• Archives of Plastic Surgery
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• v.48 no.3
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• pp.323-328
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• 2021

Background Lymphaticovenular anastomosis (LVA) is a minimally invasive surgical procedure used to treat lymphedema. Volumetric measurements and quality-of-life assessments are often performed to assess the effectiveness of LVA, but there is no method that provides information regarding postoperative morphological changes in lymphatic vessels and veins after LVA. Photoacoustic lymphangiography (PAL) is an optical imaging technique that visualizes the distribution of light-absorbing molecules, such as hemoglobin or indocyanine green (ICG), and provides three-dimensional images of superficial lymphatic vessels and the venous system simultaneously. In this study, we performed PAL in lymphedema patients before and after LVA and compared the images to evaluate the effect of LVA. Methods PAL was performed using the PAI-05 system in three patients (one man, two women) with lymphedema, including one primary case and two secondary cases, before LVA. ICG fluorescence lymphography was performed in all cases before PAL. Follow-up PAL was performed between 5 days and 5 months after LVA. Results PAL enabled the simultaneous visualization of clear lymphatic vessels that could not be accurately seen with ICG fluorescence lymphography and veins. We were also able to observe and analyze morphological changes such as the width and the number of lymphatic vessels and veins during the follow-up PAL after LVA. Conclusions By comparing preoperative and postoperative PAL images, it was possible to analyze the morphological changes in lymphatic vessels and veins that occurred after LVA. Our study suggests that PAL would be useful when assessing the effect of LVA surgery.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.151.1-151.1
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• 2014

Wake field effect on the electron beam from the undulator chamber in PAL-XFEL is analyzed. The wake field takeover some energy from the electron beam which will increase the energy spread of the electron beam. This will cause the degradation of the radiation power in PAL-XFEL. To decrease the effect, the surface of the undulator vacuum chamber should be fabricated with 200 nm surface roughness and 5 nm oxidation layer. In this presentation, the numerical calculation of the wake will be shown. Simulation results of the radiation generation in PAL-XFEL also will be presented.

• Journal of Life Science
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• v.19 no.9
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• pp.1226-1231
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• 2009

Enzyme substitution with recombinant phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is currently being explored for treatment of phenylketonuria (PKU), an autosomal recessive genetic disorder with mutations of the gene encoding phenylalanine-4-hydroxylase (EC 1.14.16.1). However, oral administration of PAL is limited because of proteolytic digestion in the gastrointestinal tract. The aim of this study was to determine the biochemical properties of PAL and delinate the susceptibility of wild-type PAL to pancreatic proteolysis by exploring several mutants, and to develop therapeutic drugs with PAL for PKU. The specific activity of PAL was assayed and its optimal pH, temperature stability, and intestinal protease susceptibility were investigated. Its $V_{max}$ values for phenylalanine and tyrosine were 1.77 and $0.47{\mu}mol$/ min/mg protein, respectively, and its $K_m$ values were $4.77{\times}10^{-4}$ and $4.37{\times}10^{-4}\;M$, respectively. PAL showed an optimal pH at 8.5, corresponding to the average pH range of the small intestine. It showed no loss of activity at $-80^{\circ}C$ for 5 months and possessed 93.4% of its activity under $4^{\circ}C$ for 4 wks. PAL was susceptible to chymotrypsin digestion and, to a lesser extent, to trypsin, elastase, carboxypeptidase A, and B. The trypsin and chymotrypsin cleaving sites were mutated to investigate protection from pancreatic digestion and the specific activities of these mutants were evaluated. The six mutants displayed low specific activities compared to the wild-type, suggesting that the primary trypsin and chymotrypsin cleaving sites may be essential for catalytic reaction. The PAL mutants could therefore be applied as a pretreatment modality without susceptibility to proteolytic attack, however, additional modification for enhancing enzymatic activity is needed to reduce the Phe levels effectively.

• Journal of the Korea Convergence Society
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• v.8 no.1
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• pp.35-42
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• 2017

Previously, we reported LVH peptiede derived from Aurelia aurita as cosmecetuticals with anti-winkle activity. In this study, we synthesized pal-LVH using palmitic acid to enhance skin permeability of LVH and examined the effects as cosmeceuticals of pal-LVH. To evaluate these effects, we performed cell toxicity, wound healing, and patch test for skin irritation with LVH and pal-LVH and compared these results for their effects. As a result. pal-LVH was not showed in cytotoxicity and allergenic effect like as LVH. Besides, pal-LVH had almost same excellent anti-ageing properties in high concentration and anti-winkle effect in low concentrationwas as LVH. These results suggested synthesis of palimitic acid and LVH did not affect any functions as cosmeceuticals with increasing skin permeability. Therefore, pal-LVH can be adaptable as new cosmecetuticals with anti-winkle and anti-ageing materials and applied in the development of medicine through various convergence study.

• Journal of Acupuncture Research
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• v.23 no.4
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• pp.49-60
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• 2006

Objectives : The aim of the study was to investigate the antihypertensive effect of Gyeok pal sang saeng Yeok chim Acupuncture in hypertensive patients. Methods : We measured the blood pressure of the patients who were admitted in the Oriental Medical Clinic of Brother from 13th February 2006 to 13th May 2006. We included the patients only in case of the systolic blood pressure was over 120mmHg or diastolic blood pressure was over 80mmHg, thirty patients were treated by Gyeok pal sang saeng Yeok chim Acupuncture. In order to evaluate the effect of the Gyeok pal sang saeng Yeok chim Acupuncture, the blood pressure and pulse rate were measured before and after Acupuncture procedure total 10 times. Results : There were significant decrease in the systolic blood pressure and significant decrease in the diastolic blood pressure treated by Gyeok pal sang saeng Yeok chim Acupuncture 10 times but pulse rate was not significantly decreased. The effect of Gyeok pal sang saeng Yeok chim Acupuncture by measurement time on blood pressure were follows: In a systolic blood pressure and diastolic blood pressure was gradually deceased significantly from 1st to 10th but pulse rate was not significantly decreased. Conclusion : These results suggest that Gyeok pal sang saeng Yeok chim Acupuncture is effective in decreasing the systolic and diastolic blood pressure.

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.256.2-256.2
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• 2008

• Preventive Nutrition and Food Science
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• v.22 no.3
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• pp.223-230
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• 2017

The purposes of this study were to produce polysaccharides from red pepper stems using different extraction methods and evaluate their chemical composition, in vitro biological capacities, and rheological properties. Two polysaccharides were extracted from red pepper stems using an autoclave and alkali treatments, and the extracts were named PAU and PAL, respectively. The contents of total phenolics and flavonoids were significantly higher in PAU than those in PAL. PAU exhibited greater scavenging activities on 2,2-diphenyl-1-picrylhydrazyl radicals, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radicals, superoxide radicals, and nitrite compared to PAL, suggesting that PAU served as better antioxidants. Similarly, in vitro inhibitory abilities against carbohydrate hydrolyzing enzymes of PAU were higher than those of PAL. Steady shear rheological analysis demonstrated that PAU showed higher psuedoplastic shear-thinning behavior compared to PAL. Based on the results from dynamic shear rheological properties, it was found that both samples had predominantly viscous behavior rather than elastic behavior.

• Proceedings of the KIEE Conference
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• 2000.07b
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• pp.1283-1285
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• 2000

고 주파수 스위칭 동작을 하는 DC/DC 컨버터 혹은 인버터를 효율적으로 제작하기 위하여 고주파용 펄스 변압기의 역할은 매우 중요하다. 동작주파수가 수십 혹은 수백 kHz일 경우에는 최소의 부피로 충분한 전력을 수용하면서 변압기를 설계 및 제작하기 위하여 코어 및 권선의 선정, 코어 및 권선의 손실, 변압기의 온도상승요소, 변압기의 발열, 변압기의 누설 인덕턴스, 권선 케페시턴스, 분포 케페시턴스 그리고 공진주파수 등을 고려하여 설계하고 시험 평가하여야 한다. 본 논문에서는 위의 여러가지 요소들을 고려하여 동작주파수가 100kHz인 DC/DC 컨버터 혹은 인버터를 고밀도 제작하기 위하여 고주파용 펄스변압기를 최소의 부피로 수kW 용량으로 변압기의 온도상승요소를 고려하여 설계, 제작 그리고 실험한 결과에 대하여 기술 하고자 한다.

• International Journal of Contents
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• v.15 no.3
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• pp.39-46
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• 2019

This study aimed to develop a peer-assisted learning (PAL) program for use in a core nursing skills education course and to investigate the effects of the education program on three key outcomes. A comparative study was conducted through the division of students of a core nursing skills education program into a treatment group and control group. After the programs, self-efficacy, confidence and satisfaction were compared between the groups. The comparison of these three factors indicated that the related scores were significantly higher in the treatment group receiving the education program with the PAL method than those of the control group receiving traditional education. We concluded that self-efficacy gave the nursing students the confidence that they could successfully perform their tasks and motivation to learn. Education programs using the PAL method are suggested as an effective method for the acquisition of skills among nursing students.

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.162-165
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• 2004

The authors' previous study found an interannual covariability between actual evapotranspiration (ET) and the Normalized Difference Vegetation Index (NDVI) over northern Asia. This result suggested that vegetation controls interannual variation in ET. In this prior study, NDVI data from the Pathfinder AVHRR Land (PAL) dataset were analyzed. However, studies of NDVI interannual change are subject to uncertainty, because NDVI data often contain errors associated with sensor- and atmosphere-related effects. This study is aimed toward reducing this uncertainty by employing NDVI dataset, from the Global Inventory Monitoring and Modeling Studies (GIMMS) group, in addition to PAL. The analysis was carried out for the northern Asia region from 1982 to 2000. 19-year interannual change in PAL-NDVI and GIMMS-NDVI were both compared with interannual change in model-assimilated ET. Although the correlation coefficient between GIMMS-NDVI and ET is slightly less than for PAL-NDVI and ET, for both NDVI datasets the annual maximum correlation with ET occurs in June, which is near the central period of the growing season. A significant positive correlation between GIMMS-NDVI and ET was observed over most of the vegetated land area in June as well as PAL-NDVI and ET. These results reinforce the authors' prior research that indicates the control of interannual change in ET is dominated by interannual change in vegetation activity.