Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2

  • Wanlop, Atcharaphan (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Wongsawad, Chalobol (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Prattapong, Pongphol (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Wongsawad, Pheravut (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Chontananarth, Thapana (Department of Biology, Faculty of Science, Srinakharinwirot University) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion)
  • Received : 2017.05.16
  • Accepted : 2017.06.21
  • Published : 2017.08.31
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Abstract

The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.

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References

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