• 제목/요약/키워드: pH sensing

검색결과 251건 처리시간 0.031초

무궁화꽃을 이용한 L-Arginine Sensor 開發에 關한 硏究 (The Development of Arginine-Selective Membrane Electrode Using Tissue Slices of the Rose of Sharon)

  • 김의락;남기재;최성문
    • 대한화학회지
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    • 제36권5호
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    • pp.753-759
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    • 1992
  • 흰 무궁화꽃의 씨방을 이용하여 Tissue electrode를 제조하고, L-arginine을 정량하기 위한 최적조건을 조사한 결과 0.1M phosphate 완충용액에서 pH 8.0, 온도 $25^{\circ}C$, 조직두께 $20 {\mu}m$이었을 때이며, 이에 정량 가능한 직선범위는 $5.0 {\times} 10^{-3}M $$1.0 {\times} 10^{-1}M$이었으며, 감응도는 54.0 mV/decade로 나타났고, 감응시간은 7∼8분이 소요되었다. $K_m$값은 $6.4 {\times} 10^{-6}(M)$이다.

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14N Mines Pulsed-ENDOR of Proximal Histidine and Heme of Aquometmyoglobin and Fluormetmyoglobin

  • Lee, Hong-In
    • Bulletin of the Korean Chemical Society
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    • 제23권12호
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    • pp.1769-1772
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    • 2002
  • Previous $^{19}F\;and\;^{1,2}H$ electron-nuclear double resonance (ENDOR) study of fluorometmyoglobin (MbF) in frozen-solution state provided sensitive tools sensing subtle structural changes of the heme that are not obtainable from X-ray. [Fann et al., J. Am. Chem. Soc. 1995, 117, 6019] Because of the intrinsic inhomogeneouse EPR line broadening effect of MbF in frozen-solution state, detection of the intrinsic inhomogeneouse EPR line broadening effect of MbF in frozen-solution state, detection of the electronic and geometrical changes of the heme ring itself and the proximal histidine by using $^{14}N$ CW ENDOR was interfered. In the present study, hyperfine-sensitive $^{14}N$ Mims ENDOR technique of pulsed-EPR was employed to probe the changes. With two different $\tau$ values of 128 and 196 ns, $^{14}N$ ENDOR signals of the heme and proximal histidine were completely resolved at $g'_{II}(=g_e=2)$. This study present that X-band $^{14}N$ Mims ENDOR sequence can sensitively detect the small changes of the spin densities and p orbital populations of the proximal and the heme nitrogens, caused by ligand and pH variation of the distal site.

Simultaneous Electroanalysis of Nitric Oxide and Nitrite

  • Oritani, Tadato;Okajima, Takeyoshi;Kitamura, Fusao;Ohsaka, Takeo
    • 전기화학회지
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    • 제5권4호
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    • pp.209-211
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    • 2002
  • The simultaneous sensing of nitric oxide (NO) and its metabolite, nitrite $(NO_2^-)$ has been studied by Osteryoung square-wave voltarnmetery (OSWV) in physiological pH solution (0.1 M phosphate buffer solution, pH 7.2). Using an electrochemically pretreated glassy carbon (GC) electrode, OSWV was successfully applied to observe the well-separated oxidation peaks at ca. 0.58 and 0.80V vs. Ag/AgCI for NO and $(NO_2^-)$, respectively. This clear separation between the NO and $(NO_2^-)$ oxidation peaks may be due to the formation of surface oxides (e.g., quinone (C=O) or carboxylic $(COO^-)$ group) and surface defects introduced by the electrochemical pretreatment of GC electrodes.

Decentralized civil structural control using real-time wireless sensing and embedded computing

  • Wang, Yang;Swartz, R. Andrew;Lynch, Jerome P.;Law, Kincho H.;Lu, Kung-Chun;Loh, Chin-Hsiung
    • Smart Structures and Systems
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    • 제3권3호
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    • pp.321-340
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    • 2007
  • Structural control technologies have attracted great interest from the earthquake engineering community over the last few decades as an effective method of reducing undesired structural responses. Traditional structural control systems employ large quantities of cables to connect structural sensors, actuators, and controllers into one integrated system. To reduce the high-costs associated with labor-intensive installations, wireless communication can serve as an alternative real-time communication link between the nodes of a control system. A prototype wireless structural sensing and control system has been physically implemented and its performance verified in large-scale shake table tests. This paper introduces the design of this prototype system and investigates the feasibility of employing decentralized and partially decentralized control strategies to mitigate the challenge of communication latencies associated with wireless sensor networks. Closed-loop feedback control algorithms are embedded within the wireless sensor prototypes allowing them to serve as controllers in the control system. To validate the embedment of control algorithms, a 3-story half-scale steel structure is employed with magnetorheological (MR) dampers installed on each floor. Both numerical simulation and experimental results show that decentralized control solutions can be very effective in attaining the optimal performance of the wireless control system.

KOREA-AUSTRIA-AN EXAMPLE OF A CO-OPERATION BETWEEN DATA PROVIDER AND DATA USER ACROSS THE CONTINENTS

  • Kressler, F.P.;Kim, Y.S.;Triebnig, G.;Hoffmann, C.;Schiller, C.
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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    • pp.798-802
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    • 2002
  • Data provider and data user are linked together by suitable data. This implies that for a successful relationship the provider knows what kind of data, whether original or processed, is needed by the user and that the user articulates his needs to the provider. The co-operation between Korea and Austria aims at identifying these needs and to provide suitable routines for the processing of satellite data. Partners of this co-operation are the Korea Aerospace Research Institute, ARC Seibersdorf research and GeoVille. While the focus of the former lies in the development and running of satellite systems, the latter two have experience in the development and set up of applications in the field of remote sensing. Based on the complementary know-how by the institutions involved, projects were set up in Korea and Austria to fulfill this need and establish a pilot data feed for governmental applications.

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H2S Gas Sensing Properties of SnO2:CuO Thin Film Sensors Prepared by E-beam Evaporation

  • Sohn, Jae-Cheon;Kim, Sung-Eun;Kim, Zee-Won;Yu, Yun-Sik
    • Transactions on Electrical and Electronic Materials
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    • 제10권4호
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    • pp.135-139
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    • 2009
  • $H_2S$ micro-gas sensors have been developed employing $SnO_2$:CuO composite thin films. The films were prepared by e-beam evaporation of Sn and Cu metals on silicon substrates, followed by oxidation at high temperatures. Results of various studies, such as scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) reveal that $SnO_2$ and CuO are mutually non-reactive. The CuO grains, which in turn reside in the inter-granular regions of $SnO_2$, inhibit grain growth of $SnO_2$ as well as forming a network of p-n junctions. The film showed more than a 90% relative resistance change when exposed to $H_2S$ gas at 1 ppm in air at an operating temperature of $350^{\circ}C$ and had a short response time of 8 sec.

Application of Hydrogenated Amorphous Silicon(a-Si : H) Radiation Detectors in Nuclear Medicine

  • Lee, Hyoung-Koo;Mendez, Victor-Perez;Shinn, Kyung-Sub
    • 한국의학물리학회지:의학물리
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    • 제6권1호
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    • pp.65-77
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    • 1995
  • A new gamma camera using a-Si : H photodetectors has been designed for the imaging of heart and other small organs. In this new design the photomultiplier tubes and the position sensing circuitry are replaced by 2-D array of a-Si : H p-i-n pixel photode tectors and readout circuitry which are built on a substrate. Without the photomultiplier tubes this camera is light weight, hence can be made portable. To predict the characteristics and the performance of this new gamma camera we did Monte Carlo simulations. In the simulations 128${\times}$128 imaging array of various pixel sixes were used. $\^$99m/Tc(140keV)and $\^$201/Tl(70keV) were used as radiation sources. From the simulations we could obtain the resolution of the camera and ther overall system, and the blurring effects due to scattering in the phantom. Using the Wiener filter for image processing, restoration of the blurred image could be achieved. Simulation results of a-Si : H based gamma camera were compared with those of a conwentional gamma camera.

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산화주석을 기반으로 한 DMMP 후막가스센서 제작 (fabrication of DMMP Thick Film Gas Sensor Based on SnO2)

  • 최낙진;반태현;곽준혁;백원우;김재창;허증수;이덕동
    • 한국전기전자재료학회논문지
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    • 제16권12S호
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    • pp.1217-1223
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    • 2003
  • Nerve gas sensor based on tin oxide was fabricated and its characteristics were examined. Target gas is dimethyl methyl phosphonate(C$_3$ $H_{9}$ $O_3$P, DMMP) that is simulant gas of nerve gas. Sensing materials were Sn $O_2$ added a-Al$_2$ $O_3$ with 0∼20wt.% and were physically mixed each material. They were deposited by screen printing method on alumina substrate. The sensor device was consisted of sensing electrode with interdigit(IDT) type in front and a heater in back side. Total size of device was 7${\times}$10${\times}$0.6㎣. Crystallite size & phase identification and morphology of fabricated Sn $O_2$ powders were analyzed by X-ray diffraction and by a scanning electron microscope, respectively. Fabricated sensor was measured as flow type and resistance change of sensing material was monitored as real time using LabVIEW program. The best sensitivity was 75% at adding 4wt.% $\alpha$-Al$_2$ $O_3$, operating temperature 30$0^{\circ}C$ to DMMP 0.5ppm. Response and recovery time were about 1 and 3min., respectively. Repetition measurement was very good with $\pm$3% in full scale.TEX>$\pm$3% in full scale.

Design of wireless sensor network and its application for structural health monitoring of cable-stayed bridge

  • Lin, H.R.;Chen, C.S.;Chen, P.Y.;Tsai, F.J.;Huang, J.D.;Li, J.F.;Lin, C.T.;Wu, W.J.
    • Smart Structures and Systems
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    • 제6권8호
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    • pp.939-951
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    • 2010
  • A low-cost wireless sensor network (WSN) solution with highly expandable super and simple nodes was developed. The super node was designed as a sensing unit as well as a receiving terminal with low energy consumption. The simple node was designed to serve as a cheaper alternative for large-scale deployment. A 12-bit ADC inputs and DAC outputs were reserved for sensor boards to ease the sensing integration. Vibration and thermal field tests of the Chi-Lu Bridge were conducted to evaluate the WSN's performance. Integral acceleration, temperature and tilt sensing modules were constructed to simplify the task of long-term environmental monitoring on this bridge, while a star topology was used to avoid collisions and reduce power consumption. We showed that, given sufficient power and additional power amplifier, the WSN can successfully be active for more than 7 days and satisfy the half bridge 120-meter transmission requirement. The time and frequency responses of cables shocked by external force and temperature variations around cables in one day were recorded and analyzed. Finally, guidelines on power characterization of the WSN platform and selection of acceleration sensors for structural health monitoring applications were given.

N-Acyl-Homoserine Lactone Quorum Sensing Switch from Acidogenesis to Solventogenesis during the Fermentation Process in Serratia marcescens MG1

  • Jin, Wensong;Lin, Hui;Gao, Huifang;Guo, Zewang;Li, Jiahuan;Xu, Quanming;Sun, Shujing;Hu, Kaihui;Lee, Jung-Kul;Zhang, Liaoyuan
    • Journal of Microbiology and Biotechnology
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    • 제29권4호
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    • pp.596-606
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    • 2019
  • N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ${\Delta}swrI$ with or without supplementing exogenous N-hexanoyl-L-homoserine lactone ($C_6-HSL$) were systematically investigated. The results showed that swrI disruption resulted in rapid pH drops from 7.0 to 4.8, which could be restored to wild type by supplementing $C_6-HSL$. Furthermore, fermentation product analysis indicated that ${\Delta}swrI$ could lead to obvious accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis products by ${\Delta}swrI$ appeared to noticeably decrease by an approximate 30% for acetoin during 32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to those by wild type. Interestingly, the excess acetic acid produced could be removed in an AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to determine the mRNA expression levels of genes responsible for acidogenesis and solventogenesis and showed consistent results with those of product synthesis. Finally, by close examination of promoter regions of the analyzed genes, four putative luxI box-like motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase, ${\alpha}$-acetolactate decarboxylase, and Lys-like regulator. The information from this study provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to solventogenesis in S. marcescens MG1.