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검색결과 330건 처리시간 0.027초

국내 개에서 발생한 아토피성 피부염에서 Dermatophagoides farinae의 항원 확인 (Identification of Allergens of Dermatophagoides farinae on Canine Atopic Dermatitis in Korea)

  • 조나영;박성준
    • 한국임상수의학회지
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    • 제31권6호
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    • pp.490-494
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    • 2014
  • 개의 아토피성 피부염에서 Dermatophagoides farinae의 항원을 immunoblot을 통해 확인하였다. 실험은 D. farinae에 의해 아토피성 피부염이 유발된 16마리와 임상적으로 건강한 10마리 개의 혈청으로 수행하였으며, SDS-PAGE를 실시하여 D. farinae 추출물의 항원들이 15에서 220 kDa 범위 내에 존재하는 것을 관찰하였다. Immunoblot 결과, 임상적으로 건강한 10마리 개의 혈청에서는 단백띠가 보이지 않았으며, D. farinae에 의해 아토피성 피부염이 유발된 16마리 개의 혈청을 이용한 실험에서는 14마리에서 109 kDa, 13마리에서 44 kDa, 11마리에서 177 kDa, 7마리에서 27 kDa, 5마리에서 75 kDa, 3마리에서 15 kDa의 단백띠가 관찰되었다. 즉, D. farinae에 의해 아토피성 피부염이 유발된 개체 중 50% 이상에서 관찰되는 주요 항원은 177 kDa, 109 kDa, 44 kDa 항원이었다. 본 실험과 같이 주요 항원들을 확인하는 이러한 연구는 아토피성 피부염에 대한 새로운 면역 치료의 전략과 제제의 개발에 기여할 수 있을 것으로 생각된다.

흰쥐 소뇌 연접후치밀질내 phosphotryrosine 함유 단백질에 대한 연구 (Studies on the phosphotyrsine-proteins in the rat cerbellar PSD fraction)

  • 전일수;함소희;고복현
    • 생명과학회지
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    • 제7권3호
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    • pp.198-204
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    • 1997
  • tyrosine 인산화효소계를 통한 신호전달은 신경의 발생과 연접환성조절에 중요한 역할을 한다. 흰쥐 소뇌의 연접후치밀질에 존재하는 tyrosine 함유 당백질들을 조사하기 위하여 immunoblot 분석을 한 결과, 소뇌 연접후치밀질의 전반적인 단백질조성은 전뇌와 비슷하였다. phosphotyrosine 특이성 항체로, immunobolot 한 결과 소뇌의 주된 tyrosine 인산화 단백질은 50 kD 크기의 새로운 단백질이었다. PSD-50로 명명한 이 단백질은 SDS-PAGE에서 $\alpha$CaMKII와 같은 위치에 이동하였다. 그러나 소뇌에는 전뇌에 비하여 적은량의 $\alpha$CaMKII가 존재함에도 불구하고 전뇌보다 더 강한 phosphotyrosine immunoblot signal을 보이는 것으로 보아 PSD-50는 아마도 $\alpha$CaMKII와는 다른 단백질로 추정된다.

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Spatial protein expression of Panax ginseng by in-depth proteomic analysis for ginsenoside biosynthesis and transportation

  • Li, Xiaoying;Cheng, Xianhui;Liao, Baosheng;Xu, Jiang;Han, Xu;Zhang, Jinbo;Lin, Zhiwei;Hu, Lianghai
    • Journal of Ginseng Research
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    • 제45권1호
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    • pp.58-65
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    • 2021
  • Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

EU REACH의 물질확인 방법론 고찰 (Perspective on substance identification in REACH)

  • 나진성;박광서;최은경;김상헌
    • 분석과학
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    • 제34권3호
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    • pp.99-114
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    • 2021
  • 물질확인은 화학물질 등록법에서 수행해야 하는 첫 단계로 올바른 물질확인은 이어지는 유해성 및 위해성 평가를 정확하게 효율적으로 하는데 필수적이다. 이에 톤수별 물질 등록을 모두 마친 EU REACH의 물질확인 절차를 유럽화학물질청 홈페이지 및 관련 지침서와 컨소시움 자료를 참고하여 살펴 보았고 물질확인과 물질의 동질성 확인 그리고 이를 위한 물질확인 프로파일 생성의 중요성을 고찰해보았다. 또한 기기분석에 의한 분석 결과 중 어떤 정보를 어떻게 정리하여 물질확인 프로파일을 생성하는지에 초점을 맞추어 몇 개 주요 유형별 물질을 사례로 제시해보았다. 분석과학은 화학물질 등록법이 정확하고 효율적으로 이행될 수 있도록 첫 단계인 물질확인에서 기여를 할 수 있는데 단, 법에 대한 이해를 기반으로 같은 물질의 등록자들의 분석 결과를 물질확인 프로파일로 최종 정리하여 공동 등록을 하는 등록자들과 관계 당국과 소통하는 것이 필수적이다.

Mannan-binding lectin of the sea cucumbers Stichopus japonicus has common antigenic determinants with human serum mannan-binding lectin

  • Bulgakov, A.A.;Petrova, I.Yu.;Vakhrusheva, N.M.;Eliseikina, M.G.
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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    • pp.530-530
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    • 2000
  • The host defense system or immune system of all modern animals has their roots in very ancient organisms. After analyzing literature data concerning properties of invertebrates and vertebrates lectins we suggest that mechanism of mannans recognition may exist in marine invertebrates, as a universal mechanism for homeostasis maintenance and host defense, and mannan-binding lectins family of vertebrates has ancient precursor, as was shown for another S-type lectins family. We carried out the screening of mannan-binding type lectin among different species of echinoderms inhabiting in Piter the Grate Bay, the sea of Japan. As a result, the C-type lectins (SJL-32) specific for high mannose glycans was isolated from the coelomic plasma of the sea cucumbers Stichopus japonicus by ion-exchange chromatography on a DEAE-Toyopearl 650M, affinity chromatography on a mannan-Sepharose 6B and gel filtration on a Sephacryl S-200. SJL-32 is homodimer with molecular mass about 32 kDa on SDS-PAGE under non-reducing conditions. Protein part of the lectin has high conteins Asn, Glu, Ser. Hemagglutination of trypsin-treated O blood group human erythrocytes by SJL-32 was competitively inhibited by high-branched -D-mannan composed of -1,2 and -1,6 linked D-mannopyranose residues. In contrast, a variety of mono-, oligo-, and polysaccharides composed of residues of galactose and fucose showed absence or little inhibitory activities. The lectin activity strong depends on Ca2+ concentration, temperature and pH. Monospecific polyclonal antibodies were obtained to the lectin. As was shown by ELISA assay, antibodies to SJL-32 cross-reacted with human serum mannan-binding lectin. This data allows making conclusion about common antigenic determinants and structural homology of both lectins. In our opinion, SJL-32 belongs to evolutionary high conservative mannan-binding lectins (MBLs) family and takes part in the host defense against pathogenic microorganisms.

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중국 인민일보를 통해서 보는 한국의 국가 이미지 (The image of South Korea reported by the People's Daily)

  • 우효영;김형곤
    • 디지털융복합연구
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    • 제20권3호
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    • pp.19-26
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    • 2022
  • 본 연구는 중국 <인민일보>의 한국 관련 기사에 대한 내용분석을 통해 한국의 국가 이미지가 어떻게 구축되고 있는가를 알아보는데 목적을 두고 있다. 연구대상은 2016년부터 2020년까지 <인민일보>에 게재된 한국 관련 기사 253건이다. 내용분석 방법을 이용해 기사 건수, 지면, 출처, 주제, 보도성향 등 5가지 항목에서 한국 관련 기사를 살펴보았다. 연구결과에 따르면, <인민일보>의 한국 관련 기사 건수는 2016년 이후 감소세를 보였고, 기사 주제는 정치, 사회 분야가 많았다. 2016년과 2017년에는 한국의 사드 배치 결정으로 인해 군사 분야 기사가 다수 게재되었고, 군사분야 기사에서만 부정적인 성향의 기사가 있었다. 대다수의 <인민일보> 한국 관련 기사는 한국을 긍정적으로 혹은 객관적으로 묘사하고 있음을 알 수 있었다. 이런 점으로 볼 때, <인민일보>는 긍정적인 한국의 국가 이미지를 구축하고 있음을 알 수 있다.

AR·QR 그림책 애니메이션의 시지각 확장성 연구: 그림책 <여기가 어디지?>의 설계적 특징을 중심으로 (A Study on Visual Perception Scalability of AR/QR Picture Book Animation: Focusing on the Design Characteristics of the Picture Book 〈Where is this?〉)

  • 박성원;박재윤
    • Journal of Information Technology Applications and Management
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    • 제30권1호
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    • pp.21-32
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    • 2023
  • This study presents a way to design picture books that allow children to experience indirectly through realistic media at home and educational institutions for children whose direct experiences and activities are reduced offline in the post-COVID-19 era. The development and popularization of realistic media such as AR continues to be grafted and developed in the picture book field, and the advantage of this picture book is that children at the stage before learning letters can feel the story and character situation of the book through the experience of AR and QR animation in the picture book. In this process, you can get access to imaginary areas that can not be experienced in flat-panel illustrations with spatial animation to help improve the scalability and literacy of the story contained on the page. Children's education through realistic animation can increase the learning effect and needs to continue after COVID-19. Therefore, by presenting the process of viewing and experiencing picture books using AR and QR, the result of the study suggests that the design process of realistic picture books is not just a combination of technology, but a process of expanding children's perception and imagination. This study aims to find out how visual perception is designed differently by analyzing the differences and characteristics of each animation design, focusing on the AR and QR picture book "Where am I?" published by the researcher, and to contribute to the active introduction and development of realistic animation in the field of children's picture books and education.

큰조롱과 넓은잎 큰조롱에서 신종 포티바이러스(큰조롱모자이크바이러스)의 동정 (Identification of a New Potyvirus, Keunjorong mosaic virus in Cynanchum wilfordii and C. auriculatum)

  • 이주희;박석진;남문;김민자;이재봉;손형락;최홍수;김정수;이준성;문제선;이수헌
    • 식물병연구
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    • 제16권3호
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    • pp.238-246
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    • 2010
  • 2006년 9월 충청북도 농업기술원 내 백미속 약용작물, 큰조롱과 넓은잎 큰조롱 시험포장에서 바이러스병 조사를 수행하였다. 각 시험구별 바이러스 발병률은 20-80%의 범위였으며, 뿌리로 번식한 경우가 종자로 번식한 경우보다 발병률이 두 배 정도로 높았다. 두 약용식물에서는 모자이크, 얼룩, 괴저, 황화, 퇴록반점, 기형 등 매우다양한 바이러스 병징이 관찰되었다. 전자현미경 분석결과 대부분의 시료에서 390-730 nm 길이의 사상형 입자가 관찰되었으며, 바이러스 병징을 보인 시료 중 일부에서는 바이러스 입자가 관찰되지 않은 것도 있었다. 전형적인 모자이크 증상을 보이는 큰조롱 잎을 순화하여 약 430-845 nm 길이의 사상형 입자를 얻을 수 있었다. 순화한 바이러스로부터 분리된 RNA를 이용하여 염기서열 분석을 실시한 결과 포티바이러스속 P3과 외피단백질 유전자의염기서열을 얻을 수 있었으며, BLAST 분석결과 포티바이러스속 바이러스들과 각각 약 26-38%와 62-72% 상동성을 나타내었다. 순화한 바이러스의 SDS-PAGE 분석에서 염기서열 분석으로 예상되는 약 30kDa의 외피단백질을 확인하였다. 7과 21종의 지표식물을 사용한 생물검정에서 Chenopodium quinoa에서 접종엽의 국부병반과 함께 전신적 황화반점 증상을 나타냈으며, 나머지 지표식물에서는 감염되지 않았다. 채집된 큰조롱과 넓은잎 큰조롱시료를 특이 프라이머를 이용한 RT-PCR 방법으로 진단한 결과 병징을 보인 모든 시료에서 양성반응이 나타났으며, 병징을 보이지 않는 7점 중 5점의 시료에서 양성반응을 나타내었다. 이러한 결과들을 종합해 볼 때 본 바이러스는 포티바이러스속의 새로운 종으로 확인되었으며, 우리나라가 원산지인 큰조롱에서 최초로 발견된 바이러스인 점에서 Keunjorong mosaic virus(KjMV)로 명명하고자 한다.

A Study on the Meaning and Strategy of Keyword Advertising Marketing

  • Park, Nam Goo
    • 유통과학연구
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    • 제8권3호
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    • pp.49-56
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    • 2010
  • At the initial stage of Internet advertising, banner advertising came into fashion. As the Internet developed into a central part of daily lives and the competition in the on-line advertising market was getting fierce, there was not enough space for banner advertising, which rushed to portal sites only. All these factors was responsible for an upsurge in advertising prices. Consequently, the high-cost and low-efficiency problems with banner advertising were raised, which led to an emergence of keyword advertising as a new type of Internet advertising to replace its predecessor. In the beginning of 2000s, when Internet advertising came to be activated, display advertisement including banner advertising dominated the Net. However, display advertising showed signs of gradual decline, and registered minus growth in the year 2009, whereas keyword advertising showed rapid growth and started to outdo display advertising as of the year 2005. Keyword advertising refers to the advertising technique that exposes relevant advertisements on the top of research sites when one searches for a keyword. Instead of exposing advertisements to unspecified individuals like banner advertising, keyword advertising, or targeted advertising technique, shows advertisements only when customers search for a desired keyword so that only highly prospective customers are given a chance to see them. In this context, it is also referred to as search advertising. It is regarded as more aggressive advertising with a high hit rate than previous advertising in that, instead of the seller discovering customers and running an advertisement for them like TV, radios or banner advertising, it exposes advertisements to visiting customers. Keyword advertising makes it possible for a company to seek publicity on line simply by making use of a single word and to achieve a maximum of efficiency at a minimum cost. The strong point of keyword advertising is that customers are allowed to directly contact the products in question through its more efficient advertising when compared to the advertisements of mass media such as TV and radio, etc. The weak point of keyword advertising is that a company should have its advertisement registered on each and every portal site and finds it hard to exercise substantial supervision over its advertisement, there being a possibility of its advertising expenses exceeding its profits. Keyword advertising severs as the most appropriate methods of advertising for the sales and publicity of small and medium enterprises which are in need of a maximum of advertising effect at a low advertising cost. At present, keyword advertising is divided into CPC advertising and CPM advertising. The former is known as the most efficient technique, which is also referred to as advertising based on the meter rate system; A company is supposed to pay for the number of clicks on a searched keyword which users have searched. This is representatively adopted by Overture, Google's Adwords, Naver's Clickchoice, and Daum's Clicks, etc. CPM advertising is dependent upon the flat rate payment system, making a company pay for its advertisement on the basis of the number of exposure, not on the basis of the number of clicks. This method fixes a price for advertisement on the basis of 1,000-time exposure, and is mainly adopted by Naver's Timechoice, Daum's Speciallink, and Nate's Speedup, etc, At present, the CPC method is most frequently adopted. The weak point of the CPC method is that advertising cost can rise through constant clicks from the same IP. If a company makes good use of strategies for maximizing the strong points of keyword advertising and complementing its weak points, it is highly likely to turn its visitors into prospective customers. Accordingly, an advertiser should make an analysis of customers' behavior and approach them in a variety of ways, trying hard to find out what they want. With this in mind, her or she has to put multiple keywords into use when running for ads. When he or she first runs an ad, he or she should first give priority to which keyword to select. The advertiser should consider how many individuals using a search engine will click the keyword in question and how much money he or she has to pay for the advertisement. As the popular keywords that the users of search engines are frequently using are expensive in terms of a unit cost per click, the advertisers without much money for advertising at the initial phrase should pay attention to detailed keywords suitable to their budget. Detailed keywords are also referred to as peripheral keywords or extension keywords, which can be called a combination of major keywords. Most keywords are in the form of texts. The biggest strong point of text-based advertising is that it looks like search results, causing little antipathy to it. But it fails to attract much attention because of the fact that most keyword advertising is in the form of texts. Image-embedded advertising is easy to notice due to images, but it is exposed on the lower part of a web page and regarded as an advertisement, which leads to a low click through rate. However, its strong point is that its prices are lower than those of text-based advertising. If a company owns a logo or a product that is easy enough for people to recognize, the company is well advised to make good use of image-embedded advertising so as to attract Internet users' attention. Advertisers should make an analysis of their logos and examine customers' responses based on the events of sites in question and the composition of products as a vehicle for monitoring their behavior in detail. Besides, keyword advertising allows them to analyze the advertising effects of exposed keywords through the analysis of logos. The logo analysis refers to a close analysis of the current situation of a site by making an analysis of information about visitors on the basis of the analysis of the number of visitors and page view, and that of cookie values. It is in the log files generated through each Web server that a user's IP, used pages, the time when he or she uses it, and cookie values are stored. The log files contain a huge amount of data. As it is almost impossible to make a direct analysis of these log files, one is supposed to make an analysis of them by using solutions for a log analysis. The generic information that can be extracted from tools for each logo analysis includes the number of viewing the total pages, the number of average page view per day, the number of basic page view, the number of page view per visit, the total number of hits, the number of average hits per day, the number of hits per visit, the number of visits, the number of average visits per day, the net number of visitors, average visitors per day, one-time visitors, visitors who have come more than twice, and average using hours, etc. These sites are deemed to be useful for utilizing data for the analysis of the situation and current status of rival companies as well as benchmarking. As keyword advertising exposes advertisements exclusively on search-result pages, competition among advertisers attempting to preoccupy popular keywords is very fierce. Some portal sites keep on giving priority to the existing advertisers, whereas others provide chances to purchase keywords in question to all the advertisers after the advertising contract is over. If an advertiser tries to rely on keywords sensitive to seasons and timeliness in case of sites providing priority to the established advertisers, he or she may as well make a purchase of a vacant place for advertising lest he or she should miss appropriate timing for advertising. However, Naver doesn't provide priority to the existing advertisers as far as all the keyword advertisements are concerned. In this case, one can preoccupy keywords if he or she enters into a contract after confirming the contract period for advertising. This study is designed to take a look at marketing for keyword advertising and to present effective strategies for keyword advertising marketing. At present, the Korean CPC advertising market is virtually monopolized by Overture. Its strong points are that Overture is based on the CPC charging model and that advertisements are registered on the top of the most representative portal sites in Korea. These advantages serve as the most appropriate medium for small and medium enterprises to use. However, the CPC method of Overture has its weak points, too. That is, the CPC method is not the only perfect advertising model among the search advertisements in the on-line market. So it is absolutely necessary that small and medium enterprises including independent shopping malls should complement the weaknesses of the CPC method and make good use of strategies for maximizing its strengths so as to increase their sales and to create a point of contact with customers.

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플래시메모리 SSD를 이용한 확장형 버퍼 관리 (Extended Buffer Management with Flash Memory SSDs)

  • 심도윤;박장우;김성탄;이상원;문봉기
    • 한국정보과학회논문지:데이타베이스
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    • 제37권6호
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    • pp.308-314
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    • 2010
  • 최근 들어, 플래시메모리의 가격이 지속적으로 낮춰지고, 플래시메모리 기반 SSD 컨트롤러 기술이 급격하게 발전하면서 중저가의 고성능 플래시 SSD가 시장에 널리 보급되고 있다. 하지만, 데이터베이스 분야에서 가격 동의 이유로 당분간 플래시 SSD가 하드디스크를 완전히 대체하기는 쉽지 않을 것이다. 대신 플래시 SSD의 빠른 성능을 캐시 용도로 활용하는 접근법이 현실적이고, 실제로 하드디스크와 플래시메모리를 하이브리드 형태로 사용하는 접근법들이 제시되었다. 본 논문에서는 기존의 접근법들과는 달리, 플래시 SSD를 데이터베이스의 버퍼에서 밀려나는 페이지들을 순차적으로 저장하고, 재 참조될 때 하드디스크 대신 플래시 SSD에서 읽혀지도록 하는 확장 버퍼 아키텍처를 제안한다. 플래시 SSD를 저장장치 레벨에서 캐시로 사용하는 기존 방법들에 비해, 플래시 SSD를 호스트 시스템에서 확장 버퍼로 사용함으로써 원기 측면에서 주 버퍼에서 밀려나는 웹 페이지(warm page)들에 대해 상당한 성능 개선을 이룰 수 있다. TPC-C 트레이스를 사용한 시뮬레이션 결과, 주 버퍼에 없는 페이지들이 확장 버퍼에서 찾아지는 적중률이 60%를 넘는 사실을 알 수 있었다. 이 확장 버퍼 아키텍처는, 동일한 비용을 지불하는 다른 접근법, 즉 DRAM을 버퍼로 추가하는 기법과 하드디스크를 추가하는 기법에 비해 가격 대비 성능 개선 효과가 높다.