• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.85-91
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• 2001

To construct an efficient Bacillus subtilis expression vector, strong promoters were isolated from the chromosomal DNA libraries of Clostridium acetobutylicum ATCC 4259, Thermoactinomyces sp. E79, and Bacillus thermoglucosidasius KCTC 3400. The $P_{C27}$ promoter cloned from the clostridial chromosmal DNA showed a 5-fold higher promoter strength than the $P_{SP02}$ promoter in the expression of the cat gene, and its sequence was estimated as an upstream region of the predicted hypothetical gene (tet-R family bacterial transcription regulator gene) in C. acetobutylicum. As a promoter element, $P_{C27}$ exhibited putative nucleotide sequences that can bind with bacterial RNAP and the 3'end of the 16S rRNA just upstream of the start codon. In addition, the promoter activity of $P_{C27}$ was distinctively repressed in the presence of glucose. Using $P_{C27}$ as the promoter element, a glucose controllable B. subtilis expression vector was constructed and the lipase gene from Staphylococcus haemolyticus KCTC 8957P was expressed in B. subtilis. When compared with the lipase expression by the T7 promoter induced by IPTG in E. coli, the $P_{C27}$ promoter showed about a 1.5-fold higher expression level in B. subtilis than that without induction.

• Journal of Ecology and Environment
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• 제38권3호
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• pp.383-388
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• 2015

Reservoirs consist of two different environments, the littoral and the pelagic zone, and different fishing gear is commonly used in each zone-gill nets in the pelagic zone and electrofishing in the littoral zone. However, an active fishing gear, the cast net, is normally used instead of electrofishing for scientific studies in South Korea. In order to estimate cast net effectiveness for determining fish status in reservoirs, the study was conducted at 15 reservoirs with two different fishing gears: a cast net in the littoral zone and gill nets in the pelagic zone. When combining catches of both gears, species richness increased substantially compared to using one gear only. There was a size difference in fish caught by each net, and small fish were predominantly caught with the cast net due to its small mesh size (7 mm). The combined length of six species, used for length-weight relationship analysis, collected with the cast net was smaller than that collected with gill nets (independent t-test, P < 0.05). In this study, cast net sampling provided sufficient data for the littoral zone, but not enough to identify the overall fish assemblage in studied reservoirs. Utilization of only one gear can therefore lead to substantial underestimation of fish status, and a combination of both gears is recommended for determining more reliable estimates of fish status in reservoirs.

• 한국임상수의학회지
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• 제37권5호
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• pp.249-254
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• 2020

Neutrophil extracellular trap (NET) formation is an immune response for the invasion of microbes. The purpose of this study is to examine the effect of zinc on NET formation of porcine peripheral blood polymorphonuclear cells (PMNs). The NET formation of PMNs was measured by fluorescence microplate reader. The production of tumor necrosis factor (TNF)-α in the culture supernatants from zinc-treated peripheral blood mononuclear cells (PBMCs) was measured by enzyme-linked immunosorbent assay (ELISA). Zinc itself did not have no effect on NET formation. However, the NET formation of PMNs was increased by culture supernatants from PBMCs treated with zinc. Also, the NET formation of PMNs was increased by recombinant porcine (rp) TNF-α. The production of TNF-α in PBMCs culture supernatants was shown to increase upon zinc treatments. These NET formations of PMNs increased by either culture supernatant from PBMCs treated with zinc or rpTNF-α were inhibited by treatment of anti-rpTNF-α polyclonal antibody (pAb). These results suggested that zinc has an immunostimulating effect on the NET formation of PMNs, which is mediated by TNF-α released from zinc-treated PBMCs. Therefore, zinc may play an important role for NET formation in the defense of porcine inflammatory diseases.

• 한국해양공학회지
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• 제23권4호
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• pp.25-31
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• 2009

To predict pollutants during the flood and dry season in Gwangyang Bay, the net-fluxes and pollutant material budgets of COD, T-N, and T-P were calculated in Gwangyang Bay using a 2-D hydrodynamic model. Calculating the net-flux for each area in Gwangyang Bay showed that the net-fluxes in regions IV, V, and VII were increasing, but those of regions II, III, and VI were decreasing. In budget calculations for COD, T-N, and T-P in Gwangyang Bay, it was estimated that during the dry season the COD is approximately 1.6 times higher than during the flood season. The T-N during the flood season is approximately 7 times higher than during the dry season. However, the material budget for T-P in Gwangyang Bay predicted that it is almost nonexistent. Moreover, the central part of Gwangyang Bay (Region IV) has the highest material budget of overall pollutants.

• 한국수산과학회지
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• 제42권1호
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• pp.83-88
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• 2009

The purpose of this paper is to obtain the fundamental data on the catch fluctuation in the pound set net according to the tide age from the catch recorded from the year 1997 to 2004 in the coastal waters of Guideok, Jeju. Total catch by the pound set net had a little connection with the tide age. During increasing tide, total catch were reduced slightly from the neap tide to the high tide while there seemed to be a little sign of rise in the total catch until decreasing tide. But in the relation between the catches and the tide age, the level of the correlation coefficient chosen at $p{\le}0.05$ was not significant. Therefore, the catch of the pound set net seemed not to be influenced by the tide age. In addition, CPUE at the high tide was higher than that at the neap tide. When the catch per operating frequency was graded in the order under 50 kg, 50-100 kg and 100-200 kg, the frequency rate by the pound set net was 38%, 19% and 19%, respectively.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권2호
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• pp.295-311
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• 2023

Cloud computing has evolved significantly, intending to provide users with fast, dependable, and low-cost services. With its development, malicious users have become increasingly capable of attacking both its internal and external security. To ensure the security of cloud services, encryption, authorization, firewalls, and intrusion detection systems have been employed. However, these single monitoring agents, are complex, time-consuming, and they do not detect ransomware and zero-day vulnerabilities on their own. An innovative Record and Replay-based hybrid Honeynet (R2NET) system has been developed to address this issue. Combining honeynet with Record and Replay (RR) technology, the system allows fine-grained analysis by delaying time-consuming analysis to the replay step. In addition, a machine learning algorithm is utilized to cluster the logs of attackers and store them in a database. So, the accessing time for analyzing the attack may be reduced which in turn increases the efficiency of the proposed framework. The R2NET framework is compared with existing methods such as EEHH net, HoneyDoc, Honeynet system, and AHDS. The proposed system achieves 7.60%, 9.78%%, 18.47%, and 31.52% more accuracy than EEHH net, HoneyDoc, Honeynet system, and AHDS methods.

• 한국수산과학회지
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• 제48권6호
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• pp.947-959
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• 2015

Variations in the species composition, biomass and size distribution of fisheries resources in the Uljin marine ranching area were investigated using trammel nets at two stations (artificial reef and natural rocky area) from 2009 to 2010. During the survey, a total of 74 species were sampled with a mean density of 132 ind./net and mean biomass of 21.56 kg/net. In the natural rocky area, a total of 45 species were sampled at a mean density of 202 ind./net and mean biomass of 28.81 kg/net, while in the artificial reef area, samples included a total of 56 species, with means of 62 ind./net and 14.30 kg/net. The dominant species, comprising over 3% of the total number of individuals, were Suberites ficus (30.8%), Ovalipes punctatus (19.2%), Paralichthys olivaceus (11.7%), Pleuronectes herzensteini (4.7%), Kareius bicoloratus (3.5%), Pseudopleuronectes yokohamae (3.5%) and Eopsetta grigorjewi (3.0%). The dominant species, in terms of biomass, comprising over 5% of the total biomass, were P. olivaceus (22.1%), S. ficus (18.7%), O. punctatus (7.2%), Hexagrammos otakii (6.6%), P. yokohamae (5.7%), K. bicoloratus and P. herzensteini (5.3%). A cluster analysis and non-metric multidimensional scaling (nMDS) analysis based on the Bray-Curtis similarity of fourth root transformed data for number of species and individuals, was divided into two groups: the artificial reef area (group A) and the natural rocky area (group B).

• 대한의용생체공학회:의공학회지
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• 제43권5호
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• pp.331-340
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• 2022

Early afterdepolarization (EAD), a significant cause of fatal ventricular arrhythmias including Torsade de Pointes (TdP) in long QT syndromes, is a depolarizing afterpotential at the plateau or repolarization phase in action potential (AP) profile early before completing one pace. AP duration prolongation is related to EAD but is not necessarily accounted for EAD. Several computational studies suggested EAD can form from an abnormality in the late plateau and/or repolarization phase of AP shape. In this sense, we hypothesized the slope during repolarization has the characteristics to predict TdP risk, mainly focusing on the maximum slope during repolarization (dVm/dt_{max_repol}). This study aimed to predict the sensitivity of dVm/dt_{max_repol} to ion channel conductances as a TdP risk metric through a population simulation considering multiple effects of simultaneous reduction in six ion channel conductances of g_NaL, g_Kr, g_Ks, g_to, g_K1, and g_CaL. Additionally, we verified the availability of dVm/dt_{max_repol} for TdP risk prediction through the correlation analysis with qNet, the representative TdP metric. We performed the population simulations based on the methodology of Gemmel et al. using the human ventricular myocyte model of Dutta et al. Among the sixion channel conductances, dVm/dt_{max_repol} and qNet responded most sensitively to the change in g_Kr, followed by g_NaL. Furthermore, dVm/dt_{max_repol} showed a statistically significant high negative correlation with qNet. The dVm/dt_{max_repol} values were significantly different according to three TdP risk levels of high, intermediate, and low by qNet (p<0.001). In conclusion, we suggested dVm/dt_{max_repol} as a new biomarker metric for TdP risk assessment.

• Asian-Australasian Journal of Animal Sciences
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• 제26권9호
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• pp.1282-1288
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• 2013

A comparative slaughter trial was conducted to estimate the phosphorus (P) requirement for maintenance and growth of crossbred lambs of Dorper with a Chinese indigenous sheep breed, thin-tailed Han sheep. Thirty-five Dorper${\times}$thin-tailed Han crossbred, noncastrated ram lambs ($20.3{\pm}0.22kg$ of shrunk body weight (SBW)) were used. Seven lambs were randomly chosen and slaughtered at 20 kg SBW as the baseline group for measuring initial body composition. Another seven lambs were also randomly chosen and offered a pelleted mixed diet for ad libitum intake and slaughtered at 28 kg SBW. The remaining 21 sheep were randomly divided into 3 groups with 7 sheep each and subject to the same diet of either 70 or 40% of ad libitum intake. The 3 groups were slaughtered when the sheep fed ad libitum reached 35 kg of SBW. Body P contents were determined after slaughter. The results showed that the net P requirement for maintenance was 30.0 mg/kg of empty body weight (EBW) or 23.4 mg/kg body weight (BW), and the P requirement for growth decreased from 5.3 to 5.0 g/kg of EBW gain as the lamb grew from 20 to 35 kg. The net P requirement for growth of Dorper${\times}$thin-tailed Han crossbred ram lambs was lower than that of sheep adopted by the American nutritional system.

• 소성∙가공
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• 제10권6호
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• pp.500-508
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• 2001

The integration of Rapid prototyping (RP) and Rapid Tooling (RT) has the potential for rapid net shaping of thee-dimensional parts, which have a geometrical complexity. In this study, a new R)P process, (VLM-SP), was proposed to manufacture net shapes of three-dimensional prototypes and it was shown that VLM-SP is an effective and economic process through the comparison of building time, building cost and dimensional accuracy for the test parts with the commercial RP processes ; LOM and FDM. In addition, the metal parts, which are a spanner shape and a clover punch, were produced by the plaster casting as one of RT using the prototypes of VLM-SP.