• Title/Summary/Keyword: pH studies

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EXPERIMENTAL STUDIES OF THE EFFECT OF EROSION ON THE TOOTH ENAMEL SURFACE BY ACID BEVERAGES PURCHASED ON THE RETAIL MARKET (수종의 시판 청량음료가 치아표면 법랑질 침식에 미치는 영향에 관한 실험적 연구)

  • Ha, Joo-Sik;Kim, Chong-Youl
    • The Journal of the Korean dental association
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    • v.19 no.11 s.150
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    • pp.949-956
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    • 1981
  • The author collected some acid containing beverages to observe the influence which acid containing beverages affect the tooth surface tissue and the resistance of tooth which was treated with fluoride and counterpart tooth in the acid containing beverages and comparatively observing the erosion of enamel surface, the author came to the following conclusion. 1. Acid containing beverages on the market eroded the tooth surface tissue and the erosion degree was determined by the pH concentration of beverages. 2. the erosion aspect of the tooth surface in terms of acid containing beverages indicated some on the surface and surrounding enamel rod in the beverages which the pH was comparatively high, and the erosion was observed greatly in all enamel including the enamel rod in the beverages which pH was high. 4. When one drinks beverages, if beverges' pH is high, there are no serious harmful, but the author recommended not drinking the beverages which pH is low under the situation lack of the proper preventive dentistry technique.

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Studies on the Conditions of Enzyme Production of Endocellular Cytosine Deaminase from Aspergillus fumigatus IFO 5840 (Aspergillus fumigatus IFO 5840의 균체내 Cytosine Deaminase의 생성에 관한 연구)

  • 김재근;하영득
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.2
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    • pp.179-186
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    • 1991
  • The nutritional requirement and cultural condition such as carbon and nitrogen sources, cultural temperature, initial pH, cultural time and aeration for the production of endocellular cytosine deaminase from Aspergillus fumigatus IFO 5840 were investigated. The cultural broth giving maximum cytosine deaminase yield was found to consist of 2% glucose as a carbon source and 1% yeast extract and 0.1% peptone as a nitrogen source. Optimal initial pH of the culture broth was pH 8.5 and the enzyme production in the cell usually reached a maximum after 28 hours of cultivation in the 500ml shaking flask containing 100ml broth at $30^{\circ}C$. The endoenzyme production of the used strain was inhibited by inorganic nitrogen, but activated by orgainc nitrogen, yeast extract.

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Helicobacter pylori Infection and a P53 Codon 72 Single Nucleotide Polymorphism: a Reason for an Unexplained Asian Enigma

  • Pandey, Renu;Misra, Vatsala;Misra, Sri Prakash;Dwivedi, Manisha;Misra, Alok
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.21
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    • pp.9171-9176
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    • 2014
  • Aim: P53, the most commonly mutated tumor suppressor gene in all types of human cancer, is involved in cell cycle arrest and control of apoptosis. Although p53 contains several polymorphic sites, the codon 72 polymorphism is by far more common. There are divergent reports but many studies suggest p53 pro/pro SNP may be associated with susceptibility to developing various cancers in different regions of the world. The present study aimed to find any correlation between H. pylori infection and progression of carcinogenesis, by studying apoptosis and the p53 gene in gastric biopsies from north Indian population. Materials and Methods: A total of 921 biopsies were collected and tested for prevalence of H. pylori by rapid urease test (RUT), imprint cytology and histology. Apoptosis was studied by the TUNEL method. Analysis of p53 gene polymorphism at codon 72 was accomplished by PCR using restriction enzyme BstU1. Observation: Out of 921 samples tested 56.7% (543) were H. pylori positive by the three techniques. The mean apoptotic index (AI) in the normal group was 2.12, while gastritis had the maximum 4.24 followed by gastric ulcer 2.28, gastropathy 2.22 and duodenal ulcer 2.08. Mean AI in cases with gastric cancer (1.72) was less than the normal group. The analysis of p53 72 SNP revealed that p53 (Arg/Arg), (Pro /Arg) variant are higher (40.59% & 33.66%) as compared to p53 pro/pro variant (25.74%) inthe healthy population. Conclusions: The North Indian population harbors Arg or Pro/Arg SNP that is capable of withstanding stress conditions; this may be the reason of low incidence of gastric disease in spite of high infection with H. pylori. There was no significant association with H. pylori infection and AI. However, there is increased apoptosis in gastritis which may occur independent of H. pylori or p53 polymorphism.

Studies on Frozen Storage for Korean Traditional Poultry (한국산(韓國産) 전통(傳統) 가금육(家禽肉)의 냉동저장(冷凍貯藏)에 관(關)한 연구(硏究))

  • Park, Young Deok;Chang, Kyu Seob;Kang, Hyun Ah
    • Korean Journal of Agricultural Science
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    • v.20 no.2
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    • pp.181-188
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    • 1993
  • Frozen korean traditional poultries(Manyker, Korean native fowl, Ogol fowl and Pheasant) were thawed at $5^{\circ}C$, and $20^{\circ}C$ in the air, and using a microwave oven. Effect of thawing methods on the quality of Korean poultry was investigated. The pH of the poultry muscle was lowered after thawing in all treatments. The pH of the muscle thawed by microwave oven was the lowest. The quantity of drip released after thawing was the least in a microwave thawing. The quantity of drip in Maniker and Pheasant was loss than Ogol fowl. The relationship between pH and water binding capacity showed highly significant, and the drip quantity after thawing increased as the pH decreased. Leg muscle has higher strength than breast muscle in all the Korean poultry. Shear strength of all the poultry muscle showed closely relative related to the pH. As the pH lowered, the shear strength of the muscle was also decreased.

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Studies on Solvent Extraction Using Salphen for Separative Determination of Trace Fe(II) and Fe(III) in Water Samples

  • Kim, Eun-Jook;Kim, Young-Sang;Choi, Jong-Moon
    • Bulletin of the Korean Chemical Society
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    • v.29 no.1
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    • pp.99-103
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    • 2008
  • Solvent extraction using salphen as a ligand has been investigated for the selective separation and determination of trace Fe(II) and Fe(III). A salphen ligand was synthesized, and solvent extraction variables, such as solution pH, the concentration of salphen, the type of organic solvent, auxiliary agents, oxidants and the effect of interference were optimized. Salphen is stable at pH 3-4, and Fe(III)-salphen complexes can be selectively extracted into an MIBK(4-methyl-2-pentanone) phase from an aqueous solution within this pH range. For the determination of the total amount of iron in 100 mL of aqueous solution, Fe(II) ions were completely oxidized using 0.05 mL of 3.5% H2O2 without side reactions. To evaluate its applicability, the proposed method was applied to determine trace Fe(II) and Fe(III) in several kinds of water samples. Reproducible results were obtained with RSD of less than 3.0%, and the recoveries for this reliability were obtained with 91-112%.

ANION INDUCED BLUE TO PURPLE TRANSITION IN BACTERIORHODOPSIN

  • Singh, Anil K.;Kapil, Mrunalini M.
    • Journal of Photoscience
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    • v.3 no.2
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    • pp.71-76
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    • 1996
  • Anil K. Singh, Mrunalini M. Kapil, Department of Chemistry, Indian Institute of Technology Bombay - 400076, INDIA Purple membrane (PM, $\lambda$$_{max}$ 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue ($\lambda$$_{max}$ 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI$^-$ and HPO$_4^{2-}$. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI$^-$ and HPO$_4^{2-}$ show marked pH effect. At pH 1.0 the efficiency of regeneration of the purple chromophore is greater than at pH 2.0, for the same anion concentration. Fluorescence and circular dichroic studies indicate that the proteins do not undergo drastic changes at the secondary' or tertiary structure level and the native structure is preserved during this transition. However, chromophoric-site interactions between retinal and the apoprotein are affected during this colour transition. A molecular mechanism is advanced for this transition.

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Studies on the Optimum Surface Treatment Conditions and the Interfacial Bond Strength of Glass fiber/Nylon 6 Composites (유리섬유/Nylon 6 복합재료의 표면처리 최적조건과 개별결합력에 관한 연구)

  • 나성기;박종신
    • Proceedings of the Korean Fiber Society Conference
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    • 1997.04a
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    • pp.26-31
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    • 1997
  • 유리섬유/nylon 6 복합재료의 계면결합강도를 증가시키기 위하여 r-APS(r-Aminopropyltriethoxysilane)로 유리섬유의 표면을 처리 하였다. 이때 표면처리의 최적 조건을 찾기위해서 처리후 기기분석과 계면결합강도 측정 등을 하였다. 농도, pH, 처리시간, 온도를 변화시키면서 표면처리를 한 후 흡착량을 살펴본 결과 처리 농도에 의해서는 흡착량이 단조증가하였으며 처리시간에 따라서는 5분정도에서, 처리온도에 의해서는 30C 부근에서 최대 흡착량을 보였다. 또한 pH에 따른 흡착량은 silane의 고유 pH인 10.5부근에서 최대치를 나타냈다. FR-IR 분석에 의하면 NH2의 NH3 bending mode가 1607cm-1, 1575cm-1에서 나타났으며 SiOH의 SiO band는 960cm-1에서 나타났다. XPS를 통해서는 N ls와 Si 2p의 존재를 확인할 수 있었다. 표면처리된 유리섬유와 matrix인 nylon 6를 이용해 단섬유내장시편을 만들어 fragmentation test를 한 결과 계면결합강도는 약 5분의 처리시간과 1%(wt%)의 농도에서 최대값을 보였다.

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Volatiles from the Maillard Reaction of L-Ascorbic Acid and L-Alanine at Different pHs

  • Yu, Ai-Nong;Deng, Qi-Hui
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1495-1499
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    • 2009
  • The volatiles formed from the reactions of L-ascorbic acid with L-alanine at 5 different pH (5, 6, 7, 8, or 9) and $140{\pm}2^{\circ}C$ for 2 hr was performed using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis were identified to be 25 different kinds. The reaction between L-ascorbic acid and L-alanine led mainly to the formation of pyrazines. Many of these were alkylpyrazines, such as 3-ethyl-2,5-dimethylpyrazine, 2,5-dimethylpyrazine, 2-ethyl-5-methylpyrazine, 3,5-diethyl-2-methylpyrazine, methylpyrazine, 2-ethyl-6-methylpyrazine, and 2,3-diethyl-5-methylpyrazine, other compounds identified were furans, phenols, benzoquinones, 2,4,6-trimethylpyridine, and 2-methylbenzoxazole. The studies showed that furans, such as furfural and benzofuran were formed mainly at acidic pH. In contrast, higher pH values could promote the production of pyrazines.

Studies on the pretense produced by Penicillium species (Penicillium속(屬)이 생산(生産)하는 alkaline pretease에 관(關)하여)

  • Kim, Kyung-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.6 no.1
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    • pp.27-33
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    • 1977
  • The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7-saturated ammonium sulfate, then dialysis for 3days. The activity of this enzyme was determined by Folin's colorimetric method. The results were as follows; 1. The optimum pH and temperature of this enzyme were pH 8.4 and $45^{\circ}C$. 2. This enzyme was stable at pH $7.0{\sim}9.0$. 3. This enzyme was not inactivated by treatment in lower temperature than $30^{\circ}C$. 4. The activity of this enzyme was strongly inhibited by $Hg^{++}$ and $Cu^{++}$, but slightly by $Ag^+$ 5. This enzyme was not inhibited by cystein, thiourea, ${\varepsilon}-aminocaproic$ acid, 2, 4-DNP, EDTA but strongly inhibited by PCMB.

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Isothermal and Kinetic Studies of the Adsorption Removal of Pb(II), Cu(II), and Ni(II) Ions from Aqueous Solutions using Modified Chara Sp. Algae

  • Kalash, Khairi R.;Alalwan, Hayder A.;Al-Furaiji, Mustafa H.;Alminshid, Alaa. H.;Waisi, Basma I.
    • Korean Chemical Engineering Research
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    • v.58 no.2
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    • pp.301-306
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    • 2020
  • We investigated the individual biosorption removal of lead, copper, and nickel ions from aqueous solutions using Chara sp. algae powder in a batch mode. The impact of several parameters, such as initial concentration of the metal ions, contacting time, sorbent dose, and pH on the removal efficiency, was investigated. The maximum removal efficiency at optimum conditions was found to be 98% for Pb(II) at pH = 4, 90% for Cu(II) at pH = 5, and 80% for Ni(II) at pH = 5. The isotherm study was done under the optimum conditions for each metal by applying the experimental results onto the well-known Freundlich and Langmuir models. The results show that the Langmuir is better in describing the isotherm adsorption of Pb(II) and Ni(II), while the Freundlich is a better fit in the case of Cu(II). Similarly, a kinetic study was performed by using the pseudo-first and second-order equations. Our results show that the pseudo-second-order is better in representing the kinetic adsorption of the three metal ions.