knott's test. Five(2.5%) of the 200 examined dogs were microfilaria-positive, while 8 dogs(4%) were anti-gen-positive, which suggested that the antigen test was more sensitive than the microfilarial test in detecting heartworm infection. All dogs that were microfilaria-positive were also antigen-positive. The place of origin of the heartworm-positive dogs were of Naju (4/26, 15.4%), Koheung(3/32, 9.4%), and Hwasoon (1/19, 5.3%), while none of dogs raised in Kwangju, Posung, and Changheung areas were infected with the parasite. Although not significant, more infected dogs were observed in older ages, while the difference in infection rate between male (4/98, 4.1%) and female (4/102, 3.9%) was not recognized. Our results indicated that a relatively low infection rate was observed in dogs raised in Kwangju and Chonnam area compared to the infection rate reported previously in other areas of South Korea.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.745-747
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• 2015

Cryptosporidium, a protozoan parasite that causes watery diarrhea, is found worldwide and is common in areas with low water hygiene. In February 2014, 866 stool samples were collected from the inhabitants of 2 rural areas in White Nile State, Sudan. These stool samples were assessed by performing modified acid-fast staining, followed by examination under a light microscope. The overall positive rate of Cryptosporidium oocysts was 13.3%. Cryptosporidium oocysts were detected in 8.6% stool samples obtained from inhabitants living in the area having water purification systems and in 14.6% stool samples obtained from inhabitants living in the area not having water purification systems. No significant difference was observed in the prevalence of Cryptosporidium infection between men and women (14.7% and 14.1%, respectively). The positive rate of oocysts by age was the highest among inhabitants in their 60s (40.0%). These findings suggest that the use of water purification systems is important for preventing Cryptosporidium infection among inhabitants of these rural areas in Sudan.

• Parasites, Hosts and Diseases
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• 제57권2호
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• pp.153-159
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• 2019

Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.